Research Article
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Sıçanlarda Bor ve Vitamin C Takviyesinin Hematolojik ve Lipid Parametrelerine Etkileri

Year 2023, Volume: 16 Issue: 4, 597 - 605, 31.12.2023

Bestami Kemal Gümüşay Abdullah Eryavuz

https://doi.org/10.30607/kvj.1349851

Abstract

Bu çalışmanın amacı, bor (B) ve vitamin C'nin birlikte uygulanmasının kandaki trigliserit, kolesterol, HDL ve LDL'nin seviyeleri ile hematolojik parametrelerine etkisini belirlemektir. Çalışmada, 32 adet 250-300 gr ağırlığında Wistar Albino cinsi erkek sıçan alınarak 4 adet çalışma grubu oluşturulmuştur. Çalışma grupları Kontrol, B, Vitamin C ve Vitamin C + B olarak şekillendi. Vitamin C ve B grubu sıçanlara sırasıyla 200 mg.kg.-1 C vitamini oral, bor 5 mg.kg.-1 intraperitoneal olarak verildi. Vitamin C+B grubundaki sıçanlara C vitamini oral yoldan 200 mg.kg.-1, intraperitoneal olarak 5 mg.kg.-1 B verildi. Toplam 35 gün süren deneme sonunda anestezi altında kalpten alınan kan örneklerinde; trigliserid, kolesterol, HDL ve LDL düzeyleri ile hematolojik parametreler ölçüldü. Yapılan uygulamaların trigliserid, kolesterol, HDL ve LDL`nin seviyelerine etki etmediği tespit edildi. Hem vitamin C hem de B uygulamalarının eritrosit sayısı, hematokrit değer ve hemoglobin düzeylerine etkisinin olmadığı, vitamin C'nin B ile takviyesinin birlikte kullanılmasının lökosit sayısını düşürdüğü ve trombosit sayısını ise artırdığı bulundu. Sonuç olarak, antioksidan özelliklere sahip vitamin C ve B’un birlikte kullanılmaları serum lipid düzeylerini değiştirmediği ancak kan lökosit ve trombosit sayısını etkilediği gözlendi.

