Research Article
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A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties

Year 2024, Volume: 21 Issue: 1, 265 - 276, 30.01.2024

Burcu Kahraman Burcu Mine Şenol Enes Dertli Muhammet Arıcı

https://doi.org/10.33462/jotaf.1329533

Abstract

Bacillus species are gram-positive, aerobic, peritrically flagellated and endospore-forming bacteria. They can be found everywhere in the environment, especially in soil (its common habitat), water, dust or in the air. Probiotics, which have beneficial health effects, constitute an important group of Bacillus species. This study aimed to isolate Bacillus from various sources, identify it molecularly and determine its probiotic properties.For this purpose, eight Bacillus subtilis, Bacillus coagulans and Bacillus clausii strains among 58 isolates from fish intestine, soil, ripened cheese and commercial probiotic supplements were identified and their probiotic properties were characterized. Firstly, Bacillus strains were molecularly identified by 16S rRNA PCR analysis. The growth of Bacillus isolates at various temperatures, salt concentrations, and pH levels, as well as tests for esculin hydrolysis, starch hydrolysis, nitrate reduction, and gas generation from glucose, were all investigated to assess the isolates' physiological and biochemical characteristics. In terms of probiotic potential of Bacillus isolates; tolerance of bile salt, cell surface hydrophobicity, auto aggregation, antibiotic susceptibility tests were conducted. In all analyses, strains obtained from food supplements showed high levels of hydrophobicity and auto-aggregation properties, and the highest values following these strains were observed in Bacillus subtilis strains (F1 and S2) isolated from fish intestines and soil, respectively. All strains showed strong growth features in bile salt conditions. It has been determined that antibiotic sensitivity varies depending on the strain. Overall, high sensitivity to tetracycline has been observed. In summary, this study revealed the potential probiotic properties of Bacillus isolates obtained from different sources. The study also compared these probiotic properties with probiotic Bacillus strains isolated from food supplements.

Keywords

Bacillus Molecular identification Biochemical characterization Probiotics.

Supporting Institution

TÜBİTAK

Project Number

5190060

Thanks

This article is a part of the project supported by the Scientific and Technological Research Council of Turkey (TUBITAK-TEYDEB; Project Number: 5190060.) Burcu Kahraman is supported by TUBİTAK 2211/A and The Turkish Council of Higher Education 100/2000 programs. Burcu Mine Şenol is supported by TUBİTAK 2210/A program, TURKEY

