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Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney

Year 2023, Volume: 13 Issue: 2, 1101 - 1109, 01.06.2023

Ayşe Yılmaz Veysel Çomaklı

https://doi.org/10.21597/jist.1195089
Cited By: 1

Abstract

Industrial and agricultural processes have brought about the pollution of aquatic systems, and this situation in the water had adverse effects on human health. In order to reduce these negative effects, all mammalian and aquatic species have a defense system. One of these antioxidant systems is the glutathione S-transferase enzyme group, which has detoxification activity against ROS produced by organic pollutants and heavy metals. In this study, the effects of some pesticides and heavy metal ions on the GST enzyme activity purified kidney tissue of the Lake Van fish (C. Tarichii) was analyzed. Firstly, the purification process was carried out as homogenate preparation and glutathione-agarose affinity technique. SDS-PAGE was carried out to check the enzyme purity. The enzyme was obtained in 89% yield (335-fold) with 27.8-specific activity , and kinetic constants was determined for the enzyme; Km for GSH substrate: 0.429 mM Vmax for GSH substrate: 0.207 EU/mL-1; Km for CDNB substrate: 0.276 mM, Vmax for CDNB substrate: 0.161EU/mL-1. Effects of Met-Hg, Fe3+ Cr3+ , Cu2+, Mg2+, Zn2+, atrazine, methomyl ,and dinicanozole were studied on the activity of the purified enzyme and the IC50 values were determined as 34.5 mM, 10 μM, 1.5 μM, 2.4 μM, 63 μM, 0.15 mM, 9.8 μM, 2.67 μM , and 1.3 μM, respectively.

