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Salivary Thromboplastic Activity in Children with Cancer

Year 2013, Volume: 1 Issue: 2, 67 - 70, 19.12.2014

Abstract

Thromboplastin is a membrane bound glycoprotein and it is
overexpressed in many cancer cells. Chemotherapy induce some
alterations in either saliva composition or function leading to oral
complications. The aim of this study was to investigate the salivary
thromboplastic activity of children with cancer and to compare
with healthy children. Saliva samples were taken from 15 children
who were diagnosed with lymphoid and solid tumor and from 15
healthy children. In saliva samples thromboplastic activity and
total protein levels were determined and SDS polyacrylamide gel
electrophoresis was also carried out with these saliva samples. For
cytological examination, imprint samples were prepared by
applying a drop of saliva on a slide. Salivary thromboplastic
activity of children with cancer (CC) was significantly decreased
when compared with the healthy children (HC). pH of the saliva in
CC was significantly decreased. Salivary flow rate did not
significantly change in CC. Saliva total protein level was
significantly decreased in CC when compared to HC. In SDS
PAGE electrophoresis, some protein bands decreased and some
protein bands increased when the two group is compared. In
cytologic evaluation of saliva samples, keratinized and dysplastic
cells increased in the imprint samples of CC. It should be noted
that children receiving chemotherapy encounter major problems
in oral and dental health, therefore they need special preventive
programs for their oral and dental health.

References

  • Ünür M, Onur Ö: Ağız hastalıklarının teşhis ve tedavisi. İstanbul, Quintessence Yayıncılık Ltd.Sti. 2003, pp 86-87.
  • Dummett CO : Anomallies of the developing dentition, in: Pinkham JR (ed) Pediatric Dentistry. Philadelphia, WB. Saunders Co, 1999, p.60.
  • Zacharski LR, Rosenstein R : Reduction of salivary tissue factor (Thromboplastin) activity by warfarin therapy. Blood 1979; 53(3), 366-374.
  • Yarat A, Tunalı T, Pişiriciler R, Akyüz S, İpbüker A, Emekli N: Salivary thromboplastic activity in diabetics and healthy controls. Clin Oral Invest 2004; 8:36-39.
  • Tunali-Akbay T , Guvercin M, Gonul O, Yarat A, Akyuz S, Pisiriciler R, Göker K: Salivary Thromboplastic Activity May Indicate Wound Healing In Tooth Extraction. Balk J Stom 2010; 14:141 -144,
  • Yarat A, Akyuz S, Tunali T, Kuscu O, Pisiriciler R: Salivary tissue factor activity and dental caries in in 4-12 years old childeren. OHDMBSC. 2007; 1(2): 44 - 50.
  • Emekli Alturfan E, Demir G, Kasıkcı E, Tunali-Akbay T, Pisiriciler R, Calıskan E, Yarat A: Altered biochemical parameters in the saliva of patients with breast cancer. Tohoku J Exp Med. 2008; 214(2): 89-96.
  • Doku HC, Taylor RG: Thromboplastin generation by saliva. Oral Surg Oral Med Oral Pathol 1962;15:1295-1301.
  • Maiolo A, Tua A, Grignani G : Hemostasis and cancer: tumor cells induce the expression of tissue factor-like procoagulant activity on endothelial cells. Hemostasis 2002; 87: 624-628.
  • Roberts HR, Daugald MU, Hoffman M: Molecular Biology and Biochemistry of the Coagulant Factor and Pathways of Hemostasis, in Beutler E, Lichtman MA,Coller BS, Kipps TJ, Seligshon U (eds): Williams Hematology. Newyork, McGrawHill, 2001, pp 1409-1434.
  • Yarat A: Tromboplastik Aktivite (Doku faktörü aktivitesi) 4.Ulusal Tromboz,Hemostaz ve Anjioloji Kongresi 26-28 Eylül 2003- Trakya Üniversitesi Tıp Fakültesi, Edirne, s.97-105.
  • Jiang X, Konigshey UH, Brombey ME: Formation of tissue factor-Factor VIIa Complex promotor cellular signaling and migration of tumor breast cancer cells. J Thromb Haemost 2003; 1(9): 1972-1976.
  • Ingram GIC, Hills M : Reference method for the one-stage prothrombin-time test on human blood. Thromb Haemostas 1976; 36 : 237-238.
  • Lowry OH, Rosebrough WI, Farr A, Randal RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-275.
  • Laemmli UK Cleavage of structural proteins during the assembly of the head of bacteriofage T4. Nature 1970; 227: 680-685.
  • Atay Z, Topalidis T : Cytodiagnostik der Serösen Höhlen. Atlas und Lehrbuch. Hannover , Wolfgang Pabst verlag, 1994; 336.
  • Grignani G, Mailo A: Cytokines and hemostasis. Haematologica.2000; 73(2):145-150.
  • Hathcock J: Vascular biology. The role of tissue factor Hematol. 2004; 41(1): 30-34.
  • Berckmans RJ, Sturk A, van Tienen LM, Schaap MCL, Nieuwland R: Cell-derived vesicles exposing coagulant tissue factor in saliva. Blood 2011; 117(11): 31172-31180.
  • Thylstrup A, Federskov O.: Textbook of Clinical Cariology. Copenhagen, Munkgoard, 1986; pp 28-45.
  • Jenkins NG: The physiology and biochemistry of mouth. Oxford Blackwell Scientific Publications, 1978; pp284-360.
  • Brand HS, Veerman EC: Saliva and wound healing. Chin J Dent Res  2013;16(1):7-12.
  • Kleinjan A, Böing AN, Sturk A, Nieuwland R: Microparticles in vascular disorders: How tissue factor-exposing vesicles contribute to pathology and physiology. Thromb Res 2012; 130: 71–73.