References

  • Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3): 403-410.
  • Baker, G. C., Smith, J. J. and Cowan, D. A. (2003). Review and re-analysis of domain-specific 16S primers. Journal of Microbiological Methods, 55(3): 541-555.
  • Banerjee, G., Nandi, A. and Ray, A. K. (2017). Assessment of hemolytic activity, enzyme production and bacteriocin characterization of Bacillus subtilis LR1 isolated from the gastrointestinal tract of fish. Archives of Microbiology, 199: 115–124.
  • Bezkorovainy, A. (2001). Probiotics: determinants of survival and growth in the gut. The American Journal of Clinical Nutrition, 73(2 Suppl): 399-405.
  • Chaiyawan, N., Taveeteptaikul, P., Wannissorn, B., Ruengsomwong, S., Klungsupya, P., Buaban, W. and Itsaranuwat, P. (2010). Characterization and Probiotic Properties of Bacillus Strains Isolated from Broiler. The Thai Journal of Veterinary Medicine, 40(2): 207-214.
  • Conway, P. L., Gorbach, S. L., Goldin, B. R. (1987). Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. Journal of Dairy Science, 70(1): 1-12.
  • Cutting, S. M. (2011). Bacillus probiotics. Food Microbiology, 28(2): 214-220.
  • Dabiré, Y., Somda, N. S., Somda, M. K., Compaoré, C. B., Mogmenga, I., Ezeogu, L. I., ... and Dicko, M. H. (2022). Assessment of probiotic and technological properties of Bacillus spp. isolated from Burkinabe Soumbala. BMC Microbiology, 22(1): 1-13.
  • Endres, J. R., Clewell, A., Jade, K. A., Farber, T., Hauswirth, J., Schauss, A. G. (2009). Safety assessment of a proprietary preparation of a novel Probiotic, Bacillus coagulans, as a food ingredient. Food and Chemical Toxicology, 47(6): 1231-1238.
  • Freitas, D. B., Reis, M. P., Lima-Bittencourt, C. I., Costa, P. S., Assis, P. S., Chartone-Souza, E. and Nascimento, A. M. (2008). Genotypic and phenotypic diversity of Bacillus spp. isolated from steel plant waste. BMC Research Notes, 1(1): 92.
  • Gatson, J. W., Benz, B. F., Chandrasekaran, C., Satomi, M., Venkateswaran, K. and Hart, M. E. (2006). Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis. International Journal of Systematic and Evolutionary Microbiology, 56(7): 1475-1484.
  • Ghosh, K., Sen, S. K. and Ray, A. K. (2002). Characterization of Bacilli isolated from the gut of rohu, Labeo rohita, fingerlings and its significance in digestion. Journal of Applied Aquaculture, 12(3): 33-42.
  • Giri, S. S., Sukumaran, V. and Dangi, N. K. (2012). Probiotics and Antimicrobial Proteins, 4(4): 238–242.
  • Green, D. H., Wakeley, P. R., Page, A., Barnes, A., Baccigalupi, L., Ricca, E. and Cutting, S. M. (1999).
  • Characterization of two Bacillus probiotics. Applied and Environmental Microbiology, 65(9): 4288-4291.
  • Güldoğan, Ö., Aktepe, B. P. and Aysan, Y. (2022) Use of different bacillus species in the biological control of tomato bacterial speck disease. Journal of Tekirdag Agricultural Faculty, 19(4): 829-839.
  • Hoa, N. T., Baccigalupi, L., Huxham, A., Smertenko, A., Van, P. H., Ammendola, S., …and Cutting, S. M. (2000). Characterization of Bacillus Species Used for Oral Bacteriotherapy and Bacterioprophylaxis of Gastrointestinal Disorders. Applied and Environmental Microbiology, 66(12): 5241-5247.
  • Hong, H. A., To, E., Fakhry, S., Baccigalupi, L., Ricca, E. and Cutting, S. M. (2009). Defining the natural habitat of Bacillus spore-formers". Research in Microbiology, 160(6): 375-379.
  • İspirli, H., Demirbaş, F. and Dertli, E. (2015). Characterization of functional properties of Enterococcus faecium strains isolated from human gut. Canadian Journal of Microbiology, 61(11): 861-870.
  • Joint FAO/WHO Working Group (2002) Report on Drafting Guidelines for the Evaluation of Probiotics in Food: Guidelines for the Evaluation of Probiotics in Food, London, Ontorio, Canada 30 April-1 May 2002.
  • Kavitha, M., Raja, M. and Perumal, P. (2018). Evaluation of probiotic potential of Bacillus spp. isolated from the digestive tract of freshwater fish Labeo calbasu. Aquaculture Reports, 11: 59-69.
  • Kos, B., Šušković, J., Vuković, S., Šimpraga, M., Frece, J. and Matošić, S. (2003). Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. Journal of Applied Microbiology, 94(6): 981-987.
  • Lei, J., Ran, X., Guo, M., Liu, J., Yang, F. and Chen, D. (2023). Screening, identification, and probiotic properties of Bacillus pumilus from Yak. Probiotics and Antimicrobial Proteins, https://doi.org/10.1007/s12602-023-10054-w
  • Ljungh, A. and Wadström, T. (2006). Lactic acid bacteria as probiotics. Current Issues in Intestinal Microbiology, 7(2): 73-89.
  • Logan, N A., De Vos, P. A. U. L. (2009). Family Bacillaceae. Bergey’s Manual of Systematic Bacteriology Springer Science & Business Media, USA.
