Antioxidant properties and enzyme inhibitory effects of extracts from Mandragora autumnalis and its fatty acid composition
Abstract
The present research was undertaken to evaluate antioxidant activities and enzyme inhibitory potentials of two extracts from two parts of Mandragora autumnalis. The antioxidant properties were evaluated by using different methods [ABTS (2,2-azino-bis (3-ethylbenzothiazloine-6-sulphonic acid)), DPPH (1,1 diphenyl-2-picrylhydrazyl radical), CUPRAC (cupric ion reducing antioxidant capacity), and FRAP (ferric reducing antioxidant power assays)]. Enzyme inhibitory potentials were tested against cholinesterase (AChE and BChE), tyrosinase, α-amylase and α-glucosidase. For phytochemical composition, fatty acids, total phenolics and flavonoid content were investigated. Generally F-Met exhibited the strongest antioxidant effect with the highest level of phenolics. Total phenolic and flavonoid content were determined as 26.10-46.92 mgGAE/g abd 3.60-26.11 mgRE/g, respectively. α- linolenic acid was found to be major fatty acids in the studied oils (33.81 % for flowers and 39.15 % for leaves). The study suggested that M. autumnalis could be valuable as a source of natural agents in food and pharmaceutical applications.
References
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- Alpinar K, Ozyurek M, Kolak U, Guclu K, Aras C, Altun M, Celik SE, Berker KI, Bektasoglu B, Apak R: Antioxidant Capacities of Some Food Plants Wildly Grown in Ayvalik of Turkey. Food Sci Tech Res 2009; 15: 59-64.
- Bors W, Werner H, Michel C, Saran M. Flavonoids as antioxidants: Determination of radical-scavenging efficiencies. Methods Enzymol 1990; 186: 343–355.
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- Zengin G, Uysal S, Ceylan R, Aktumsek A. Phenolic constituent, antioxidative and tyrosinase inhibitory activity of Ornithogalum narbonense L. from Turkey: A phytochemical study. Ind Crop Prod 2015; 70: 1-6.
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- Nagulsamy P, Ponnusamy R, Thangaraj P. Evaluation of antioxidant, anti-inflammatory, and antiulcer properties of Vaccinum leschenaultia Wight: A therapeutic supplement. J Food Drug Anal 2005, 23:376-386.
- Koleva, II, Van Beek JPH, Linseen A, De G, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem. Anal. 2002; 10: 178–182.
- Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem 2005; 53: 1841–1856.
- Prasad KN, Yang B, Shi J, Yu C, Zhao M, Xue S, Jiang Y. Enhanced antioxidant and antityrosinase activities of longan fruit pericarp by ultra-high-pressure-assisted extraction. J Pharm Biomed Anal 2010; 51: 471–47.
- Fatiha B, Didier H, Naima G, Khodir M, Martin K, Léocadie K, Caroline S, Mohamed C, Pierre D. Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae). Ind Crop Prod 2015; 74: 722-730.
Antioxidant properties and enzyme inhibitory effects of extracts from Mandragora autumnalis and its fatty acid composition
Abstract
References
- Novak J, Bitsch C, Langbehn J, Pank F, Skoula M, Gotsiou Y. Ratios Of cis-trans-sabinene hydrate in Origanum majorana L. and Origanum microphyllum. Biochem. Syst. Ecol. 2000; 28: 697–704.
- Guner A, Aslan S, Ekim T, Vural M, Babac MT. (edlr.) Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırma Derneği Yayını. İstanbul. 2012.
- Baytop T. Türkçe Bitki Adları Sözlüğü. Türk Dil Kurumu Yayınları 2007.
- Ramoutsaki IA, Dimitriou H, Kalmanti M. Management of childhood diseases in the Byzantine period: I—Analgesia. Pediatrics Int 2002; 44: 335–337.
- Chidiac EJ, Romeo MD, Kaddoum MD, Samir F, Fuleihan MD. Mandragora : Anesthetic of the Ancients. Anesth Analg 2012; 115: 1437-1441.
- Zohary M. Plants of the Bible. Cambridge University Press, Cambridge. 1982.
- Bekkouche K, Lazrek HB, Jana M. Plant. Med Phytother 1993; 26: 309.
- Nutton V. The rise of medicine, in: R. Porter (Ed.), Cambridge Illustrated History of Medicine, Cambridge Univ. Press, Cambridge. 1996; 52–81.
- Von Hintzenstern U. Anaesthesia with mandrake in the tradition of Dioskorides and its role in classical antiquity, in: R.S. Atkinson, T.B. Boulton (Eds.), The History of Anaesthesia, Royal Society of Medicine International Congress and Symposium Series, The Parthenon Publishing Group, London 1989; 134: 38–40.
- Al-Khalil S, Alkofahi A. The chemical constituents of Mandragora autumnalis. Alex J Pharm Sci 1996; 10: 135-138.
- Alzheimer’s Disease International, World Alzheimer Report. Alzheimer’s Disease International 2009; 1–24.
