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Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi

Year 2017, Volume: 20 Issue: 4, 361 - 367, 10.04.2017
https://doi.org/10.18016/ksudobil.288175

Abstract

Primula vulgaris, Primula cinsine mensup bir bitki olup, bu bitkiler sıklıkla
geleneksel tedavide kullanılmaktadırlar. Farklı Primula türlerinin farklı ekstraktlarının antioksidan ve sitotoksik
etkilerini konu alan çok sayıda çalışma olmasına rağmen, P. vulgaris ekstraktlarının bu özellikleri ile ilgili sınırlı
sayıda çalışma bulunmaktadır. Bu çalışmanın amacı P. vulgaris yapraklarının dimetil sülfoksitli ekstraktının
antioksidan özelliklerinin ve sitotoksik etkilerinin belirlenmesidir.
Ekstraktın toplam fenolik madde miktarı, toplam flavonoid madde miktarı ve
indirgeyici antioksidan güç tayini spektrofotometrik yöntemler kullanılarak
belirlendi. Ekstraktın sitotoksik etkisi ise insan akciğer (A549), karaciğer
(HepG2), meme (MCF-7), prostat (PC-3) ve kolon (WiDr) kanser hücre serileri ile
normal insan fibroblast hücre serisi üzerinde MTT yöntemi kullanılarak
değerlendirildi. Ekstraktın toplam fenolik madde içeriği ve indirgeyici
antioksidan güç değerleri 100 g örnek başına sırasıyla 1890.1±11.2 mg gallik
asit eşdeğeri ve 3233.1±176.2 mg troloks eşdeğeri olarak bulundu. Ekstrakt
çalışılan tüm kanser hücre serileri üzerinde normal fibroblast hücrelerine göre
seçici sitotoksik etki gösterdi ve IC50 değerleri 133.3-253.8 µg/mL
arasında hesaplandı. Bu çalışma P.
vulgaris
yaprak ekstraktının antioksidan özelliklerini ve kanser hücre
serileri üzerindeki sitotoksik etkisini ortaya koyan ilk çalışmadır. Ekstraktta
bulunan antioksidan ve sitotoksik etki gösteren molekülleri ve bu etkilerin
mekanizmalarını belirleyebilmek için daha ileri çalışmalar gerekmektedir.

