Research Article
Year 2018,
Volume: 11 Issue: 4, 479 - 490, 15.12.2018
Abstract
The aim of the
present study was to evaluate the protective effect of taurine on acrylamide-induced oxidative stress in rats.
Totally, 35 Wistar albino male rats were fed standard rodent diet and divided
into 5 equal groups. I. Group: control group, II. Group: only 15 mg/kg acrylamide
treated by gastric gavage for 60 days.
III., IV., and V. group received 15 mg/kg acrylamide with taurine at dose of 50, 100 and 200 mg/kg/day by gastric
gavage. Treatment with acrylamide significantly increased malondialdehyde
levels and decreased glutathione levels in blood and tissues of rats.
Additionally, acrylamide treatment decreased superoxide dismutase and catalase
activities in erythrocyte and tissues of rats. However, treatment of taurine
inhibited acrylamide-induced oxidative stress, lipid peroxidation, and enhanced
the antioxidant enzymes activities. Besides, mRNA expression levels of IFN-γ,
IL-1β, TNF-α, and NFĸB in liver and brain of acrylamide exposed rats were
decreased with taurine treatment. Moreover, taurine exhibited protective action
against the acrylamide-induced histopathological changes in tissues. In conclusion, it was
determined that taurine in a dose-dependent
manner has a protective effect against acrylamide induced oxidative stress by
increasing antioxidant defense mechanism in rats.
References
- Abdel-Daim MM, Abd Eldaim MA, Hassan AG. Trigonella foenum-graecum ameliorates acrylamide-induced toxicity in rats: roles of oxidative stress, proinflammatory cytokines, and DNA damage. Biochem Cell Biol. 2014; 93 (3): 192–198.
- Acaroz U, Ince S, Arslan-Acaroz D, Gurler Z, Kucukkurt I, Demirel HH., Arslan HO, Varol N, Zhu, K. The ameliorative effects of boron against acrylamide-induced oxidative stress, inflammatory response, and metabolic changes in rats. Food Chem Toxicol. 2018; 118:745-752.
- Aebi H. Catalase in vitro, in: U. Bergmeyer (Ed.), Methods of enzymatic analysis. Academic Press, New York and London. 1974; 673-677.
- Allam A, El-Ghareeb AA, Abdul-Hamid M, Baikry A, Sabri MI. Prenatal and perinatal acrylamide disrupts the development of cerebellum in rat: Biochemical and morphological studies. Toxicol Indust Health. 2011; 27(4): 291-306.
- Alturfan AA, Tozan-Beceren A, Şehirli AÖ, Demiralp E, Şener G, Omurtag GZ. Resveratrol ameliorates oxidative DNA damage and protects against acrylamide- induced oxidative stress in rats. Mol Biol Rep. 2012; 39 (4): 4589–4596.
- Amrein TM, Andres L, Schönbachler B, Conde-Petit B, Escher F, Amado R. Acrylamide in almond products. Europ Food Res Technol. 2005; 221: 14-18.
- Becalski A, Lau BP, Lewis D, Seaman SW. Acrylamide in foods; occurrence, source. Los Angeles CA. AOAC. Annual Meeting and EXPO, 2002; 22–26.
- Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1993; 61: 882-888.
- Bouckenooghe T, Remacle C, Reusens B. Is taurine a functional nutrient? Curr Opin Clin Nutr Metab Care. 2006; 9(6): 728 - 733.
- Cai Q, Rahn Ro, Zhang R. Dietary flavonoids, quercetin, luteolin and genistein, reduce oxidative DNA damage and lipid peroxidation and quench free radicals. Cancer Lett. 1997; 119(1): 99-107.
- Camacho L, Latendresse JR, Muskhelıshvılı L, Patton R, Bowyer JF, Thomas M, Doerge DR. Effects of acrylamide exposure on serum hormones, gene expression, cell proliferation, and histopathology in male reproductive tissues of Fischer 344 rats. Toxicol Lett. 2012; 211: 135-143.
- Claeys WL, De Vleeschouwer K, Hendrickx ME. Quantifying formation of carcinogens during food processing: acrylamide. Trends Food Sci Tech. 2005; 16:181-193.
- Das J, Sil PC. Taurine ameliorates alloxan-induced diabetic renal injury, oxidative stress-related signaling pathways and apoptosis in rats. Amino Acids. 2012; 43: 1509-1523.
