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Yüzme Egzersizi Yaptırılan Farelere Alfa-Lipoik Asit Uygulamasının Lipid Peroksidasyon, Kreatin Kinaz ve Laktat Düzeyleri Üzerine Etkisinin İncelenmesi

Year 2023, Volume: 18 Issue: 1, 102 - 107, 16.03.2023
https://doi.org/10.17517/ksutfd.1080134

Abstract

Amaç: Bu çalışmada, yüzme egzersizi yaptırılan farelere alfa-lipoik asit (ALA) uygulamasının lipit peroksidasyonu, kreatin kinaz ve laktat düzeyleri üzerine etkisi incelendi.
Gereç ve Yöntemler: Çalışmada 20-25 gram (gr) ağırlığında, 30 adet Swiss albino cinsi fare kullanıldı. Fareler kontrol, ALA-50 ve ALA-100 şeklinde her grupta 10 adet olacak şekilde 3 gruba ayrıldı. Kontrol grubuna 10 gün boyunca intraperitoneal (i.p.) izotonik serum uygulandı. ALA gruplarına ise 10 gün boyunca i.p. yolla sırasıyla 50 ve 100 mg/kg konsantrasyonlarda ALA uygulandı. Onuncu günün sonunda fareler yüzme havuzlarına alınarak test edildi. Yüzme testi sonrası farelerin kas ve kan örnekleri alınarak biyokimyasal olarak incelendi.
Bulgular: Elde edilen veriler kontrol grubu ile karşılaştırıldığında ALA uygulanan grupların yüzme süresinde, GSH, GSH-Px ve SOD seviyelerinde artış, CK, LDH ve MDA değerlerinde ise azalma olduğu görüldü.
Sonuç: Sonuç olarak akut tüketici yüzme egzersizi yaptırılan farelerin kas dokusundaki hasarın varlığı biyokimyasal olarak tespit edildi. ALA uygulamalarının tüketici yüzme egzersizinin oluşturduğu oksidatif hasarın engellenmesinde ve kas yorgunluğunun geciktirilmesinde etkili olabileceği kanısına varıldı.

