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Determination of Cephalaria tchihatchewii Boiss.'s Extracts Antioxidant Activity, Effect on Metabolic Enzymes, and Chemical Characterization by UPLC-MS/MS Analysis

Year 2023, Volume: 13 Issue: 4, 2655 - 2672, 01.12.2023
https://doi.org/10.21597/jist.1337439

Abstract

The present study aims to perform a comprehensive chemical characterization of the methanol and water extracts of Cephalaria tchihatchewii Boiss. (MECT, WECT) using UPLC-MS/MS, besides evaluating the potential antioxidant activity of these extracts and their effect on some metabolic enzymes. The antioxidant activity was assessed using various assays including DPPH, ABTS, DMPD, FRAP, CUPRAC, and Fe3+ reducing assays. The inhibition effects of the extracts against α-glycosidase, acetylcholinesterase and carbonic anhydrase II enzymes were evaluated. The IC50 values of MECT and WECT were for α-glycosidase were 28.98 and 34.19 μg/mL, for acetylcholinesterase were 21.82 and 26.52 μg/mL, and for carbonic anhydrase, 27.75 and 13.72 μg/mL, respectively. The amounts of phenolic compounds present in MECT and WECT were determined to be 47.00 and 46.00 μg GAE /mg extract, respectively. The amounts of flavonoits present in MECT and WECT were determined to be 60.98 and 49.34 μg KE/mg extract, respectively. The MECT and WECT exhibited distinct activities on DPPH, ABTS, and DMPD. The IC50 values were for DPPH 34.66 and 57.76 μg/mL for ABTS 20.39 and 17.33 µg/mL and for DMPD 53.32 and 57.76 g/mL, respectively. Reducing abilities of extracts were respectively Fe+3 reducing (λ700:0.377 and 0.680), FRAP (λ593:0.690 and 0.369), and CUPRAC (λ450:0.458 and 0.333). UPLC-MS/MS revealed that the major components are MECT (quinic acid 39.844 µg/mL, chlorogenic acid 38.412 µg/mL, fumaric acid 0.301 µg/mL), and WECT (chlorogenic acid 13.639 µg/mL, qunic acid 11.004 µg/mL, cyanidin-3-O-glucoside 0.778 µg/mL). The extracts were determined to have various biological activities, including antioxidant activity and α-glycosidase, acetylcholinesterase, carbonic anhydrase II enzyme inhibition effect.

References

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Cephalaria tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi ve UPLC-MS/MS Analizi ile Kimyasal Karakterizasyonun Belirlenmesi

Year 2023, Volume: 13 Issue: 4, 2655 - 2672, 01.12.2023
https://doi.org/10.21597/jist.1337439

Abstract

Bu çalışma, Cephalaria tchihatchewii Boiss'in metanol ve su ekstrelerinin (MECT, WECT) UPLC-MS/MS kullanarak kapsamlı bir kimyasal karakterizasyonunu gerçekleştirmeyi ve bu ekstrelerin potansiyel antioksidan aktivitesinin ve bazı metabolik enzimler üzerindeki etkisini değerlendirmeyi amaçlamaktadır. Antioksidan aktivite, DPPH, ABTS, DMPD, FRAP, KUPRAK ve Fe3+ indirgeme testleri dahil olmak üzere çeşitli testler kullanılarak değerlendirilmiştir. MECT ve WECT'in enzim inhibisyon IC50 değerleri, sırasıyla α-glikozidaz için 28.98 ve 34.19 μg/mL, asetilkolinesteraz için 21.82 ve 26.52 μg/mL ve karbonik anhidraz II için 27.75 ve 13.72 μg/mL’dir. MECT ve WECT'de bulunan fenolik bileşik miktarları sırasıyla 47.00 ve 46.00 μg GAE /mg ekstre, flavonoit miktarları 60.98 ve 49.34 μg KE/mg ekstre olarak belirlenmiştir. MECT ve WECT’in IC50 değerleri sırasıyla DPPH için 34.66 ve 57.76 μg/mL, ABTS için 20.39 ve 17.33 μg/mL ve DMPD için 53.32 ve 57.76 g/mL’dir. Ekstrelerin indirgeme yetenekleri ise sırasıyla Fe+3 indirgeme (λ700:0.377 ve 0.680), FRAP (λ593:0.690 ve 0.369) ve KUPRAK (λ450:0.458 ve 0.333)’dür. UPLC-MS/MS analiziyle elde edilen sonuçlara göre ana bileşenler MECT’in kinik asit 0.39844 µg/mL, klorojenik asit 38.412 µg/mL, fumarik asit 0.301 µg/mL ve WECT’in klorojenik asit 13.639 µg/mL, kinik asit 11.004 µg/mL, siyanidin-3-O-glukozit 0.778 µg/mL olduğu belirlenmiştir. Ekstrelerin, antioksidan aktivite ve α-glikosidaz, asetilkolinesteraz, karbonik anhidraz II enzim ihbisyon etkisi dahil olmak üzere çeşitli biyolojik aktivitelere sahip olduğu belirlenmiştir.