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Year 2013, Volume: 1 Issue: 2, 67 - 70, 19.12.2014

Abstract

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References

  • Ünür M, Onur Ö: Ağız hastalıklarının teşhis ve tedavisi. İstanbul, Quintessence Yayıncılık Ltd.Sti. 2003, pp 86-87.
  • Dummett CO : Anomallies of the developing dentition, in: Pinkham JR (ed) Pediatric Dentistry. Philadelphia, WB. Saunders Co, 1999, p.60.
  • Zacharski LR, Rosenstein R : Reduction of salivary tissue factor (Thromboplastin) activity by warfarin therapy. Blood 1979; 53(3), 366-374.
  • Yarat A, Tunalı T, Pişiriciler R, Akyüz S, İpbüker A, Emekli N: Salivary thromboplastic activity in diabetics and healthy controls. Clin Oral Invest 2004; 8:36-39.
  • Tunali-Akbay T , Guvercin M, Gonul O, Yarat A, Akyuz S, Pisiriciler R, Göker K: Salivary Thromboplastic Activity May Indicate Wound Healing In Tooth Extraction. Balk J Stom 2010; 14:141 -144,
  • Yarat A, Akyuz S, Tunali T, Kuscu O, Pisiriciler R: Salivary tissue factor activity and dental caries in in 4-12 years old childeren. OHDMBSC. 2007; 1(2): 44 - 50.
  • Emekli Alturfan E, Demir G, Kasıkcı E, Tunali-Akbay T, Pisiriciler R, Calıskan E, Yarat A: Altered biochemical parameters in the saliva of patients with breast cancer. Tohoku J Exp Med. 2008; 214(2): 89-96.
  • Doku HC, Taylor RG: Thromboplastin generation by saliva. Oral Surg Oral Med Oral Pathol 1962;15:1295-1301.
  • Maiolo A, Tua A, Grignani G : Hemostasis and cancer: tumor cells induce the expression of tissue factor-like procoagulant activity on endothelial cells. Hemostasis 2002; 87: 624-628.
  • Roberts HR, Daugald MU, Hoffman M: Molecular Biology and Biochemistry of the Coagulant Factor and Pathways of Hemostasis, in Beutler E, Lichtman MA,Coller BS, Kipps TJ, Seligshon U (eds): Williams Hematology. Newyork, McGrawHill, 2001, pp 1409-1434.
  • Yarat A: Tromboplastik Aktivite (Doku faktörü aktivitesi) 4.Ulusal Tromboz,Hemostaz ve Anjioloji Kongresi 26-28 Eylül 2003- Trakya Üniversitesi Tıp Fakültesi, Edirne, s.97-105.
  • Jiang X, Konigshey UH, Brombey ME: Formation of tissue factor-Factor VIIa Complex promotor cellular signaling and migration of tumor breast cancer cells. J Thromb Haemost 2003; 1(9): 1972-1976.
  • Ingram GIC, Hills M : Reference method for the one-stage prothrombin-time test on human blood. Thromb Haemostas 1976; 36 : 237-238.
  • Lowry OH, Rosebrough WI, Farr A, Randal RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-275.
  • Laemmli UK Cleavage of structural proteins during the assembly of the head of bacteriofage T4. Nature 1970; 227: 680-685.
  • Atay Z, Topalidis T : Cytodiagnostik der Serösen Höhlen. Atlas und Lehrbuch. Hannover , Wolfgang Pabst verlag, 1994; 336.
  • Grignani G, Mailo A: Cytokines and hemostasis. Haematologica.2000; 73(2):145-150.
  • Hathcock J: Vascular biology. The role of tissue factor Hematol. 2004; 41(1): 30-34.
  • Berckmans RJ, Sturk A, van Tienen LM, Schaap MCL, Nieuwland R: Cell-derived vesicles exposing coagulant tissue factor in saliva. Blood 2011; 117(11): 31172-31180.
  • Thylstrup A, Federskov O.: Textbook of Clinical Cariology. Copenhagen, Munkgoard, 1986; pp 28-45.
  • Jenkins NG: The physiology and biochemistry of mouth. Oxford Blackwell Scientific Publications, 1978; pp284-360.
  • Brand HS, Veerman EC: Saliva and wound healing. Chin J Dent Res  2013;16(1):7-12.
  • Kleinjan A, Böing AN, Sturk A, Nieuwland R: Microparticles in vascular disorders: How tissue factor-exposing vesicles contribute to pathology and physiology. Thromb Res 2012; 130: 71–73.
There are 23 citations in total.

