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Lentinula edodes (Berk.) Pegler 1976’ in Misel Gelişmesine Humik Maddeler ve Giberellik Asidin Etkisinin Araştırılması

Year 2020, Volume: 11 Issue: 1, 40 - 49, 27.04.2020

Abstract

Bu çalışmada Lentinula edodes’in misel gelişimi üzerine hümik maddeler ve giberellik asidin (GA3) etkisi araştırılmıştır. Malt ekstrakt agar (MEA) ve patates dekstroz agar (PDA) besi yeri olarak kullanılmış olup, bu besiyer ortamlarına, hümik madde (HM) ve giberellik asidin (GA3) belirli dozları ilave edilerek otoklavda 121°C’de 15 dk steril edilmiştir. Pastör fırınında steril edilmiş petrilere steril güvenlik kabini içerisinde besi yerleri dökülmüştür. Besi yerleri katılaştıktan sonra aynı kabin içerisinde misel ihtiva eden besi yeri aktarımı yapılmıştır. Bu işlemden sonra petriler etüve alınmıştır. Belli aralıklarla miselyum gelişmeleri alansal olarak çizilmiştir. Ön çalışma olarak besiyerlerine %0, %1, %3, %5, %7, %9 oranlarında hümik madde ve giberellik asidin (GA3) ilavesi yapılmış ve bu oranlarda yapılan uygulama sonuçlarına göre, çalışma denemelerinin %0, %0.5, %1, %1.5 oranlarında yapılmasına karar verilmiştir. Bu çalışmada MEA ve PDA besiyerlerinin hazırlanması esnasında %0, %0.5, %1 ve %1.5 oranında hümik madde ve giberellik asidin (GA3) eklenerek petrilere aktarılmıştır. Lentinula edodes’in misel gelişim miktarları ve yoğunlukları incelenmiştir. Denemeler 3 tekrarlı olarak yapılmıştır. Deneme sonuçlarına göre yapılan varyans analizlerine göre ortalamalar arasındaki farklar istatistiki olarak önemli bulunmuştur (P<0.01). %0, %0.5, %1 ve %1.5 oranında hümik madde ve giberellik asit (GA3) uygulamaları yönünden değerlendirme yapıldığında gelişim miktarı ve katkı maddeleri oranları arasında yüksek bir korelasyon tespit edilememiş olup, MEA besi ortamında 0.5 ve 1.5’ lik hümik madde uygulamasında nispeten yüksek gelişim olduğu görülmüştür (R² = 0.370).

Supporting Institution

S. Ü. BAP

Project Number

18201038

Thanks

Bu çalışmayı 18201038 nolu proje ile destekleyen Selçuk Üniversitesi BAP Koordinatörlüğü’ne teşekkür ederiz.

