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The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats

Year 2015, Volume: 8 Issue: 1, 45 - 50, 01.06.2015

Abstract

Bu çalışma Wistar Albino rat kullanılarak 15 gün boyunca gerçekleştirildi (n=7). 1)Kontrol: Fizyolojik salin gastrik gavaj ile verildi. 2) CsA: CsA (15 mg/kg/gün) subkutan (sc) olarak uygulandı. 3) CsA+SO grup: CsA (15 mg/kg/day; sc) ve SO (1 ml/kg, gavaj) olarak verildi. 4) CsA+O-3: CsA (15 mg/kg/gün; sc) ve O-3 (100 mg/kg, gavaj) olarak verildi. 5) CsA+SO+O-3: CsA (15 mg/kg/gün; sc), SO (1 ml/kg, gavaj) ve O-3 (100 mg/kg, gavaj) eş zamanlı olarak verildi. 6) SO: sadece SO (1 ml/kg; gavaj) ve 7) O-3: sadece O-3 (100 mg/kg, gavaj). Son uygulamalardan 24 saat sonra ratlar sakrifiye edildi. Biyokimyasal analizler için kan, karaciğer ve böbrek dokuları toplandı. Tüm gruplar arasında NOx bakımında bir fark belirlenmedi. CsA gruba göre diğer tüm gruplarda IL-1α, IL-1β seviyesindeki değişimler önemsizdi. Buna karşın IFN-γ düzeyleri SO ve O-3 hariç diğer tüm gruplarda arttı. Kontrol gruba göre CsA grubunda MDA, GSH, SOD ve CAT düzeylerindeki değişimler hem karaciğer hem de böbrek dokusunda önemsizdi. CsA grubuna göre CsA+O-3 grubunda böbrek dokusu MDA, GSH ve SOD düzeyleri yüksek bulunurken, buna karşın karaciğerdeki değişimler önemsizdi. Bu bulgulara göre, CsA ile birlikte SO ve O-3'ün kullanımı böbreklerde CsA'nın indüklediği oksidatif stresin önlenmesinde yeni bir yaklaşım olabilir.

