Research Article
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Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa

Year 2023, Volume: 12 Issue: 2, 244 - 251, 30.06.2023

Emine Arslan Elif Gülbahçe Mutlu

https://doi.org/10.33714/masteb.1281335

Abstract

Besides traditional methods based on morphological characters, electrophoretic methods such as SDS-PAGE are preferred by taxonomists to make the right decision in the species identification process. In addition, the effect of environmental factors, such as pH, salinity, heat, and temperature on protein profiles are essential in various studies. In this study, we aimed to determine the degree of relationship in some fish species, such as Squalius lepidus, Cyprinus carpio, Carassius gibelio, Pseudophoxinus anatolicus, Tinca tinca, Alburnus orontis, Scardinius erythrophthalmus, Capoeta capoeta, Vimba vimba, Sander lucioperca living in Beyşehir, Suğla lakes and Apa Dam by SDS-PAGE method, and to examine seasonal differences by evaluating the effect of hot/cold water on protein profiles in fish. Although there were common major protein bands in all fish species studied, the presence of species-specific minor protein bands led to the separation of the species. The same fish species distributed in different lakes and dams were different both in minor bands, and changes in protein profiles were observed consequently on the same fish species synthesizing different proteins in different seasons. The data obtained from this study can contribute to systematic classification studies of fish.

Keywords

Fish Protein profiles Relationship degree SDS-PAGE Seasonal changes

Supporting Institution

Selcuk University Scientific Research Projects Coordinatorship

Project Number

14401063

Thanks

We thank you the Scientific Research Projects Coordinatorship of Selcuk University (Grant number: 14401063).