Keywords

Bor HDL Hematolojik Parametreler Sıçan Vitamin C

Supporting Institution

Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

20.SAĞ.BİL.38

References

  • Armstrong, T. A., & Spears, J. W. (2001). Effect of dietary boron on growth performance, calcium and phosphorus metabolism, and bone mechanical properties in growing barrows. Journal of animal science, 79(12), 3120-3127.
  • Armstrong, T. A., Spears, J. W., Crenshaw, TD., & Nielsen, FH., (2000). Boron supplementation of a semipurified diet for weanling pigs improves feed efficiency and bone strength characteristics and alters plasma lipid metabolites. 130(10):2575-81. doi: 10.1093/jn/130.10.2575.
  • Ashor, A. W., Siervo, M., van der Velde, F., Willis, N. D., & Mathers, J. C. (2016). Systematic review and meta-analysis of randomised controlled trials testing the effects of vitamin C supplementation on blood lipids. Clinical nutrition (Edinburgh, Scotland), 35(3), 626–637. https://doi.org/10.1016/j.clnu.2015.05.021
  • Başeğmez, M., & Eryavuz, A. (2021). Sıçanlarda oral yüksek doz vitamin C takviyesinin serum vitamin C, leptin ve kortizol parametrelerine etkisi. Kocatepe Veterinary Journal, 14(3), 339-345.
  • Başoğlu, A., Sevinç, M., Güzelbektaş, H. and Civelek, T. (2000): Short communication: Effect of Borax on serum lipid profile in dogs. Online J. Vet. Res. 4, 153–156.
  • Bello, M., Guadarrama-García, C., Velasco-Silveyra, L. M., Farfán-García, E. D., & Soriano-Ursúa, M. A. (2018). Several effects of boron are induced by uncoupling steroid hormones from their transporters in blood. Medical Hypotheses, 118, 78-83.
  • Biţă, A., Scorei, I. R., Bălşeanu, T. A., Ciocîlteu, M. V., Bejenaru, C., Radu, A., ... & Benner, S. A. (2022). New insights into boron essentiality in humans and animals. International Journal of Molecular Sciences, 23(16), 9147.
  • Cortes, VA., Busso, D., Maiz, A., Arteaga, A., Nervi, F., & Rigotti, A., (2014). Phyiological and pathological implications of cholesterol. Front. Biosci. (Landmark Ed.);19(3):416-28. doi: 10.2741/4216.
  • Durmuş, İ., İnce, S., Salim, M.N., Eryavuz, A., & Küçükkurt, İ. (2018). Gentamisin verilen sıçanlara bor uygulamasının hematolojik parametre düzeylerine etkileri. Kocatepe Veterinary Journal, 11(2), 140-147.
  • Eren, M., & Uyanik, F. (2007). Influence of dietary boron supplementation on some serum metabolites and egg-yolk cholesterol in laying hens. Acta Veterinaria Hungarica, 55(1), 29-39.
  • Etim NN, Williams ME, Akpabio U, Offiong EE. (2014). Haematological parameters and factors affecting their values. Agricultural Science. 2(1):37-47.
  • Farfán-García, E. D., Castillo-Mendieta, N. T., Ciprés-Flores, F. J., Padilla-Martínez, I. I., Trujillo-Ferrara, J. G., & Soriano-Ursúa, M. A. (2016). Current data regarding the structure-toxicity relationship of boron-containing compounds. Toxicology letters, 258, 115-125.
  • Farombi, E.O., Ugwuezunmba, M.C., Ezenwadu T.T., Ovevemi M.O., & Ekor M., (2008). Tetracycline-induced reproductive toxicity in male rats: effects of vitamin C and N-acetylcysteine Experimental and Toxicologic Pathology, 60 (1), 77-85.
  • Giannetto, C., Fazio, F., Nava, V., Arfuso, F., Piccione, G., Coelho, C., ... & Licata, P. (2022). Data on multiple regression analysis between boron, nickel, arsenic, antimony, and biological substrates in horses: The role of hematological biomarkers. Journal of Biochemical and Molecular Toxicology, 36(2), e22955.
  • Harrison, F. E., & May, J. M. (2009). Vitamin C function in the brain: vital role of the ascorbate transporter SVCT2. Free Radical Biology and Medicine, 46(6), 719-730.
  • İnce, S., Kucukkurt, I., Cigerci, IH., Fidan, AF., & Eryavuz, A., (2010). The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. J. Trace Elem. Med. Biol. 2010; 24: 161-164.
  • Kabu, M., & Akosman, M. S. (2013). Biological effects of boron. Reviews of environmental contamination and toxicology, 225: 57-75.
  • Kaya, H. & Macit, M. (2020). Yumurtlamanın Son Dönemindeki Yumurtacı Tavukların Rasyonlarına Bor (Orthoborik Asit) İlavesinin Serum Lipid ve Protein Profili Üzerine Etkisi . Tekirdağ Ziraat Fakültesi Dergisi , 17 (1) , 66-76 . DOI: 10.33462/jotaf.488195
  • Kaya, Z., & Çenesiz, G. Z. (2010). Deney hayvanlarının fizyolojisi. In: Aksoy A, Kolbakır F, Hökelek M editors. Laboratuvar hayvanları. Ondokuz Mayıs Üniversitesi Yayınları 2010; p.42.
  • Kurtoğlu, F., Kurtoğlu, V., Celik, I., Kececi, T., & Nizamlioğlu, M. (2005). Effects of dietary boron supplementation on some biochemical parameters, peripheral blood lymphocytes, splenic plasma cells and bone characteristics of broiler chicks given diets with adequate or inadequate cholecalciferol (vitamin D3) content. British poultry science, 46(1), 87-96.
  • Mızrak, C., & Ceylan, M. (2009). Damızlık yumurtacı tavuk yemlerine farklı seviye ve formda bor ilavesinin performans, kemik gelişimi ve bazı kan parametreleri üzerine etkisi 6. Ulusal Zootekni Bilim Kong, 60(69), 24-26.
  • Naghii, M. R., & Samman, S. (1993). Role of boron in nutrition and metabolism. Prog. Fd. Nutr. Sci., 14, 331-349.
  • Naghii, M. R., & Samman, S. (1997). The effect of boron on plasma testosterone and plasma lipids in rats. Nutrition Research, 17(3), 523-531.
  • Newkirk, C. E., Gagnon, Z. E., & Pavel Sizemore, I. E. (2014). Comparative study of hematological responses to platinum group metals, antimony and silver nanoparticles in animal models. Journal of Environmental Science and Health, Part A, 49(3), 269-280.
  • Noyan, A. (1989). Yaşamda ve Hekimlikte Fizyoloji. Hacettepe Kitapçılık, Ankara, 1989.
  • Olgun, O. (2011). Yumurtacı tavuk rasyonlarına farklı seviyelerde ilave edilen bor ve bakırın performans, yumurta kabuk kalitesi, yumurta sarısı kolesterolü ve kemiğin biyomekanik özelliklerine etkisi. Doktora Tezi, Selçuk Üniversitesi Fen Bilimleri Enstitüsü, Konya.
  • O'Malley, P. G., Arnold, M. J., Kelley, C., Spacek, L., Buelt, A., Natarajan, S., . . . Downs, J. R. (2020). Management of Dyslipidemia for Cardiovascular Disease Risk Reduction: Synopsis of the 2020 Updated U.S. Department of Veterans Affairs and U.S. Department of Defense Clinical Practice Guideline. Annals of Internal Medicine, 173(10), 822-829. doi:10.7326/M20-4648
  • Oyawoye, B. M., & Ogunkunle, H. N. (2004). Biochemical and haematological reference values in normal experimental animals. New York: Mason, 212-216.
  • Patak, P., Willenberg, H. S., & Bornstein, S. R. (2004). Vitamin C is an important cofactor for both adrenal cortex and adrenal medulla. Endocrine research, 30(4), 871-875.
  • Sheng, M. H. C., Janette Taper, L., Veit, H., Qian, H., Ritchey, S. J., & William Lau, K. H. (2001). Dietary boron supplementation enhanced the action of estrogen, but not that of parathyroid hormone, to improve trabecular bone quality in ovariectomized rats. Biological trace element research, 82, 109-123.
  • Stokinger, H. E. (1981). In: G.D. Clayton and F.E. Clayton (eds.),Patty's Industrial Hygiene and Toxicology, 3rd Edn. John Wiley and Sons, Toronto, Canada, Pp: 1749-1769.
  • Şimşek, H. (2011). Etlik piliç rasyonlarına bor (ortoborik asit) ilavesinin performans değerleri ile tibia mineral konsantrasyonu ve bazı kan parametreleri üzerine etkisi. Yüksek Lisans Tezi, Atatürk Üniv. Fen Bil.Enst., Erzurum.
  • Yıldırım, S., Celikezen, F. C., Oto, G., Sengul, E., Bulduk, M., Tasdemir, M., & Ali Cinar, D. (2018). An investigation of protective effects of litium borate on blood and histopathological parameters in acute cadmium-induced rats. Biological trace element research, 182, 287-294.

