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Asetamiprid ve d-Tübokürarin'in Kurbağa Sinir Dokusu Üzerine Etkilerinin İncelenmesi (I: Oksidatif Potansiyel)

Year 2017, Volume: 21 Issue: 1, 149 - 155, 15.08.2016
https://doi.org/10.12991/marupj.259892

Abstract

Bu çalışmada, asetamiprid ve
d-tübokürarin’in Rana ridibunda  siyatik
siniri üzerine oksidatif stres etkisinin incelenmesi  amaçlanmıştır. Bu amaçla kurulan deneylerde,
40 adet kurbağa  siyatik sinir preparatı
kullanılmıştır. Kurbağalar, spinal hale  getirildikten
sonra siyatik sinirler izole edilmiştir. Sinirlere,  120 dakika boyunca 1×10-3, 1×10-4, 1×10-5 ve
1×10-6 M olmak  üzere dört farklı
konsantrasyondaki asetamiprid çözeltileri 
uygulanmıştır. Bunun yanı sıra siyatik sinir dokuları, 1x10-3  M asetamiprid ile 1x10-2 M d-tübokürarin
karışımı, 1x10-5  M asetamiprid ile
1x10-4 M d-tübokürarin karışımı ve 1x10-6 
M asetamiprid ile 1x10-5 M d-tübokürarin karışımında 120  dakika bekletilmiştir. Kontrol grubundaki
sinir dokuları, 120  dakika süresince
Ringer çözeltisinde bekletilmiştir. Tüm deney 
gruplarında agonist ve antagonist etkileri eşit sayıda denek  üzerinde çalışılmıştır (N=5). Kurbağalarda
oksidatif stresi  değerlendirmek için,
katalaz (CAT) ve asetilkolinesteraz (AChE) 
aktiviteleri ile malondialdehit (MDA) düzeyleri ölçülmüştür. 

Yapılan biyomarkır analizleri
sonucunda, 1x10-3 M  asetamiprid’in
siyatik sinirde, biyokimyasal parametrelerden 
CAT ve AChE aktivitesini kontrol grubuna göre önemli  biçimde azalttığı gözlenmiştir (P<0.05).
Buna karşılık aynı  konsantrasyondaki
insektisitin, sinir dokuda MDA seviyesini 
kontrol grubuna göre önemli biçimde arttırdığı tespit edilmiştir  (p<0.05). 





Asetamiprid’in siyatik sinir
dokusunda, biyokimyasal  parametrelerden
CAT ile AChE’yi anlamlı şekilde düşürmesi, 
MDA’yı ise önemli biçimde yükseltmesi, bu insektisitin yüksek  konsantrasyonda periferik sinirler üzerine
oksidatif strese bağlı  hasar meydana
getirdiğini göstermektedir.  

