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Isolation of fungi associated with Meloidogyne incognita and their effect on its eggs hatching in the Green Mountain region of Libya

Year 2017, Volume: 17 Issue: 2, 362 - 367, 28.09.2017
https://doi.org/10.17475/kastorman.288092

Abstract

Abstract

Aim of study:
The aim of the study was to isolate the fungi associated with root-knot
nematodes and to determine their effect on its eggs hatching in the Green
Mountain region of Libya.

Material and methods: Soil samples infected with root-knot nematode were collected from the
some farms of the Green Mountain region in Libya. Sample collection was carried
out during May 2007 within ten different areas. Two fields planted with tomato
plants were chosen from the each area. Different types of culture media were
used to isolate the fungus species. Fifteen fungi were isolated from the
rhizosphere of infected plants. Then, the effect of some isolated and
identified fungi on the rate of hatching of nematode eggs was tested.

Main results:
Isolation results indicate that some of the isolated fungi were considered as
parasites on the nematodes. In the study area, Arthrobotrys sp. had the highest percentage of occurrence (70%) and
the least one was Penicillium sp.
(12.25%). Three inoculum levels of 0.75, 1.5, 2×106 fungal propagules were
tested and the highest hatching inhibition (90.5%) was recorded in Paecilomyces lilacinus and the lowest
inhibition level (58.4%) was observed in Vertecillium
chlamydosporium
.









Research highlights: As a result of this study, more successful outcomes can be obtained
during the biological control of root-knot nematodes.   

References

  • Agrios, G.N., 1985. Plant pathology. University of Massachusetts. Boston. United States of America. Academic Press Inc.III Fifth Avenue New York
  • Ahmed, M.A., 1998. The world of fungi. Arab for Publication, Egypt.
  • Al-Assas, K.M., 2003. Introduction to plant nematodes. Damascus University. Syria.
  • Al-Bony, M.A., 1990. Basics practical fungi. Tripoli University. Libya.
  • Alhazmi, A.S., 1992. Introduction to Nematology plant. Faculty of Agriculture. King Saud University. Kingdom of Saudi Arabia.
  • Barnett, H.L. and Hunter, B.B., 1972. Illustrated genera of imperfect fungi. 3rd Edition, Burgess Publishing Co.
  • Baron, G.L., 1969. Isolation and maintenance of endoparasitic nematophagous hyphmycetes. Canadian Journal of Botany, 47, 1899-1902.
  • Bin-soud, S.R., Al-dongly, Z., 2003. Fungus associated with nematodes in the soil in some areas of Libya. Journal of Arab Plant Protection, 21(2), 141-145.
  • Dabaj, H.K., 2003. Applications of soil solarization in greenhouses in Libya. Abstracts of the Eighth Arab Congress of Plant Protection, 12-16 October/2003. 120p.
  • Gapasin, R.M., 1980. Reaction of golden yellow cassava to Meloidogyne spp. Inoculation. Annals of Tropical Research, 2, 49-53.
  • Main, I.H. and Rodriguez, k., 1982. Soil amendments with oil cakes and chicken litter for control of Meloidogyne arenaria. Nematotropica, 12, 202-206.
  • Main, I.H., Goody, G., Shelby, R.A., Rodriguez-Kabana, R. and morgan-Jones, G., 1982. Chitin amendments for control of Meloidogyne arenaria in infested soil. Nematotropica, 12, 71-77.
  • Moens, M., Roland N. P., James, L. S., 2009. Meloidogyne Species: a Diverse Group of Novel and Important Plant Parasites.” In Root-knot Nematodes, ed. Roland N Perry, Maurice Moens, and James L Starr, 1–17. Wallingford, UK: CABI Publishing.
  • Mosa, I.A., 2008. Biological control of root knot nematode using fungi. Master Thesis. Omar Mukhtar University. Libya. 75 p.
  • Saadabi, A., Al-Amein, E., 2001. Isolation and identification of some internal parasitism fungi, and Huntress of nematode in agricultural soil in Sudan. Journal of Arab Plant Protection, 19, 55-58.
  • Stirling, G.R.., West, L.M. 1991. Fungal parasitism of root-knot nematode eggs from tropical and subtropica l regions of Austrelia.Australian Plant Pathology, 11, 215-221.
  • Theberge, R. L., 1985. Common African Pests and Diseases of cassava, Yam, Sweet Potato and Cocoyam. International Institute of Tropical Agriculture (IITA). Ibadan, Nigeria.
  • Toussoun TA, Nelson, PE .,1976. A Pictorial Guide to the Identification of Fusarium Species. Second Edition. Pennsylvania State University Press, University Park. USA.

