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Ratlarda postoperatif peritoneal adezyonların önlenmesinde saf zeytinyağının uzun dönem etkinliği

Year 2019, Volume: 3 Issue: 3, 218 - 222, 15.03.2019

Dilan Altıntaş Ural Haluk Saruhan İsmail Saygın Duygun Altıntaş Aykan Alper Ural Mustafa İmamoğlu

https://doi.org/10.28982/josam.465600

Abstract

Amaç: Abdominopelvik cerrahi sonrası ortaya çıkan postoperatif peritoneal adezyonlar (PPA) cerrahların güncel sorunlarındandır. Bu çalışmanın amacı, saf zeytinyağının PPA önlenmesindeki etkisini araştırmaktır.

Yöntemler: 32 rat randomize dört gruba ayrıldı: (1) Sham grubu: Laparotomisiz peritoneal kaviteye perkütan olarak 5 ml saf zeytinyağı enjekte edildi, (2) Adezyon grubu: Jejunum, ileum ve çekum üzerine standart bir adezyon modeli oluşturuldu. (3) Adezyon + zeytinyağı: Jejunum, ileum ve çekumda standart bir adezyon modeli oluşturuldu. Daha sonra jejunum, ileum ve çekum üzerindeki alan 5 ml saf zeytinyağı ile kaplandı. (4) Zeytinyağı + adezyon: Jejunum, ileum ve çekum üzerindeki alan 5 ml saf zeytinyağı ile kaplandı. Daha sonra bu alanda standart adezyon modeli oluşturuldu. Dört hafta sonra, sıçanların karın boşlukları, PPA için Evans'ın adezyon sınıflandırması ve Zühlke'nin histopatolojik derecelenme ölçekleri kullanılarak incelendi.

Bulgular: Zeytinyağının intraperitoneal olarak enjekte edildiği Grup 1’de herhangi bir adezyon bulunmadı. Grup 2’deki tüm ratlarda PPA mevcuttu. Grup 3 ve 4’ün mikroskopik adezyon skorları Grup 2’den istatistiksel olarak anlamlı düzeyde daha azdı. Kollajen lif fraksiyonları belirgin şekilde daha düşüktü ve fibrozisde belirgin bir azalma gözlendi. Grup 3, Grup 4 ile karşılaştırıldığında ise, mikroskopik adezyon skoru açısından istatistiksel olarak anlamlı fark yoktu.

Sonuç: Saf zeytinyağının antiinflamasyon, doku rejenerasyonu ve hidroflotasyon etkisinin, yaralanmış peritoneal yüzey üzerinde uzun süreli etki sağlayarak ratlarda PPA oluşumunu azalttığını bulduk. Bu nedenle, ucuz ve satın alınması kolay bir ürün olan zeytinyağı, cerrahi kliniklerinde PPA vakalarında kullanılabilir.

