Investigation of the potential protective effect of epigallocatechin gallate on paclitaxel-induced nerve injury
Abstract
Aim: Oxidative stress caused by untargeted anticancer drugs in tissues causes serious side effects such as cardiotoxicity, neurotoxicity, and hepatotoxicity. Paclitaxel is one of the taxane-derived chemotherapeutic drugs used in the treatment of solid tumors. Such chemotherapeutics are known to cause nerve damage. Epigallocatechin gallate (EGCG), which is the most active component of catechins in tea, is an important antioxidant and has an important role in ensuring DNA stability and healthy life. In this study, possible protective effects of epigallocatechin gallate against paclitaxel-induced oxidative stress were investigated. Method: 32 rats forming our study groups; divided into 4 groups with 8 rats in each group. As a result of the experimental protocol, the animals were sacrificed and sciatic nerve tissue was isolated. After nervous tissue homogenization, superoxide dismutase (SOD), catalase (KAT) enzyme activities and glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) levels were examined. Results: According to the data obtained, paclitaxel administration decreased SOD, CAT activity and GSH levels and increased MDA levels, however, EGCG treatment increased SOD, CAT activity and GSH levels and decreased MDA levels. Conclusion: As a result of our study, it was shown that EGCG can prevent the oxidative stress caused by paclitaxel in sciatic nerve tissue.
References
- Saraç, Ö. (2010). (-)-Epigallokateşin Gallat (EGCG) ve Kafeik Asit Fenetil Esterin (CAPE) Genotoksik ve Antigenotoksik Etkileri. Yüksek Lisans Tezi, Gazi Üniversitesi, Ankara.
- Wani, M.C., Taylor, H.L., Wall, M.E., Coggon, P., and McPhail, A.T. Plant Antitumor Agents. VI. The Isolation and Structure of Taxol, A Novel Antileukemic and Antitumor Agent from Taxus Brevifolia. J. Am. Chem. Soc,1971; 93(9):2325-2327.
- Arbuck, S.G., and Blaylock, B.A. Taxol: Clinical Results and Current Issues in Suffness M (ed.), Taxol: Science and Applications, CRC Press, Boca Raton, Florida, 1995;379-415.
- USPDI, 19th Ed., Micromedex Inc., Syracuse Way (1999).
- Schiff, P.B., Fant, J., andHorwitz, S.B. Promotion of Microtubule Assembly In Vitro by Taxol. Nature, 1979;277:665-667.
- Ringel, I., and Horwitz, S.B. Studies with RP 56976 (Taxotere): A Semisynthetic Analogue of Taxol. J. Natl. Cancer Inst., 1991;83:288-91.
- Gülen Akgün, H. (2016). Kurkumin-Oksim Bileşiğinin Sentezi, Karakterizasyonu ve Antioksidan Özelliklerinin Değerlendirilmesi. Yüksek Lisans Tezi, İstanbul Üniversitesi, İstanbul.
- Yagi K. Simple procedure for specific enzyme of lipid hydroperoxides in serum or plasma. Methods Mol Biol 1998; 108: 107-110.
- Sun Y, Oberley LW, Ying L. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500.
- Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121-126.
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1961; 193: 265-275.
- Guevara, I.; Iwanejko, J.; Dembinska-Kiec, A.; Pankiewicz, J.; Wanat, A.; Anna, P.; Golabek, I.; Bartus, S.; Malczewska-Malec, M.; Szczudlik, A. Determination of nitrite/nitrate in human biological material by the simple Griess reaction. Clin. Chim. Acta 1998, 274, 177–188.
- Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882-888.
- Fumaria officinalis’un antioksidan aktivitesinin belirlenmesi. Berna Özenç (Yüksek Lisans tezi), Kimya Anabilim Dalı Selcuk Üniversitesi.
- Li, S., Tan, H.-Y., Wang, N., Zhang, Z.-J., Lao, L., Wong, C.-W., & Feng, Y. (2015). The Role of Oxidative Stress and Antioxidants in Liver Diseases. International Journal of Molecular Sciences, 16(11), 26087–26124.
- Chu Q, Vincent M, Logan D, Mackay J A, Evans W K, Lung Cancer Disease Site Group. Taxanes as first-line therapy for advanced non-small cell lung cancer: a systematic review and practice guideline. Lung Cancer 2005; 50(3): 355-374.
- Pieniążek, A., Czepas, J., Piasecka-Zelga, J., Gwoździński, K., & Koceva-Chyła, A. (2013). Oxidative stress induced in rat liver by anticancer drugs doxorubicin, paclitaxel and docetaxel. Advances in medical sciences, 58(1), 104-111.
- Liebmann, J., Cook, J. A., Fisher, J., Teague, D., & Mitchell, J. B. (1994). In vitro studies of Taxol as a radiation sensitizer in human tumor cells. JNCI: Journal of the National Cancer Institute, 86(6), 441-446.