References

  • Abolfathi, M., Akbarzadeh, A., Hajimoradloo, A., Joshaghani, H. R., & Ross, N. W. (2022). Seasonal variations in the skin epidermal structure and mucosal immune parameters of rainbow trout skin (Oncorhynchus mykiss) at different stages of farming. Fish & Shellfish Immunology, 127, 965-974. https://doi.org/10.1016/j.fsi.2022.07.028
  • Berrini, A., Tepedino, V., Borromeo, V., & Secchi, C. (2006). Identification of freshwater fish commercially labelled “perch” by isoelectric focusing and two-dimensional electrophoresis. Food Chemistry, 96(1), 163-168. https://doi.org/10.1016/j.foodchem.2005.04.007
  • Crick, F. H. C. (1963). On the genetic code. Science, 139(3554), 461-464. https://doi.org/10.1126/science.139.3554.461
  • Durand, J. D., Tsigenopoulos, C. S., Ünlü, E., & Berrebi, P. (2002). Phylogeny and biogeography of the Family Cyprinidae in the Middle East inferred from cytochrome b DNA-evolutionary significance of this region. Molecular Phylogenetics and Evolution, 22(1), 91-100. https://doi.org/10.1006/mpev.2001.1040
  • Etienne, M., Jerome, M., Fleurence, J., Rehbein, H., Kündiger, R., Mendes, R., Costa, H., & Martinez, I. (2001). Species identification of formed fishery products and high pressure treated fish by electrophoresis: A collaborative study. Food Chemistry, 72(1), 105-112. https://doi.org/10.1016/S0308-8146(00)00205-3
  • Fowler, J. A. (1970). Control of vertebral number in teleosts-an embryological problem. The Quarterly Review of Biology, 45(2), 148-167.
  • Ganai, F. A., Wani, S., Ahmad, S., Yousuf, A. R., & Tripathi, N. K. (2014). Coupled biochemical genetic and karyomorphological analyses for taxonomic classification-A case study of Schizothorax species complex (Teleostei: Cyprinidae). African Journal of Biotechnology, 13(15), 1623-1630. https://doi.org/10.5897/AJB2013.13370
  • He, S., Mayden, R. L., Wang, X., Wang, W., Tang, K. L., Chen, W. J., & Chen, Y. (2008). Molecular phylogenetics of the family Cyprinidae (Actinopterygii: Cypriniformes) as evidenced by sequence variation in the first intron of S7 ribosomal protein-coding gene: Further evidence from a nuclear gene of the systematic chaos in the family. Molecular Phylogenetics and Evolution, 46(3), 818-829. https://doi.org/10.1016/j.ympev.2007.06.001
  • Hofmann, K. (1987). Fundamental problems in identifying the animal species of muscle meat using electrophoretic methods. Fleischwirtsch, 67, 820-828.
  • Hua, C. J., Zhang, D., Zou, H. Li, M., Jakovlić, I., Wu, S. G., Wang, G. T., & Li, W. X. (2019). Morphology is not a reliable taxonomic tool for the genus Lernaea: Molecular data and experimental infection reveal that L. cyprinacea and L. cruciata are conspecific. Parasites & Vectors, 12, 579. https://doi.org/10.1186/s13071-019-3831-y
  • Ihuţ, A., Răducu, C., Cocan, D., Munteanu, C., Luca, I., Uiuiu, P., Mireşan, V., Lațiu, C., & Rus, V. (2020) Seasonal variation of blood biomarkers in huchen, Hucho hucho (Actinopterygii: Salmoniformes: Salmonidae) reared in captivity. Acta Ichthyologica et Piscatoria, 50(4), 381-390. https://doi.org/10.3750/AIEP/02836
  • Imoto, J. M., Saitoh, K., Sasaki, T., Yonezawa, T., Adachi, J., Kartavtsev, Y. P., Miya M., Nishida, M., & Hanzawa, N. (2013). Phylogeny and biogeography of highly diverged freshwater fish species (Leuciscinae, Cyprinidae, Teleostei) inferred from mitochondrial genome analysis. Gene, 514(2), 112-124. https://doi.org/10.1016/j.gene.2012.10.019
  • Kuru, M. (2004). Türkiye iç su balıklarının son sistematik durumu [Recent systematic status of inland water fishes of Turkey]. Gazi Üniversitesi Gazi Eğitim Fakültesi Dergisi, 24(3), 1-21.
  • Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of Bacteriophage T4. Nature, 277(5259), 680-684. https://doi.org/10.1038/227680a0
  • Li, J., Wang, X., Kong, X., Zhao, K., He, S., & Mayden, R. L. (2008). Variation patterns of the mitochondrial 16S rRNA gene with secondary structure constraints and their application to phylogeny of cyprinine fishes (Teleostei: Cypriniformes). Molecular Phylogenetics and Evolution, 47(2), 472-487. https://doi.org/10.1016/j.ympev.2007.09.012
  • Martinez, I., Jakobsen, F. T., & Seppola, M. (2001). Requirements for the application of protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation. Electrophoresis, 22(8), 1526-1533. https://doi.org/10.1002/1522-2683(200105)22:8%3C1526::aid-elps1526%3E3.0.co;2-3
  • Muhammad, O. I., Mahmoud, U. M., Fazio, F., & Sayed, A. E. H. (2018). SDS-PAGE technique as biomarker for fish toxicological studies. Toxicology Reports, 5, 905–909. https://doi.org/10.1016/j.toxrep.2018.08.020
  • Nei, M. (1978). Estimation of average heterozygosity and genetic distances from a small number of individuals. Genetics, 89(3), 583-590. https://doi.org/10.1093/genetics/89.3.583
  • Nelson, J. S. (1994). Fishes of the World. John Wiley and Sons, Inc.
  • Nirenberg, N. W., Matthaei, J. H., Jones, O. W., Martin, R. G., & Barondes, S. H. (1963). Approximation of genetic code via cell free protein synthesis detected by template RNA. Federation Proceedings, 22, 55-61.
  • Ochoa, S. (1963). Synthetic polynucleotides and genetic code. Federation Proceedings, 22, 62-74.
  • Şalcıoğlu, A., Gubili, C., Krey, G., Sönmez, A. Y., & Bilgin, R. (2020). Phylogeography and population dynamics of the Eastern Mediterranean whiting (Merlangius merlangus) from the Black Sea, the Turkish Straits System, and the North Aegean Sea. Fisheries Research, 229, 105614. https://doi.org/10.1016/j.fishres.2020.105614
  • Tang, K. L., Agnew, M. K., Chen, W. J., Hirt, M. V., Raley, M. E., Sado, T., Schneider, L. M., Yang, L., Bart, H. L., He, S., Liu, H., Miya, M., Saitoh, K., Simons, A. M., Wooda, R. M., & Mayden, R. L. (2011). Phylogeny of the gudgeons (Teleostei: Cyprinidae: Gobioninae). Molecular Phylogenetics and Evolution, 61(1), 103-124. https://doi.org/10.1016/j.ympev.2011.05.022
  • Tang, K. L., Agnew, M. K., Hirt, M. V., Sado, T., Schneider, L. M., Freyhof, J., Sulaiman, Z., Swartz, E., Vidthayanon, C., Miya, M., Saitoh, K., Simons, A. M., Wood, R. M., & Mayden, R. L. (2010). Systematics of the subfamily Danioninae (Teleostei: Cypriniformes: Cyprinidae). Molecular Phylogenetics and Evolution, 57(1), 189-214. https://doi.org/10.1016/j.ympev.2010.05.021
  • Theophilus, J., & Rao, P. R. (1998). Electrophoretic studies on the serum proteins of the three species of genus Channa. Indian Journal of Fisheries, 35(4), 294-297.
  • Tokur, B., & Kandemir, S. (2008). Dondurulmuş balıklarda farklı çözündürme şekillerinin protein kalitesine olan etkileri [The effects of different thawing methods on protein quality of frozen fish]. Journal of FisheriesSciences.com, 2(1), 100-106. https://doi.org/10.3153/jfscom.2008010
  • Yılmaz, M., Çiğremiş, Y., Türköz, Y., & Gaffaroğlu, M. (2005). A taxonomic study on Orthrias insignis euphraticus (Banarescu and Nalbant, 1964) and Cyprinion macrostomus (Heckel, 1843) by sarcoplasmic protein electrophoresis. Gazi University Journal of Science, 18(1), 61-68.
  • Yılmaz, M., Türköz, Y., Erdemli, A. Ü., Kalkan, E., & Çiğremiş, Y. (2000). A taxonomical study on electrophoretic pattern of blood serum protein of some fish of Karakaya Dam Lake. Eurasian Journal of Veterinary Sciences, 16(1), 89-92.
  • Yilmaz, M., Yilmaz, H. R., & Alas, A. (2007). An electrophoretic taxonomic study on serum proteins of Acanthobrama marmid, Leuciscus cephalus, and Chondrostoma regium. EurAsian Journal of BioSciences, 3, 22-27.