Libya’nın Green Mountain bölgesinde kök-ur nematodu (Meloidogyne incognita) ile bağlantılı mantarların izolasyonu ve yumurtaların açılımı üzerine etkisi

Year 2017, Volume: 17 Issue: 2, 362 - 367, 28.09.2017
https://doi.org/10.17475/kastorman.288092

Abstract



Özet








Çalışmanın amacı: Bu çalışmada Libya’nın Green Mountain bölgesinde kök-ur nemetodu ile
bağlantılı mantarların izole edilmesi ve bu mantarların kök-ur nemetodu yumurta
açılımları üzerine etkisinin araştırılması amaçlanmaktadır.

Materyal ve yöntem: Bu çalışmada Libya’nın Green Mountain bölgesindeki bazı tarım
alanlarından kök-ur nematodu enfekteli toprak örnekleri alınmıştır. Araziden
örnek alımları Mayıs 2007’de 10 farklı alanda yapılmıştır. Her bir alandan
domates ekili iki tarla seçilerek örneklenmiştir. Mantar türlerini izole etmek
için farklı besi ortamları kullanılmış olup enfekte olmuş bitki
rizosferlerinden 15 mantar türü izole edilmiştir. Daha sonra izole edilip
teşhis edilen bazı mantar türlerinin nematod yumurtaları açılım oranları
üzerine etkisi test edilmiştir.      

Sonuçlar:
Izolasyon sonuçları izole edilen bazı mantar türlerinin nematodlar üzerinde
parazitik özellikte olduğunu göstermiştir. Çalışma alanında
Arthrobotrys
sp. varlığının en yüksek yüzdeye (%70) sahip olduğu, en az görülen türlerin ise
Penicillium sp. (%12.25) olduğu
tespit edilmiştir. Araştırmada mantar üreme yapılarının 3 inokulum seviyesi
(0.75, 1.5 ve 2×106) test edilmiştir. Sonuç olarak, en yüksek yumurta açılımı
engellenme (%90.5) Paecilomyces
lilacinus’
da ve en düşük (%58.4) ise Vertecillium
chlamydosporium
uygulamasında görüldü.       
  







Araştırma vurguları: Bu çalışmanın sonucu olarak, kök-ur nematodu ile biyolojik mücadele
kapsamında daha başarılı sonuçların alınabileceği düşünülmektedir. 
 

References

  • Agrios, G.N., 1985. Plant pathology. University of Massachusetts. Boston. United States of America. Academic Press Inc.III Fifth Avenue New York
  • Ahmed, M.A., 1998. The world of fungi. Arab for Publication, Egypt.
  • Al-Assas, K.M., 2003. Introduction to plant nematodes. Damascus University. Syria.
  • Al-Bony, M.A., 1990. Basics practical fungi. Tripoli University. Libya.
  • Alhazmi, A.S., 1992. Introduction to Nematology plant. Faculty of Agriculture. King Saud University. Kingdom of Saudi Arabia.
  • Barnett, H.L. and Hunter, B.B., 1972. Illustrated genera of imperfect fungi. 3rd Edition, Burgess Publishing Co.
  • Baron, G.L., 1969. Isolation and maintenance of endoparasitic nematophagous hyphmycetes. Canadian Journal of Botany, 47, 1899-1902.
  • Bin-soud, S.R., Al-dongly, Z., 2003. Fungus associated with nematodes in the soil in some areas of Libya. Journal of Arab Plant Protection, 21(2), 141-145.
  • Dabaj, H.K., 2003. Applications of soil solarization in greenhouses in Libya. Abstracts of the Eighth Arab Congress of Plant Protection, 12-16 October/2003. 120p.
  • Gapasin, R.M., 1980. Reaction of golden yellow cassava to Meloidogyne spp. Inoculation. Annals of Tropical Research, 2, 49-53.
  • Main, I.H. and Rodriguez, k., 1982. Soil amendments with oil cakes and chicken litter for control of Meloidogyne arenaria. Nematotropica, 12, 202-206.
  • Main, I.H., Goody, G., Shelby, R.A., Rodriguez-Kabana, R. and morgan-Jones, G., 1982. Chitin amendments for control of Meloidogyne arenaria in infested soil. Nematotropica, 12, 71-77.
  • Moens, M., Roland N. P., James, L. S., 2009. Meloidogyne Species: a Diverse Group of Novel and Important Plant Parasites.” In Root-knot Nematodes, ed. Roland N Perry, Maurice Moens, and James L Starr, 1–17. Wallingford, UK: CABI Publishing.
  • Mosa, I.A., 2008. Biological control of root knot nematode using fungi. Master Thesis. Omar Mukhtar University. Libya. 75 p.
  • Saadabi, A., Al-Amein, E., 2001. Isolation and identification of some internal parasitism fungi, and Huntress of nematode in agricultural soil in Sudan. Journal of Arab Plant Protection, 19, 55-58.
  • Stirling, G.R.., West, L.M. 1991. Fungal parasitism of root-knot nematode eggs from tropical and subtropica l regions of Austrelia.Australian Plant Pathology, 11, 215-221.
  • Theberge, R. L., 1985. Common African Pests and Diseases of cassava, Yam, Sweet Potato and Cocoyam. International Institute of Tropical Agriculture (IITA). Ibadan, Nigeria.
  • Toussoun TA, Nelson, PE .,1976. A Pictorial Guide to the Identification of Fusarium Species. Second Edition. Pennsylvania State University Press, University Park. USA.
There are 18 citations in total.