Effects of Supplementation of Boron and Vitamin C on Lipid and Hematological Parameters in Rats

Year 2023, Volume: 16 Issue: 4, 597 - 605, 31.12.2023

Bestami Kemal Gümüşay Abdullah Eryavuz

https://doi.org/10.30607/kvj.1349851

Abstract

The aim of this study is to determine the effects of the co-administration of boron (B) and vitamin C on blood triglyceride, cholesterol, HDL and LDL levels and hematological parameters. In the study, four study groups created with 32 Wistar Albino male rats weighing 250-300 g. The experimental groups were shaped as Control, Boron, Vitamin C and Vitamin C + Boron. Vitamin C and boron were administered 200 mg.kg.-1of vitamin C orally, and boron at an amount of 5 mg.kg-1 intraperitoneally, respectively in the rats of the Vitamin C and Boron gorups. The rats in theVitamin C+Boron group were given at 200 mg.kg.-1 of vitamin C orally, and 5 mg.kg.-1of boron intraperitoneally. At the end of the trial, which was for total of 35 days, triglyceride, cholesterol, HDL and LDL levels and hematological parameters were measured in blood samples taken from the heart under anesthesia. It observed that the applications of vitamin C and boron had no effects on the plasma levels of triglycerides, cholesterol, HDL and LDL. Both vitamin C and boron applications had no effect on the erythrocyte count, hematocrit value and hemoglobin levels, however the combination of vitamin C and boron supplementation decreased the leukocyte count, and there was an increase on the platelet count. In conclusion, the combination of vitamin C and boron with antioxidant properties did not change plasma lipid levels, but it affected blood leukocyte and platelet counts.