  • Łubkowska, B., Jeżewska-Frąckowiak, J., Sroczyński, M., Dzitkowska-Zabielska, M., Bojarczuk, A., Skowron, P. M. and Cięszczyk, P. (2023). Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements. Microorganisms, 11(2): 488.
  • McClung, L. S. and Toabe, R. (1947). The Egg Yolk Plate Reaction for the Presumptive Diagnosis of Clostridium sporogenes and Certain Species of the Gangrene and Botulinum Groups. Journal of Bacteriology, 53(2): 139-147.
  • Mohd Isa, M. H., Shamsudin, N. H., Al-Shorgani, N. K. N., Alsharjabi, F. A. and Kalil, M. S. (2020). Evaluation of antibacterial potential of biosurfactant produced by surfactin-producing Bacillus isolated from selected Malaysian fermented foods. Food Biotechnology, 34(1): 1-24.
  • Mondal, H., Thomas, J. and Amaresan, N. (2023) Assay of hemolytic activity. In: Aquaculture Microbiology New York, NY: Springer US, pp. 187–189.
  • Nithya, V. and Halami, P. M. (2013). Evaluation of the probiotic characteristics of Bacillus species isolated from different food sources. Annals of Microbiology, 63(1): 129-137.
  • Nwagu, T. N., Ugwuodo, C. J., Onwosi, C. O., Inyima, O., Uchendu, O. C. and Akpuru, C. (2020). Evaluation of the probiotic attributes of Bacillus strains isolated from traditional fermented African locust bean seeds (Parkia biglobosa),“daddawa”. Annals of Microbiology, 70(1): 1-15.
  • Obi, S. K. C. (1980). Lecithinase and Toxin Production in Bacillus Species. Zentralblatt Für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten Und Parasitologie, 246(3): 415-422.
  • Pahumunto, N., Dahlen, G., Teanpaisan, R. (2021). Evaluation of potential probiotic properties of Lactobacillus and Bacillus strains derived from various sources for their potential use in swine feeding. Probiotics and Antimicrobial Proteins, 15(3): 479-490.
  • Patel, A. K., Ahire, J. J., Pawar, S. P., Chaudhari, B. L. and Chincholkar, S. B. (2009). Comparative accounts of probiotic characteristics of Bacillus spp. isolated from food wastes. Food Research International, 42(4): 505-510.
  • Satapute, P., Olekar, H. S., Shetti, A., Kulkarni, A., Hiremath, G., Patagundi, B. I., … Kaliwal, B. B. (2012). Isolation and characterization of nitrogen fixing Bacillus subtilis strain as-4 from agricultural soil. International Journal of Recent Scientific Research, 3: 762-765.
  • Senesi, S., Celandroni, F., Tavanti, A. and Ghelardi, E. (2001). Molecular Characterization and Identification of Bacillus clausii Strains Marketed for Use in Oral Bacteriotherapy. Applied and Environmental Microbiology, 67(2): 834-839.
  • Sharma, P., Das, S., Sadhu, P., Pal, S., Mitra, S., Ghoshal, A., ... and Sarkar, S. (2023). Therapeutic role of probiotics in managing various diseases. Journal of Survey in Fisheries Sciences, 10(1S): 6378-6380.
  • Sharma, P., Tomar, S. K., Goswami, P., Sangwan, V. and Singh, R. (2014). Antibiotic resistance among commercially available probiotics. Food Research International, 57: 176-195.
  • Smith, P. B. Y. (1981). Biochemical Tests for Identification of Medical Bacteria. Second Edition. International Journal of Systematic and Evolutionary Microbiology, 31(1): 108-108.
  • Sorokulova, I. B., Pinchuk, I. V., Denayrolles, M., Osipova, I. G., Huang, J. M., Cutting, S. M. and Urdaci, M. C. (2008). The safety of two Bacillus probiotic strains for human use. Digestive Diseases and Sciences, 53(4): 954-963.
  • Soylu, S., Kara M., Soylu, E. M., Uysal, A. and Kurt, Ş. (2022). Determination of Biocontrol Potentials of Endophytic Bacteria in Biological Control of Citrus Sour Rot Disease Caused by Geotrichum citri-aurantii. Journal of Agricultural Tekirdağ Faculty, 19(1): 177-191.
  • Sui, L., Zhu, X., Wu, D., Ma, T., Tuo, Y., Jiang, S., … and Mu, G. (2020). In vitro assessment of probiotic and functional properties of Bacillus coagulans T242. Food Bioscience, 36: 100675.
  • Thankappan, B., Ramesh, D., Ramkumar, S., Natarajaseenivasan, K. and Anbarasu, K. (2015). Characterization of Bacillus spp. from the gastrointestinal tract of labeo rohita—towards to identify novel probiotics against fish pathogens. Applied Biochemistry and Biotechnology, 175(1): 340-353.
  • Urdaci, M. C., Bressollier, P. and Pinchuk, I. (2004). Bacillus clausii probiotic strains: antimicrobial and immunomodulatory activities. Journal of Clinical Gastroenterology, 38(6 Suppl): S86-90.
  • Vlková, E., Rada, V., Šmehilová, M. and Killer, J. (2008). Auto-aggregation and Co-aggregation ability in bifido bacteria and clostridia. Folia Microbiologica, 53(3): 263-269.
  • Zeng, Z., He, X., Li, F., Zhang, Y., Huang, Z., Wang, Y. and Li, J. (2021). Probiotic properties of Bacillus proteolyticus isolated from Tibetan yaks, China. Frontiers in Microbiology, 12: 649207.
  • Zeng, Z., Zhang, J., Li, Y. et al. (2022). Probiotic potential of Bacillus licheniformis and Bacillus pumilus isolated from Tibetan yaks, China. Probiotics and Antimicrobial Proteins, 14: 579–594.