References

  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione.Journal of Laboratory and Clinical Medicine. 1963; 61: 882-888.
  • Böhmer AE, Ribeiro Corrêa A.M, de Souza DG, Knorr L, Hansel G, Corbellini LG, Driemeier D, Portela LV, Souza DO. Long-term cyclosporine treatment: Evaluation of serum biochemical parameters and histopathological alterations in Wistar rats. Experimental and Toxicologic Pathology. 2011; 63: 119-123.
  • Budowski P, Markley KS. The chemical and physiological properties of sesame oil. Chemical Reviews. 1951; 48: 125-151.
  • Cauwels A, Brouckaert P. Survival of TNF toxicity: dependence on cascades and NO. Archives of Biochemistry and Biophysics. 2007; 462: 132-139.
  • De Hornedo JP, De Arriba G, Fernandez MC, Benito S, Cid TP. Cyclosporin A causes oxidative stress and mitochondrial dysfunction in renal tubular cells. Nefrologia. 2007; 27: 565-573.
  • Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology. 1990; 186: 421-431.
  • Goksu Erol AY, Avcı G, Sevimli A, Ulutas E, Ozdemir M. The protective effects of omega 3 fatty acids and sesame oil against cyclosporine A-induced nephrotoxicity. Drug and Chemical Toxicology. 2013; 36: 241-248.
  • Göksu Erol AY, Bülbül A, Avcı G, Özdemir M, Akkaya Ö. The Protective Effects of Omega-3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Liver Apoptosis. Journal of Academic Research in Medicine. 2011; 1: 8-11.
  • Haberland A, Henke W, Grune T, Siems W, Jung K, Schimke I. Differential response of oxygen radical metabolism in rat heart, liver and kidney to cyclosporine A treatment. Inflammation Research. 1997; 46: 452-454.
  • Horn DL, Opal SM, Lomastro E. Antibiotics, cytokines, and endotoxin: A complex and evolving relationship in gram-negative sepsis. Scandinavian Journal of Infectious Diseases. Supplementum. 1996; 101: 9-13.
  • Hortelano S, Castilla M, Torres AM, Tejedor A, Bosc’a L. Potentiation by nitric oxide of cyclosporin A and FK506-induced apoptosis in renal proximal tubule cells. Journal of the American Society of Nephrology. 2000; 11: 2315–2323.
  • Hsu DZ, Chen KT, Lin TH, Li YH, Liu MY. Sesame oil attenuates cisplatin induced hepatic and renal injuries by inhibiting nitric oxide-associated lipid peroxidation in mice. Shock. 2007; 27: 199-204.
  • Hsu DZ, Liu MY. Sesame oil protects against lipopolysaccharide-stimulated oxidative stress in rats. Critical Care Medicine. 2004; 32: 227-231.
  • Kris-Etherton P, Eckel RH, Howard BV, Jeor S St, Bazzare TL. AHA Science Advisory: Lyon Diet Heart Study. Benefits of a Mediterranean-style, National Cholesterol Education Program/American Heart Association Step I Dietary Pattern on Cardiovascular Disease. Circulation. 2001; 103: 1823 -1825.
  • Leaf A, Weber PC.. Cardiovascular effects of n-3 fatty acids. The New England Journal of Medicine. 1988; 318: 549-557.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry. 1951; 193: 265-275.
  • Luck H. Catalase, In: Methods in Analysis, Bergmeyer H.U. (ed.), Academy Press, London. 1965; pp. 855-884.
  • Mariee AD, Abd-Ellah MF. Protective effect of docosahexaenoic acid against cyclosporine A-induced nephrotoxicity in rats: a possible mechanism of action. Renal Failure. 2011; 33: 66-71.
  • Matsuda S, Koyasu S. Mechanisms of action of cyclosporine. Immunopharmacology. 2000; 47: 119-125.
  • Mylonas C, Kouretas D. Lipid peroxidation and tissue damage. In Vivo (Athens, Greece). 1999; 13: 295-309.
  • Namiki M. Nutraceutical functions of sesame: a review. Critical Reviews in Food Science and Nutrition. 2007; 47: 651-673.
  • Noronha IL, Niemir Z, Stein H, Waldher R. Cytokines and growth factors in renal disease. Nephrology Dialysis Transplantation. 1995; 10: 775-786.
  • Oriji GK. Nitric oxide in cyclosporine A-induced hypertension: endothelin receptors gene expression. Prostaglandins, Leukotriens and Essential Fatty Acids. 1999; 61: 41-44.
  • Ponticelli C. Cyclosporine: from renal transplantation to autoimmune diseases. Annals of the New York Academy of Science. 2005; 1051: 551-558.
  • Shidfar F, Keshavarz A, Hosseyni S, Ameri A, Yarahmadi S. Effects of omega-3 fatty acid supplements on serum lipids, apolipoproteins and malondialdehyde in type 2 diabetes patients. Eastern Mediterranean Health Journal. 2008; 14: 305-313.
  • Simopoulos AP. Omega-3 fatty acids and athletics. Current Sports Medicine Reports. 2007; 6: 230-236.
  • Stähelin H. Cyclosporin A:Historical background. Progressin Allergy. 1986; 38: 19-27.
  • Sun Y, Oberley LW, Li YA. Simple method for clinical assay of superoxide dismutase. Clinical Chemistry. 1988; 34: 497-500.
  • Tedesco D, Haragsim L. Cyclosporine: A Review. Journal of Transplantation, Article ID 230386, 7 pages, doi:10.1155/2012/230386
  • Yazar E, Er A, Uney K, Bulbul A, Avci GE, Elmas M, Tras B. Effects of drugs used in endotoxic shock on oxidative stress and organ damage markers. Free Radical Research. 2010; 44: 397-402.
  • Zhong Z, Arteel GE, Connor HD, Yin M, Frankenberg MV, Stachlewitz RF, Raleigh JA, Mason RP, Thurman RG. Cyclosporin A increases hypoxia and free radical production in rat kidneys: prevention by dietary glycine. American Journal of Physiology-Renal Physiology. 1998; 275: F595-F604.