Details

Journal Section Articles
Authors

İssa Mosa

Mahmoud Ehwaetı This is me

Erol Akkuzu

Publication Date September 28, 2017
Published in Issue Year 2017 Volume: 17 Issue: 2

Cite

APA Mosa, İ., Ehwaetı, M., & Akkuzu, E. (2017). Isolation of fungi associated with Meloidogyne incognita and their effect on its eggs hatching in the Green Mountain region of Libya. Kastamonu University Journal of Forestry Faculty, 17(2), 362-367. https://doi.org/10.17475/kastorman.288092
AMA Mosa İ, Ehwaetı M, Akkuzu E. Isolation of fungi associated with Meloidogyne incognita and their effect on its eggs hatching in the Green Mountain region of Libya. Kastamonu University Journal of Forestry Faculty. September 2017;17(2):362-367. doi:10.17475/kastorman.288092
Chicago Mosa, İssa, Mahmoud Ehwaetı, and Erol Akkuzu. “Isolation of Fungi Associated With Meloidogyne Incognita and Their Effect on Its Eggs Hatching in the Green Mountain Region of Libya”. Kastamonu University Journal of Forestry Faculty 17, no. 2 (September 2017): 362-67. https://doi.org/10.17475/kastorman.288092.
EndNote Mosa İ, Ehwaetı M, Akkuzu E (September 1, 2017) Isolation of fungi associated with Meloidogyne incognita and their effect on its eggs hatching in the Green Mountain region of Libya. Kastamonu University Journal of Forestry Faculty 17 2 362–367.
IEEE İ. Mosa, M. Ehwaetı, and E. Akkuzu, “Isolation of fungi associated with Meloidogyne incognita and their effect on its eggs hatching in the Green Mountain region of Libya”, Kastamonu University Journal of Forestry Faculty, vol. 17, no. 2, pp. 362–367, 2017, doi: 10.17475/kastorman.288092.
ISNAD Mosa, İssa et al. “Isolation of Fungi Associated With Meloidogyne Incognita and Their Effect on Its Eggs Hatching in the Green Mountain Region of Libya”. Kastamonu University Journal of Forestry Faculty 17/2 (September 2017), 362-367. https://doi.org/10.17475/kastorman.288092.
JAMA Mosa İ, Ehwaetı M, Akkuzu E. Isolation of fungi associated with Meloidogyne incognita and their effect on its eggs hatching in the Green Mountain region of Libya. Kastamonu University Journal of Forestry Faculty. 2017;17:362–367.
MLA Mosa, İssa et al. “Isolation of Fungi Associated With Meloidogyne Incognita and Their Effect on Its Eggs Hatching in the Green Mountain Region of Libya”. Kastamonu University Journal of Forestry Faculty, vol. 17, no. 2, 2017, pp. 362-7, doi:10.17475/kastorman.288092.
Vancouver Mosa İ, Ehwaetı M, Akkuzu E. Isolation of fungi associated with Meloidogyne incognita and their effect on its eggs hatching in the Green Mountain region of Libya. Kastamonu University Journal of Forestry Faculty. 2017;17(2):362-7.

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