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Propolisin Antisitotoksik ve Antimutajenik Etkilerinin İnsan Lenfositlerinde İn Vitro Olarak Belirlenmesi

Year 2016, Volume: 16 Issue: 2, 38 - 46, 30.12.2016

Sinan Bayram Nesrin Ecem Bayram Yusuf Can Gerçek Kadriye Sorkun

Abstract

Propolis, koloniler için antiseptik bir çevre oluşturmak amacıyla bal arıları tarafından farklı bitkilerden toplanan, reçinemsi doğal bir üründür. Antik çağlardan beri alternatif tıpta terapötik bir ajan olarak kullanılmaktadır. Bu çalışmanın amacı Hakkari, Türkiye bölgesinden toplanan propolis ekstraktının AFBı’e (aflatoxin Bı) karşı insan lenfosit hücrelerinde in vitro olarak antisitotoksik ve antigenotoksik etkilerinin araştırılmasıdır. Propolisin kimyasal içeriği GC-MS (Gaz kromatografi – Kütle spektrometre) ile belirlenmiş ve yüksek oranda flavonoid içerdiği belirlenmiştir. Mutajenite test sonuçları AFB1’in kardeş kromatid değişimi (SCE) frekansını arttırdığını ve DNA hasarlarına neden olduğunu göstermiştir. Propolis ise in vitro ortamda AFBı’e karşı insan lenfositlerinde güçlü antimutajenik etki göstermiş ve SCE frekansını azaltmıştır. Buna ek olarak propolis’in sitotoksik ve antisitotoksik etkisi LDH (Laktat dehidrogenaz) salınım testi ile belirlenmiştir. Testler sonucunda propolisin aflatoksin B1’e karşı antisitotoksik ve antigenotoksik etkiye sahip olduğu görülmüştür.



 

Keywords

Aflatoksin Bı Propolis GC/MS analizi Kardeş Kromatid Değişimi (SCE) Testi LDH (laktat dehidrogenaz) salınım testi

References

  • MATTIUZ, B H; DUCAMP-COLLIN, M N; MATTİUZ, C F M, VİGNEAULT, C; MARQUES, K M; SAGOUA, W; MONTET, D (2015) Effect of propolis on postharvest control of anthracnose and quality parameters of ‘Kent’mango, Sci. Hortic. 184, 160-168.

Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro

Year 2016, Volume: 16 Issue: 2, 38 - 46, 30.12.2016

Sinan Bayram Nesrin Ecem Bayram Yusuf Can Gerçek Kadriye Sorkun

Abstract

Propolis is a natural product which is collected from different plants by honeybees to provide antiseptic environment for colonies.  It has been used as a therapeutical agent in alternative medicine since ancient times. The aim of this study was to investigate antimutagenic and anticytotoxic effects of propolis extract from Turkey’s Hakkari region against aflatoxin Bı (AFBı) on human lymphocytes in vitro. Chemical content of propolis extract which was determined by Gas Chromatography−Mass Spectrometry (GC-MS) and it was observed to contain high amounts of flavonoids. The mutagenicity test results showed that AFB1 caused DNA damages and increased sister chromatid exchange (SCE) frequency. Propolis reduced SCE frequency and showed strong antimutagenic effect against AFBı on human lymphocytes. In addition, Cytotoxic and anticytotoxic effect of propolis was examined by LDH (lactate dehydrogenase) leakage test.  Consequently, our findings showed that propolis had strong anticytotoxic and antigenotoxic properties against aflatoxin B1.



 

Keywords

Aflatoxin Bı Propolis; GC/MS analysis Sister Chromatid Exchange (SCE) Test LDH (lactate dehydrogenase) leakage assay

References

  • MATTIUZ, B H; DUCAMP-COLLIN, M N; MATTİUZ, C F M, VİGNEAULT, C; MARQUES, K M; SAGOUA, W; MONTET, D (2015) Effect of propolis on postharvest control of anthracnose and quality parameters of ‘Kent’mango, Sci. Hortic. 184, 160-168.
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Details

Subjects Structural Biology
Journal Section Articles
Authors

Sinan Bayram This is me

Nesrin Ecem Bayram

Yusuf Can Gerçek This is me

Kadriye Sorkun This is me

Publication Date December 30, 2016
Submission Date February 27, 2017
Published in Issue Year 2016 Volume: 16 Issue: 2

Cite

APA Bayram, S., Ecem Bayram, N., Gerçek, Y. C., Sorkun, K. (2016). Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro. Mellifera, 16(2), 38-46.
AMA Bayram S, Ecem Bayram N, Gerçek YC, Sorkun K. Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro. mellifera. December 2016;16(2):38-46.
Chicago Bayram, Sinan, Nesrin Ecem Bayram, Yusuf Can Gerçek, and Kadriye Sorkun. “Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro”. Mellifera 16, no. 2 (December 2016): 38-46.
EndNote Bayram S, Ecem Bayram N, Gerçek YC, Sorkun K (December 1, 2016) Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro. Mellifera 16 2 38–46.
IEEE S. Bayram, N. Ecem Bayram, Y. C. Gerçek, and K. Sorkun, “Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro”, mellifera, vol. 16, no. 2, pp. 38–46, 2016.
ISNAD Bayram, Sinan et al. “Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro”. Mellifera 16/2 (December 2016), 38-46.
JAMA Bayram S, Ecem Bayram N, Gerçek YC, Sorkun K. Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro. mellifera. 2016;16:38–46.
MLA Bayram, Sinan et al. “Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro”. Mellifera, vol. 16, no. 2, 2016, pp. 38-46.
Vancouver Bayram S, Ecem Bayram N, Gerçek YC, Sorkun K. Anticytotoxic and Antimutagenic Effects of Propolis on Human Lymphocytes In Vitro. mellifera. 2016;16(2):38-46.
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