Keywords

Glutathione S-transferase C. tarichii Inhibition some metal pesticide

References

  • Akkemik E, Taser P, Bayindir A, Budak H , Ciftci M , 2012. Purification and characterization of glutathione S-transferase from turkey liver and inhibition effects of some metal ions on enzyme activity. Environmental Toxicology and Pharmacology, 34(3): p. 888-894.
  • Aksoy M, Ozaslan, MS , Kufrevioglu OI, 2016. Purification of glutathione S-transferase from Van Lake fish (Chalcalburnus tarichii Pallas) muscle and investigation of some metal ions effect on enzyme activity. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(4): p. 546-550.
  • Aksoy M., Kufrevioglu OI, 2018. Inhibition of human erythrocyte glutathione S-transferase by some flavonoid derivatives. Toxin Reviews, 37(3): p. 251-257.
  • Alena Tierbach, Ksenia J. Groh, Rene Schonenberger, Kristin Schirmer ,Marc JF,2018. Suter Glutathione S-Transferase Protein Expression in Different Life Stages of Zebrafish (Danio rerio). Toxıcologıcal Sciences, 162(2), 702–712.
  • Bradford M, 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: p. 248-254.
  • Comakli V, Kuzu M, Demirdag R ,2015. Characterization and purification of glutathione S-transferase from the liver and gill tissues of Ağrı Balık Lake trout Salmo trutta labrax and the effects of heavy metal ions on its activity. Journal of Aquatic Animal Health, 27(3): p. 145-151
  • Comakli V, Ciftci M ,Kufrevioglu OI, 2013. Effects of some metal ions on rainbow trout erythrocytes glutathione S-transferase enzyme: an in vitro study. Journal of Enzyme Inhibition and Medicinal Chemistry, 2013. 28(6): p. 1261-1266.
  • Cui J, Li LG, Jiang P, Liu RD, Yang X, Liu LN, Liu P, Zhang SB, Wang ZQ, 2015. Biochemical and functional characterization of the glutathione S-transferase from Trichinella spiralis. Parasitology Research, 114(5): p. 2007-2013.
  • Donham RT, Morin D, Jewell WT, 2005. Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha). Aquat Toxicology,73:221–9.
  • Donham RT, Morin D, Jewell WT, Lame M, Segall H , Tjeerdema RS, 2005. Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha). Aquatic Toxicology, 73(3): p. 221-229.
  • Ekinci D, Beydemir S, Kufrevioglu OI, 2007. In vitro inhibitory effects of some heavy metals on human erythrocyte carbonic anhydrases. Journal of Enzyme Inhibition and Medicinal Chemistry, 22(6): p. 745-750.
  • Ekinci D, Beydemir S, 2010. Purification of PON1 from human serum and assessment of enzyme kinetics against metal toxicity. Biological Trace Element Research, 135(1):p. 112-120.
  • Erat M, Sakiroglu H, 2013. The effect of some antineoplastic agents on glutathione S-transferase from human erythrocytes. Journal of Enzyme Inhibition and Medicinal Chemistry, 28(4): p. 711-716.
  • Glisic, B, Mihaljevic I, Popovic M, Zaja R, Loncar J, Fent K, Kovacevic R, Smital T, 2014. Characterization of Glutathione-S-transferases in zebrafish (Danio rerio), Aquatic Toxicology, 158(1): p. 50-62.
  • Hayes JD, Flanagan JU, Jowsey IR, 2005. Glutathione transferases. Annu Rev Pharmacol Toxicol 45: 51–88.
  • Higgins LG, Hayes JD, 2011. Mechanisms of induction of cytosolic and microsomal glutathione transferase (GST) genes by xenobiotics and pro-inflammatory agents. Drug Metab Rev 43: 92– 137.
  • Huang Q, Liang L, Wei T, 2008. Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Comp Biochem Phys C,147:96–100.
  • Huang Q, Liang L, Wei T, Zhang D , Zeng, Q-Y ,2008. Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 147(1): p. 96-100.
  • Isik M, Demir Y, Kirici M, Demir R, Simsek F, Beydemir S. 2015. Changes in the anti-oxidant system in adult epilepsy patients receiving anti-epileptic drugs. Archives of Physiology and Biochemistry, 121(3): p.97-102.
  • Kuzu M, Aslan A, Ahmed I, Comakli V, Demirdag R , Uzun N, 2016. Purification of glucose-6-phosphate dehydrogenase and glutathione reductase enzymes from the gill tissue of Lake Van fish and analyzing the effects of some chalcone derivatives on enzyme activities. Fish Physiology and Biochemistry, 42(2): p. 483-491.
  • Kuzu M, Ciftci M, 2015. Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. Turk J Chem,39: 149-158.
  • Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259): p. 680.
  • Mozhdeganloo Z, Moghadam Jafari A, Koohi M,2015. Heidarpour Methylmercury-Induced Oxidative Stress in Rainbow Trout (Oncorhynchus mykiss) Liver: Ameliorating Effect of Vitamin C. Biol Trace Elem Res ,165:103–109.
  • Noble E, Barre H ,Dierickx P, 1998. Effect of diet and β-naphthoflavone on hepatic and renal glutathione S-transferase isoenzymes in carp (Cyprinus carpio). Fish Physiology and Biochemistry, 18(2): p. 203-212.
  • Ozaslan MS, Demir Y, Aksoy M, Kufrevioglu OI , Beydemir S,2018. Inhibition effects of pesticides on glutathione‐S‐transferase enzyme activity of Van Lake fish liver. Journal of Biochemical and Molecular Toxicology, 32(9): p. e22196.
  • Ozaslan MS, Demir Y, Kufrevioglu I, Ciftci M, 2017.Some metals inhibit the Glutathione S-transferase from Van Lake fish gills. J.Biochem. Moloxicol,00:e21967.
  • Pabst MJ, Habig WH ,Jakoby WB ,1974. Glutathione S-transferase aa novel kinetic mechanism in which the major reaction pathway depends on substrate concentration. Journal of Biological Chemistry, 249(22): p. 7140-7148.
  • Pérez-López M, Novoa-Valinas MC, Melgar-Riol MJ, 2002. Glutathione S-transferase cytosolic isoforms as biomarkers of polychlorinated biphenyl (Arochlor-1254) experimental contamination in rainbow trout. Toxicology Letters, 136(2): p. 97-106.
  • Pinto E, Sigaud-Kutner TCS, Leitao MAS, Okamoto OK, Morse D, Colepicolo P,2003.Heavymetal-induced oxidative stress in algae”.J Phycol 39:1008–1018.
  • Sau A, Pellizzari Tregno F, Valentino F, Federici G, Caccuri AM, 2010. Glutathione transferases and development of new principles to overcome drug resistance. Arch Biochem Biophys 500: 116– 122.
  • Schwarzenbach RP, Escher BI, Fenner K, Hofstetter TB, Johnson CA, Von Gunten U ,Wehrli B, 2006. The challenge of micropollutants in aquatic systems. Science, 313(5790): p. 1072-1077.
  • Sen A, Kirikbakan A ,2004. Biochemical characterization and distribution of glutathione S-transferases in leaping mullet (Liza saliens). Biochemistry (Moscow), 69(9): p. 993-1000.
  • Soyut H, Beydemir S, 2008. Purification and some kinetic properties of carbonic anhydrase from rainbow trout (Oncorhynchus mykiss) liver and metal inhibition. Protein and Peptide Letters, 15(5): p. 528-535.
  • Topal, A., Atamanalp, M., Oruç, E., Beydemir, Ş., Işık, A., & Demir, Y. (2014). In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout. Archives of Industrial Hygiene and Toxicology, 65(4).
  • Tu CPD, Akgul B, 2005. Drosophila glutathione S‐transferases. Methods in Enzymology, 401: p. 204-226.
  • Turkan F, Huyut Z, Demir Y, Ertas F, Beydemir S, 2019. The effects of some cephalosporins on acetylcholinesterase and glutathione S-transferase: an invivo and invitro study, Archives of Physiology and Biochemistry, 125:3, 235-243.
  • Zhang X, XieP LiD,TangR LeiH, Zhao Y,2009.Time-dependent oxidative stress responses of crucian carp (Carassius auratus) to intraperitoneal injection of extracted microcystins. Bull Environ Contam Toxicol 82: p. 574–578.