- Das, J, Ghosh, J, Manna P, Sinha M, Sil PC. Taurine protects rat testes against NaAsO2-induced oxidative stress and apoptosis via mitochondrial dependent and independent pathways. Toxicol Lett. 2009; 187(3): 201-210.
- Di Monte DA, Chan P, Sandy MS. Glutathione in Parkinson’s disease: a link between oxidative stress and mitochondrial damage? Ann Neurol. 1992; 32: 111-115.
- Doğan İS, Meral R. Gıdalarda Akrilamid ve Önemi. Türkiye 9. Gıda Kongresi; 24-26 Mayıs 2006, Bolu.
- Drabkin DL, Austin JH. Spectrophotometric studies. II. Preparations from washed blood cells; nitric oxide hemoglobin and sulfhemoglobin. J Biol Chem. 1935;112: 51-65.
- Draper HH, Hardley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990; 186: 421-431.
- Fiedan IO, Ahmed EA, Omar HEDM. Acrylamide induced testicular toxicity in rats: protective effect of garlic oil. Biomarkers. 2015; 1: 1-5.
- Engin A, Bozkurt BS, Altan N, Memiş L, Bukan N. Nitric oxide-mediated liver injury in presence of experimental bile duct obstruction. World J Surg. 2003; 27(3): 253-255.
- Hamdy SM, Bakeer HM, Eskander EF, Sayed ON. Effect of acrylamide on some hormones and endocrine tissues in male rats. Human Experiment Toxicol. 2012; 31 (5): 483-491.
- Hammad AY, Osman ME, Abdelgadir WS. Histopathological Assessment and hematotoxicity of Dietary acrylamide on Wistar rats. Int JLife Sci. 2013; 7(1): 21-25.
- Ince S, Arslan-Acaroz D, Demirel HH, Varol N, Ozyurek HA, Zemheri F, Kucukkurt I. Taurine alleviates malathion induced lipid peroxidation, oxidative stress, and proinflammatory cytokine gene expressions in rats. Biomed Pharmacother. 2017; 96: 263-268.
- Jong CJ, Azuma J, Schaffer S. Mechanism underlying the antioxidant activity of taurine: prevention of mitochondrial oxidant production. Amino Acids. 2012; 42: 2223-32.
- Kucukkurt I, Ince S, Fidan AF, Ozdemir A. The effects of dietary supplementation of different amount of Yucca schidigera powder (Sarsaponin 30®) on blood and tissue antioxidant defense systems and lipid peroxidation in rats. J Anim Vet Advan. 2008; 7: 1413-1417.
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193: 265-75.
- Luck H. Catalase. in: Bergmeyer, H.U. (Ed.), Methods in Analysis. Academy Press, London. 1955.
- Mercan U. Toksikolojide Serbest Radikallerin Önemi. Y Y Üniv Vet Fak Derg. 2004; 15(1-2): 91-96.
- Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351-358.
- Patel PG, Kapadiya KB, Patel BJ. Protective effect of vitamin E on biochemistry, oxidative stress and histopathological alterations induced by acrylamide in wistar rats (Rattus norvegicus). Vet Sci Res J. 2015; 6(1): 16-22.
- Perfeito R, Cunha-Oliveira T, Cristina Rego A. Revisiting oxidative stress and mitochondrial dysfunction in the pathogenesis of Parkinson disease-resemblance to the effect of amphetamine drugs of abuse. Free Rad Biol Med. 2012; 53: 1791–1806.
- Rice JM. The carcinogenicity of acrylamide. Mutation Res. 2005; 580: 3–20.
- Robert GT, Michael LG, Christopher R, Kirman M, Leigh Carson, Lisa MS. Estimation of safe dietary intake levels of acrylamide for humans. Food Chem Toxicol. 2010; 48: 658–667.
- Saulo AA. Acrylamide in foods. Food Safety Technol. 2003: 1-2.
- Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988; 34: 497-500.
- Totani N, Yawata M, Ojiri Y, Fujioka Y. Effects of trace acrylamide intake in Wistar rats. J Oleo Sci. 2007; 56 (9): 501-6.
- Tyl R, Crump K. Acrylamide in Food. Food Standards Agency, 2003; 5: 215–222.
- Winterbourn CC, Hawkins RE, Brain M, Carrell RW. The estimation of red cell superoxide activity. J Lab Clin Med. 1975; 55: 337-341.