References

  • Aliyazicioglu Y, Demir S, Turan I, Cakiroglu TN, Akalin I, Deger O, Bedir A 2011. Preventive and protective effects of Turkish propolis on H2O2 induced DNA damage in foreskin fibroblast cell lines. Acta Biologica Hungarica, 62(4): 388-396.
  • Amirghofran Z, Bahmani M, Azadmehr A, Ashouri E, Javidnia K 2007. Antitumor activity and apoptosis induction in human cancer cell lines by Dionysia termeana. Cancer Investigation, 25: 550-554.
  • Aslam K, Nawchoo IA, Bhat MA, Ganie AH, Aslam N 2014. Ethno-pharmacological review of genus Primula. International Journal of Advanced Research, 2(4): 29-34.
  • Aslam K, Nawchoo IA, Ganai BA 2015. In vitro antioxidant, antibacterial activity and phytochemical studies of Primula Denticulata-an important medicinal pant of Kashmir Himalaya. International Journal of Pharmacological Research, 5(3): 49-56.
  • Behzad S, Pirani A, Mosaddegh M 2014. Cytotoxic activity of some medicinal plants from Hamedan district of Iran. Iranian Journal of Pharmaceutical Research, 13: 199-205.
  • Behzad S, Ebrahim K, Mosaddegh M, Haerid A 2016. Primula auriculata extracts exert cytotoxic and apoptotic effects against HT-29 human colon adenocarcinoma cells. Iranian Journal of Pharmaceutical Research, 15(1): 311-322.
  • Bufalo MC, Candeias JM, Sforcin JM 2009. In vitro cytotoxic effect of Brazilian green propolis on human laryngeal epidermoid carcinoma (HEp-2) cells. Evidince-Based Complementary and Alternative Medicine, 6(4): 483-487.
  • Dai J, Mumper RJ 2010. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15: 7313-7352.
  • Demir N, Alayli Gungor A, Nadaroglu H, Demir Y 2014. The antioxidant and radical scavenging activities of Primrose (Primula vulgaris). European Journal of Experimental Biology, 4(2): 395-401.
  • Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Ozer Yaman S, Akbulut K, Kilinc K, Deger O 2016a. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutrition and Cancer, 68(1): 165-172.
  • Demir S, Turan I, Aliyazicioglu Y 2016b. Selective cytotoxic effect of Rhododendron luteum extract on human colon and liver cancer cells. Journal of Balkan Union of Oncology, 21(4): 883-888.
  • Hu ML 2011. Dietary polyphenols as antioxidants and anticancer agents: more questions than answers. Chang Gung Medical Journal, 34(5): 449-460.
  • Hussain SA, Sulaiman AA, Balch C, Chauhan H, Alhadidi QM, Tiwari AK 2016. Natural polyphenols in cancer chemoresistance. Nutrition and Cancer, 68(6): 879-891.
  • Jaberian H, Piri K, Nazari J 2013. Phytochemical composition and in vitro antimicrobial and antioxidant activities of some medicinal plants. Food Chemistry, 136: 237-244.
  • Kaur C, Kapoor HC 2001. Antioxidants in fruits and vegetables-the millennium's health. International Journal of Food Science and Technology, 36: 703-725.
  • Moreno MI, Isla MI, Sampietro AR, Vattuone MA 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71(1-2): 109-114.
  • Mosmann T 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65: 55-63.
  • Mostafa FA, Gamal MA, Sabrin IRM, Ehab, ES 2014. Antioxidant and anti-inflamatory activities of phenolic constituents from Primula elatior L. aerial part. International Journal of Pharmacognosy and Phytochemical Research, 6(1): 74-78.
  • Najmus-Saqib Q, Alam F, Ahmad M 2009. Antimicrobial and cytotoxicity activities of the medicinal plant Primula macrophylla. Journal of Enzyme Inhibition and Medicinal Chemistry, 24(3): 697-701.
  • Orhan DD, Ozcelik B, Hosbas S, Vural M 2012. Assessment of antioxidant, antibacterial, antimycobacterial, and antifungal activities of some plants used as folk remedies in Turkey against dermatophytes and yeast-like fungi. Turkish Journal of Biology, 36: 672-686.
  • Oyaizu M 1986. Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition, 44: 307-315.
  • Pehlivan Karakas F, Birinci Yildirim A, Bayram R, Yavuz MZ, Gepdiremen A, Ucar Turker A 2015. Antiproliferative activity of some medicinal plants on human breast and hepatocellular carcinoma cell lines and their phenolic contents. Tropical Journal of Pharmaceutical Research, 14(10): 1787-1795.
  • Ravishankar D, Rajora AK, Greco F, Osborn HM 2013. Flavonoids as prospective compounds for anti-cancer therapy. International Journal of Biochemistry & Cell Biology, 45(12): 2821-2831.
  • Shostak LG, Marchyshyn SM, Kozachok SS, Karbovska RV 2016. Investigation of phenolic compounds of Primula veris L. Journal of Education, Health and Sport, 6(5): 424-432.
  • Singh S, Ali S, Singh M 2014. Biological screening of plants extract showing hypoglycaemic and wound healing properties: Capparis zeylanica and Primula denticulata. American Journal of Phytomedicine and Clinical Therapeutics, 2(12): 1338-1345.
  • Slinkard K, Singleton VL 1977. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28: 49-55.
  • Surh YJ 2003. Cancer chemoprevention with dietary phytochemicals. Nature Reviews Cancer, 3(10): 768-780.
  • Tokalov SV, Kind B, Wollenweber E, Gutzeit HO 2004. Biological effects of epicuticular flavonoids from Primula denticulata on human leukemia cells. Journal of Agricultural and Food Chemistry, 52(2): 239-245.
  • Turan I, Deger O, Aliyazicioglu Y, Demir S, Kilinc K, Sumer A 2015. Effects of Turkish propolis on expression of hOGG-1 and NEIL-1. Turkish Journal of Medical Sciences, 45: 804-811.
  • Turker AU, Usta C 2008. Biological screening of some Turkish medicinal plant extracts for antimicrobial and toxicity activities. Natural Product Research, 22(2): 136-146.
  • Unnati S, Ripal S, Sanjeev A, Niyati A 2013. Novel anticancer agents from plant sources. Chinese Journal of Natural Medicines, 11(1): 16-23.
  • Weyermann J, Lochmann D, Zimmer A 2005. A practical note on the use of cytotoxicity assays. International Journal of Pharmaceutics, 288: 369-376.