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Thanks

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References

  • Kin A. Step ve Aerobik Dansın Üniversiteli Bayanların Fizyolojik Parametrelerine Etkisinin Karşılaştırılması, Yüksek Lisans Tezi; Ankara: ODTÜ, 1996.
  • Baltacı Kasım A. Çocuklarda yüzme egzersizinin solunum parametrelerine etkisi. Sağlık Bilimleri Enstitüsü, Fizyoloji Anabilim Dalı, Yüksek Lisans Tezi:, Konya: Selçuk Üniverisitesi, 1990.
  • Stanford D, Stanforth PR, Velasquez KS. Aerobic Requirement of bench stepping. Int. J. Sports Med. 1993;14:129-133.
  • Turanlıv AY. Deri Yaşlanmasında Serbest Radikallerin Yeri, 3. Uludağ Dermatokozmetotloji Günleri Sempozyumu, 2005:100-106.
  • Hajam YA, Rani R, Ganie SY, Sheikh TA, Javaid D, Qadri SS et al. Oxidative stress in human pathology and aging: molecular mechanisms and perspectives. Cells. 2022;11(3):552.
  • Barghi N, Bambaeichi E, Rezaei-Tavirani M, Khaledi N. Aerobic exercises ınduce antioxidant pathways activation in rats. Int J Prev Med. 2020;11:144.
  • Öztürkcan S, Kayhan TÇ. Deri yaşlanmasına karşı medikal önlemler, Dermatoz 2010;1:77-82.
  • Lu Y, Wiltshire HD, Baker JS, Wang Q. Effects of high ıntensity exercise on oxidative stress and antioxidant status in untrained humans: A Systematic Review. Biology (Basel). 2021;10(12):1272.
  • Pala R, Beyaz F, Tuzcu M, Er B, Sahin N, Cinar V ve ark. The effects of coenzyme Q10 on oxidative stress and heat shock proteins in rats subjected to acute and chronic exercise. J. Exerc. Nutrition Biochem. 2018;22(3):14-20.
  • Liang JF, Akaike T. Inhibition of nitric oxide synthesis in primary cultured Mouse hepatocytes by α-lipoic acid. Chem. Biol. Interact. 2000, 124:53-60.
  • St Clair D, Zhao Y, Chaiswing L, Oberley T. Modulation of skin tumorigenesis by SOD. Biomed. Pharmacother. 2005;59:209-214.
  • Salehi B, Berkay Yılmaz Y, Antika G, Boyunegmez Tumer T et al. Insights on the Use of α-Lipoic Acid for Therapeutic Purposes. Biomolecules. 2019;9(8):356.
  • Çakatay U. Pro-oxidant actions of α-lipoic acid and dihydrolipoic acid. Med. Hypotheses. 2006,66:110-117.
  • Ji LL, Yeo D. Oxidative stress: an evolving definition. Fac Rev. 2021;10:13.
  • Podyacheva EY, Kushnareva EA, Karpov AA, Toropova YG. Analysis of Models of Doxorubicin-induced cardiomyopathy in rats and mice. A modern view from the perspective of the pathophysiologist and the clinician. Front Pharmacol. 2021;12:670479.
  • Greathouse KL, Samuels M, DiMarco NM, Criswell DS. Effects of increased dietary fat and exercise on skeletal muscle lipid peroxidation and antioxidant capacity in male rats. Eur J Nutr. 2005;44(7):429-435.
  • Zhaobao W, Bing Y. Gastrodia elata Blume extract ameliorates exercise induced fatigue. Afr. J. Biotechnol. 2010;9:5978-5982.
  • Karakoç A. Paraoksonaz polimorfizmleri ile tip 2 diabetin makro ve mikrovasküler komplikasyonları arasındaki ilişkinin araştırılması. Sağlık Bilimleri Enstitüsü, Biyokimya Anabilimdalı, Yüksek Lisans Tezi, Erzurum: Atatürk Üniversitesi, 2008.
  • Tietze F. Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues. Anal. Biochem. 1969;27:502-522.
  • Fairbans VF, Klee GG. Biochemical aspects of hematology. In: CoA. Burtis and ER Ashwood, Editors, Tietz Textbook of clinical Chemistry, Sounders, Philadelphia. 1999: 1991-2106.
  • Alp HH. Hiper ve Hipotiroidili Hastalarda Homosistein S-Adenozilmetiyonin ve Antioksidan Düzeyleri. Sağlık Bilimleri Enstitüsü, Biyokimya Anabilim Dalı. Yüksek Lisans Tezi, Erzurum: Atatürk Üniversitesi, 2005.
  • Paglia DE, Valentina WN. Studies on the quantitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 1967;70:158-169.
  • Yavaş ID, Zengin M, Kaklıkaya İ, Uzun Z, Değer O, Özcan F ve ark. Serbest oksijen radikal temizleyici olarak aprotinin’in rolü, Türk Göğüs Kalp Damar Cer. Derg. 1994;2:208-215.
  • Mc Queen MJ. Optimal Assay of LDH and α-HBD at 37 °C. Ann. Clin. Biochem. 1972;9(1-6):21-25.
  • Yılmaz İ. Antioksidan içeren bazı gıdalar ve oksidatif stres. İnönü Üniversitesi Tıp Fakültesi Dergisi. 2010;17:143-153.
  • Halliwell B, Chirico S. Lipid peroxidation: Its mechanism, measurement and significance. Am. J. Clin. Nutr. 1993; 57: 715-725.
  • Temiz A, Baskurt OK, Pekçetin C, Kandemir F, Güre A. Leukocyte activation, oxidant stress and red blood cell properties after acute, exhausting exercise in rats. Clin. Hemorheol. Microcirc. 2000;22:253–259.
  • Semin I, Kayatekin BM, Gönenç S, Açıkgöz E, Uysal N, Delen Y et al. Lipid peroxidation and antioxidant enzyme levels of intestinal and muscle tissues aftere a 60 minutes exercise in tarined mice, Indian J. Physiol. Pharmacol. 2000,44:419–427.
  • Hara M, Ligo M, Othani-Kaneko R, Nakamura N, Suzuki T, Reiter RJ et al. Administration of melatonin and related indoles prevents exercise-induced cellular oxidative changes in rats. Biol. Signals Recept. 1997;6:90-100.
  • Khanna S, Atalay M, Laaksonen DE, Gül M, Roy S, Sen CK. Alpha-lipoic acid supplementation: tissue glutathione homeostasis at rest and after exercise. J. Appl. Physiol. 1999;86:1191-1196.
  • Al-Majed AA, Gado AM, Al-Shabanah OA, Mansour MA. Alpha-Lipoic acid ameliorates myocardial toxicity induced by doxorubicin. Pharmacol. Res. 2002;46:499-503.
  • Lee SR, Im KJ, Suh SI, Jung JG. Protective effect of green tea polyphenol (-) epigallocatechin gallate and other antioxidants on lipid peroxidation in gerbil brain homogenates. Phytother. Res. 2003;17:206-209.
  • Gök V, Kayacıer A, Telli R. Hayvansal ve mikrobiyal kaynaklı antioksidanlar. Gıda Tek. Elektronik Derg. 2006;2:35-40.
  • Güleşci N, Aygül İ. Beslenmede yer alan antioksidan ve fenolik madde içerikli çerezler. GUJHS. 2016;5(1):109-129.
  • Kim KH, Lee B, Kim YR, Kim MA, Ryu N, Jung DJ, Kim UK et al. Evaluating protective and therapeutic effects of alpha-lipoic acid on cisplatin-induced ototoxicity. Cell Death Dis. 2018;1;9(8):827.
  • Prahalathan C, Selvakumar E, Varalakshmi P. Lipoic acid ameliorates adriamycin induced testicular mitochondriopathy. Reprod Toxicol. 2005;20:111-116.
  • Selvakumar E, Prahalathan C, Sudharsan PT, Varalakshmi P. Chemoprotective effect of lipoic acid against cyclophosphamide-induced changes in the rat sperm. J. Toxicol. 2006;217:71-78.