The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats

Year 2015, Volume: 8 Issue: 1, 45 - 50, 01.06.2015

Abstract

The purpose of this study was to investigate the effects of omega-3 (O-3) and sesame oil (SO) on cyclosporine-A (CsA) induced immunosuppressive and oxidant effects in rats. The study was performed on seven groups for 15 days using Wistar Albino rats (n=7),1) Control: physiological saline was given by gastric gavage, 2) CsA: CsA was administered (15 mg/kg/day) subcutaneously (sc),3) CsA+SO: CsA (15 mg/kg/day; sc) and SO (1 ml/kg, gavage) were given, 4) CsA+O-3: CsA, (15 mg/kg/day; sc) and O-3 (100 mg/kg, gavage) were administered, 5) CsA+SO+O-3: CsA (15 mg/kg/day; sc), SO (1 ml/kg, gavage) and O-3 (100 mg/kg, gavage) were given at the same time, 6) SO: only SO (1 ml/kg; gavage) 7) O-3: only O-3 (100 mg/kg, gavage). 24 h after the last treatments animals were sacrificed. Blood, livers and kidneys were collected for biochemical analysis. There was no difference among all groups in terms of nitric oxide (NOx). Changes in the IL-1α, IL-1β levels were insignificant all other groups compared to CsA group. Nonetheless IFN-γ levels were increased in all groups except SO, O-3 groups. Changes in the level of MDA, GSH, SOD and CAT in both kidney and liver tissues were negligible in the CsA group compared to control group. MDA, GSH and SOD levels in the kidney tissue were determined higher in the CsA groups than CsA+O-3 group, nevertheless changes in the liver were insignificant. Given these findings, use of SO and O-3 with CsA may be a new approach for preventing CsA- induced oxidative stress in kidneys.

References

  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione.Journal of Laboratory and Clinical Medicine. 1963; 61: 882-888.
  • Böhmer AE, Ribeiro Corrêa A.M, de Souza DG, Knorr L, Hansel G, Corbellini LG, Driemeier D, Portela LV, Souza DO. Long-term cyclosporine treatment: Evaluation of serum biochemical parameters and histopathological alterations in Wistar rats. Experimental and Toxicologic Pathology. 2011; 63: 119-123.
  • Budowski P, Markley KS. The chemical and physiological properties of sesame oil. Chemical Reviews. 1951; 48: 125-151.
  • Cauwels A, Brouckaert P. Survival of TNF toxicity: dependence on cascades and NO. Archives of Biochemistry and Biophysics. 2007; 462: 132-139.
  • De Hornedo JP, De Arriba G, Fernandez MC, Benito S, Cid TP. Cyclosporin A causes oxidative stress and mitochondrial dysfunction in renal tubular cells. Nefrologia. 2007; 27: 565-573.
  • Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology. 1990; 186: 421-431.
  • Goksu Erol AY, Avcı G, Sevimli A, Ulutas E, Ozdemir M. The protective effects of omega 3 fatty acids and sesame oil against cyclosporine A-induced nephrotoxicity. Drug and Chemical Toxicology. 2013; 36: 241-248.
  • Göksu Erol AY, Bülbül A, Avcı G, Özdemir M, Akkaya Ö. The Protective Effects of Omega-3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Liver Apoptosis. Journal of Academic Research in Medicine. 2011; 1: 8-11.
  • Haberland A, Henke W, Grune T, Siems W, Jung K, Schimke I. Differential response of oxygen radical metabolism in rat heart, liver and kidney to cyclosporine A treatment. Inflammation Research. 1997; 46: 452-454.
  • Horn DL, Opal SM, Lomastro E. Antibiotics, cytokines, and endotoxin: A complex and evolving relationship in gram-negative sepsis. Scandinavian Journal of Infectious Diseases. Supplementum. 1996; 101: 9-13.
  • Hortelano S, Castilla M, Torres AM, Tejedor A, Bosc’a L. Potentiation by nitric oxide of cyclosporin A and FK506-induced apoptosis in renal proximal tubule cells. Journal of the American Society of Nephrology. 2000; 11: 2315–2323.
  • Hsu DZ, Chen KT, Lin TH, Li YH, Liu MY. Sesame oil attenuates cisplatin induced hepatic and renal injuries by inhibiting nitric oxide-associated lipid peroxidation in mice. Shock. 2007; 27: 199-204.
  • Hsu DZ, Liu MY. Sesame oil protects against lipopolysaccharide-stimulated oxidative stress in rats. Critical Care Medicine. 2004; 32: 227-231.
  • Kris-Etherton P, Eckel RH, Howard BV, Jeor S St, Bazzare TL. AHA Science Advisory: Lyon Diet Heart Study. Benefits of a Mediterranean-style, National Cholesterol Education Program/American Heart Association Step I Dietary Pattern on Cardiovascular Disease. Circulation. 2001; 103: 1823 -1825.
  • Leaf A, Weber PC.. Cardiovascular effects of n-3 fatty acids. The New England Journal of Medicine. 1988; 318: 549-557.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry. 1951; 193: 265-275.
  • Luck H. Catalase, In: Methods in Analysis, Bergmeyer H.U. (ed.), Academy Press, London. 1965; pp. 855-884.
  • Mariee AD, Abd-Ellah MF. Protective effect of docosahexaenoic acid against cyclosporine A-induced nephrotoxicity in rats: a possible mechanism of action. Renal Failure. 2011; 33: 66-71.
  • Matsuda S, Koyasu S. Mechanisms of action of cyclosporine. Immunopharmacology. 2000; 47: 119-125.
  • Mylonas C, Kouretas D. Lipid peroxidation and tissue damage. In Vivo (Athens, Greece). 1999; 13: 295-309.
  • Namiki M. Nutraceutical functions of sesame: a review. Critical Reviews in Food Science and Nutrition. 2007; 47: 651-673.
  • Noronha IL, Niemir Z, Stein H, Waldher R. Cytokines and growth factors in renal disease. Nephrology Dialysis Transplantation. 1995; 10: 775-786.
  • Oriji GK. Nitric oxide in cyclosporine A-induced hypertension: endothelin receptors gene expression. Prostaglandins, Leukotriens and Essential Fatty Acids. 1999; 61: 41-44.
  • Ponticelli C. Cyclosporine: from renal transplantation to autoimmune diseases. Annals of the New York Academy of Science. 2005; 1051: 551-558.
  • Shidfar F, Keshavarz A, Hosseyni S, Ameri A, Yarahmadi S. Effects of omega-3 fatty acid supplements on serum lipids, apolipoproteins and malondialdehyde in type 2 diabetes patients. Eastern Mediterranean Health Journal. 2008; 14: 305-313.
  • Simopoulos AP. Omega-3 fatty acids and athletics. Current Sports Medicine Reports. 2007; 6: 230-236.
  • Stähelin H. Cyclosporin A:Historical background. Progressin Allergy. 1986; 38: 19-27.
  • Sun Y, Oberley LW, Li YA. Simple method for clinical assay of superoxide dismutase. Clinical Chemistry. 1988; 34: 497-500.
  • Tedesco D, Haragsim L. Cyclosporine: A Review. Journal of Transplantation, Article ID 230386, 7 pages, doi:10.1155/2012/230386
  • Yazar E, Er A, Uney K, Bulbul A, Avci GE, Elmas M, Tras B. Effects of drugs used in endotoxic shock on oxidative stress and organ damage markers. Free Radical Research. 2010; 44: 397-402.
  • Zhong Z, Arteel GE, Connor HD, Yin M, Frankenberg MV, Stachlewitz RF, Raleigh JA, Mason RP, Thurman RG. Cyclosporin A increases hypoxia and free radical production in rat kidneys: prevention by dietary glycine. American Journal of Physiology-Renal Physiology. 1998; 275: F595-F604.
There are 31 citations in total.