Çeşitli Kaynaklardan ve Ticari Gıda Takviyelerinden İzole Edilen Bacillus Türlerinin Bazı Probiyotik Özelliklerinin Karşılaştırmalı Değerlendirilmesi

Year 2024, Volume: 21 Issue: 1, 265 - 276, 30.01.2024

Burcu Kahraman Burcu Mine Şenol Enes Dertli Muhammet Arıcı

https://doi.org/10.33462/jotaf.1329533

Abstract

Bacillus türleri gram pozitif, aerobik, peritrik olarak kamçılı ve endospor oluşturan bakterilerdir. Çevrede her yerde; özellikle toprak (yaygın habitatı), su, toz veya havada bulunabilirler. Bacillus türleri içerisinde önemli bir grubu ise sağlığa faydalı etki gösteren probiyotikler oluşturmaktadır. Probiyotik Bacillus'ları içeren gıdalar ve yemler, genellikle; insanlar için besin takviyesi, hayvanlar için büyümeyi teşvik edici, su ürünleri için ise büyüme düzenleyici veya hastalıklara karşı direnç sağlayıcı olarak kullanılmaktadır. Bu çalışmada, çeşitli kaynaklardan probiyotik Bacillus izolasyonu, moleküler tanımlanması ve probiyotik özelliklerinin belirlenmesi amaçlanmıştır. Bu amaçla, balık bağırsağından, topraktan, olgunlaştırılmış peynirden ve ticari probiyotik gıda katkılarından elde edilen 58 Bacillus izolatından 8 adet Bacillus subtilis, Bacillus coagulans ve Bacillus clausii suşu tanımlanmış ve probiyotik özellikleri karakterize edilmiştir. İlk olarak, Bacillus suşları 16S rRNA PCR analizi ile moleküler olarak tanımlanmıştır. Bacillus cinsine ait izolatların fizyolojik ve biyokimyasal özelliklerini belirlemek için farklı sıcaklıklarda, tuz konsantrasyonlarında ve pH derecelerinde büyümeleri incelenmiş, ardından eskülin hidrolizi, nişasta hidrolizi, nitrat redüksiyonu, glikozdan gaz oluşumu testleri yapılmıştır. Bacillus izolatlarının probiyotik potansiyelinin değerlendirilmesi açısından; safra tuzu toleransı, hücre yüzeyi hidrofobikliği, oto-agregasyon, antibiyotik duyarlılık testleri yapılmıştır. Tüm analizlerde, gıda takviyelerinden elde edilen suşlar yüksek düzeyde hidrofobiklik ve oto-agregasyon özellikleri göstermiştir ve bu suşları takip eden en yüksek değerler sırasıyla balık bağırsağından ve topraktan izole edilen Bacillus subtilis suşlarında (F1 ve S2) gözlemlenmiştir. Tüm suşlar, safra tuzu koşullarında güçlü gelişme özellikleri göstermiştir. Bacillus suşlarının antibiyotik duyarlılığını suşa özgü özellikler belirlemiştir. tetrasikline karşı yüksek düzeyde duyarlılık gözlenmiştir. Özetle, bu çalışma çeşitli kaynaklardan izole edilen Bacillus suşlarının potansiyel probiyotik özelliklerini ortaya koymuş ve bu probiyotik özellikler gıda takviyelerinden izole edilen Bacillus suşları ile karşılaştırılmıştır.