Year 2023, Volume: 12 Issue: 2, 244 - 251, 30.06.2023

Emine Arslan Elif Gülbahçe Mutlu

https://doi.org/10.33714/masteb.1281335

Abstract

Project Number

14401063

References

  • Abolfathi, M., Akbarzadeh, A., Hajimoradloo, A., Joshaghani, H. R., & Ross, N. W. (2022). Seasonal variations in the skin epidermal structure and mucosal immune parameters of rainbow trout skin (Oncorhynchus mykiss) at different stages of farming. Fish & Shellfish Immunology, 127, 965-974. https://doi.org/10.1016/j.fsi.2022.07.028
  • Berrini, A., Tepedino, V., Borromeo, V., & Secchi, C. (2006). Identification of freshwater fish commercially labelled “perch” by isoelectric focusing and two-dimensional electrophoresis. Food Chemistry, 96(1), 163-168. https://doi.org/10.1016/j.foodchem.2005.04.007
  • Crick, F. H. C. (1963). On the genetic code. Science, 139(3554), 461-464. https://doi.org/10.1126/science.139.3554.461
  • Durand, J. D., Tsigenopoulos, C. S., Ünlü, E., & Berrebi, P. (2002). Phylogeny and biogeography of the Family Cyprinidae in the Middle East inferred from cytochrome b DNA-evolutionary significance of this region. Molecular Phylogenetics and Evolution, 22(1), 91-100. https://doi.org/10.1006/mpev.2001.1040
  • Etienne, M., Jerome, M., Fleurence, J., Rehbein, H., Kündiger, R., Mendes, R., Costa, H., & Martinez, I. (2001). Species identification of formed fishery products and high pressure treated fish by electrophoresis: A collaborative study. Food Chemistry, 72(1), 105-112. https://doi.org/10.1016/S0308-8146(00)00205-3
  • Fowler, J. A. (1970). Control of vertebral number in teleosts-an embryological problem. The Quarterly Review of Biology, 45(2), 148-167.
  • Ganai, F. A., Wani, S., Ahmad, S., Yousuf, A. R., & Tripathi, N. K. (2014). Coupled biochemical genetic and karyomorphological analyses for taxonomic classification-A case study of Schizothorax species complex (Teleostei: Cyprinidae). African Journal of Biotechnology, 13(15), 1623-1630. https://doi.org/10.5897/AJB2013.13370
  • He, S., Mayden, R. L., Wang, X., Wang, W., Tang, K. L., Chen, W. J., & Chen, Y. (2008). Molecular phylogenetics of the family Cyprinidae (Actinopterygii: Cypriniformes) as evidenced by sequence variation in the first intron of S7 ribosomal protein-coding gene: Further evidence from a nuclear gene of the systematic chaos in the family. Molecular Phylogenetics and Evolution, 46(3), 818-829. https://doi.org/10.1016/j.ympev.2007.06.001
  • Hofmann, K. (1987). Fundamental problems in identifying the animal species of muscle meat using electrophoretic methods. Fleischwirtsch, 67, 820-828.
  • Hua, C. J., Zhang, D., Zou, H. Li, M., Jakovlić, I., Wu, S. G., Wang, G. T., & Li, W. X. (2019). Morphology is not a reliable taxonomic tool for the genus Lernaea: Molecular data and experimental infection reveal that L. cyprinacea and L. cruciata are conspecific. Parasites & Vectors, 12, 579. https://doi.org/10.1186/s13071-019-3831-y
  • Ihuţ, A., Răducu, C., Cocan, D., Munteanu, C., Luca, I., Uiuiu, P., Mireşan, V., Lațiu, C., & Rus, V. (2020) Seasonal variation of blood biomarkers in huchen, Hucho hucho (Actinopterygii: Salmoniformes: Salmonidae) reared in captivity. Acta Ichthyologica et Piscatoria, 50(4), 381-390. https://doi.org/10.3750/AIEP/02836
  • Imoto, J. M., Saitoh, K., Sasaki, T., Yonezawa, T., Adachi, J., Kartavtsev, Y. P., Miya M., Nishida, M., & Hanzawa, N. (2013). Phylogeny and biogeography of highly diverged freshwater fish species (Leuciscinae, Cyprinidae, Teleostei) inferred from mitochondrial genome analysis. Gene, 514(2), 112-124. https://doi.org/10.1016/j.gene.2012.10.019
  • Kuru, M. (2004). Türkiye iç su balıklarının son sistematik durumu [Recent systematic status of inland water fishes of Turkey]. Gazi Üniversitesi Gazi Eğitim Fakültesi Dergisi, 24(3), 1-21.
  • Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of Bacteriophage T4. Nature, 277(5259), 680-684. https://doi.org/10.1038/227680a0
  • Li, J., Wang, X., Kong, X., Zhao, K., He, S., & Mayden, R. L. (2008). Variation patterns of the mitochondrial 16S rRNA gene with secondary structure constraints and their application to phylogeny of cyprinine fishes (Teleostei: Cypriniformes). Molecular Phylogenetics and Evolution, 47(2), 472-487. https://doi.org/10.1016/j.ympev.2007.09.012
  • Martinez, I., Jakobsen, F. T., & Seppola, M. (2001). Requirements for the application of protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation. Electrophoresis, 22(8), 1526-1533. https://doi.org/10.1002/1522-2683(200105)22:8%3C1526::aid-elps1526%3E3.0.co;2-3
  • Muhammad, O. I., Mahmoud, U. M., Fazio, F., & Sayed, A. E. H. (2018). SDS-PAGE technique as biomarker for fish toxicological studies. Toxicology Reports, 5, 905–909. https://doi.org/10.1016/j.toxrep.2018.08.020
  • Nei, M. (1978). Estimation of average heterozygosity and genetic distances from a small number of individuals. Genetics, 89(3), 583-590. https://doi.org/10.1093/genetics/89.3.583
  • Nelson, J. S. (1994). Fishes of the World. John Wiley and Sons, Inc.
  • Nirenberg, N. W., Matthaei, J. H., Jones, O. W., Martin, R. G., & Barondes, S. H. (1963). Approximation of genetic code via cell free protein synthesis detected by template RNA. Federation Proceedings, 22, 55-61.
  • Ochoa, S. (1963). Synthetic polynucleotides and genetic code. Federation Proceedings, 22, 62-74.
  • Şalcıoğlu, A., Gubili, C., Krey, G., Sönmez, A. Y., & Bilgin, R. (2020). Phylogeography and population dynamics of the Eastern Mediterranean whiting (Merlangius merlangus) from the Black Sea, the Turkish Straits System, and the North Aegean Sea. Fisheries Research, 229, 105614. https://doi.org/10.1016/j.fishres.2020.105614
  • Tang, K. L., Agnew, M. K., Chen, W. J., Hirt, M. V., Raley, M. E., Sado, T., Schneider, L. M., Yang, L., Bart, H. L., He, S., Liu, H., Miya, M., Saitoh, K., Simons, A. M., Wooda, R. M., & Mayden, R. L. (2011). Phylogeny of the gudgeons (Teleostei: Cyprinidae: Gobioninae). Molecular Phylogenetics and Evolution, 61(1), 103-124. https://doi.org/10.1016/j.ympev.2011.05.022
  • Tang, K. L., Agnew, M. K., Hirt, M. V., Sado, T., Schneider, L. M., Freyhof, J., Sulaiman, Z., Swartz, E., Vidthayanon, C., Miya, M., Saitoh, K., Simons, A. M., Wood, R. M., & Mayden, R. L. (2010). Systematics of the subfamily Danioninae (Teleostei: Cypriniformes: Cyprinidae). Molecular Phylogenetics and Evolution, 57(1), 189-214. https://doi.org/10.1016/j.ympev.2010.05.021
  • Theophilus, J., & Rao, P. R. (1998). Electrophoretic studies on the serum proteins of the three species of genus Channa. Indian Journal of Fisheries, 35(4), 294-297.
  • Tokur, B., & Kandemir, S. (2008). Dondurulmuş balıklarda farklı çözündürme şekillerinin protein kalitesine olan etkileri [The effects of different thawing methods on protein quality of frozen fish]. Journal of FisheriesSciences.com, 2(1), 100-106. https://doi.org/10.3153/jfscom.2008010
  • Yılmaz, M., Çiğremiş, Y., Türköz, Y., & Gaffaroğlu, M. (2005). A taxonomic study on Orthrias insignis euphraticus (Banarescu and Nalbant, 1964) and Cyprinion macrostomus (Heckel, 1843) by sarcoplasmic protein electrophoresis. Gazi University Journal of Science, 18(1), 61-68.
  • Yılmaz, M., Türköz, Y., Erdemli, A. Ü., Kalkan, E., & Çiğremiş, Y. (2000). A taxonomical study on electrophoretic pattern of blood serum protein of some fish of Karakaya Dam Lake. Eurasian Journal of Veterinary Sciences, 16(1), 89-92.
  • Yilmaz, M., Yilmaz, H. R., & Alas, A. (2007). An electrophoretic taxonomic study on serum proteins of Acanthobrama marmid, Leuciscus cephalus, and Chondrostoma regium. EurAsian Journal of BioSciences, 3, 22-27.
There are 29 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Article
Authors