References

  • KAYNAKLAR
  • Vural N. Toksikoloji. Ankara Üniversitesi Eczacılık Fakültesi Yayınları, No: 73, Ankara Üniversitesi Basımevi, Ankara, 2005; pp. 334-377.
  • Matsuda K, Buckingham SD, Kleier D, Rauh JJ, Grauso M, Sattelle DB. Neonicotinoids: İnsecticides acting on insect nicotinic acetylcholine receptors. Trends Pharmacol Sci 2001; 22(11): 573-580.
  • Soloway SB, Hanry AC, Kollmeyer WD, Padgett WM, Powell JE, Roman SA, Tieman CH, Corey RA, Horne CA. Nitromethylene Insecticides, Advances in Pesticide Science. Editors: Geissbühler H, Kearney PC and Brooks GT, Pergamon Press, Zurich, 1979; pp. 206-217.
  • Genper JI, Hall LM, Sattelle DB. Insect acetylcholine receptors as a site of insecticide action. Nature 1978; 276: 188-190.
  • Farooqui T. A potential link among biogenic amines-based pesticides, learning and memory and colony collapse disorder: A unique hypothesis. Neurochem Int 2013; 62: 122-136.
  • Nauen R, Kintscher UE, Salgado VL, Kaussmann M. Thiamethoxam is a neonicotinoid precursor converted to clothianidin in insects and plants. Pest Biochem Physiol 2003; 76: 55-69.
  • Scharf ME. Neurological effects of insecticides, Encyclopedia of Pest Management. Editor: Pimental D. Marcel-Dekker, New York, 2003, pp. 1-5.
  • Klaunig JE. Alterations in Intracellular Communication During the Stage of Promotion. Exp Biol Med 1991; 198(2): 688-692.
  • Scassellati SG, Moretti M, Villarini M, Angeli G, Pasquini R, Monarca S, Scarselli R, Crea MG, Lonardis C. An Evaluation of Toxic and Genotoxic Risk From Work Related Exposure to Chemical Compounds. Prev Oggi 1994; 6: 125-138.
  • Banerjee BD, Seth V, Bhattacharya A, Pahsa ST, Chakraborty AK. Biochemical Effects of Some Pesticides on Lipid Peroxidation and Free-Radical Scavengers. Toxicol Lett 1999; 107: 33-47.
  • Aruoma OI. Free Radicals, Oxidative Stress, and Antioxidants in Human Health and Disease. J Am Oil Chem Soc 1998; 75: 199-212.
  • John S, Kale M, Rathore N, Bhatnagar D. Protective Effect of Vitamin E in Dimethoate and Malathion Induced Oxidative Stress in Rat Erythrocytes. J Nutr Biochem 2001; 12: 500-504.
  • Keramati V, Jamili S, Ramin M. Effect of Diazinon on Catalase Antioxidant Enzyme Activity in Liver Tissue of Rutilus rutilus. J Fish Aquat Sci 2010; 5: 368-376.
  • Yu M, Li SM, Li XY, Zhang BJ, Wang JJ. Acute Effects of 1-Octyl-3-Methylimidazolium Bromide Ionic Liquid on the Antioxidant Enzyme System of Mouse Liver. Ecotoxicol Environ Saf 2008; 71: 903-908.
  • Özeti N, Yılmaz İ. Türkiye Amfibileri, Ege Üniversitesi Basımevi, İzmir, 1994; pp. 141-159.
  • Demirsoy A. Türkiye Omurgalıları-Amfibileri, Meteksan A.Ş., Ankara, 1996; pp. 69-73.
  • Pehlivan F. Biyofizik, Hacettepe T.A.Ş., Ankara, 1997; pp. 167-183.
  • Aebi H. Catalase in vitro, Methods Enzymol 1984; 105: 121-126.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-358.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein Measurement with the Folin Phenol Reagent. J Biol Chem 1961; 193: 265-275.
  • Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharm 1961; 7: 88-95.
  • Kumargal D, Çömelekoğlu Ü, Aşkın A. İyon kanallarını hedef alan insektisitler, Mersin Üniv Saglık Bilim Derg 2012; 5(2): 7-13.
  • Eddleston M, Bateman DN. Specific substances, Pesticides. Medicine 2012; 40(3): 147-150.
  • Aliferis KA, Jabaji S. Metabolomics - a robust bioanalytical approach for the discovery of the modes-of-action of pesticides: A review. Pest Biochem Physiol 2011; 100: 105-117.
  • Tan J, Galligan JJ, Hollingworth RM. Agonist actions of nicotinoids on nicotinic acetylcholine receptors expressed by cockroach neurons. Neurotoxicology 2007; 829-842.
  • Thany SH. Agonist actions of clothianidin on synaptic and extrasynaptic nicotinic acetylcholine receptors expressed on cockroach sixth abdominal ganglion, Neurotoxicology 2009; 1045-1052.
  • Ballı E, Yalın S, Mazmancı B, Mazmancı MA, Söğüt F, Eroğlu P, Yetkin D, Korkutan S, Çömelekoğlu Ü. Deltametrinin oluşturduğu periferik sinir hasarları üzerine E vitaminin etkisinin araştırılması. Mersin Üniv Saglık Bilim Derg 2014; 7(1): 17-23.
  • Acker CI, Souza ACG, Pinton S, Rocha JT, Friggi CA, Zanella R, Nogueira CW. Repeated malathion exposure induces behavioral impairment and AChE activity inhibition in brains of rat pups. Ecotoxicol Environ Saf 2011; 74: 2310-2315.
  • Beneteau AB, Carvalho SM, Brunet JL, Carvalho GA, Bulete A, Giroud B, Belzunces LP. Development of biomarkers of exposure to xenobiotics in the honey bee Apis mellifera: Application to the systemic insecticide thiamethoxam. Ecotoxicol Environ Saf 2012; 82: 22-31.
  • Bloomquist JR. Ion channels as targets for insecticides. Ann Rev Entomol 1996; 41: 163-190.
  • Casida JE, Quistad GB. Golden age of insecticide research: Past, present and future. Ann Rev Entomol 1998; 43: 1-16.
  • Düzgüner V, Erdoğan S. Acute Oxidant and Inflammatory Effects of Imidacloprid on the Mammalian Central Nervous System and Liver in Rats. Pest Biochem Physiol 2010; 97(1): 13-18.
  • Thapar A, Sandhir R, Kiran R. Acephate Induced Oxidative Stress in Erythrocytes. Indian J Exp Biol 2002; 40(8): 963-966.
  • Singh M, Sandhir R, Kiran R. Erythrocyte Antioxidant Enzymes in Toxicological Evaluation of Commonly Used Organophasphate Pesticides. Indian J Exp Biol 2006; 44(7): 580-583.
  • Cheeseman KH. Mechanisms and Effects of Lipid Peroxidation. Molecular Aspects of Medicine 1993; 14(3): 191-197.
  • Kanbur M, Liman BC, Eraslan G, Altınordulu S. Effects of Cypermethrin, Propetamphos, and Combination Involving Cypermethrin and Propetamphos on Lipid Peroxidation in Mice. Environ Toxicol 2008; 23(4): 473-479.
  • Düzgüner V, Erdoğan S. Chronic Exposure to Imidacloprid Induces Inflammation and Oxidative Stress in the Liver and Central Nervous System of Rats. Pest Biochem Physiol 2012; 104(1): 58-64.
  • Clasen B, Leitemperger J, Murussi C, Pretto A, Menezes C, Dalabona F, Marchezan E, Adaime MB, Zanella R, Loro VL. Carbofuran Promotes Biochemical Changes in Carp Exposed to Rice Feld and Laboratory Conditions. Ecotoxicol Environ Saf 2014; 101: 77-82.
  • MacMahon B. Pesticide residues and breast cancer. J Natl Cancer Inst 1994; 86: 572-573.

Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential)

Year 2017, Volume: 21 Issue: 1, 149 - 155, 15.08.2016
https://doi.org/10.12991/marupj.259892

Abstract

The aim of this study is to
investigate the oxidative stress effect  of
acetamiprid and d-tubocurarine on the sciatic nerve of Rana  ridibunda by using biochemical methods. For
this purpose, 40  frog sciatic nerve
preparations were used in the experiments 
established. Frog sciatic nerves were isolated after making  them spinal. Four different concentrations of
acetamiprid  solution (1×10-3, 1×10-4,
1×10-5 and 1×10-6 M) were applied on  nerves
for 120 minutes. In addition, the sciatic nerve tissues  were maintained for 120 minutes in the
mixture of 1x10-3 M  acetamiprid and
1x10-2 M d-tubocurarine, the mixture of 1x10-5 M acetamiprid and 1x10-4 M d-tubocurarine, the mixture of  1x10-6 M acetamiprid and 1x10-5 M
d-tubocurarine. Nerve  tissues in the
control group were maintained in Ringer’s solution  for 120 minutes. The agonist and antagonist
effects were studied  in the experimental
group and equal number of subjects (N =  5).
Activity of catalase (CAT) and acetylcholinesterase (AChE)  as well as malondialdehyde (MDA) levels were
measured to  evaluate oxidative stress in
frogs. 

The results of the biomarker
analysis revealed that the  application
of 1x10-3 M acetamiprid on sciatic nerve significantly  reduced the CAT and AChE activity compared to
that of control  group (p<0.05). In
contrast, it was determined that, the same 
concentration of the insecticide significantly increased the level  of MDA on nerve tissue compared to that of
control group  (p<0.05). 





The significant reduction of CAT
and AChE and significant  increase of MDA
in the sciatic nerve due to acetamiprid indicate  that this insecticide, depending upon the
oxidative stress, causes  damage on
peripheral nerves in high concentrations.  