- Wojtecka-Lukasik E, Czuprynska K, Maslinska D, Gajewski M, Gujski M, Maslinski S. Taurine-chloramine is a potent antiinflammatory substance. Inflam Res. 2006; 55(1): 17-18.
- Yousef MI, El-Demerdash FM. Acrylamide-induced oxidative stres and biochemical perturbations in rats. Toxicology. 2006; 219: 133-141.
- Yuan LQ, Liu W, Cui RR, Wang D, Meng JC, Xie H, Liao YE. Taurine inhibits osteoclastogenesis through the taurine transporter. Amino Acids, 2010; 39(1): 89-99.
- Zhang JX, Yue WB, Ren YS, Zhang CX. Enhanced fat consumption potentiates acrylamide-induced oxidative stress in epididymis and epididymal sperm and effect spermatogenesis in mice. Toxicol Mech Methods. 2010; 20 (2): 75-81.
- Zhang L, Zhang H, Mıao Y, Wu S, Ye H, Yuan Y. Protective effect of allicin againts acrylamide-induced hepatocyte damage in vitro and in vivo. Food Chem Toxicol. 2012; 50: 3306-3312.
- Zhang Y, Zhang G, Zhang Y. Occurrence and analytical methods o f acrylamide in heat-treated foods. Review and recent developments. J Chrom A. 2005; 1075: 1-21.
- Zhang L, Wang E, Chen F, Yan H, Yuan Y. Potential protective effects of oral administration of allicin on acrylamide-induced toxicity in male mice. Food Funct. 2013; 4: 1229.
- Zulli A. Taurine in cardiovascular disease. Curr Opin Clin Nutr Metab Care. 2011; 14: 57-60.
Year 2018,
Volume: 11 Issue: 4, 479 - 490, 15.12.2018
Abstract
Bu çalışmada, sıçanlarda akrilamid ile oluşturulan oksidatif strese
karşı antioksidan ajan olan taurinin koruyucu etkisinin araştırılması
amaçlandı. Toplam 35 Wistar erkek sıçan standart rodent yemiyle beslendi ve 5
eşit gruba ayrıldı. I. Grup: kontrol grubu, II. Grup: sadece 15 mg/kg
akrilamid 60 gün boyunca gastrik gavaj yolu ile verildi. III., IV. ve V.
gruplara sırasıyla akrilamid (15 mg/kg) ve taurin 50, 100 ve 200 mg/kg/gün
olacak şekilde ağızdan gastrik gavaj ile verildi. Akrilamid uygulaması
sıçanların kan ve dokularında malondialdehid seviyelerinde önemli bir artışa ve
glutatyon seviyelerinde azalmaya neden oldu. Buna ilave olarak, akrilamid
uygulaması ile süperoksid dismutaz ve katalaz aktivitelerinin sıçan eritrosit
ve dokularında azaldığı belirlendi. Taurin uygulamasının akrilamid ile
indüklenen oksidatif stres ve lipid peroksidasyonu azaltarak vücudun antioksidan savunma
sistemini iyileştirdiği belirlendi. Bunun yanı sıra, taurin uygulaması ile akrilamid
verilen sıçanların karaciğer ve beyin dokularındaki IFN-γ, IL-1β, TNF-α ve NFĸB
mRNA ekspresyon düzeylerinin azaldığı gözlendi. Ayrıca, taurin
dokularda akrilamid ile indüklenen oksidatif stres sonucu oluşan histopatolojik
değişikliklere karşı koruyucu etki gösterdi. Sonuçta doza bağlı olarak taurinin
sıçanlarda antioksidan savunma mekanizmasını artırarak akrilamidle indüklenen oksidatif strese karşı koruyucu etki
gösterdiği belirlendi.
References
- Abdel-Daim MM, Abd Eldaim MA, Hassan AG. Trigonella foenum-graecum ameliorates acrylamide-induced toxicity in rats: roles of oxidative stress, proinflammatory cytokines, and DNA damage. Biochem Cell Biol. 2014; 93 (3): 192–198.
- Acaroz U, Ince S, Arslan-Acaroz D, Gurler Z, Kucukkurt I, Demirel HH., Arslan HO, Varol N, Zhu, K. The ameliorative effects of boron against acrylamide-induced oxidative stress, inflammatory response, and metabolic changes in rats. Food Chem Toxicol. 2018; 118:745-752.