Keywords

Boron HDL Hematological parameters Rat Vitamin C

Project Number

20.SAĞ.BİL.38

References

  • Armstrong, T. A., & Spears, J. W. (2001). Effect of dietary boron on growth performance, calcium and phosphorus metabolism, and bone mechanical properties in growing barrows. Journal of animal science, 79(12), 3120-3127.
  • Armstrong, T. A., Spears, J. W., Crenshaw, TD., & Nielsen, FH., (2000). Boron supplementation of a semipurified diet for weanling pigs improves feed efficiency and bone strength characteristics and alters plasma lipid metabolites. 130(10):2575-81. doi: 10.1093/jn/130.10.2575.
  • Ashor, A. W., Siervo, M., van der Velde, F., Willis, N. D., & Mathers, J. C. (2016). Systematic review and meta-analysis of randomised controlled trials testing the effects of vitamin C supplementation on blood lipids. Clinical nutrition (Edinburgh, Scotland), 35(3), 626–637. https://doi.org/10.1016/j.clnu.2015.05.021
  • Başeğmez, M., & Eryavuz, A. (2021). Sıçanlarda oral yüksek doz vitamin C takviyesinin serum vitamin C, leptin ve kortizol parametrelerine etkisi. Kocatepe Veterinary Journal, 14(3), 339-345.
  • Başoğlu, A., Sevinç, M., Güzelbektaş, H. and Civelek, T. (2000): Short communication: Effect of Borax on serum lipid profile in dogs. Online J. Vet. Res. 4, 153–156.
  • Bello, M., Guadarrama-García, C., Velasco-Silveyra, L. M., Farfán-García, E. D., & Soriano-Ursúa, M. A. (2018). Several effects of boron are induced by uncoupling steroid hormones from their transporters in blood. Medical Hypotheses, 118, 78-83.
  • Biţă, A., Scorei, I. R., Bălşeanu, T. A., Ciocîlteu, M. V., Bejenaru, C., Radu, A., ... & Benner, S. A. (2022). New insights into boron essentiality in humans and animals. International Journal of Molecular Sciences, 23(16), 9147.
  • Cortes, VA., Busso, D., Maiz, A., Arteaga, A., Nervi, F., & Rigotti, A., (2014). Phyiological and pathological implications of cholesterol. Front. Biosci. (Landmark Ed.);19(3):416-28. doi: 10.2741/4216.
  • Durmuş, İ., İnce, S., Salim, M.N., Eryavuz, A., & Küçükkurt, İ. (2018). Gentamisin verilen sıçanlara bor uygulamasının hematolojik parametre düzeylerine etkileri. Kocatepe Veterinary Journal, 11(2), 140-147.
  • Eren, M., & Uyanik, F. (2007). Influence of dietary boron supplementation on some serum metabolites and egg-yolk cholesterol in laying hens. Acta Veterinaria Hungarica, 55(1), 29-39.
  • Etim NN, Williams ME, Akpabio U, Offiong EE. (2014). Haematological parameters and factors affecting their values. Agricultural Science. 2(1):37-47.
  • Farfán-García, E. D., Castillo-Mendieta, N. T., Ciprés-Flores, F. J., Padilla-Martínez, I. I., Trujillo-Ferrara, J. G., & Soriano-Ursúa, M. A. (2016). Current data regarding the structure-toxicity relationship of boron-containing compounds. Toxicology letters, 258, 115-125.
  • Farombi, E.O., Ugwuezunmba, M.C., Ezenwadu T.T., Ovevemi M.O., & Ekor M., (2008). Tetracycline-induced reproductive toxicity in male rats: effects of vitamin C and N-acetylcysteine Experimental and Toxicologic Pathology, 60 (1), 77-85.
  • Giannetto, C., Fazio, F., Nava, V., Arfuso, F., Piccione, G., Coelho, C., ... & Licata, P. (2022). Data on multiple regression analysis between boron, nickel, arsenic, antimony, and biological substrates in horses: The role of hematological biomarkers. Journal of Biochemical and Molecular Toxicology, 36(2), e22955.
  • Harrison, F. E., & May, J. M. (2009). Vitamin C function in the brain: vital role of the ascorbate transporter SVCT2. Free Radical Biology and Medicine, 46(6), 719-730.
  • İnce, S., Kucukkurt, I., Cigerci, IH., Fidan, AF., & Eryavuz, A., (2010). The effects of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. J. Trace Elem. Med. Biol. 2010; 24: 161-164.
  • Kabu, M., & Akosman, M. S. (2013). Biological effects of boron. Reviews of environmental contamination and toxicology, 225: 57-75.