- Hadzic, T., Aykin-Burns, N., Zhu, Y., Coleman, M. C., Leick, K., Jacobson, G. M., & Spitz, D. R. (2010). Paclitaxel combined with inhibitors of glucose and hydroperoxide metabolism enhances breast cancer cell killing via H2O2-mediated oxidative stress. Free Radical Biology and Medicine, 48(8), 1024-1033.
- Powis, G., Briehl, M., & Oblong, J. (1995). Redox signalling and the control of cell growth and death. Pharmacology & therapeutics, 68(1), 149-173.
- Alexandre, J., Batteux, F., Nicco, C., Chéreau, C., Laurent, A., Guillevin, L., & Goldwasser, F. (2006). Accumulation of hydrogen peroxide is an early and crucial step for paclitaxel‐induced cancer cell death both in vitro and in vivo. International journal of cancer, 119(1), 41-48.
- Mir, O., Alexandre, J., Tran, A., Durand, J. P., Pons, G., Treluyer, J. M., & Goldwasser, F. (2009). Relationship between GSTP1 Ile105Val polymorphism and docetaxel-induced peripheral neuropathy: clinical evidence of a role of oxidative stress in taxane toxicity. Annals of oncology, 20(4), 736-740.
- Sun, H., Guo, X., Wang, Z., Wang, P., Zhang, Z., Dong, J., Cai, W. (2019). Alphalipoic Acid Prevents Oxidative Stress and Peripheral Neuropathy in Nab-Paclitaxel-Treated Rats through the Nrf2 Signalling Pathway. Oxidative medicine and cellular longevity, 2019.
- He, Y., Tan, D., Mi, Y., Bai, B., Jiang, D., Zhou, X., & Ji, S. (2017). Effect of epigallocatechin-3-gallate on acrylamide-induced oxidative stress and apoptosis in PC12 cells. Human & experimental toxicology, 36(10), 1087-1099.
- Ye, Q, Ye, L, Xu, X. Epigallocatechin-3-gallate suppresses 1-methyl-4-phenyl-pyridine-induced oxidative stress in PC12 cells via the SIRT1/PGC-1alpha signaling pathway. BMC 2012; 12: 82.
Paklitaksel kaynaklı sinir hasarında epigallokateşin gallatın potansiyel koruyucu etkisinin incelenmesi
Abstract
Amaç: Hedeflenmemiş antikanser ilaçlardan kaynaklı oksidatif stres çeşitli dokularda kardiyotoksisite, nörotoksisite ve hepatotoksisite gibi önemli yan etkilere neden olmaktadır. Paklitaksel, solid tümörlerin tedavisinde kullanılan taksan türevi kemoterapötik ilaçlardan biridir. Bu tür kemoterapötiklerin sinir hasarı oluşturduğu bilinmektedir. Çaydabulunan kateşinlerin en aktif bileşeni olan epigallokateşin gallat (EGCG) önemli bir antioksidan olup, DNA stabilitesinin sağlanılması ve sağlıklı yaşamda önemli bir role sahiptir. Çalışmamızda, paklitaksel ile oluşturulmuş oksidatif strese karşı epigallokateşin gallatın olası koruyucu etkileri incelenmiştir. Yöntem: Çalışma gruplarımızı oluşturan 32 sıçan; her grupta 8 sıçan olacak şekilde 4 gruba ayrılmıştır. Deney protokolünün sonunda sıçanlar sakrifiye edilerek siyatik sinir dokusu izole edilmiştir. Sinir dokusu homojenize edildikten sonra süperoksitdismutaz (SOD), katalaz (KAT) enzim aktiviteleri ileglutatyon (GSH), malondialdehid (MDA) ve nitrik oksit (NO) seviyeleri incelenmiştir. Bulgular: Elde edilen verilere göre paklitaksel uygulamasının SOD, KAT aktivitesi ve GSH düzeylerini azalttığı MDA seviyesini artırdığı, bununla birlikte EGCG tedavisinin SOD, KAT aktivitesi ve GSH düzeylerini arttırdığı ve MDA seviyesini azalttığı bulunmuştur. Sonuç: Çalışmamızın sonucunda EGCG’in paklitakselin siyatik sinir dokusunda neden olduğu oksidatif stresi engelleyebileceği gösterilmiştir.
References
- Saraç, Ö. (2010). (-)-Epigallokateşin Gallat (EGCG) ve Kafeik Asit Fenetil Esterin (CAPE) Genotoksik ve Antigenotoksik Etkileri. Yüksek Lisans Tezi, Gazi Üniversitesi, Ankara.
- Wani, M.C., Taylor, H.L., Wall, M.E., Coggon, P., and McPhail, A.T. Plant Antitumor Agents. VI. The Isolation and Structure of Taxol, A Novel Antileukemic and Antitumor Agent from Taxus Brevifolia. J. Am. Chem. Soc,1971; 93(9):2325-2327.
- Arbuck, S.G., and Blaylock, B.A. Taxol: Clinical Results and Current Issues in Suffness M (ed.), Taxol: Science and Applications, CRC Press, Boca Raton, Florida, 1995;379-415.
- USPDI, 19th Ed., Micromedex Inc., Syracuse Way (1999).