Keywords

Bacillus Moleküler tanımlama Biyokimyasal karakterizasyon Probiyotikler

Project Number

5190060

References

  • Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3): 403-410.
  • Baker, G. C., Smith, J. J. and Cowan, D. A. (2003). Review and re-analysis of domain-specific 16S primers. Journal of Microbiological Methods, 55(3): 541-555.
  • Banerjee, G., Nandi, A. and Ray, A. K. (2017). Assessment of hemolytic activity, enzyme production and bacteriocin characterization of Bacillus subtilis LR1 isolated from the gastrointestinal tract of fish. Archives of Microbiology, 199: 115–124.
  • Bezkorovainy, A. (2001). Probiotics: determinants of survival and growth in the gut. The American Journal of Clinical Nutrition, 73(2 Suppl): 399-405.
  • Chaiyawan, N., Taveeteptaikul, P., Wannissorn, B., Ruengsomwong, S., Klungsupya, P., Buaban, W. and Itsaranuwat, P. (2010). Characterization and Probiotic Properties of Bacillus Strains Isolated from Broiler. The Thai Journal of Veterinary Medicine, 40(2): 207-214.
  • Conway, P. L., Gorbach, S. L., Goldin, B. R. (1987). Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. Journal of Dairy Science, 70(1): 1-12.
  • Cutting, S. M. (2011). Bacillus probiotics. Food Microbiology, 28(2): 214-220.
  • Dabiré, Y., Somda, N. S., Somda, M. K., Compaoré, C. B., Mogmenga, I., Ezeogu, L. I., ... and Dicko, M. H. (2022). Assessment of probiotic and technological properties of Bacillus spp. isolated from Burkinabe Soumbala. BMC Microbiology, 22(1): 1-13.
  • Endres, J. R., Clewell, A., Jade, K. A., Farber, T., Hauswirth, J., Schauss, A. G. (2009). Safety assessment of a proprietary preparation of a novel Probiotic, Bacillus coagulans, as a food ingredient. Food and Chemical Toxicology, 47(6): 1231-1238.
  • Freitas, D. B., Reis, M. P., Lima-Bittencourt, C. I., Costa, P. S., Assis, P. S., Chartone-Souza, E. and Nascimento, A. M. (2008). Genotypic and phenotypic diversity of Bacillus spp. isolated from steel plant waste. BMC Research Notes, 1(1): 92.
  • Gatson, J. W., Benz, B. F., Chandrasekaran, C., Satomi, M., Venkateswaran, K. and Hart, M. E. (2006). Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis. International Journal of Systematic and Evolutionary Microbiology, 56(7): 1475-1484.
  • Ghosh, K., Sen, S. K. and Ray, A. K. (2002). Characterization of Bacilli isolated from the gut of rohu, Labeo rohita, fingerlings and its significance in digestion. Journal of Applied Aquaculture, 12(3): 33-42.
  • Giri, S. S., Sukumaran, V. and Dangi, N. K. (2012). Probiotics and Antimicrobial Proteins, 4(4): 238–242.
  • Green, D. H., Wakeley, P. R., Page, A., Barnes, A., Baccigalupi, L., Ricca, E. and Cutting, S. M. (1999).
  • Characterization of two Bacillus probiotics. Applied and Environmental Microbiology, 65(9): 4288-4291.
  • Güldoğan, Ö., Aktepe, B. P. and Aysan, Y. (2022) Use of different bacillus species in the biological control of tomato bacterial speck disease. Journal of Tekirdag Agricultural Faculty, 19(4): 829-839.
  • Hoa, N. T., Baccigalupi, L., Huxham, A., Smertenko, A., Van, P. H., Ammendola, S., …and Cutting, S. M. (2000). Characterization of Bacillus Species Used for Oral Bacteriotherapy and Bacterioprophylaxis of Gastrointestinal Disorders. Applied and Environmental Microbiology, 66(12): 5241-5247.