Emine Arslan 0000-0002-0782-506X

Elif Gülbahçe Mutlu 0000-0003-2391-2152

Project Number 14401063
Publication Date June 30, 2023
Submission Date April 12, 2023
Acceptance Date June 8, 2023
Published in Issue Year 2023 Volume: 12 Issue: 2

Cite

APA Arslan, E., & Gülbahçe Mutlu, E. (2023). Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa. Marine Science and Technology Bulletin, 12(2), 244-251. https://doi.org/10.33714/masteb.1281335
AMA Arslan E, Gülbahçe Mutlu E. Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa. Mar. Sci. Tech. Bull. June 2023;12(2):244-251. doi:10.33714/masteb.1281335
Chicago Arslan, Emine, and Elif Gülbahçe Mutlu. “Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa”. Marine Science and Technology Bulletin 12, no. 2 (June 2023): 244-51. https://doi.org/10.33714/masteb.1281335.
EndNote Arslan E, Gülbahçe Mutlu E (June 1, 2023) Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa. Marine Science and Technology Bulletin 12 2 244–251.
IEEE E. Arslan and E. Gülbahçe Mutlu, “Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa”, Mar. Sci. Tech. Bull., vol. 12, no. 2, pp. 244–251, 2023, doi: 10.33714/masteb.1281335.
ISNAD Arslan, Emine - Gülbahçe Mutlu, Elif. “Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa”. Marine Science and Technology Bulletin 12/2 (June 2023), 244-251. https://doi.org/10.33714/masteb.1281335.
JAMA Arslan E, Gülbahçe Mutlu E. Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa. Mar. Sci. Tech. Bull. 2023;12:244–251.
MLA Arslan, Emine and Elif Gülbahçe Mutlu. “Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa”. Marine Science and Technology Bulletin, vol. 12, no. 2, 2023, pp. 244-51, doi:10.33714/masteb.1281335.
Vancouver Arslan E, Gülbahçe Mutlu E. Investigation of Taxonomic Relationship and Effect of Seasonal Temperature Changes Based on Protein Profiles of Fishes From Beyşehir, Suğla Lakes and Dam Apa. Mar. Sci. Tech. Bull. 2023;12(2):244-51.
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