Research Article
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Year 2019, Volume: 5 Issue: 2, 63 - 66, 28.08.2019
https://doi.org/10.19127/mbsjohs.544429

Abstract

References

  • Chang Y, Li J, Chen Y, Wei L, Yang X, Shi Y, et al. Autologous platelet-rich plasma promotes endometrial growth and improves pregnancy outcome during in vitro fertilization. Int J Clin Exp Med. 2015;8:1286-90.
  • Choi Y, Kim HR, Lim EJ, Park M, Yoon JA, Kim YS, et al. Integrative Analyses of Uterine Transcriptome and MicroRNAome Reveal Compromised LIF-STAT3 Signaling and Progesterone Response in the Endometrium of Patients with Recurrent/Repeated Implantation Failure (RIF). PLoS One. 2016;11:e0157696.
  • Collins JA, Crosignani PG. Unexplained infertility: a review of diagnosis, prognosis, treatment efficacy and management. Int J Gynaecol Obstet. 1992;39:267-75.
  • Custers IM, van Rumste MM, van der Steeg JW, van Wely M, Hompes PG, Bossuyt P, et al. Long-term outcome in couples with unexplained subfertility and an intermediate prognosis initially randomized between expectant management and immediate treatment. Hum Reprod. 2012;27:444-50.
  • Dhillon RS, Schwarz EM, Maloney MD. Platelet-rich plasma therapy - future or trend?. Arthritis Res Ther. 2012;14:219. Evers JL. Female subfertility. Lancet. 2002;360:151-9.
  • Garcia-Velasco JA, Acevedo B, Alvarez C, Alvarez M, Bellver J, Fontes J, et al . Strategies to manage refractory endometrium: state of the art in 2016. Reprod Biomed Online. 2016;32:474-89.
  • Green CJ, Fraser ST, Day ML. Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro. Hum Reprod. 2015;30:284-98.
  • Margalioth EJ, Ben-Chetrit A, Gal M, Eldar-Geva T. Investigation and treatment of repeated implantation failure following IVF-ET. Hum Reprod. 2006;21:3036-43.
  • Nazari L, Salehpour S, Hoseini S, Zadehmodarres S, Ajori L. Effects of autologous platelet-rich plasma on implantation and pregnancy in repeated implantation failure: A pilot study. Int J Reprod Biomed. 2016;14:625-8.
  • Practice Committee of American Society for Reproductive Medicine (ASRM). Definitions of infertility and recurrent pregnancy loss. Fertil Steril. 2008;90:S60
  • Ronci C, Ferraro AS, Lanti A, Missiroli F, Sinopoli S, Del Proposto G, et al. Platelet-rich plasma as treatment for persistent ocular epithelial defects. Transfus Apher Sci. 2015;52:300-4.
  • Sak ME, Gul T, Evsen MS, Soydinc HE, Sak S, Ozler A, et al. Fibroblast growth factor-1 expression in the endometrium of patients with repeated implantation failure after in vitro fertilization. Eur Rev Med Pharmacol Sci. 2013;17:398-402.
  • Sfakianoudis K, Simopoulou M, Nitsos N, Rapani A, Pantou A, Vaxevanoglou T, et al. A Case Series on Platelet-Rich Plasma Revolutionary Management of Poor Responder Patients. Gynecol Obstet Invest. 2018;1-8.
  • van Eekelen R, Tjon-Kon-Fat RI, Bossuyt PMM, van Geloven N, Eijkemans MJC, Bensdorp AJ, et al. Natural conception rates in couples with unexplained or mild male subfertility scheduled for fertility treatment: a secondary analysis of a randomized controlled trial. Hum Reprod. 2018;33:919-23.
  • Veltman-Verhulst SM, Hughes E, Ayeleke RO, Cohlen BJ.. Intra-uterine insemination for unexplained subfertility. Cochrane Database Syst Rev. 2016;2:Cd001838.
  • Zadehmodarres S, Salehpour S, Saharkhiz N, Nazari L. Treatment of thin endometrium with autologous platelet-rich plasma: a pilot study. JBRA Assist Reprod. 2017;21:54-6.

Effects of Autologous Platelet-Rich Plasma on Endometrium Thickness and Pregnancy Rates During Intrauterine Insemination

Year 2019, Volume: 5 Issue: 2, 63 - 66, 28.08.2019
https://doi.org/10.19127/mbsjohs.544429

Abstract



Objective: Evaluation of the effect of platelet-rich plasma (PRP)
on the endometrium and pregnancy outcomes in patients undergoing insemination
due to unexplained infertility.



Methods: 24 patients who were admitted to the clinic due to
unexplained infertility, analyzed retrospectively between March 2018 and
October 2018. Gonadotropin induction was initiated on day 3 of the cycle for
follicular growth. Human chorionic gonadotropin (hCG) was applied for ovulation
induction at the point that at least 1 follicle that is over 16 mm was detected
by transvaginal ultrasound.
17.5 ml of
blood from the patient's venous system was drawn for the preparation of the PRP
which includes 4-5 times more platelets than regular blood. PRP was
administered to 12 patients (Group 1) on the hCG day, while hCG was solely
administered to the other group (Group 2) and both groups were inseminated 36
hours later.