Investigations of The Alpha-Lipoic Acid Administration on Lipid Peroxidation, Creatin Kinase and Lactate Levels of Mice Exposed to Swimming Exercise

Year 2023, Volume: 18 Issue: 1, 102 - 107, 16.03.2023
https://doi.org/10.17517/ksutfd.1080134

Abstract

Objective: In this study, the effect of alpha-lipoic acid (ALA) administration on the lipid peroxidation, kreatin kinase and lactate levels in mice exposed to swimming exercise was examined.
Materials and Methods: In this study 30 adult Swiss albino mice weighing 20-25 g were used. The mice were divided into three groups as control, ALA-50 and ALA-100 each consisting of 10. The mice in control group received intraperitoneal (i.p) isotonic serum throughout 10 days. The ALA-50 and ALA-100 groups were given 50 and 100 mg/kg concentrated ALA, i.p. At the end of the 10th day, the mice were taken into the swimming pools and subjected to swimming test. After the swimming test, the muscles and blood samples of the mice were collected and analyzed biochemically.
Results: When the data obtained were compared with the control group, there was an increase at the swimming period, GSH, GSH-Px and SOD values, and a decrease at the levels of CK, LDH and MDA.
Conclusion: As a result, the presence of the damage in muscle tissues of the mice performed acute swimming exercise was biochemically detected. We believe that it will be possible to be able to preventing or delaying oxidative damage and muscle fatigue caused by strenuous swimming exercise by the administration of alpha lipoic acid.