Details

Primary Language English
Journal Section Makaleler
Authors

Tuğba Tunalı Akbay

Ayşen Yarat

Tiraje Celkan This is me

Serap Akyüz This is me

Rabia Pişiriciler This is me

İnci Yıldız This is me

Publication Date December 19, 2014
Published in Issue Year 2013 Volume: 1 Issue: 2

Cite

APA Tunalı Akbay, T., Yarat, A., Celkan, T., Akyüz, S., et al. (2014). Salivary Thromboplastic Activity in Children with Cancer. Marmara Dental Journal, 1(2), 67-70. https://doi.org/10.12990/ MDJ.201317512
AMA Tunalı Akbay T, Yarat A, Celkan T, Akyüz S, Pişiriciler R, Yıldız İ. Salivary Thromboplastic Activity in Children with Cancer. Marmara Dental Journal. December 2014;1(2):67-70. doi:10.12990/ MDJ.201317512
Chicago Tunalı Akbay, Tuğba, Ayşen Yarat, Tiraje Celkan, Serap Akyüz, Rabia Pişiriciler, and İnci Yıldız. “Salivary Thromboplastic Activity in Children With Cancer”. Marmara Dental Journal 1, no. 2 (December 2014): 67-70. https://doi.org/10.12990/ MDJ.201317512.
EndNote Tunalı Akbay T, Yarat A, Celkan T, Akyüz S, Pişiriciler R, Yıldız İ (December 1, 2014) Salivary Thromboplastic Activity in Children with Cancer. Marmara Dental Journal 1 2 67–70.
IEEE T. Tunalı Akbay, A. Yarat, T. Celkan, S. Akyüz, R. Pişiriciler, and İ. Yıldız, “Salivary Thromboplastic Activity in Children with Cancer”, Marmara Dental Journal, vol. 1, no. 2, pp. 67–70, 2014, doi: 10.12990/ MDJ.201317512.
ISNAD Tunalı Akbay, Tuğba et al. “Salivary Thromboplastic Activity in Children With Cancer”. Marmara Dental Journal 1/2 (December 2014), 67-70. https://doi.org/10.12990/ MDJ.201317512.
JAMA Tunalı Akbay T, Yarat A, Celkan T, Akyüz S, Pişiriciler R, Yıldız İ. Salivary Thromboplastic Activity in Children with Cancer. Marmara Dental Journal. 2014;1:67–70.
MLA Tunalı Akbay, Tuğba et al. “Salivary Thromboplastic Activity in Children With Cancer”. Marmara Dental Journal, vol. 1, no. 2, 2014, pp. 67-70, doi:10.12990/ MDJ.201317512.
Vancouver Tunalı Akbay T, Yarat A, Celkan T, Akyüz S, Pişiriciler R, Yıldız İ. Salivary Thromboplastic Activity in Children with Cancer. Marmara Dental Journal. 2014;1(2):67-70.