Keywords

Postoperatif peritoneal adezyon Zeytinyağı Cerrahi

References

  • 1. Liakakos T, Thomakos N, Fine PM, Dervenis C, Young RL. Peritoneal adhesions: etiology, pathophysiology, and clinical significance. Recent advances in prevention and management. Dig Surg. 2001;18(4):260-73.
  • 2. Menzies D. Postoperative adhesions: their treatment and relevance in clinical practice. Ann R Coll Surg Engl. 1993;75(3):147-53.
  • 3. Tolu A, Gökçe Ö. Adezyonların Sebepleri ve Önlenmesi. T Klin Tıp Bilimleri. 1992;12(3):244-9.
  • 4. Arnold PB, Green CW, Foresman PA, Rodeheaver GT. Evaluation of resorbable barriers for preventing surgical adhesions. Fertil Steril. 2000;73(1):157-61.
  • 5. Kırdak T, Uysal E, Korun N. Karın içi yapışıklıkların önlenmesinde metilprednizolonun farklı dozlarının etkinliğinin incelenmesi. Ulus Travma Acil Cerrahi Derg. 2008;14(3):188-91.
  • 6. Ryan GB, Grobety J, Majno G. Postoperative peritoneal adhesions. A study of the mechanisms. Am J Pathol. 1971;65(1):117-48.
  • 7. Gomel V, Urman B, Gurgan T. Pathophysiology of adhesion formation and strategies for prevention. J Reprod Med. 1996;41(1):35-41.
  • 8. Speroni E, Guerra MC, Minghetti A, Crespi-Perellino N, Pasini P, Piazza F, et.al. Oleuropein evaluated in vitro and in vivo as an antioxidant. Phytother. Res., 1998;12:98-100.
  • 9. De la Puerta R, Martinez Dominguez E, Ruiz-Gutierrez V. Effect of minor components of virgin olive oil on topical antiinflammatory assays. Z Naturforsch. 2000;55:814-9.
  • 10. Tranter HS, Tassou SC, Nychas GJ. The effect of the olive phenolic compound, oleuropein, on growth and enterotoxin B production by Staphylococcus aureus. J. Appl.Bacteriol. 1993;74:253-9.
  • 11. Evans DM, McAree K, Guyton DP. Dose dependency and wound healing aspects of the use of tissue plasminogen activator in the prevention of intra-abdominal adhesions. Am J Surg. 1993;165(6):229-32.
  • 12. Zühlke HV, Lorenz EM, Straub EM, Savvas V. Pathophysiology and classification of adhesions. Langenbecks Arch Chir Suppl II Verh Dtsch Ges Chir 1990:1009-16.
  • 13. Audebert AJ, Gomel V. Role of microlaparoscopy in the diagnosis of peritoneal and visceral adhesions and in the prevention of bowel injury associated with blind trocar insertion. Fertil Steril. 2000;73(3):631-5.
  • 14. Millamiemi H, Frolander M. The effect of glove powders and their constituents on adhesions and granuloma formation in the abdominal cavity of the rabbit. ACTA Chir Scand. 1966;131:312-8.
  • 15. Blauer KL, Collins RL. The effect of intraperitoneal progesterone on postoperative adhesion formation in rabbit. Fertil Steril. 1988;49:144-9.
  • 16. Drollette CM, Badawy SZA. Pathophysiology of pelvic adhesions: modern trends in preventing infertility. J Reprod Med. 1992;37:107-21.
  • 17. Ryan G, Grobety J, Majino G. Postoperative peritoneal adhesions: a study of mechanism. Am J Pathol. 1971;65:117-48.
  • 18. Demirel H, Altay K, Sultanoğlu E, Dolgun A, Odabaş Ö. Postoperatif İntraperitoneal Adezyonların Proflaksisinde Aprotinin, Dextran 7 Ve %1'luk Yağ Emülsiyonu ile Karşılaştırmalı Bir Çalışma. Türkiye Klinikleri Tıp Bilimleri Araştırma Degisi. 1990;8(6):524-8.
  • 19. Tolu A, Gökçe Ö. Adezyonların Sebepleri ve Önlenmesi. T Klin Tıp Bilimleri. 1992;12(3):244-9.
  • 20. Galili Y, Ben-Abraham R, Rabau M, Klausner J, Kluger Y. Reduction of surgery-induced peritoneal adhesions by methylene blue. Am J Surg. 1998;175(1):30-2.
  • 21. De la Portilla F, Ynfante I, Bejarano D, Conde J, Fernandez A, Ortega JM, et al. Prevention of peritoneal adhesions by intraperitoneal administration of vitamin E: an experimental study in rats. Dis Colon Rectum. 2004;47(12):2157-61.
  • 22. Dargenio R, Cimino C, Ragusa G, Garcea N, Stella C. Pharmacological prevention of postoperative adhesions experimentally induced in the rat. Acta Eur Fertil. 1986 Jul-Aug;17(4):267-72.
  • 23. Özçelik A, Yurdakul İ. İntraabdominal Adezyonlar ve Önlenmesi. Veteriner Cerrahi Dergisi. 2006;12(1-4):62-7.
  • 24. Holmdahl L, Eriksson E, Eriksson BI, Risberg B. Depression of peritoneal fibrinolysis during operation is a local response to trauma. Surgery. 1998;123(5):539-44.
  • 25. Cicerale S, Lucas L, Keast R. Biological activities of phenolic compounds present in virgin olive oil. Int J Mol Sci. 2010 Feb 2;11(2):458-79.
  • 26. Ellis H. The magnitude of adhesion-related problems. In: M.D. GSd, eds. Peritoneal Surgery. New York: Springer; 2000. pp. 297-306.
  • 27. Baum C, Arpey C. Normal cutaneous wound healing: Clinical correlation with cellular and molecular events. Dermatol Surg. 2005;31(6):674-8661.

Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats

Year 2019, Volume: 3 Issue: 3, 218 - 222, 15.03.2019

Dilan Altıntaş Ural Haluk Saruhan İsmail Saygın Duygun Altıntaş Aykan Alper Ural Mustafa İmamoğlu

https://doi.org/10.28982/josam.465600

Abstract

Aim: Postoperative peritoneal adhesion (PPA) that occur after abdominopelvic surgery are the current problems of surgeons. The aim of this study was to investigate the effect of pure olive oil in preventing PPA.

Methods: Thirty-two rats were randomly divided into four groups: (1) Sham group: 5 ml pure olive oil was injected percutaneously into the peritoneal cavity without laparotomy, (2) Adhesion group: A standard adhesion model was formed on jejunum, ileum and caecum. (3) Adhesion + olive oil: A standard adhesion model was formed on jejunum, ileum and caecum. Subsequently the area on jejunum, ileum and caecum was covered with 5 ml of pure olive oil. (4) Olive oil + adhesion: The area on jejunum, ileum and caecum was covered with 5 ml of pure olive oil. Subsequently the standard adhesion model was formed on this area. Four weeks later, abdominal cavities of rats were examined for PPA, using Evan’s adhesion classification and Zühlke’s histopathological grade scales.

Results: PPA was not found in Group 1 in which olive oil was injected intraperitoneally. PPA was present in all rats in Group 2. Microscopic adhesion scores in Group 3 and 4 were significantly lower than Group 2. The collagen fiber fractions were significantly lower and there was a significant decrease in fibrosis. There was no statistically significant difference in microscopic adhesion scores in Group 3 compared to Group 4.

Conclusion: We found that antiinflammation, tissue regeneration and hydroflotation effect of pure olive oil decreased PPA formation in rats by maintaining a long lasting effect on the wounded peritoneal surface. Thus olive oil, a cheap and easy to obtain product, can be used in cases of PPA in surgery clinics.