References

  • KAYNAKLAR
  • Vural N. Toksikoloji. Ankara Üniversitesi Eczacılık Fakültesi Yayınları, No: 73, Ankara Üniversitesi Basımevi, Ankara, 2005; pp. 334-377.
  • Matsuda K, Buckingham SD, Kleier D, Rauh JJ, Grauso M, Sattelle DB. Neonicotinoids: İnsecticides acting on insect nicotinic acetylcholine receptors. Trends Pharmacol Sci 2001; 22(11): 573-580.
  • Soloway SB, Hanry AC, Kollmeyer WD, Padgett WM, Powell JE, Roman SA, Tieman CH, Corey RA, Horne CA. Nitromethylene Insecticides, Advances in Pesticide Science. Editors: Geissbühler H, Kearney PC and Brooks GT, Pergamon Press, Zurich, 1979; pp. 206-217.
  • Genper JI, Hall LM, Sattelle DB. Insect acetylcholine receptors as a site of insecticide action. Nature 1978; 276: 188-190.
  • Farooqui T. A potential link among biogenic amines-based pesticides, learning and memory and colony collapse disorder: A unique hypothesis. Neurochem Int 2013; 62: 122-136.
  • Nauen R, Kintscher UE, Salgado VL, Kaussmann M. Thiamethoxam is a neonicotinoid precursor converted to clothianidin in insects and plants. Pest Biochem Physiol 2003; 76: 55-69.
  • Scharf ME. Neurological effects of insecticides, Encyclopedia of Pest Management. Editor: Pimental D. Marcel-Dekker, New York, 2003, pp. 1-5.
  • Klaunig JE. Alterations in Intracellular Communication During the Stage of Promotion. Exp Biol Med 1991; 198(2): 688-692.
  • Scassellati SG, Moretti M, Villarini M, Angeli G, Pasquini R, Monarca S, Scarselli R, Crea MG, Lonardis C. An Evaluation of Toxic and Genotoxic Risk From Work Related Exposure to Chemical Compounds. Prev Oggi 1994; 6: 125-138.
  • Banerjee BD, Seth V, Bhattacharya A, Pahsa ST, Chakraborty AK. Biochemical Effects of Some Pesticides on Lipid Peroxidation and Free-Radical Scavengers. Toxicol Lett 1999; 107: 33-47.
  • Aruoma OI. Free Radicals, Oxidative Stress, and Antioxidants in Human Health and Disease. J Am Oil Chem Soc 1998; 75: 199-212.
  • John S, Kale M, Rathore N, Bhatnagar D. Protective Effect of Vitamin E in Dimethoate and Malathion Induced Oxidative Stress in Rat Erythrocytes. J Nutr Biochem 2001; 12: 500-504.
  • Keramati V, Jamili S, Ramin M. Effect of Diazinon on Catalase Antioxidant Enzyme Activity in Liver Tissue of Rutilus rutilus. J Fish Aquat Sci 2010; 5: 368-376.
  • Yu M, Li SM, Li XY, Zhang BJ, Wang JJ. Acute Effects of 1-Octyl-3-Methylimidazolium Bromide Ionic Liquid on the Antioxidant Enzyme System of Mouse Liver. Ecotoxicol Environ Saf 2008; 71: 903-908.
  • Özeti N, Yılmaz İ. Türkiye Amfibileri, Ege Üniversitesi Basımevi, İzmir, 1994; pp. 141-159.
  • Demirsoy A. Türkiye Omurgalıları-Amfibileri, Meteksan A.Ş., Ankara, 1996; pp. 69-73.
  • Pehlivan F. Biyofizik, Hacettepe T.A.Ş., Ankara, 1997; pp. 167-183.
  • Aebi H. Catalase in vitro, Methods Enzymol 1984; 105: 121-126.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-358.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein Measurement with the Folin Phenol Reagent. J Biol Chem 1961; 193: 265-275.
  • Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharm 1961; 7: 88-95.
  • Kumargal D, Çömelekoğlu Ü, Aşkın A. İyon kanallarını hedef alan insektisitler, Mersin Üniv Saglık Bilim Derg 2012; 5(2): 7-13.
  • Eddleston M, Bateman DN. Specific substances, Pesticides. Medicine 2012; 40(3): 147-150.
  • Aliferis KA, Jabaji S. Metabolomics - a robust bioanalytical approach for the discovery of the modes-of-action of pesticides: A review. Pest Biochem Physiol 2011; 100: 105-117.
  • Tan J, Galligan JJ, Hollingworth RM. Agonist actions of nicotinoids on nicotinic acetylcholine receptors expressed by cockroach neurons. Neurotoxicology 2007; 829-842.
  • Thany SH. Agonist actions of clothianidin on synaptic and extrasynaptic nicotinic acetylcholine receptors expressed on cockroach sixth abdominal ganglion, Neurotoxicology 2009; 1045-1052.
  • Ballı E, Yalın S, Mazmancı B, Mazmancı MA, Söğüt F, Eroğlu P, Yetkin D, Korkutan S, Çömelekoğlu Ü. Deltametrinin oluşturduğu periferik sinir hasarları üzerine E vitaminin etkisinin araştırılması. Mersin Üniv Saglık Bilim Derg 2014; 7(1): 17-23.
  • Acker CI, Souza ACG, Pinton S, Rocha JT, Friggi CA, Zanella R, Nogueira CW. Repeated malathion exposure induces behavioral impairment and AChE activity inhibition in brains of rat pups. Ecotoxicol Environ Saf 2011; 74: 2310-2315.
  • Beneteau AB, Carvalho SM, Brunet JL, Carvalho GA, Bulete A, Giroud B, Belzunces LP. Development of biomarkers of exposure to xenobiotics in the honey bee Apis mellifera: Application to the systemic insecticide thiamethoxam. Ecotoxicol Environ Saf 2012; 82: 22-31.
  • Bloomquist JR. Ion channels as targets for insecticides. Ann Rev Entomol 1996; 41: 163-190.
  • Casida JE, Quistad GB. Golden age of insecticide research: Past, present and future. Ann Rev Entomol 1998; 43: 1-16.
  • Düzgüner V, Erdoğan S. Acute Oxidant and Inflammatory Effects of Imidacloprid on the Mammalian Central Nervous System and Liver in Rats. Pest Biochem Physiol 2010; 97(1): 13-18.
  • Thapar A, Sandhir R, Kiran R. Acephate Induced Oxidative Stress in Erythrocytes. Indian J Exp Biol 2002; 40(8): 963-966.
  • Singh M, Sandhir R, Kiran R. Erythrocyte Antioxidant Enzymes in Toxicological Evaluation of Commonly Used Organophasphate Pesticides. Indian J Exp Biol 2006; 44(7): 580-583.
  • Cheeseman KH. Mechanisms and Effects of Lipid Peroxidation. Molecular Aspects of Medicine 1993; 14(3): 191-197.
  • Kanbur M, Liman BC, Eraslan G, Altınordulu S. Effects of Cypermethrin, Propetamphos, and Combination Involving Cypermethrin and Propetamphos on Lipid Peroxidation in Mice. Environ Toxicol 2008; 23(4): 473-479.
  • Düzgüner V, Erdoğan S. Chronic Exposure to Imidacloprid Induces Inflammation and Oxidative Stress in the Liver and Central Nervous System of Rats. Pest Biochem Physiol 2012; 104(1): 58-64.
  • Clasen B, Leitemperger J, Murussi C, Pretto A, Menezes C, Dalabona F, Marchezan E, Adaime MB, Zanella R, Loro VL. Carbofuran Promotes Biochemical Changes in Carp Exposed to Rice Feld and Laboratory Conditions. Ecotoxicol Environ Saf 2014; 101: 77-82.
  • MacMahon B. Pesticide residues and breast cancer. J Natl Cancer Inst 1994; 86: 572-573.
There are 40 citations in total.