References

  • Adani, F., Genevini, P., Zaccheo, P., and Zocchi, G. (1998). The effect of commercial humic acid on tomato plant growth and mineral nutrition. Journal of plant nutrition, 21(3), 561-575.
  • Aşık, B.B., Turan, M.A. Çelik, H. and Katkat, A.V. (2009). Effects of humic substances on plant growth and mineral nutrients uptake of wheat (Triticum durum cv Salihli) under conditions of salinity. Asian Journal of CropScience. 1 (2): 87-95
  • Ay, Z., Kaşık, G. ve Alkan, S. (2019). Pleurotus ostreatus’un misel gelişmesine sitokininlerin etkisi. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi (Basımda).
  • Bohme, M. and Thi Lua, H. (1997). Influence of mineral and organic treatments in the rhizosphere on the growth of tomato plants, Acta Hortic. 450: 161-168
  • Böhme, M., and Lua, H. (1996, September). Influence of mineral and organic treatments in the rhizosphere on the growth of tomato plants. In International Symposium Growing Media and Plant Nutrition in Horticulture 450 (pp. 161-168).
  • Chen, Y., and Aviad, T. (1990). Effects of humic substances on plant growth 1. Humic substances in soil and crop sciences: Selected readings, (humicsubstances), 161-186.
  • Eyheraguibel, B., Silvestre, J., and Morard, P. (2008). Effects of humic substances derived from organic waste enhancement on the growth and mineral nutrition of maize. Bioresource technology, 99(10), 4206-4212
  • Kalmıs, E., ve Kalyoncu, F.(2007). Lentinula edodes’ in Misel Gelişim Hızı Üzerine Meşe Odunu Parça Büyüklüğünün Etkisi. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, 7(2), 45-52.
  • Madrap, B.A., Bhalerao, R.K., Hudge, V.S., and Siddique, M.A. (1992). Effect of foliar spray of growth regulators on yield of sunflower. Annals Plant Physiol, 6(2), 217-221.
  • Nardi S, Pizzeghello D, Muscolo A, Vianello A. (2002). Physiological effects of humic substances on higher plants. Soil Biol. and Bioch. 34:1527-1536.
  • Önay, A.O., Kaşık, G., Alkan, S., Öztürk, C.(2018), Pleurotus ostreatus’un Misel Gelişmesine Humik Maddelerin Etkisinin Araştırılması. II. International Eurasian Agriculture and Natural Sciences Congress, sayfa:22-29 ,11-15/09/2018-Azerbaycan-Baku.
  • Shunkla, D.S., Deshmukh, P.S., and Wasnik, K.G. (1987). Effect of GA3 on seed setting and seed filling in sunflower. Seed Research, 15(2), 138-142.
  • Saruhan, V., Kusvuran, A. and Kokten, K. (2011). The effect of different replications of humic acid fertilization on yield performances of common vetch (Vicia sativa L.). African Journal of Biotechnology, 10, 5587-5592.
  • Saruhan, V., Kusvuran, A., and Kokten, K. (2011). The effect of different replications of humic acid fertilization on yield performances of common vetch (Vicia sativa L.). African Journal of Biotechnology, 10(29), 5587-5592.
  • Sharif, M., Khattak, R.A., and Sarir, M.S. (2002). Effect of different levels of lignitic coal derived humic acid on growth of maize plants. Communications in soil science and plant analysis, 33(19-20), 3567-3580.
  • Stamets, P., (1993). Growing Gourmet & Medicinal Mushrooms.Ten Speed Press, pp:554.

Investigation of the Effect of Humic Substances and Gibberellic Acid on Mycelium Growth of Lentinula edodes (Berk.) Pegler 1976

Year 2020, Volume: 11 Issue: 1, 40 - 49, 27.04.2020

Abstract

Abstract In this study, the effect of humic substances and gibberellic acid (GA3) on mycelial development of Lentinula edodes was investigated. Malt extract agar (MEA) and potato dextrose agar (PDA) were used as feedstock in the study and malt extract agar and potato dextrose agar were sterilized by autoclaving at 121°C for 15 min with the addition of specific doses of agaric material. In the Pasteur oven, nutrients were poured into sterile petri dishes in the sterile safety cabinet. After the feedstock sites solidified, the transfer of the media containing the mycelium was carried out in the same cabin. After this process, the petri dishes were taken to the incubation oven. At certain intervals mycelial developments were drawn spatially. As a preliminary study, 0%, 1%, 3%, 5%, 7% and 9% of humic substance and gibberellic acid (GA3) were added to feedstock and according to the results of the applications made at these rates, it has been decided to make the working trials at 0%, 0.5%, 1%, 1.5%. In this study, malt extract agar and potato dextrose agar supplemented with 0%, 0.5%, 1% and 1.5% of humic substance and gibberellic acid (GA3) were added to petri dishes. Micelle growth amounts and densities of Lentinula edodes were investigated. The experiments were done in 3 replicates. According to the analysis of variance with trial results the differences between the means were found to be statistically significant (P <0.01). When %0, 0.5%, 1% and 1.5% of humic substance and gibberellic acid (GA3) applications were evaluated, no high correlation was found between the amount of growth and additives and only a relatively high improvement was observed in the application of 0.5% and 1.5% humic substance in MEA feedstock (R² = 0.370).

