Abstract
Amaç: Deltametrin, tarımsal zararlılarla mücadelede yaygın olarak kullanılan tip II piretroid grubundan bir böcek öldürücü ilaçtır. Bu çalışmada deltametrinin sıçan siyatik siniri üzerine toksik etkisinin giderilmesinde E vitamininin koruyucu rolü olup olmadığının araştırılması amaçlandı.Yöntem: Çalışmada 28 adet Wistar albino sıçan kullanıldı. Sıçanlar grup I (kontrol), grup II (deltametrin), grup III (deltametrin+E vitamini) ve grup IV (deltametrin uygulamasından sonra 15 gün E vitamini) olarak rastgele dört gruba ayrıldı. Grup I’e ağız yoluyla sadece mısır yağı verildi. Grup II’ye 1.28 mg/kg dozunda deltametrin, grup III’e deltametrin+E vitamini kombinasyonu, grup IV’e ise deltametrin uygulaması tamamlandıktan sonra başlamak üzere E vitamini uygulaması yapıldı. Deltametrin ve E vitamini mısır yağında çözüldü. Uygulama 30 gün boyunca 48 saat aralıklarla tekrarlandı. Uygulamalardan sonra sıçanların bileşik sinir aksiyon potansiyelleri kayıtlanarak genlik ve latans ölçüldü. Kayıtlardan sonra siyatik sinirler izole edildi. İzole sinirlerde biyokimyasal teknikler kullanılarak süperoksit dismutaz, katalaz ve malondialdehit düzeyleri ölçüldü ve ışık mikroskobu kullanılarak yapısal değişiklikler saptandı.Bulgular: Grup II ve grup IV’de aksiyon potansiyelinin genliği kontrole göre önemli miktarda azaldı, malondialdehit düzeyi ise arttı. Her iki grupta miyelinli sinir liflerinin miyelin kılıflarında ve aksonlarında hasar izlendi. Grup III’de ise aksiyon potansiyelinin genliği ve süresi ile malondialdehit düzeyi açısından grup I’e göre önemli bir fark gözlenmedi. Bu grupta histolojik değerlendirmede de miyelinli sinir liflerinin çoğunlukla normal görünüme sahip oldukları belirlendi.Sonuç: Bu çalışmada deltametrinin sıçan siyatik sinirinde oksidatif strese yol açarak akson ve miyelin hasarı oluşturduğu, deltametrinle birlikte antioksidan E vitaminin verilmesinin bu hasarı önlediği, hasar oluştuktan sonra tedavi amaçlı E vitamini uygulanmasının ise hasarı kısmen azalttığı saptanmıştır.
Keywords
References
- Verschoyle RD, Aldridge WN. Structure-activity relation- ships of some pyrethroids in rats. Arch Toxical 1980;l45 (4):325-9.
- Hallenbeck WH, Cunningham-Burns KM. Pesticides and Human Health; 1at ed. New York: Springer-Verlag, : l 18. Meyer DA, Carter JM, Johnstone AF, Shafer TJ. Pyrethroid modulation of spontaneous neuronal excitability and neurotransmission in hippocampal neurons in culture. Neuratoxicalagy 2008;29(2):213-25.
- Soderlund DM, Knipple DC. The molecular biology of knockdown resistance to pyrethroid insecticides. Insect Biachem Mal Biol 2003;33(6):563-77.
- Ray DE. Pyrethroid insecticides: mechanisms of toxicity, systemic poisoning syndromes, paresthesia, and therapy. In: Krieger R, Doull J, Ecobichon D Eds: Handbook of pesticide toxicology, 2“d Ed., San Diego: Academic Press, :1289-303.
- Hildebrand ME, McRory JE, Snutch TP, Stea A. Mammalian voltage-gated calcium channels are potently blocked by the pyrethroid insecticide allethrin. J Pharma- col Exp Ther 2004;308(3):805-l3.
- Burr SA, Ray DE. Structure-activity and interaction effects of 14 different pyrethroids on voltage-gated chloride ion channels. Toxicol Sci 2004;77(2):341-6.
- Gray AJ. Pyrethroid structure-toxicity relationships in mammals. Neurataxicalogy 1985 ;6(2): 127-37.
- Vijverberg HP, van den Bercken J. Neurotoxicogical effects and the mode of action of pyrethroid insecticides. Crit Rev Toxical 1990;21(2):105-26. on rat brain microanatomy and cholinergic/dopaminergic neurochemistry. Brain Res 2009;1301:180-8. rat frontal cortex following acute in vivo exposure to the pyrethroid insecticides permethrin and deltamethrin. BMC Genamics 2008;9:546.