Keywords

Postoperative peritoneal adhesions Olive oil Surgery

References

  • 1. Liakakos T, Thomakos N, Fine PM, Dervenis C, Young RL. Peritoneal adhesions: etiology, pathophysiology, and clinical significance. Recent advances in prevention and management. Dig Surg. 2001;18(4):260-73.
  • 2. Menzies D. Postoperative adhesions: their treatment and relevance in clinical practice. Ann R Coll Surg Engl. 1993;75(3):147-53.
  • 3. Tolu A, Gökçe Ö. Adezyonların Sebepleri ve Önlenmesi. T Klin Tıp Bilimleri. 1992;12(3):244-9.
  • 4. Arnold PB, Green CW, Foresman PA, Rodeheaver GT. Evaluation of resorbable barriers for preventing surgical adhesions. Fertil Steril. 2000;73(1):157-61.
  • 5. Kırdak T, Uysal E, Korun N. Karın içi yapışıklıkların önlenmesinde metilprednizolonun farklı dozlarının etkinliğinin incelenmesi. Ulus Travma Acil Cerrahi Derg. 2008;14(3):188-91.
  • 6. Ryan GB, Grobety J, Majno G. Postoperative peritoneal adhesions. A study of the mechanisms. Am J Pathol. 1971;65(1):117-48.
  • 7. Gomel V, Urman B, Gurgan T. Pathophysiology of adhesion formation and strategies for prevention. J Reprod Med. 1996;41(1):35-41.
  • 8. Speroni E, Guerra MC, Minghetti A, Crespi-Perellino N, Pasini P, Piazza F, et.al. Oleuropein evaluated in vitro and in vivo as an antioxidant. Phytother. Res., 1998;12:98-100.
  • 9. De la Puerta R, Martinez Dominguez E, Ruiz-Gutierrez V. Effect of minor components of virgin olive oil on topical antiinflammatory assays. Z Naturforsch. 2000;55:814-9.
  • 10. Tranter HS, Tassou SC, Nychas GJ. The effect of the olive phenolic compound, oleuropein, on growth and enterotoxin B production by Staphylococcus aureus. J. Appl.Bacteriol. 1993;74:253-9.
  • 11. Evans DM, McAree K, Guyton DP. Dose dependency and wound healing aspects of the use of tissue plasminogen activator in the prevention of intra-abdominal adhesions. Am J Surg. 1993;165(6):229-32.
  • 12. Zühlke HV, Lorenz EM, Straub EM, Savvas V. Pathophysiology and classification of adhesions. Langenbecks Arch Chir Suppl II Verh Dtsch Ges Chir 1990:1009-16.
  • 13. Audebert AJ, Gomel V. Role of microlaparoscopy in the diagnosis of peritoneal and visceral adhesions and in the prevention of bowel injury associated with blind trocar insertion. Fertil Steril. 2000;73(3):631-5.
  • 14. Millamiemi H, Frolander M. The effect of glove powders and their constituents on adhesions and granuloma formation in the abdominal cavity of the rabbit. ACTA Chir Scand. 1966;131:312-8.
  • 15. Blauer KL, Collins RL. The effect of intraperitoneal progesterone on postoperative adhesion formation in rabbit. Fertil Steril. 1988;49:144-9.
  • 16. Drollette CM, Badawy SZA. Pathophysiology of pelvic adhesions: modern trends in preventing infertility. J Reprod Med. 1992;37:107-21.
  • 17. Ryan G, Grobety J, Majino G. Postoperative peritoneal adhesions: a study of mechanism. Am J Pathol. 1971;65:117-48.
  • 18. Demirel H, Altay K, Sultanoğlu E, Dolgun A, Odabaş Ö. Postoperatif İntraperitoneal Adezyonların Proflaksisinde Aprotinin, Dextran 7 Ve %1'luk Yağ Emülsiyonu ile Karşılaştırmalı Bir Çalışma. Türkiye Klinikleri Tıp Bilimleri Araştırma Degisi. 1990;8(6):524-8.
  • 19. Tolu A, Gökçe Ö. Adezyonların Sebepleri ve Önlenmesi. T Klin Tıp Bilimleri. 1992;12(3):244-9.
  • 20. Galili Y, Ben-Abraham R, Rabau M, Klausner J, Kluger Y. Reduction of surgery-induced peritoneal adhesions by methylene blue. Am J Surg. 1998;175(1):30-2.
  • 21. De la Portilla F, Ynfante I, Bejarano D, Conde J, Fernandez A, Ortega JM, et al. Prevention of peritoneal adhesions by intraperitoneal administration of vitamin E: an experimental study in rats. Dis Colon Rectum. 2004;47(12):2157-61.
  • 22. Dargenio R, Cimino C, Ragusa G, Garcea N, Stella C. Pharmacological prevention of postoperative adhesions experimentally induced in the rat. Acta Eur Fertil. 1986 Jul-Aug;17(4):267-72.
  • 23. Özçelik A, Yurdakul İ. İntraabdominal Adezyonlar ve Önlenmesi. Veteriner Cerrahi Dergisi. 2006;12(1-4):62-7.
  • 24. Holmdahl L, Eriksson E, Eriksson BI, Risberg B. Depression of peritoneal fibrinolysis during operation is a local response to trauma. Surgery. 1998;123(5):539-44.
  • 25. Cicerale S, Lucas L, Keast R. Biological activities of phenolic compounds present in virgin olive oil. Int J Mol Sci. 2010 Feb 2;11(2):458-79.
  • 26. Ellis H. The magnitude of adhesion-related problems. In: M.D. GSd, eds. Peritoneal Surgery. New York: Springer; 2000. pp. 297-306.
  • 27. Baum C, Arpey C. Normal cutaneous wound healing: Clinical correlation with cellular and molecular events. Dermatol Surg. 2005;31(6):674-8661.
There are 27 citations in total.