Evaluation of Antioxidant and Cytotoxic Properties of Primula vulgaris Leaf Extract

Year 2017, Volume: 20 Issue: 4, 361 - 367, 10.04.2017
https://doi.org/10.18016/ksudobil.288175

Abstract

Primula vulgaris belongs to the genus Primula and this genus is frequently used in traditional medicine.
Numerous studies reported the antioxidant and cytotoxic effects of various
extracts of different Primula species
but, there were limited studies of the antioxidant and cytotoxic effect of P. vulgaris. The aim of this study was
to determine the antioxidant properties and cytotoxic effects of dimethyl
sulfoxide extract of leaves of P.
vulgaris
. The total polyphenolic content (TPC), total flavonoid content
(TFC) and reducing antioxidant power of extract were evaluated using
spectrophotometric procedures. The cytotoxic effects of extract were revealed
on human lung (A549), liver (HepG2), breast (MCF-7), prostate (PC-3), and colon
(WiDr) cancer cell lines and normal fibroblast cells using thiazolyl blue tetrazolium
bromide (MTT) assay. TPC and FRAP values were 1890.1±11.2 mg gallic acid
equivalents and 3233.1±176.2 mg trolox equivalents per 100 g sample,
respectively. The extract showed selective cytotoxic effects on all studied
cancer cell lines compared to normal fibroblast cells and IC50
values ranged from 133.3-253.8 µg/mL. This is the first study to reveal the
antioxidant properties of P. vulgaris
leaf extract and its cytotoxic effect on cancer cell lines. Further studies are
now needed to identify the antioxidant and cytotoxic molecules in the extract
and their mechanisms.