References

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  • Alam, M. N., Bristi, N. J., & Rafiquzzaman, M. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi pharmaceutical journal, 21(2), 143-152.
  • Apak, R., Güçlü, K., Özyürek, M., Esin Karademir, S., & Erçağ, E. (2006). The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International Journal of Food Sciences and Nutrition, 57(5-6), 292-304. https://doi.org/10.1080/09637480600798132
  • Arabaci, B., Gulcin, I., & Alwasel, S. (2014). Capsaicin: A Potent Inhibitor of Carbonic Anhydrase Isoenzymes. Molecules, 19(7), 10103-10114.
  • Atalan, E. (2019). Türkiye’de yetiştirilen pelemir bitkisinin [Cephalaria syriaca (l.)] antioksidan, antimikrobiyal, antifungal, antibiyofilm özelliklerinin ve tohum morfolojisinin araştırılması. Bartın Üniversitesi, Fen Bilimleri Enstitüsü.
  • Blois, M. S. (1958). Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181(4617), 1199-1200. https://doi.org/10.1038/1811199a0
  • Burak, B., Emel, M. K., & Yeter, Y. C. (2021). Total phenolic, total flavonoit contents, and in vitro biological activities of Cephalaria procera. İstanbul Journal of Pharmacy, 51(3), 365-371.
  • Celenk, V. U., Sarikahya, N. B., & Kirmizigul, S. (2020). Isolation and Structural Studies on Saponins from Three Cephalaria Species from Anatolia. Chemistry of Natural Compounds, 56(1), 180-182.
  • Chrząszcz, M., Miazga-Karska, M., Klimek, K., Granica, S., Tchórzewska, D., Ginalska, G., & Szewczyk, K. (2020a). Extracts from Cephalaria Uralensis (Murray) Roem. & Schult. and Cephalaria Gigantea (Ledeb.) Bobrov as Potential Agents for Treatment of Acne Vulgaris: Chemical Characterization and In Vitro Biological Evaluation. Antioxidants, 9, 796. doi:10.3390/antiox9090796
  • Chrząszcz, M., Miazga-Karska, M., Klimek, K., Granica, S., Tchórzewska, D., Ginalska, G., & Szewczyk, K. (2020b). Extracts from Cephalaria Uralensis (Murray) Roem. & Schult. and Cephalaria Gigantea (Ledeb.) Bobrov as Potential Agents for Treatment of Acne Vulgaris: Chemical Characterization and In Vitro Biological Evaluation. Antioxidants, 9(9), 796.
  • Dagli, M., & Sarıkahya, N. (2018). Elucidation and characterization of glycosidic compounds from Cephalaria tuteliana (dipsacaceae). (master of science thesis), Ege University, Bornova-IZMIR.
  • Dagli, M., Sarikahya, N. B., Nalbantsoy, A., & Kirmizigul, S. (2019). Comparative Phytochemical Screening and Cytotoxic Efficacy of Endemic Cephalaria tuteliana. Natural Product Communications, 14(10), 1934578X19862647.
  • Durmaz, L., Kiziltas, H., Guven, L., Karagecili, H., Alwasel, S., & Gulcin, İ. (2022). Antioxidant, Antidiabetic, Anticholinergic, and Antiglaucoma Effects of Magnofluorine. Molecules, 27(18), 5902.
  • Ellman, G. L., Courtney, K. D., Andres, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2), 88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  • Erdogan, M. K., Gundogdu, R., Yapar, Y., Gecibesler, I. H., Kirici, M., Behcet, L., . . . Taslimi, P. (2023). In vitro anticancer, antioxidant and enzyme inhibitory potentials of endemic Cephalaria elazigensis var. purpurea with in silico studies. Journal of Biomolecular Structure and Dynamics, 1-13. doi:10.1080/07391102.2022.2163700
  • Eruygur, N., Koçyiğit, U. M., Taslimi, P., Ataş, M., Tekin, M., & Gülçin, İ. (2019). Screening the in vitro antioxidant, antimicrobial, anticholinesterase, antidiabetic activities of endemic Achillea cucullata (Asteraceae) ethanol extract. South African Journal of Botany, 120, 141-145. doi:https://doi.org/10.1016/j.sajb.2018.04.001
  • Fogliano, V., Verde, V., Randazzo, G., & Ritieni, A. (1999). Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. Journal of agricultural and food chemistry, 47(3), 1035-1040.
  • Göçer, H., & Gülçin, İ. (2011). Caffeic acid phenethyl ester (CAPE): correlation of structure and antioxidant properties. International Journal of Food Sciences and Nutrition, 62(8), 821-825. doi:10.3109/09637486.2011.585963
  • Göktürk, R. S., & Sümbül, H. (2014). A taxonomic revision of the genus Cephalaria (Caprifoliaceae) in Turkey. Turkish Journal of Botany, 38, 927-968. doi:10.3906/bot-1310-6
  • Gulcin, I. (2009). Antioxidant activity of L-adrenaline: a structure-activity insight. chemicobiological interactions, 179(2), 71-80.
  • Gulcin, İ. (2020). Antioxidants and antioxidant methods: an updated overview. Archives of Toxicology, 94(3), 651-715. doi:10.1007/s00204-020-02689-3
  • Gulçin, İ., Taslimi, P., Aygün, A., Sadeghian, N., Bastem, E., Kufrevioglu, O. I., . . . Şen, F. (2018). Antidiabetic and antiparasitic potentials: Inhibition effects of some natural antioxidant compounds on α-glycosidase, α-amylase and human glutathione S-transferase enzymes. International Journal of Biological Macromolecules, 119, 741-746. https://doi.org/10.1016/j.ijbiomac.2018.08.001
  • Guven, L., Ozgen, U., Secen, H., Sener, S. Ö., Badem, M., Celik, G., & Yayli, N. (2021). Phytochemical studies on the seeds, pseudofruits, and roots of Rosa pimpinellifolia. Journal of Research in Pharmacy, 25(2), 153-163.
  • Gülçin, I. (2008). Measurement of antioxidant ability of melatonin and serotonin by the DMPD and CUPRAC methods as trolox equivalent. Journal of Enzyme Inhibition and Medicinal Chemistry, 23(6), 871-876. https://doi.org/10.1080/14756360701626223
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There are 52 citations in total.