- Aebi H. Catalase in vitro, in: U. Bergmeyer (Ed.), Methods of enzymatic analysis. Academic Press, New York and London. 1974; 673-677.
- Allam A, El-Ghareeb AA, Abdul-Hamid M, Baikry A, Sabri MI. Prenatal and perinatal acrylamide disrupts the development of cerebellum in rat: Biochemical and morphological studies. Toxicol Indust Health. 2011; 27(4): 291-306.
- Alturfan AA, Tozan-Beceren A, Şehirli AÖ, Demiralp E, Şener G, Omurtag GZ. Resveratrol ameliorates oxidative DNA damage and protects against acrylamide- induced oxidative stress in rats. Mol Biol Rep. 2012; 39 (4): 4589–4596.
- Amrein TM, Andres L, Schönbachler B, Conde-Petit B, Escher F, Amado R. Acrylamide in almond products. Europ Food Res Technol. 2005; 221: 14-18.
- Becalski A, Lau BP, Lewis D, Seaman SW. Acrylamide in foods; occurrence, source. Los Angeles CA. AOAC. Annual Meeting and EXPO, 2002; 22–26.
- Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1993; 61: 882-888.
- Bouckenooghe T, Remacle C, Reusens B. Is taurine a functional nutrient? Curr Opin Clin Nutr Metab Care. 2006; 9(6): 728 - 733.
- Cai Q, Rahn Ro, Zhang R. Dietary flavonoids, quercetin, luteolin and genistein, reduce oxidative DNA damage and lipid peroxidation and quench free radicals. Cancer Lett. 1997; 119(1): 99-107.
- Camacho L, Latendresse JR, Muskhelıshvılı L, Patton R, Bowyer JF, Thomas M, Doerge DR. Effects of acrylamide exposure on serum hormones, gene expression, cell proliferation, and histopathology in male reproductive tissues of Fischer 344 rats. Toxicol Lett. 2012; 211: 135-143.
- Claeys WL, De Vleeschouwer K, Hendrickx ME. Quantifying formation of carcinogens during food processing: acrylamide. Trends Food Sci Tech. 2005; 16:181-193.
- Das J, Sil PC. Taurine ameliorates alloxan-induced diabetic renal injury, oxidative stress-related signaling pathways and apoptosis in rats. Amino Acids. 2012; 43: 1509-1523.
- Das, J, Ghosh, J, Manna P, Sinha M, Sil PC. Taurine protects rat testes against NaAsO2-induced oxidative stress and apoptosis via mitochondrial dependent and independent pathways. Toxicol Lett. 2009; 187(3): 201-210.
- Di Monte DA, Chan P, Sandy MS. Glutathione in Parkinson’s disease: a link between oxidative stress and mitochondrial damage? Ann Neurol. 1992; 32: 111-115.
- Doğan İS, Meral R. Gıdalarda Akrilamid ve Önemi. Türkiye 9. Gıda Kongresi; 24-26 Mayıs 2006, Bolu.
- Drabkin DL, Austin JH. Spectrophotometric studies. II. Preparations from washed blood cells; nitric oxide hemoglobin and sulfhemoglobin. J Biol Chem. 1935;112: 51-65.
- Draper HH, Hardley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990; 186: 421-431.
- Fiedan IO, Ahmed EA, Omar HEDM. Acrylamide induced testicular toxicity in rats: protective effect of garlic oil. Biomarkers. 2015; 1: 1-5.
- Engin A, Bozkurt BS, Altan N, Memiş L, Bukan N. Nitric oxide-mediated liver injury in presence of experimental bile duct obstruction. World J Surg. 2003; 27(3): 253-255.
- Hamdy SM, Bakeer HM, Eskander EF, Sayed ON. Effect of acrylamide on some hormones and endocrine tissues in male rats. Human Experiment Toxicol. 2012; 31 (5): 483-491.
- Hammad AY, Osman ME, Abdelgadir WS. Histopathological Assessment and hematotoxicity of Dietary acrylamide on Wistar rats. Int JLife Sci. 2013; 7(1): 21-25.
- Ince S, Arslan-Acaroz D, Demirel HH, Varol N, Ozyurek HA, Zemheri F, Kucukkurt I. Taurine alleviates malathion induced lipid peroxidation, oxidative stress, and proinflammatory cytokine gene expressions in rats. Biomed Pharmacother. 2017; 96: 263-268.