Details

Subjects Health Care Administration
Journal Section Articles
Authors

Yusuf Çamlıca

Salih Cüfer Bediz This is me

Serap Yalın

Publication Date August 15, 2016
Published in Issue Year 2017 Volume: 21 Issue: 1

Cite

APA Çamlıca, Y., Bediz, S. C., & Yalın, S. (2016). Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential). Marmara Pharmaceutical Journal, 21(1), 149-155. https://doi.org/10.12991/marupj.259892
AMA Çamlıca Y, Bediz SC, Yalın S. Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential). Marmara Pharm J. September 2016;21(1):149-155. doi:10.12991/marupj.259892
Chicago Çamlıca, Yusuf, Salih Cüfer Bediz, and Serap Yalın. “Investigation of Effect of Acetamiprid and D-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential)”. Marmara Pharmaceutical Journal 21, no. 1 (September 2016): 149-55. https://doi.org/10.12991/marupj.259892.
EndNote Çamlıca Y, Bediz SC, Yalın S (September 1, 2016) Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential). Marmara Pharmaceutical Journal 21 1 149–155.
IEEE Y. Çamlıca, S. C. Bediz, and S. Yalın, “Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential)”, Marmara Pharm J, vol. 21, no. 1, pp. 149–155, 2016, doi: 10.12991/marupj.259892.
ISNAD Çamlıca, Yusuf et al. “Investigation of Effect of Acetamiprid and D-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential)”. Marmara Pharmaceutical Journal 21/1 (September 2016), 149-155. https://doi.org/10.12991/marupj.259892.
JAMA Çamlıca Y, Bediz SC, Yalın S. Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential). Marmara Pharm J. 2016;21:149–155.
MLA Çamlıca, Yusuf et al. “Investigation of Effect of Acetamiprid and D-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential)”. Marmara Pharmaceutical Journal, vol. 21, no. 1, 2016, pp. 149-55, doi:10.12991/marupj.259892.
Vancouver Çamlıca Y, Bediz SC, Yalın S. Investigation of Effect of Acetamiprid and d-Tubocurarine on Frog Nerve Tissue (I: Oxidative Potential). Marmara Pharm J. 2016;21(1):149-55.