  • Kaya, H. & Macit, M. (2020). Yumurtlamanın Son Dönemindeki Yumurtacı Tavukların Rasyonlarına Bor (Orthoborik Asit) İlavesinin Serum Lipid ve Protein Profili Üzerine Etkisi . Tekirdağ Ziraat Fakültesi Dergisi , 17 (1) , 66-76 . DOI: 10.33462/jotaf.488195
  • Kaya, Z., & Çenesiz, G. Z. (2010). Deney hayvanlarının fizyolojisi. In: Aksoy A, Kolbakır F, Hökelek M editors. Laboratuvar hayvanları. Ondokuz Mayıs Üniversitesi Yayınları 2010; p.42.
  • Kurtoğlu, F., Kurtoğlu, V., Celik, I., Kececi, T., & Nizamlioğlu, M. (2005). Effects of dietary boron supplementation on some biochemical parameters, peripheral blood lymphocytes, splenic plasma cells and bone characteristics of broiler chicks given diets with adequate or inadequate cholecalciferol (vitamin D3) content. British poultry science, 46(1), 87-96.
  • Mızrak, C., & Ceylan, M. (2009). Damızlık yumurtacı tavuk yemlerine farklı seviye ve formda bor ilavesinin performans, kemik gelişimi ve bazı kan parametreleri üzerine etkisi 6. Ulusal Zootekni Bilim Kong, 60(69), 24-26.
  • Naghii, M. R., & Samman, S. (1993). Role of boron in nutrition and metabolism. Prog. Fd. Nutr. Sci., 14, 331-349.
  • Naghii, M. R., & Samman, S. (1997). The effect of boron on plasma testosterone and plasma lipids in rats. Nutrition Research, 17(3), 523-531.
  • Newkirk, C. E., Gagnon, Z. E., & Pavel Sizemore, I. E. (2014). Comparative study of hematological responses to platinum group metals, antimony and silver nanoparticles in animal models. Journal of Environmental Science and Health, Part A, 49(3), 269-280.
  • Noyan, A. (1989). Yaşamda ve Hekimlikte Fizyoloji. Hacettepe Kitapçılık, Ankara, 1989.
  • Olgun, O. (2011). Yumurtacı tavuk rasyonlarına farklı seviyelerde ilave edilen bor ve bakırın performans, yumurta kabuk kalitesi, yumurta sarısı kolesterolü ve kemiğin biyomekanik özelliklerine etkisi. Doktora Tezi, Selçuk Üniversitesi Fen Bilimleri Enstitüsü, Konya.
  • O'Malley, P. G., Arnold, M. J., Kelley, C., Spacek, L., Buelt, A., Natarajan, S., . . . Downs, J. R. (2020). Management of Dyslipidemia for Cardiovascular Disease Risk Reduction: Synopsis of the 2020 Updated U.S. Department of Veterans Affairs and U.S. Department of Defense Clinical Practice Guideline. Annals of Internal Medicine, 173(10), 822-829. doi:10.7326/M20-4648
  • Oyawoye, B. M., & Ogunkunle, H. N. (2004). Biochemical and haematological reference values in normal experimental animals. New York: Mason, 212-216.
  • Patak, P., Willenberg, H. S., & Bornstein, S. R. (2004). Vitamin C is an important cofactor for both adrenal cortex and adrenal medulla. Endocrine research, 30(4), 871-875.
  • Sheng, M. H. C., Janette Taper, L., Veit, H., Qian, H., Ritchey, S. J., & William Lau, K. H. (2001). Dietary boron supplementation enhanced the action of estrogen, but not that of parathyroid hormone, to improve trabecular bone quality in ovariectomized rats. Biological trace element research, 82, 109-123.
  • Stokinger, H. E. (1981). In: G.D. Clayton and F.E. Clayton (eds.),Patty's Industrial Hygiene and Toxicology, 3rd Edn. John Wiley and Sons, Toronto, Canada, Pp: 1749-1769.
  • Şimşek, H. (2011). Etlik piliç rasyonlarına bor (ortoborik asit) ilavesinin performans değerleri ile tibia mineral konsantrasyonu ve bazı kan parametreleri üzerine etkisi. Yüksek Lisans Tezi, Atatürk Üniv. Fen Bil.Enst., Erzurum.
  • Yıldırım, S., Celikezen, F. C., Oto, G., Sengul, E., Bulduk, M., Tasdemir, M., & Ali Cinar, D. (2018). An investigation of protective effects of litium borate on blood and histopathological parameters in acute cadmium-induced rats. Biological trace element research, 182, 287-294.
There are 33 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Anatomy and Physiology, Veterinary Biochemistry
Journal Section RESEARCH ARTICLE
Authors