- Schiff, P.B., Fant, J., andHorwitz, S.B. Promotion of Microtubule Assembly In Vitro by Taxol. Nature, 1979;277:665-667.
- Ringel, I., and Horwitz, S.B. Studies with RP 56976 (Taxotere): A Semisynthetic Analogue of Taxol. J. Natl. Cancer Inst., 1991;83:288-91.
- Gülen Akgün, H. (2016). Kurkumin-Oksim Bileşiğinin Sentezi, Karakterizasyonu ve Antioksidan Özelliklerinin Değerlendirilmesi. Yüksek Lisans Tezi, İstanbul Üniversitesi, İstanbul.
- Yagi K. Simple procedure for specific enzyme of lipid hydroperoxides in serum or plasma. Methods Mol Biol 1998; 108: 107-110.
- Sun Y, Oberley LW, Ying L. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500.
- Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121-126.
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1961; 193: 265-275.
- Guevara, I.; Iwanejko, J.; Dembinska-Kiec, A.; Pankiewicz, J.; Wanat, A.; Anna, P.; Golabek, I.; Bartus, S.; Malczewska-Malec, M.; Szczudlik, A. Determination of nitrite/nitrate in human biological material by the simple Griess reaction. Clin. Chim. Acta 1998, 274, 177–188.
- Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882-888.
- Fumaria officinalis’un antioksidan aktivitesinin belirlenmesi. Berna Özenç (Yüksek Lisans tezi), Kimya Anabilim Dalı Selcuk Üniversitesi.
- Li, S., Tan, H.-Y., Wang, N., Zhang, Z.-J., Lao, L., Wong, C.-W., & Feng, Y. (2015). The Role of Oxidative Stress and Antioxidants in Liver Diseases. International Journal of Molecular Sciences, 16(11), 26087–26124.
- Chu Q, Vincent M, Logan D, Mackay J A, Evans W K, Lung Cancer Disease Site Group. Taxanes as first-line therapy for advanced non-small cell lung cancer: a systematic review and practice guideline. Lung Cancer 2005; 50(3): 355-374.
- Pieniążek, A., Czepas, J., Piasecka-Zelga, J., Gwoździński, K., & Koceva-Chyła, A. (2013). Oxidative stress induced in rat liver by anticancer drugs doxorubicin, paclitaxel and docetaxel. Advances in medical sciences, 58(1), 104-111.
- Liebmann, J., Cook, J. A., Fisher, J., Teague, D., & Mitchell, J. B. (1994). In vitro studies of Taxol as a radiation sensitizer in human tumor cells. JNCI: Journal of the National Cancer Institute, 86(6), 441-446.
- Hadzic, T., Aykin-Burns, N., Zhu, Y., Coleman, M. C., Leick, K., Jacobson, G. M., & Spitz, D. R. (2010). Paclitaxel combined with inhibitors of glucose and hydroperoxide metabolism enhances breast cancer cell killing via H2O2-mediated oxidative stress. Free Radical Biology and Medicine, 48(8), 1024-1033.
- Powis, G., Briehl, M., & Oblong, J. (1995). Redox signalling and the control of cell growth and death. Pharmacology & therapeutics, 68(1), 149-173.
- Alexandre, J., Batteux, F., Nicco, C., Chéreau, C., Laurent, A., Guillevin, L., & Goldwasser, F. (2006). Accumulation of hydrogen peroxide is an early and crucial step for paclitaxel‐induced cancer cell death both in vitro and in vivo. International journal of cancer, 119(1), 41-48.
- Mir, O., Alexandre, J., Tran, A., Durand, J. P., Pons, G., Treluyer, J. M., & Goldwasser, F. (2009). Relationship between GSTP1 Ile105Val polymorphism and docetaxel-induced peripheral neuropathy: clinical evidence of a role of oxidative stress in taxane toxicity. Annals of oncology, 20(4), 736-740.
- Sun, H., Guo, X., Wang, Z., Wang, P., Zhang, Z., Dong, J., Cai, W. (2019). Alphalipoic Acid Prevents Oxidative Stress and Peripheral Neuropathy in Nab-Paclitaxel-Treated Rats through the Nrf2 Signalling Pathway. Oxidative medicine and cellular longevity, 2019.
- He, Y., Tan, D., Mi, Y., Bai, B., Jiang, D., Zhou, X., & Ji, S. (2017). Effect of epigallocatechin-3-gallate on acrylamide-induced oxidative stress and apoptosis in PC12 cells. Human & experimental toxicology, 36(10), 1087-1099.
- Ye, Q, Ye, L, Xu, X. Epigallocatechin-3-gallate suppresses 1-methyl-4-phenyl-pyridine-induced oxidative stress in PC12 cells via the SIRT1/PGC-1alpha signaling pathway. BMC 2012; 12: 82.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Articles |
| Authors | |
| Publication Date | April 30, 2020 |
| Submission Date | January 27, 2020 |
| Acceptance Date | March 13, 2020 |
| Published in Issue | Year 2020 Volume: 13 Issue: 1 |
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