References

  • Aliyazicioglu Y, Demir S, Turan I, Cakiroglu TN, Akalin I, Deger O, Bedir A 2011. Preventive and protective effects of Turkish propolis on H2O2 induced DNA damage in foreskin fibroblast cell lines. Acta Biologica Hungarica, 62(4): 388-396.
  • Amirghofran Z, Bahmani M, Azadmehr A, Ashouri E, Javidnia K 2007. Antitumor activity and apoptosis induction in human cancer cell lines by Dionysia termeana. Cancer Investigation, 25: 550-554.
  • Aslam K, Nawchoo IA, Bhat MA, Ganie AH, Aslam N 2014. Ethno-pharmacological review of genus Primula. International Journal of Advanced Research, 2(4): 29-34.
  • Aslam K, Nawchoo IA, Ganai BA 2015. In vitro antioxidant, antibacterial activity and phytochemical studies of Primula Denticulata-an important medicinal pant of Kashmir Himalaya. International Journal of Pharmacological Research, 5(3): 49-56.
  • Behzad S, Pirani A, Mosaddegh M 2014. Cytotoxic activity of some medicinal plants from Hamedan district of Iran. Iranian Journal of Pharmaceutical Research, 13: 199-205.
  • Behzad S, Ebrahim K, Mosaddegh M, Haerid A 2016. Primula auriculata extracts exert cytotoxic and apoptotic effects against HT-29 human colon adenocarcinoma cells. Iranian Journal of Pharmaceutical Research, 15(1): 311-322.
  • Bufalo MC, Candeias JM, Sforcin JM 2009. In vitro cytotoxic effect of Brazilian green propolis on human laryngeal epidermoid carcinoma (HEp-2) cells. Evidince-Based Complementary and Alternative Medicine, 6(4): 483-487.
  • Dai J, Mumper RJ 2010. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15: 7313-7352.
  • Demir N, Alayli Gungor A, Nadaroglu H, Demir Y 2014. The antioxidant and radical scavenging activities of Primrose (Primula vulgaris). European Journal of Experimental Biology, 4(2): 395-401.
  • Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Ozer Yaman S, Akbulut K, Kilinc K, Deger O 2016a. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutrition and Cancer, 68(1): 165-172.
  • Demir S, Turan I, Aliyazicioglu Y 2016b. Selective cytotoxic effect of Rhododendron luteum extract on human colon and liver cancer cells. Journal of Balkan Union of Oncology, 21(4): 883-888.
  • Hu ML 2011. Dietary polyphenols as antioxidants and anticancer agents: more questions than answers. Chang Gung Medical Journal, 34(5): 449-460.
  • Hussain SA, Sulaiman AA, Balch C, Chauhan H, Alhadidi QM, Tiwari AK 2016. Natural polyphenols in cancer chemoresistance. Nutrition and Cancer, 68(6): 879-891.
  • Jaberian H, Piri K, Nazari J 2013. Phytochemical composition and in vitro antimicrobial and antioxidant activities of some medicinal plants. Food Chemistry, 136: 237-244.
  • Kaur C, Kapoor HC 2001. Antioxidants in fruits and vegetables-the millennium's health. International Journal of Food Science and Technology, 36: 703-725.
  • Moreno MI, Isla MI, Sampietro AR, Vattuone MA 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71(1-2): 109-114.
  • Mosmann T 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65: 55-63.
  • Mostafa FA, Gamal MA, Sabrin IRM, Ehab, ES 2014. Antioxidant and anti-inflamatory activities of phenolic constituents from Primula elatior L. aerial part. International Journal of Pharmacognosy and Phytochemical Research, 6(1): 74-78.
  • Najmus-Saqib Q, Alam F, Ahmad M 2009. Antimicrobial and cytotoxicity activities of the medicinal plant Primula macrophylla. Journal of Enzyme Inhibition and Medicinal Chemistry, 24(3): 697-701.
  • Orhan DD, Ozcelik B, Hosbas S, Vural M 2012. Assessment of antioxidant, antibacterial, antimycobacterial, and antifungal activities of some plants used as folk remedies in Turkey against dermatophytes and yeast-like fungi. Turkish Journal of Biology, 36: 672-686.
  • Oyaizu M 1986. Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition, 44: 307-315.
  • Pehlivan Karakas F, Birinci Yildirim A, Bayram R, Yavuz MZ, Gepdiremen A, Ucar Turker A 2015. Antiproliferative activity of some medicinal plants on human breast and hepatocellular carcinoma cell lines and their phenolic contents. Tropical Journal of Pharmaceutical Research, 14(10): 1787-1795.
  • Ravishankar D, Rajora AK, Greco F, Osborn HM 2013. Flavonoids as prospective compounds for anti-cancer therapy. International Journal of Biochemistry & Cell Biology, 45(12): 2821-2831.
  • Shostak LG, Marchyshyn SM, Kozachok SS, Karbovska RV 2016. Investigation of phenolic compounds of Primula veris L. Journal of Education, Health and Sport, 6(5): 424-432.
  • Singh S, Ali S, Singh M 2014. Biological screening of plants extract showing hypoglycaemic and wound healing properties: Capparis zeylanica and Primula denticulata. American Journal of Phytomedicine and Clinical Therapeutics, 2(12): 1338-1345.
  • Slinkard K, Singleton VL 1977. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28: 49-55.
  • Surh YJ 2003. Cancer chemoprevention with dietary phytochemicals. Nature Reviews Cancer, 3(10): 768-780.
  • Tokalov SV, Kind B, Wollenweber E, Gutzeit HO 2004. Biological effects of epicuticular flavonoids from Primula denticulata on human leukemia cells. Journal of Agricultural and Food Chemistry, 52(2): 239-245.
  • Turan I, Deger O, Aliyazicioglu Y, Demir S, Kilinc K, Sumer A 2015. Effects of Turkish propolis on expression of hOGG-1 and NEIL-1. Turkish Journal of Medical Sciences, 45: 804-811.
  • Turker AU, Usta C 2008. Biological screening of some Turkish medicinal plant extracts for antimicrobial and toxicity activities. Natural Product Research, 22(2): 136-146.
  • Unnati S, Ripal S, Sanjeev A, Niyati A 2013. Novel anticancer agents from plant sources. Chinese Journal of Natural Medicines, 11(1): 16-23.
  • Weyermann J, Lochmann D, Zimmer A 2005. A practical note on the use of cytotoxicity assays. International Journal of Pharmaceutics, 288: 369-376.
There are 32 citations in total.