  • Hong, H. A., To, E., Fakhry, S., Baccigalupi, L., Ricca, E. and Cutting, S. M. (2009). Defining the natural habitat of Bacillus spore-formers". Research in Microbiology, 160(6): 375-379.
  • İspirli, H., Demirbaş, F. and Dertli, E. (2015). Characterization of functional properties of Enterococcus faecium strains isolated from human gut. Canadian Journal of Microbiology, 61(11): 861-870.
  • Joint FAO/WHO Working Group (2002) Report on Drafting Guidelines for the Evaluation of Probiotics in Food: Guidelines for the Evaluation of Probiotics in Food, London, Ontorio, Canada 30 April-1 May 2002.
  • Kavitha, M., Raja, M. and Perumal, P. (2018). Evaluation of probiotic potential of Bacillus spp. isolated from the digestive tract of freshwater fish Labeo calbasu. Aquaculture Reports, 11: 59-69.
  • Kos, B., Šušković, J., Vuković, S., Šimpraga, M., Frece, J. and Matošić, S. (2003). Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. Journal of Applied Microbiology, 94(6): 981-987.
  • Lei, J., Ran, X., Guo, M., Liu, J., Yang, F. and Chen, D. (2023). Screening, identification, and probiotic properties of Bacillus pumilus from Yak. Probiotics and Antimicrobial Proteins, https://doi.org/10.1007/s12602-023-10054-w
  • Ljungh, A. and Wadström, T. (2006). Lactic acid bacteria as probiotics. Current Issues in Intestinal Microbiology, 7(2): 73-89.
  • Logan, N A., De Vos, P. A. U. L. (2009). Family Bacillaceae. Bergey’s Manual of Systematic Bacteriology Springer Science & Business Media, USA.
  • Łubkowska, B., Jeżewska-Frąckowiak, J., Sroczyński, M., Dzitkowska-Zabielska, M., Bojarczuk, A., Skowron, P. M. and Cięszczyk, P. (2023). Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements. Microorganisms, 11(2): 488.
  • McClung, L. S. and Toabe, R. (1947). The Egg Yolk Plate Reaction for the Presumptive Diagnosis of Clostridium sporogenes and Certain Species of the Gangrene and Botulinum Groups. Journal of Bacteriology, 53(2): 139-147.
  • Mohd Isa, M. H., Shamsudin, N. H., Al-Shorgani, N. K. N., Alsharjabi, F. A. and Kalil, M. S. (2020). Evaluation of antibacterial potential of biosurfactant produced by surfactin-producing Bacillus isolated from selected Malaysian fermented foods. Food Biotechnology, 34(1): 1-24.
  • Mondal, H., Thomas, J. and Amaresan, N. (2023) Assay of hemolytic activity. In: Aquaculture Microbiology New York, NY: Springer US, pp. 187–189.
  • Nithya, V. and Halami, P. M. (2013). Evaluation of the probiotic characteristics of Bacillus species isolated from different food sources. Annals of Microbiology, 63(1): 129-137.
  • Nwagu, T. N., Ugwuodo, C. J., Onwosi, C. O., Inyima, O., Uchendu, O. C. and Akpuru, C. (2020). Evaluation of the probiotic attributes of Bacillus strains isolated from traditional fermented African locust bean seeds (Parkia biglobosa),“daddawa”. Annals of Microbiology, 70(1): 1-15.
  • Obi, S. K. C. (1980). Lecithinase and Toxin Production in Bacillus Species. Zentralblatt Für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten Und Parasitologie, 246(3): 415-422.
  • Pahumunto, N., Dahlen, G., Teanpaisan, R. (2021). Evaluation of potential probiotic properties of Lactobacillus and Bacillus strains derived from various sources for their potential use in swine feeding. Probiotics and Antimicrobial Proteins, 15(3): 479-490.