Details

Journal Section RESEARCH ARTICLE
Authors

Gülcan Avcı

Aziz Bülbül This is me

Mehmet Özdemir This is me

A.yasemin Göksu Erol This is me

Publication Date June 1, 2015
Acceptance Date February 9, 2015
Published in Issue Year 2015 Volume: 8 Issue: 1

Cite

APA Avcı, G., Bülbül, A., Özdemir, M., Göksu Erol, A. (2015). The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats. Kocatepe Veterinary Journal, 8(1), 45-50.
AMA Avcı G, Bülbül A, Özdemir M, Göksu Erol A. The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats. kvj. January 2015;8(1):45-50.
Chicago Avcı, Gülcan, Aziz Bülbül, Mehmet Özdemir, and A.yasemin Göksu Erol. “The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats”. Kocatepe Veterinary Journal 8, no. 1 (January 2015): 45-50.
EndNote Avcı G, Bülbül A, Özdemir M, Göksu Erol A (January 1, 2015) The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats. Kocatepe Veterinary Journal 8 1 45–50.
IEEE G. Avcı, A. Bülbül, M. Özdemir, and A. Göksu Erol, “The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats”, kvj, vol. 8, no. 1, pp. 45–50, 2015.
ISNAD Avcı, Gülcan et al. “The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats”. Kocatepe Veterinary Journal 8/1 (January 2015), 45-50.
JAMA Avcı G, Bülbül A, Özdemir M, Göksu Erol A. The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats. kvj. 2015;8:45–50.
MLA Avcı, Gülcan et al. “The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats”. Kocatepe Veterinary Journal, vol. 8, no. 1, 2015, pp. 45-50.
Vancouver Avcı G, Bülbül A, Özdemir M, Göksu Erol A. The Investigation of The Effects of Omega 3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Immunosupressive and Oxidant Effects in Rats. kvj. 2015;8(1):45-50.

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