Details

Journal Section MİKROBİYOLOJİ (Microbiology)
Authors

Selim Demir

İbrahim Turan

Rezzan Aliyazıcıoğlu

Yüksel Aliyazıcıoğlu

Publication Date April 10, 2017
Acceptance Date February 21, 2017
Published in Issue Year 2017 Volume: 20 Issue: 4

Cite

APA Demir, S., Turan, İ., Aliyazıcıoğlu, R., Aliyazıcıoğlu, Y. (2017). Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi. KSÜ Doğa Bilimleri Dergisi, 20(4), 361-367. https://doi.org/10.18016/ksudobil.288175
AMA Demir S, Turan İ, Aliyazıcıoğlu R, Aliyazıcıoğlu Y. Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi. KSÜ Doğa Bilimleri Dergisi. June 2017;20(4):361-367. doi:10.18016/ksudobil.288175
Chicago Demir, Selim, İbrahim Turan, Rezzan Aliyazıcıoğlu, and Yüksel Aliyazıcıoğlu. “Primula Vulgaris Yaprak Ekstraktının Antioksidan Ve Sitotoksik Özelliklerinin Değerlendirilmesi”. KSÜ Doğa Bilimleri Dergisi 20, no. 4 (June 2017): 361-67. https://doi.org/10.18016/ksudobil.288175.
EndNote Demir S, Turan İ, Aliyazıcıoğlu R, Aliyazıcıoğlu Y (June 1, 2017) Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi. KSÜ Doğa Bilimleri Dergisi 20 4 361–367.
IEEE S. Demir, İ. Turan, R. Aliyazıcıoğlu, and Y. Aliyazıcıoğlu, “Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi”, KSÜ Doğa Bilimleri Dergisi, vol. 20, no. 4, pp. 361–367, 2017, doi: 10.18016/ksudobil.288175.
ISNAD Demir, Selim et al. “Primula Vulgaris Yaprak Ekstraktının Antioksidan Ve Sitotoksik Özelliklerinin Değerlendirilmesi”. KSÜ Doğa Bilimleri Dergisi 20/4 (June 2017), 361-367. https://doi.org/10.18016/ksudobil.288175.
JAMA Demir S, Turan İ, Aliyazıcıoğlu R, Aliyazıcıoğlu Y. Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi. KSÜ Doğa Bilimleri Dergisi. 2017;20:361–367.
MLA Demir, Selim et al. “Primula Vulgaris Yaprak Ekstraktının Antioksidan Ve Sitotoksik Özelliklerinin Değerlendirilmesi”. KSÜ Doğa Bilimleri Dergisi, vol. 20, no. 4, 2017, pp. 361-7, doi:10.18016/ksudobil.288175.
Vancouver Demir S, Turan İ, Aliyazıcıoğlu R, Aliyazıcıoğlu Y. Primula vulgaris Yaprak Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin Değerlendirilmesi. KSÜ Doğa Bilimleri Dergisi. 2017;20(4):361-7.