Bestami Kemal Gümüşay 0009-0007-9567-2529

Abdullah Eryavuz 0000-0001-8602-2400

Project Number 20.SAĞ.BİL.38
Early Pub Date December 20, 2023
Publication Date December 31, 2023
Acceptance Date December 13, 2023
Published in Issue Year 2023 Volume: 16 Issue: 4

Cite

APA Gümüşay, B. K., & Eryavuz, A. (2023). Sıçanlarda Bor ve Vitamin C Takviyesinin Hematolojik ve Lipid Parametrelerine Etkileri. Kocatepe Veterinary Journal, 16(4), 597-605. https://doi.org/10.30607/kvj.1349851
AMA Gümüşay BK, Eryavuz A. Sıçanlarda Bor ve Vitamin C Takviyesinin Hematolojik ve Lipid Parametrelerine Etkileri. kvj. December 2023;16(4):597-605. doi:10.30607/kvj.1349851
Chicago Gümüşay, Bestami Kemal, and Abdullah Eryavuz. “Sıçanlarda Bor Ve Vitamin C Takviyesinin Hematolojik Ve Lipid Parametrelerine Etkileri”. Kocatepe Veterinary Journal 16, no. 4 (December 2023): 597-605. https://doi.org/10.30607/kvj.1349851.
EndNote Gümüşay BK, Eryavuz A (December 1, 2023) Sıçanlarda Bor ve Vitamin C Takviyesinin Hematolojik ve Lipid Parametrelerine Etkileri. Kocatepe Veterinary Journal 16 4 597–605.
IEEE B. K. Gümüşay and A. Eryavuz, “Sıçanlarda Bor ve Vitamin C Takviyesinin Hematolojik ve Lipid Parametrelerine Etkileri”, kvj, vol. 16, no. 4, pp. 597–605, 2023, doi: 10.30607/kvj.1349851.
ISNAD Gümüşay, Bestami Kemal - Eryavuz, Abdullah. “Sıçanlarda Bor Ve Vitamin C Takviyesinin Hematolojik Ve Lipid Parametrelerine Etkileri”. Kocatepe Veterinary Journal 16/4 (December 2023), 597-605. https://doi.org/10.30607/kvj.1349851.
JAMA Gümüşay BK, Eryavuz A. Sıçanlarda Bor ve Vitamin C Takviyesinin Hematolojik ve Lipid Parametrelerine Etkileri. kvj. 2023;16:597–605.
MLA Gümüşay, Bestami Kemal and Abdullah Eryavuz. “Sıçanlarda Bor Ve Vitamin C Takviyesinin Hematolojik Ve Lipid Parametrelerine Etkileri”. Kocatepe Veterinary Journal, vol. 16, no. 4, 2023, pp. 597-05, doi:10.30607/kvj.1349851.
Vancouver Gümüşay BK, Eryavuz A. Sıçanlarda Bor ve Vitamin C Takviyesinin Hematolojik ve Lipid Parametrelerine Etkileri. kvj. 2023;16(4):597-605.
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