Details

Primary Language Turkish
Subjects Biochemistry and Cell Biology (Other)
Journal Section Gıda Mühendisliği / Food Engineering
Authors

Leyla Güven 0000-0002-3189-6415

Early Pub Date November 30, 2023
Publication Date December 1, 2023
Submission Date August 3, 2023
Acceptance Date September 20, 2023
Published in Issue Year 2023 Volume: 13 Issue: 4

Cite

APA Güven, L. (2023). Cephalaria tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi ve UPLC-MS/MS Analizi ile Kimyasal Karakterizasyonun Belirlenmesi. Journal of the Institute of Science and Technology, 13(4), 2655-2672. https://doi.org/10.21597/jist.1337439
AMA Güven L. Cephalaria tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi ve UPLC-MS/MS Analizi ile Kimyasal Karakterizasyonun Belirlenmesi. J. Inst. Sci. and Tech. December 2023;13(4):2655-2672. doi:10.21597/jist.1337439
Chicago Güven, Leyla. “Cephalaria Tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi Ve UPLC-MS/MS Analizi Ile Kimyasal Karakterizasyonun Belirlenmesi”. Journal of the Institute of Science and Technology 13, no. 4 (December 2023): 2655-72. https://doi.org/10.21597/jist.1337439.
EndNote Güven L (December 1, 2023) Cephalaria tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi ve UPLC-MS/MS Analizi ile Kimyasal Karakterizasyonun Belirlenmesi. Journal of the Institute of Science and Technology 13 4 2655–2672.
IEEE L. Güven, “Cephalaria tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi ve UPLC-MS/MS Analizi ile Kimyasal Karakterizasyonun Belirlenmesi”, J. Inst. Sci. and Tech., vol. 13, no. 4, pp. 2655–2672, 2023, doi: 10.21597/jist.1337439.
ISNAD Güven, Leyla. “Cephalaria Tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi Ve UPLC-MS/MS Analizi Ile Kimyasal Karakterizasyonun Belirlenmesi”. Journal of the Institute of Science and Technology 13/4 (December 2023), 2655-2672. https://doi.org/10.21597/jist.1337439.
JAMA Güven L. Cephalaria tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi ve UPLC-MS/MS Analizi ile Kimyasal Karakterizasyonun Belirlenmesi. J. Inst. Sci. and Tech. 2023;13:2655–2672.
MLA Güven, Leyla. “Cephalaria Tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi Ve UPLC-MS/MS Analizi Ile Kimyasal Karakterizasyonun Belirlenmesi”. Journal of the Institute of Science and Technology, vol. 13, no. 4, 2023, pp. 2655-72, doi:10.21597/jist.1337439.
Vancouver Güven L. Cephalaria tchihatchewii Boiss. Ekstrelerinin Antioksidan Aktivitesi, Metabolik Enzimler Üzerine Etkisi ve UPLC-MS/MS Analizi ile Kimyasal Karakterizasyonun Belirlenmesi. J. Inst. Sci. and Tech. 2023;13(4):2655-72.