- Jong CJ, Azuma J, Schaffer S. Mechanism underlying the antioxidant activity of taurine: prevention of mitochondrial oxidant production. Amino Acids. 2012; 42: 2223-32.
- Kucukkurt I, Ince S, Fidan AF, Ozdemir A. The effects of dietary supplementation of different amount of Yucca schidigera powder (Sarsaponin 30®) on blood and tissue antioxidant defense systems and lipid peroxidation in rats. J Anim Vet Advan. 2008; 7: 1413-1417.
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193: 265-75.
- Luck H. Catalase. in: Bergmeyer, H.U. (Ed.), Methods in Analysis. Academy Press, London. 1955.
- Mercan U. Toksikolojide Serbest Radikallerin Önemi. Y Y Üniv Vet Fak Derg. 2004; 15(1-2): 91-96.
- Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351-358.
- Patel PG, Kapadiya KB, Patel BJ. Protective effect of vitamin E on biochemistry, oxidative stress and histopathological alterations induced by acrylamide in wistar rats (Rattus norvegicus). Vet Sci Res J. 2015; 6(1): 16-22.
- Perfeito R, Cunha-Oliveira T, Cristina Rego A. Revisiting oxidative stress and mitochondrial dysfunction in the pathogenesis of Parkinson disease-resemblance to the effect of amphetamine drugs of abuse. Free Rad Biol Med. 2012; 53: 1791–1806.
- Rice JM. The carcinogenicity of acrylamide. Mutation Res. 2005; 580: 3–20.
- Robert GT, Michael LG, Christopher R, Kirman M, Leigh Carson, Lisa MS. Estimation of safe dietary intake levels of acrylamide for humans. Food Chem Toxicol. 2010; 48: 658–667.
- Saulo AA. Acrylamide in foods. Food Safety Technol. 2003: 1-2.
- Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988; 34: 497-500.
- Totani N, Yawata M, Ojiri Y, Fujioka Y. Effects of trace acrylamide intake in Wistar rats. J Oleo Sci. 2007; 56 (9): 501-6.
- Tyl R, Crump K. Acrylamide in Food. Food Standards Agency, 2003; 5: 215–222.
- Winterbourn CC, Hawkins RE, Brain M, Carrell RW. The estimation of red cell superoxide activity. J Lab Clin Med. 1975; 55: 337-341.
- Wojtecka-Lukasik E, Czuprynska K, Maslinska D, Gajewski M, Gujski M, Maslinski S. Taurine-chloramine is a potent antiinflammatory substance. Inflam Res. 2006; 55(1): 17-18.
- Yousef MI, El-Demerdash FM. Acrylamide-induced oxidative stres and biochemical perturbations in rats. Toxicology. 2006; 219: 133-141.
- Yuan LQ, Liu W, Cui RR, Wang D, Meng JC, Xie H, Liao YE. Taurine inhibits osteoclastogenesis through the taurine transporter. Amino Acids, 2010; 39(1): 89-99.
- Zhang JX, Yue WB, Ren YS, Zhang CX. Enhanced fat consumption potentiates acrylamide-induced oxidative stress in epididymis and epididymal sperm and effect spermatogenesis in mice. Toxicol Mech Methods. 2010; 20 (2): 75-81.
- Zhang L, Zhang H, Mıao Y, Wu S, Ye H, Yuan Y. Protective effect of allicin againts acrylamide-induced hepatocyte damage in vitro and in vivo. Food Chem Toxicol. 2012; 50: 3306-3312.
- Zhang Y, Zhang G, Zhang Y. Occurrence and analytical methods o f acrylamide in heat-treated foods. Review and recent developments. J Chrom A. 2005; 1075: 1-21.
- Zhang L, Wang E, Chen F, Yan H, Yuan Y. Potential protective effects of oral administration of allicin on acrylamide-induced toxicity in male mice. Food Funct. 2013; 4: 1229.
- Zulli A. Taurine in cardiovascular disease. Curr Opin Clin Nutr Metab Care. 2011; 14: 57-60.
There are 46 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | December 15, 2018 |
| Acceptance Date | October 16, 2018 |
| Published in Issue | Year 2018 Volume: 11 Issue: 4 |
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