Results: The demographic properties of all patients were
determined as follows: mean age; 29.13 years old (±3.4), mean infertility
period; 1.96 years (±1.08), mean ovulation induction period; 7.92 days (±1.76),
mean antral follicle count; 14.54 (±6.56), mean dominant follicle count; 2.04
(±0.75). Although there was no significant difference between the groups in
terms of clinical pregnancy (3/12 vs 2/12, p: 0.623), the change in endometrial
thickness was significantly higher in the PRP administered group (1.95 mm vs
0.44 mm, p< 0.001).



Conclusion: PRP application before the insemination seems promising for the
preparation of the endometrium in patients having an inadequate endometrial thickness
or in patients experiencing recurrent implantation failure.




References

  • Chang Y, Li J, Chen Y, Wei L, Yang X, Shi Y, et al. Autologous platelet-rich plasma promotes endometrial growth and improves pregnancy outcome during in vitro fertilization. Int J Clin Exp Med. 2015;8:1286-90.
  • Choi Y, Kim HR, Lim EJ, Park M, Yoon JA, Kim YS, et al. Integrative Analyses of Uterine Transcriptome and MicroRNAome Reveal Compromised LIF-STAT3 Signaling and Progesterone Response in the Endometrium of Patients with Recurrent/Repeated Implantation Failure (RIF). PLoS One. 2016;11:e0157696.
  • Collins JA, Crosignani PG. Unexplained infertility: a review of diagnosis, prognosis, treatment efficacy and management. Int J Gynaecol Obstet. 1992;39:267-75.
  • Custers IM, van Rumste MM, van der Steeg JW, van Wely M, Hompes PG, Bossuyt P, et al. Long-term outcome in couples with unexplained subfertility and an intermediate prognosis initially randomized between expectant management and immediate treatment. Hum Reprod. 2012;27:444-50.
  • Dhillon RS, Schwarz EM, Maloney MD. Platelet-rich plasma therapy - future or trend?. Arthritis Res Ther. 2012;14:219. Evers JL. Female subfertility. Lancet. 2002;360:151-9.
  • Garcia-Velasco JA, Acevedo B, Alvarez C, Alvarez M, Bellver J, Fontes J, et al . Strategies to manage refractory endometrium: state of the art in 2016. Reprod Biomed Online. 2016;32:474-89.
  • Green CJ, Fraser ST, Day ML. Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro. Hum Reprod. 2015;30:284-98.
  • Margalioth EJ, Ben-Chetrit A, Gal M, Eldar-Geva T. Investigation and treatment of repeated implantation failure following IVF-ET. Hum Reprod. 2006;21:3036-43.
  • Nazari L, Salehpour S, Hoseini S, Zadehmodarres S, Ajori L. Effects of autologous platelet-rich plasma on implantation and pregnancy in repeated implantation failure: A pilot study. Int J Reprod Biomed. 2016;14:625-8.
  • Practice Committee of American Society for Reproductive Medicine (ASRM). Definitions of infertility and recurrent pregnancy loss. Fertil Steril. 2008;90:S60
  • Ronci C, Ferraro AS, Lanti A, Missiroli F, Sinopoli S, Del Proposto G, et al. Platelet-rich plasma as treatment for persistent ocular epithelial defects. Transfus Apher Sci. 2015;52:300-4.
  • Sak ME, Gul T, Evsen MS, Soydinc HE, Sak S, Ozler A, et al. Fibroblast growth factor-1 expression in the endometrium of patients with repeated implantation failure after in vitro fertilization. Eur Rev Med Pharmacol Sci. 2013;17:398-402.
  • Sfakianoudis K, Simopoulou M, Nitsos N, Rapani A, Pantou A, Vaxevanoglou T, et al. A Case Series on Platelet-Rich Plasma Revolutionary Management of Poor Responder Patients. Gynecol Obstet Invest. 2018;1-8.
  • van Eekelen R, Tjon-Kon-Fat RI, Bossuyt PMM, van Geloven N, Eijkemans MJC, Bensdorp AJ, et al. Natural conception rates in couples with unexplained or mild male subfertility scheduled for fertility treatment: a secondary analysis of a randomized controlled trial. Hum Reprod. 2018;33:919-23.
  • Veltman-Verhulst SM, Hughes E, Ayeleke RO, Cohlen BJ.. Intra-uterine insemination for unexplained subfertility. Cochrane Database Syst Rev. 2016;2:Cd001838.
  • Zadehmodarres S, Salehpour S, Saharkhiz N, Nazari L. Treatment of thin endometrium with autologous platelet-rich plasma: a pilot study. JBRA Assist Reprod. 2017;21:54-6.
There are 16 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research articles
Authors

Mehmet Sipahi

Publication Date August 28, 2019
Published in Issue Year 2019 Volume: 5 Issue: 2

Cite

Vancouver Sipahi M. Effects of Autologous Platelet-Rich Plasma on Endometrium Thickness and Pregnancy Rates During Intrauterine Insemination. Mid Blac Sea J Health Sci. 2019;5(2):63-6.

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