Project Number

18201038

References

  • Adani, F., Genevini, P., Zaccheo, P., and Zocchi, G. (1998). The effect of commercial humic acid on tomato plant growth and mineral nutrition. Journal of plant nutrition, 21(3), 561-575.
  • Aşık, B.B., Turan, M.A. Çelik, H. and Katkat, A.V. (2009). Effects of humic substances on plant growth and mineral nutrients uptake of wheat (Triticum durum cv Salihli) under conditions of salinity. Asian Journal of CropScience. 1 (2): 87-95
  • Ay, Z., Kaşık, G. ve Alkan, S. (2019). Pleurotus ostreatus’un misel gelişmesine sitokininlerin etkisi. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi (Basımda).
  • Bohme, M. and Thi Lua, H. (1997). Influence of mineral and organic treatments in the rhizosphere on the growth of tomato plants, Acta Hortic. 450: 161-168
  • Böhme, M., and Lua, H. (1996, September). Influence of mineral and organic treatments in the rhizosphere on the growth of tomato plants. In International Symposium Growing Media and Plant Nutrition in Horticulture 450 (pp. 161-168).
  • Chen, Y., and Aviad, T. (1990). Effects of humic substances on plant growth 1. Humic substances in soil and crop sciences: Selected readings, (humicsubstances), 161-186.
  • Eyheraguibel, B., Silvestre, J., and Morard, P. (2008). Effects of humic substances derived from organic waste enhancement on the growth and mineral nutrition of maize. Bioresource technology, 99(10), 4206-4212
  • Kalmıs, E., ve Kalyoncu, F.(2007). Lentinula edodes’ in Misel Gelişim Hızı Üzerine Meşe Odunu Parça Büyüklüğünün Etkisi. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, 7(2), 45-52.
  • Madrap, B.A., Bhalerao, R.K., Hudge, V.S., and Siddique, M.A. (1992). Effect of foliar spray of growth regulators on yield of sunflower. Annals Plant Physiol, 6(2), 217-221.
  • Nardi S, Pizzeghello D, Muscolo A, Vianello A. (2002). Physiological effects of humic substances on higher plants. Soil Biol. and Bioch. 34:1527-1536.
  • Önay, A.O., Kaşık, G., Alkan, S., Öztürk, C.(2018), Pleurotus ostreatus’un Misel Gelişmesine Humik Maddelerin Etkisinin Araştırılması. II. International Eurasian Agriculture and Natural Sciences Congress, sayfa:22-29 ,11-15/09/2018-Azerbaycan-Baku.
  • Shunkla, D.S., Deshmukh, P.S., and Wasnik, K.G. (1987). Effect of GA3 on seed setting and seed filling in sunflower. Seed Research, 15(2), 138-142.
  • Saruhan, V., Kusvuran, A. and Kokten, K. (2011). The effect of different replications of humic acid fertilization on yield performances of common vetch (Vicia sativa L.). African Journal of Biotechnology, 10, 5587-5592.
  • Saruhan, V., Kusvuran, A., and Kokten, K. (2011). The effect of different replications of humic acid fertilization on yield performances of common vetch (Vicia sativa L.). African Journal of Biotechnology, 10(29), 5587-5592.
  • Sharif, M., Khattak, R.A., and Sarir, M.S. (2002). Effect of different levels of lignitic coal derived humic acid on growth of maize plants. Communications in soil science and plant analysis, 33(19-20), 3567-3580.
  • Stamets, P., (1993). Growing Gourmet & Medicinal Mushrooms.Ten Speed Press, pp:554.
There are 16 citations in total.

Details

Primary Language Turkish
Journal Section RESEARCH ARTICLE
Authors

Fatma Kayahan 0000-0001-6506-4492

Gıyasettin Kaşık 0000-0001-8304-6554

Nurettin Kayahan 0000-0002-9031-0699

Project Number 18201038
Publication Date April 27, 2020
Published in Issue Year 2020 Volume: 11 Issue: 1

Cite

APA Kayahan, F., Kaşık, G., & Kayahan, N. (2020). Lentinula edodes (Berk.) Pegler 1976’ in Misel Gelişmesine Humik Maddeler ve Giberellik Asidin Etkisinin Araştırılması. Mantar Dergisi, 11(1), 40-49. https://doi.org/10.30708/mantar.610869

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