  • Patel, A. K., Ahire, J. J., Pawar, S. P., Chaudhari, B. L. and Chincholkar, S. B. (2009). Comparative accounts of probiotic characteristics of Bacillus spp. isolated from food wastes. Food Research International, 42(4): 505-510.
  • Satapute, P., Olekar, H. S., Shetti, A., Kulkarni, A., Hiremath, G., Patagundi, B. I., … Kaliwal, B. B. (2012). Isolation and characterization of nitrogen fixing Bacillus subtilis strain as-4 from agricultural soil. International Journal of Recent Scientific Research, 3: 762-765.
  • Senesi, S., Celandroni, F., Tavanti, A. and Ghelardi, E. (2001). Molecular Characterization and Identification of Bacillus clausii Strains Marketed for Use in Oral Bacteriotherapy. Applied and Environmental Microbiology, 67(2): 834-839.
  • Sharma, P., Das, S., Sadhu, P., Pal, S., Mitra, S., Ghoshal, A., ... and Sarkar, S. (2023). Therapeutic role of probiotics in managing various diseases. Journal of Survey in Fisheries Sciences, 10(1S): 6378-6380.
  • Sharma, P., Tomar, S. K., Goswami, P., Sangwan, V. and Singh, R. (2014). Antibiotic resistance among commercially available probiotics. Food Research International, 57: 176-195.
  • Smith, P. B. Y. (1981). Biochemical Tests for Identification of Medical Bacteria. Second Edition. International Journal of Systematic and Evolutionary Microbiology, 31(1): 108-108.
  • Sorokulova, I. B., Pinchuk, I. V., Denayrolles, M., Osipova, I. G., Huang, J. M., Cutting, S. M. and Urdaci, M. C. (2008). The safety of two Bacillus probiotic strains for human use. Digestive Diseases and Sciences, 53(4): 954-963.
  • Soylu, S., Kara M., Soylu, E. M., Uysal, A. and Kurt, Ş. (2022). Determination of Biocontrol Potentials of Endophytic Bacteria in Biological Control of Citrus Sour Rot Disease Caused by Geotrichum citri-aurantii. Journal of Agricultural Tekirdağ Faculty, 19(1): 177-191.
  • Sui, L., Zhu, X., Wu, D., Ma, T., Tuo, Y., Jiang, S., … and Mu, G. (2020). In vitro assessment of probiotic and functional properties of Bacillus coagulans T242. Food Bioscience, 36: 100675.
  • Thankappan, B., Ramesh, D., Ramkumar, S., Natarajaseenivasan, K. and Anbarasu, K. (2015). Characterization of Bacillus spp. from the gastrointestinal tract of labeo rohita—towards to identify novel probiotics against fish pathogens. Applied Biochemistry and Biotechnology, 175(1): 340-353.
  • Urdaci, M. C., Bressollier, P. and Pinchuk, I. (2004). Bacillus clausii probiotic strains: antimicrobial and immunomodulatory activities. Journal of Clinical Gastroenterology, 38(6 Suppl): S86-90.
  • Vlková, E., Rada, V., Šmehilová, M. and Killer, J. (2008). Auto-aggregation and Co-aggregation ability in bifido bacteria and clostridia. Folia Microbiologica, 53(3): 263-269.
  • Zeng, Z., He, X., Li, F., Zhang, Y., Huang, Z., Wang, Y. and Li, J. (2021). Probiotic properties of Bacillus proteolyticus isolated from Tibetan yaks, China. Frontiers in Microbiology, 12: 649207.
  • Zeng, Z., Zhang, J., Li, Y. et al. (2022). Probiotic potential of Bacillus licheniformis and Bacillus pumilus isolated from Tibetan yaks, China. Probiotics and Antimicrobial Proteins, 14: 579–594.
There are 47 citations in total.