- & cytochrome C genes in rat cerebrum with deltamethrin induction. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2008;26(4):215-8. isolated sciatic nevre of a frog (Rana ridibunda). Comp
- Biochem Physiol C Pharmaeol Toxicol Endocrine ;118(1):97-103. Calore EE, Cavaliere MJ , Puga FR, Calore NM, Rosa AR, Weg R, Dias SS, Santos RP. Histologic peripheral nerve Dubey N, Khan AM, Raina R. Sub-acute deltamethrin and şuoride toxicity induced hepatic oxidative stress and Li HY, Shi N, Chen D, Dai ZH, Lu WH, Wang B, Li YR. Oxidative stress of deltamethrin on rat nervous system. Cheeseman KH, Slater TF. An introduction to free radical biochemistry. Br Med Bull 1993 ;49(3):48 1 -93
- YousefMI, Awad TI, Mohamed EH. Deltamethrin-induced oxidative damage and biochemical alterations in rat and its Aebi H. Catalase in vitro. Methods Enzymol 1 984; 105: 121-6. Sun Y, Oberley LW, Li Y. A simple method for clinical assay Yagi K. Simple procedure for specific enzyme of lipid hydroperoxides in serum or plasma. Methods Mol Biol Kimura J. Electrodiagnosis in disease of nerve and muscle: principles and practice. 2nd Ed., Philadelphia, USA, FA Davis Company, 1989: 373.
Abstract
References
- Verschoyle RD, Aldridge WN. Structure-activity relation- ships of some pyrethroids in rats. Arch Toxical 1980;l45 (4):325-9.
- Hallenbeck WH, Cunningham-Burns KM. Pesticides and Human Health; 1at ed. New York: Springer-Verlag, : l 18. Meyer DA, Carter JM, Johnstone AF, Shafer TJ. Pyrethroid modulation of spontaneous neuronal excitability and neurotransmission in hippocampal neurons in culture. Neuratoxicalagy 2008;29(2):213-25.
- Soderlund DM, Knipple DC. The molecular biology of knockdown resistance to pyrethroid insecticides. Insect Biachem Mal Biol 2003;33(6):563-77.
- Ray DE. Pyrethroid insecticides: mechanisms of toxicity, systemic poisoning syndromes, paresthesia, and therapy. In: Krieger R, Doull J, Ecobichon D Eds: Handbook of pesticide toxicology, 2“d Ed., San Diego: Academic Press, :1289-303.
- Hildebrand ME, McRory JE, Snutch TP, Stea A. Mammalian voltage-gated calcium channels are potently blocked by the pyrethroid insecticide allethrin. J Pharma- col Exp Ther 2004;308(3):805-l3.
- Burr SA, Ray DE. Structure-activity and interaction effects of 14 different pyrethroids on voltage-gated chloride ion channels. Toxicol Sci 2004;77(2):341-6.
- Gray AJ. Pyrethroid structure-toxicity relationships in mammals. Neurataxicalogy 1985 ;6(2): 127-37.
- Vijverberg HP, van den Bercken J. Neurotoxicogical effects and the mode of action of pyrethroid insecticides. Crit Rev Toxical 1990;21(2):105-26. on rat brain microanatomy and cholinergic/dopaminergic neurochemistry. Brain Res 2009;1301:180-8. rat frontal cortex following acute in vivo exposure to the pyrethroid insecticides permethrin and deltamethrin. BMC Genamics 2008;9:546.
- & cytochrome C genes in rat cerebrum with deltamethrin induction. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2008;26(4):215-8. isolated sciatic nevre of a frog (Rana ridibunda). Comp
- Biochem Physiol C Pharmaeol Toxicol Endocrine ;118(1):97-103. Calore EE, Cavaliere MJ , Puga FR, Calore NM, Rosa AR, Weg R, Dias SS, Santos RP. Histologic peripheral nerve Dubey N, Khan AM, Raina R. Sub-acute deltamethrin and şuoride toxicity induced hepatic oxidative stress and Li HY, Shi N, Chen D, Dai ZH, Lu WH, Wang B, Li YR. Oxidative stress of deltamethrin on rat nervous system. Cheeseman KH, Slater TF. An introduction to free radical biochemistry. Br Med Bull 1993 ;49(3):48 1 -93
- YousefMI, Awad TI, Mohamed EH. Deltamethrin-induced oxidative damage and biochemical alterations in rat and its Aebi H. Catalase in vitro. Methods Enzymol 1 984; 105: 121-6. Sun Y, Oberley LW, Li Y. A simple method for clinical assay Yagi K. Simple procedure for specific enzyme of lipid hydroperoxides in serum or plasma. Methods Mol Biol Kimura J. Electrodiagnosis in disease of nerve and muscle: principles and practice. 2nd Ed., Philadelphia, USA, FA Davis Company, 1989: 373.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | May 5, 2015 |
| Submission Date | May 4, 2015 |
| Published in Issue | Year 2014 Volume: 7 Issue: 1 |
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MEU Journal of Health Sciences Assoc was began to the publishing process in 2008 under the supervision of Assoc. Prof. Gönül Aslan, Editor-in-Chief, and affiliated to Mersin University Institute of Health Sciences. In March 2015, Prof. Dr. Caferi Tayyar Şaşmaz undertook the Editor-in Chief position and since then he has been in charge.
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