Project Number

Yok

References

  • Kin A. Step ve Aerobik Dansın Üniversiteli Bayanların Fizyolojik Parametrelerine Etkisinin Karşılaştırılması, Yüksek Lisans Tezi; Ankara: ODTÜ, 1996.
  • Baltacı Kasım A. Çocuklarda yüzme egzersizinin solunum parametrelerine etkisi. Sağlık Bilimleri Enstitüsü, Fizyoloji Anabilim Dalı, Yüksek Lisans Tezi:, Konya: Selçuk Üniverisitesi, 1990.
  • Stanford D, Stanforth PR, Velasquez KS. Aerobic Requirement of bench stepping. Int. J. Sports Med. 1993;14:129-133.
  • Turanlıv AY. Deri Yaşlanmasında Serbest Radikallerin Yeri, 3. Uludağ Dermatokozmetotloji Günleri Sempozyumu, 2005:100-106.
  • Hajam YA, Rani R, Ganie SY, Sheikh TA, Javaid D, Qadri SS et al. Oxidative stress in human pathology and aging: molecular mechanisms and perspectives. Cells. 2022;11(3):552.
  • Barghi N, Bambaeichi E, Rezaei-Tavirani M, Khaledi N. Aerobic exercises ınduce antioxidant pathways activation in rats. Int J Prev Med. 2020;11:144.
  • Öztürkcan S, Kayhan TÇ. Deri yaşlanmasına karşı medikal önlemler, Dermatoz 2010;1:77-82.
  • Lu Y, Wiltshire HD, Baker JS, Wang Q. Effects of high ıntensity exercise on oxidative stress and antioxidant status in untrained humans: A Systematic Review. Biology (Basel). 2021;10(12):1272.
  • Pala R, Beyaz F, Tuzcu M, Er B, Sahin N, Cinar V ve ark. The effects of coenzyme Q10 on oxidative stress and heat shock proteins in rats subjected to acute and chronic exercise. J. Exerc. Nutrition Biochem. 2018;22(3):14-20.
  • Liang JF, Akaike T. Inhibition of nitric oxide synthesis in primary cultured Mouse hepatocytes by α-lipoic acid. Chem. Biol. Interact. 2000, 124:53-60.
  • St Clair D, Zhao Y, Chaiswing L, Oberley T. Modulation of skin tumorigenesis by SOD. Biomed. Pharmacother. 2005;59:209-214.
  • Salehi B, Berkay Yılmaz Y, Antika G, Boyunegmez Tumer T et al. Insights on the Use of α-Lipoic Acid for Therapeutic Purposes. Biomolecules. 2019;9(8):356.
  • Çakatay U. Pro-oxidant actions of α-lipoic acid and dihydrolipoic acid. Med. Hypotheses. 2006,66:110-117.
  • Ji LL, Yeo D. Oxidative stress: an evolving definition. Fac Rev. 2021;10:13.
  • Podyacheva EY, Kushnareva EA, Karpov AA, Toropova YG. Analysis of Models of Doxorubicin-induced cardiomyopathy in rats and mice. A modern view from the perspective of the pathophysiologist and the clinician. Front Pharmacol. 2021;12:670479.
  • Greathouse KL, Samuels M, DiMarco NM, Criswell DS. Effects of increased dietary fat and exercise on skeletal muscle lipid peroxidation and antioxidant capacity in male rats. Eur J Nutr. 2005;44(7):429-435.
  • Zhaobao W, Bing Y. Gastrodia elata Blume extract ameliorates exercise induced fatigue. Afr. J. Biotechnol. 2010;9:5978-5982.
  • Karakoç A. Paraoksonaz polimorfizmleri ile tip 2 diabetin makro ve mikrovasküler komplikasyonları arasındaki ilişkinin araştırılması. Sağlık Bilimleri Enstitüsü, Biyokimya Anabilimdalı, Yüksek Lisans Tezi, Erzurum: Atatürk Üniversitesi, 2008.
  • Tietze F. Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues. Anal. Biochem. 1969;27:502-522.
  • Fairbans VF, Klee GG. Biochemical aspects of hematology. In: CoA. Burtis and ER Ashwood, Editors, Tietz Textbook of clinical Chemistry, Sounders, Philadelphia. 1999: 1991-2106.
  • Alp HH. Hiper ve Hipotiroidili Hastalarda Homosistein S-Adenozilmetiyonin ve Antioksidan Düzeyleri. Sağlık Bilimleri Enstitüsü, Biyokimya Anabilim Dalı. Yüksek Lisans Tezi, Erzurum: Atatürk Üniversitesi, 2005.