Details

Primary Language English
Subjects Food Microbiology
Journal Section Articles
Authors

Burcu Kahraman 0000-0002-7553-4950

Burcu Mine Şenol 0000-0003-2779-0789

Enes Dertli 0000-0002-0421-6103

Muhammet Arıcı 0000-0003-4126-200X

Project Number 5190060
Early Pub Date January 24, 2024
Publication Date January 30, 2024
Submission Date August 7, 2023
Acceptance Date December 9, 2023
Published in Issue Year 2024 Volume: 21 Issue: 1

Cite

APA Kahraman, B., Şenol, B. M., Dertli, E., Arıcı, M. (2024). A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties. Tekirdağ Ziraat Fakültesi Dergisi, 21(1), 265-276. https://doi.org/10.33462/jotaf.1329533
AMA Kahraman B, Şenol BM, Dertli E, Arıcı M. A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties. JOTAF. January 2024;21(1):265-276. doi:10.33462/jotaf.1329533
Chicago Kahraman, Burcu, Burcu Mine Şenol, Enes Dertli, and Muhammet Arıcı. “A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties”. Tekirdağ Ziraat Fakültesi Dergisi 21, no. 1 (January 2024): 265-76. https://doi.org/10.33462/jotaf.1329533.
EndNote Kahraman B, Şenol BM, Dertli E, Arıcı M (January 1, 2024) A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties. Tekirdağ Ziraat Fakültesi Dergisi 21 1 265–276.
IEEE B. Kahraman, B. M. Şenol, E. Dertli, and M. Arıcı, “A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties”, JOTAF, vol. 21, no. 1, pp. 265–276, 2024, doi: 10.33462/jotaf.1329533.
ISNAD Kahraman, Burcu et al. “A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties”. Tekirdağ Ziraat Fakültesi Dergisi 21/1 (January 2024), 265-276. https://doi.org/10.33462/jotaf.1329533.
JAMA Kahraman B, Şenol BM, Dertli E, Arıcı M. A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties. JOTAF. 2024;21:265–276.
MLA Kahraman, Burcu et al. “A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties”. Tekirdağ Ziraat Fakültesi Dergisi, vol. 21, no. 1, 2024, pp. 265-76, doi:10.33462/jotaf.1329533.
Vancouver Kahraman B, Şenol BM, Dertli E, Arıcı M. A Comparative Study of Bacillus Spp. Isolated from Various Sources and Commercial Food Supplements and Evaluation of Some Probiotic Properties. JOTAF. 2024;21(1):265-76.
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