- International Diabetes Atlas, Seven Edition. 2015.
- Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Vitic 1977; 28: 49–55.
- Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardization of a propolis extract and identification of the main constituents. J Pharm de Belg 1994; 49: 462-468.
- Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 1999; 26: 1231–1237.
- Berk S, Tepe B, Arslan S, Sarikurkcu C. Screening of the antioxidant, antimicrobial and DNA damage protection potentials of the aqueous extract of Asplenium ceterach D.C. Afr J Biotechnol 2011; 10: 8902-8908.
- Apak R, Guclu K, Ozyurek M, Karademir SE, Ercag E. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. Int J Food Sci Nutr 2006; 57: 292-304.
- Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Antioxidant potentials and antisholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food Chem Toxicol 2013; 55: 290-296.
- Zengin G, Aktumsek A. Investigation of antioxidant potentials of solvent extracts from different anatomical parts of Asphodeline anatolica E. Tuzlaci: an endemic plant to Turkey. Afr J Tradit Complem 2014; 11: 481-488.
- Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7: 88–90.
- Orhan IE, Senol FS, Gulpinar AR, Sekeroglu N, Kartal M, Sener B. Neuroprotective potential of some terebinth coffee brands and the unprocessed fruits of Pistica terebinthus L: and their fatty and essential oil analyses. Food Chem 2012; 130: 882-888.
- Yang XW, Huang MZ, Jin YS, Sun LN, Song Y, Chen HS. Phenolics from Bidens bipinnata and their amylase inhibitory properties. Fitoterapia 2012; 83: 1169-1175.
- Palanisamy UD, Ling LT, Manaharan T, Appleton D. Rapid isolation of geraniin from Nephelium lappaceum rind waste and its anti-hyperglycemic activity. Food Chem 2011; 127: 21-27.
- Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Assessment of the antioxidant potential and fatty acid compositionof four Centaurea L. taxa from Turkey. Food Chem 2013; 141: 91-97.
- Kliebenstein DJ. Secondary metabolites and plant/environmentinteractions: A view through Arabidopsis thaliana tinged glasses. Plant Cell Environ 2004; 27: 675-684.
- Middleton E, Kandaswami C, Theoharides TC: The effects of plantflavonoids on mammalian cells: Implications for inflammation, heart disease and cancer. Pharmacol Rev 2000; 52: 673-751.
- Alpınar K, Ozyurek M, Kolak U, Guclu K, Aras C, Alpinar K, Altun ML, Celik SEK, Bektasoglu B, Berker KI, Apak R. Antioxidant Capacities of Some Food Plants Wildly Grown in Ayvalik of Turkey. Food Sci Tech Res 2009; 15: 59-64.
- Alpinar K, Ozyurek M, Kolak U, Guclu K, Aras C, Altun M, Celik SE, Berker KI, Bektasoglu B, Apak R: Antioxidant Capacities of Some Food Plants Wildly Grown in Ayvalik of Turkey. Food Sci Tech Res 2009; 15: 59-64.
- Bors W, Werner H, Michel C, Saran M. Flavonoids as antioxidants: Determination of radical-scavenging efficiencies. Methods Enzymol 1990; 186: 343–355.
- Hanasaki Y, Ogawa S, Fukui S. The correlation between active oxygen scavenging and antioxidative effects of flavonoids. Free Radic Biol Med 1994; 16: 845–850.
- Zengin G, Uysal S, Ceylan R, Aktumsek A. Phenolic constituent, antioxidative and tyrosinase inhibitory activity of Ornithogalum narbonense L. from Turkey: A phytochemical study. Ind Crop Prod 2015; 70: 1-6.
- Uysal S, Zengin G, Aktumsek A, Karatas S. Fatty acid composition, total sugar content and anti-diabetic activity of methanol and water extracts of nine different fruit tree leaves collected from Mediterranean Region of Turkey. Int J Food Prop 2015; 18: 2268-2276.
- Nagulsamy P, Ponnusamy R, Thangaraj P. Evaluation of antioxidant, anti-inflammatory, and antiulcer properties of Vaccinum leschenaultia Wight: A therapeutic supplement. J Food Drug Anal 2005, 23:376-386.
- Koleva, II, Van Beek JPH, Linseen A, De G, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem. Anal. 2002; 10: 178–182.
- Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem 2005; 53: 1841–1856.
- Prasad KN, Yang B, Shi J, Yu C, Zhao M, Xue S, Jiang Y. Enhanced antioxidant and antityrosinase activities of longan fruit pericarp by ultra-high-pressure-assisted extraction. J Pharm Biomed Anal 2010; 51: 471–47.
- Fatiha B, Didier H, Naima G, Khodir M, Martin K, Léocadie K, Caroline S, Mohamed C, Pierre D. Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae). Ind Crop Prod 2015; 74: 722-730.
There are 37 citations in total.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | January 27, 2016 |
| Published in Issue | Year 2016 Volume: 20 Issue: 2 |