Details

Primary Language English
Subjects Surgery
Journal Section Research article
Authors

Dilan Altıntaş Ural 0000-0002-1976-9122

Haluk Saruhan This is me 0000-0002-0991-8235

İsmail Saygın 0000-0002-6013-6378

Duygun Altıntaş Aykan 0000-0001-8224-4006

Alper Ural This is me 0000-0001-8135-6444

Mustafa İmamoğlu This is me 0000-0001-8267-9755

Publication Date March 15, 2019
Published in Issue Year 2019 Volume: 3 Issue: 3

Cite

APA Altıntaş Ural, D., Saruhan, H., Saygın, İ., Altıntaş Aykan, D., et al. (2019). Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. Journal of Surgery and Medicine, 3(3), 218-222. https://doi.org/10.28982/josam.465600
AMA Altıntaş Ural D, Saruhan H, Saygın İ, Altıntaş Aykan D, Ural A, İmamoğlu M. Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. J Surg Med. March 2019;3(3):218-222. doi:10.28982/josam.465600
Chicago Altıntaş Ural, Dilan, Haluk Saruhan, İsmail Saygın, Duygun Altıntaş Aykan, Alper Ural, and Mustafa İmamoğlu. “Long-Term Outcomes of Pure Olive Oil to Prevent Postoperative Peritoneal Adhesions in Rats”. Journal of Surgery and Medicine 3, no. 3 (March 2019): 218-22. https://doi.org/10.28982/josam.465600.
EndNote Altıntaş Ural D, Saruhan H, Saygın İ, Altıntaş Aykan D, Ural A, İmamoğlu M (March 1, 2019) Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. Journal of Surgery and Medicine 3 3 218–222.
IEEE D. Altıntaş Ural, H. Saruhan, İ. Saygın, D. Altıntaş Aykan, A. Ural, and M. İmamoğlu, “Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats”, J Surg Med, vol. 3, no. 3, pp. 218–222, 2019, doi: 10.28982/josam.465600.
ISNAD Altıntaş Ural, Dilan et al. “Long-Term Outcomes of Pure Olive Oil to Prevent Postoperative Peritoneal Adhesions in Rats”. Journal of Surgery and Medicine 3/3 (March 2019), 218-222. https://doi.org/10.28982/josam.465600.
JAMA Altıntaş Ural D, Saruhan H, Saygın İ, Altıntaş Aykan D, Ural A, İmamoğlu M. Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. J Surg Med. 2019;3:218–222.
MLA Altıntaş Ural, Dilan et al. “Long-Term Outcomes of Pure Olive Oil to Prevent Postoperative Peritoneal Adhesions in Rats”. Journal of Surgery and Medicine, vol. 3, no. 3, 2019, pp. 218-22, doi:10.28982/josam.465600.
Vancouver Altıntaş Ural D, Saruhan H, Saygın İ, Altıntaş Aykan D, Ural A, İmamoğlu M. Long-term outcomes of pure olive oil to prevent postoperative peritoneal adhesions in rats. J Surg Med. 2019;3(3):218-22.
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