Abstract
Subklinik mastitis (SKM),
çiftlik hayvanlarının ciddi bir hastalığıdır. Subklinik mastitis tanısının
pratik, ekonomik ve güvenilir olmasını amaçlayan birçok çalışma vardır. Bu
çalışmada Anadolu mandalarında SKM'in bir göstergesi olarak subklinik mastitis
olan ve olmayan hayvanlarda epidermal büyüme faktörleri (EGF) oranlarının
karşılaştırılması amaçlanmıştır. Çalışma klinik olarak sağlıklı ve gebe olmayan
20 sağlıklı ve 15 mastitisli meme lobundan alınan toplam 35 süt örneğinde
yürütülmüştür. Süt örnekleri alınır alınmaz, laboratuvara transferi
gerçekleştirilmeden önce Kaliforniya mastitis test (CMT) ile saha koşullarında
SKM tespiti yapıldı. Bakteriyel kontaminasyonu belirlemek amacıyla
mikrobiyolojik kültür yapıldı. Tüm örneklerde EGF düzeyi ve somatik hücre
sayısı (SCC) belirlendi. EGF konsantrasyonunun SKM (+) ve SKM (-) grupları
arasındaki karşılaştırma sonucunda, SKM (+) grubunun SKM (-) grubundan daha
yüksek olduğu bulundu. EGF testlerinin subklinik mastitisin belirlenmesinde
yararlı bir araç olabileceği kanısına varılmıştır.
References
- Bradley AJ. Bovine mastitis: An evolving disease. The Veterinary Journal. 2002; 164:117-126.
- Dallard BE, Ruffino V, Heffel S, Calvinho LF. Effect of a biological response modifier on expression of growth factors and cellular proliferation at drying off. . Journal of Dairy Science. 2007; 90:2229-2240.
- Ergun Y, Aslantas O, Kirecci E, Sarıbay MK, Ates AT, Ulku A, Demir C. Prevalence and etiology of SCM in awassi dairy ewes in southern Turkey. Turkish Journal of Veterinary and Animal Science. 2009; 33:477-483.
- Gabadage, K., Chirino-Trejo, M., Campbell, J., Luby, C. Efficacy of recombinant bovine epidermal growth factor in the treatment of experimental subclinical Staphylococcus aureus mastitis in a ewe model. Veterinary Record Open. 2017; 4(1), e000179.
- Guha A, Guha R, Gera S. Comparison of α1-antitrypsin, α1-acid glycoprotein fibrinogen and Nox as indicator of subclinical mastitis in riverine buffalo (Bubalus bubalis). Asian Australas. Journal of Animal Science. 2013; 26(6):788-794.
- IDF. Bovine Mastitis: Definition and guidelines for diagnosis. Bulletin of the International Dairy Federation, No. 211, Belgium. 1987.
- Ozenc E, Vural MR, Sekeri E, Ucar M. An evaluation of SCM during lactation in Anatolian buffaloes. Turkish Journal of Veterinary and Animal Science. 2008; 32:359-368.
- Quinn PJ, Carter ME, Markey BK, Carter GR. Clinical Veterinary Microbiology. Mosby- Year Book Europe, London, 1994.
- Schalm, OW, Carroll EJ, Jaire NC. Bovine mastitis. Lea and Febiger, Philadelphia, PA. 1971; pp. 80-89.
- Sheffield LG. Mastitis increases growth factor messenger ribonucleic acid in bovine mammary glands. Journal of Dairy Science. 1997; 80:2020‒2024.
- Sudhan NA, Sharma N. Mastitis-An important production disease of dairy animals. SMVS’ DAIRY YEAR BOOK, 2010;72-88.
Investigation of Epidermal Growth Factor (EGF) Levels in Milk of Anatolian Buffaloes with Subclinical Mastitis
Abstract
Subclinical mastitis (SCM)
is a serious disease of farm animals. There are many studies on SCM that aim to
make the diagnosis practical, economical and reliable. It was the aim of the
present study comparison of epidermal growth factors (EGF) ratios in animals
with and without subclinical mastitis as an indictor of SCM in Anatolian water
buffaloes. A study was carried out in a total of on
35 milk samples from 20 healthy and 15 mastitic mammary quarters of clinical
healthy and nonpregnant buffaloes. Samples were examined
using California mastitis test (CMT) as cow side test to detect of SCM before
being transferred to the laboratory. Microbiological culture was also performed
to determine bacterial contamination. Level of EGF and somatic cell count (SCC)
was determined in all samples. As a result of the comparison of the EGF
concentration between the SCM (+) and SCM (-) groups, the SCM (+) group was
found to be higher than the SCM (-) group. EGF tests may be a useful tool in
determining subclinical mastitis.
References
- Bradley AJ. Bovine mastitis: An evolving disease. The Veterinary Journal. 2002; 164:117-126.
- Dallard BE, Ruffino V, Heffel S, Calvinho LF. Effect of a biological response modifier on expression of growth factors and cellular proliferation at drying off. . Journal of Dairy Science. 2007; 90:2229-2240.
- Ergun Y, Aslantas O, Kirecci E, Sarıbay MK, Ates AT, Ulku A, Demir C. Prevalence and etiology of SCM in awassi dairy ewes in southern Turkey. Turkish Journal of Veterinary and Animal Science. 2009; 33:477-483.
- Gabadage, K., Chirino-Trejo, M., Campbell, J., Luby, C. Efficacy of recombinant bovine epidermal growth factor in the treatment of experimental subclinical Staphylococcus aureus mastitis in a ewe model. Veterinary Record Open. 2017; 4(1), e000179.
- Guha A, Guha R, Gera S. Comparison of α1-antitrypsin, α1-acid glycoprotein fibrinogen and Nox as indicator of subclinical mastitis in riverine buffalo (Bubalus bubalis). Asian Australas. Journal of Animal Science. 2013; 26(6):788-794.
- IDF. Bovine Mastitis: Definition and guidelines for diagnosis. Bulletin of the International Dairy Federation, No. 211, Belgium. 1987.
- Ozenc E, Vural MR, Sekeri E, Ucar M. An evaluation of SCM during lactation in Anatolian buffaloes. Turkish Journal of Veterinary and Animal Science. 2008; 32:359-368.
- Quinn PJ, Carter ME, Markey BK, Carter GR. Clinical Veterinary Microbiology. Mosby- Year Book Europe, London, 1994.
- Schalm, OW, Carroll EJ, Jaire NC. Bovine mastitis. Lea and Febiger, Philadelphia, PA. 1971; pp. 80-89.
- Sheffield LG. Mastitis increases growth factor messenger ribonucleic acid in bovine mammary glands. Journal of Dairy Science. 1997; 80:2020‒2024.
- Sudhan NA, Sharma N. Mastitis-An important production disease of dairy animals. SMVS’ DAIRY YEAR BOOK, 2010;72-88.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | September 30, 2019 |
| Acceptance Date | July 16, 2019 |
| Published in Issue | Year 2019 Volume: 12 Issue: 3 |
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