  • Paglia DE, Valentina WN. Studies on the quantitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 1967;70:158-169.
  • Yavaş ID, Zengin M, Kaklıkaya İ, Uzun Z, Değer O, Özcan F ve ark. Serbest oksijen radikal temizleyici olarak aprotinin’in rolü, Türk Göğüs Kalp Damar Cer. Derg. 1994;2:208-215.
  • Mc Queen MJ. Optimal Assay of LDH and α-HBD at 37 °C. Ann. Clin. Biochem. 1972;9(1-6):21-25.
  • Yılmaz İ. Antioksidan içeren bazı gıdalar ve oksidatif stres. İnönü Üniversitesi Tıp Fakültesi Dergisi. 2010;17:143-153.
  • Halliwell B, Chirico S. Lipid peroxidation: Its mechanism, measurement and significance. Am. J. Clin. Nutr. 1993; 57: 715-725.
  • Temiz A, Baskurt OK, Pekçetin C, Kandemir F, Güre A. Leukocyte activation, oxidant stress and red blood cell properties after acute, exhausting exercise in rats. Clin. Hemorheol. Microcirc. 2000;22:253–259.
  • Semin I, Kayatekin BM, Gönenç S, Açıkgöz E, Uysal N, Delen Y et al. Lipid peroxidation and antioxidant enzyme levels of intestinal and muscle tissues aftere a 60 minutes exercise in tarined mice, Indian J. Physiol. Pharmacol. 2000,44:419–427.
  • Hara M, Ligo M, Othani-Kaneko R, Nakamura N, Suzuki T, Reiter RJ et al. Administration of melatonin and related indoles prevents exercise-induced cellular oxidative changes in rats. Biol. Signals Recept. 1997;6:90-100.
  • Khanna S, Atalay M, Laaksonen DE, Gül M, Roy S, Sen CK. Alpha-lipoic acid supplementation: tissue glutathione homeostasis at rest and after exercise. J. Appl. Physiol. 1999;86:1191-1196.
  • Al-Majed AA, Gado AM, Al-Shabanah OA, Mansour MA. Alpha-Lipoic acid ameliorates myocardial toxicity induced by doxorubicin. Pharmacol. Res. 2002;46:499-503.
  • Lee SR, Im KJ, Suh SI, Jung JG. Protective effect of green tea polyphenol (-) epigallocatechin gallate and other antioxidants on lipid peroxidation in gerbil brain homogenates. Phytother. Res. 2003;17:206-209.
  • Gök V, Kayacıer A, Telli R. Hayvansal ve mikrobiyal kaynaklı antioksidanlar. Gıda Tek. Elektronik Derg. 2006;2:35-40.
  • Güleşci N, Aygül İ. Beslenmede yer alan antioksidan ve fenolik madde içerikli çerezler. GUJHS. 2016;5(1):109-129.
  • Kim KH, Lee B, Kim YR, Kim MA, Ryu N, Jung DJ, Kim UK et al. Evaluating protective and therapeutic effects of alpha-lipoic acid on cisplatin-induced ototoxicity. Cell Death Dis. 2018;1;9(8):827.
  • Prahalathan C, Selvakumar E, Varalakshmi P. Lipoic acid ameliorates adriamycin induced testicular mitochondriopathy. Reprod Toxicol. 2005;20:111-116.
  • Selvakumar E, Prahalathan C, Sudharsan PT, Varalakshmi P. Chemoprotective effect of lipoic acid against cyclophosphamide-induced changes in the rat sperm. J. Toxicol. 2006;217:71-78.
There are 37 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Araştırma Makaleleri
Authors

Mürşit Ceyhun Birinci 0000-0002-7258-1217

Ali Karadeniz 0000-0002-0976-9380

Project Number Yok
Publication Date March 16, 2023
Submission Date February 28, 2022
Acceptance Date March 19, 2022
Published in Issue Year 2023 Volume: 18 Issue: 1

Cite

AMA Birinci MC, Karadeniz A. Yüzme Egzersizi Yaptırılan Farelere Alfa-Lipoik Asit Uygulamasının Lipid Peroksidasyon, Kreatin Kinaz ve Laktat Düzeyleri Üzerine Etkisinin İncelenmesi. KSU Medical Journal. March 2023;18(1):102-107. doi:10.17517/ksutfd.1080134