Year 2023, Volume: 13 Issue: 2, 1101 - 1109, 01.06.2023

Ayşe Yılmaz Veysel Çomaklı

https://doi.org/10.21597/jist.1195089
Cited By: 1

Abstract

References

  • Akkemik E, Taser P, Bayindir A, Budak H , Ciftci M , 2012. Purification and characterization of glutathione S-transferase from turkey liver and inhibition effects of some metal ions on enzyme activity. Environmental Toxicology and Pharmacology, 34(3): p. 888-894.
  • Aksoy M, Ozaslan, MS , Kufrevioglu OI, 2016. Purification of glutathione S-transferase from Van Lake fish (Chalcalburnus tarichii Pallas) muscle and investigation of some metal ions effect on enzyme activity. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(4): p. 546-550.
  • Aksoy M., Kufrevioglu OI, 2018. Inhibition of human erythrocyte glutathione S-transferase by some flavonoid derivatives. Toxin Reviews, 37(3): p. 251-257.
  • Alena Tierbach, Ksenia J. Groh, Rene Schonenberger, Kristin Schirmer ,Marc JF,2018. Suter Glutathione S-Transferase Protein Expression in Different Life Stages of Zebrafish (Danio rerio). Toxıcologıcal Sciences, 162(2), 702–712.
  • Bradford M, 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: p. 248-254.
  • Comakli V, Kuzu M, Demirdag R ,2015. Characterization and purification of glutathione S-transferase from the liver and gill tissues of Ağrı Balık Lake trout Salmo trutta labrax and the effects of heavy metal ions on its activity. Journal of Aquatic Animal Health, 27(3): p. 145-151
  • Comakli V, Ciftci M ,Kufrevioglu OI, 2013. Effects of some metal ions on rainbow trout erythrocytes glutathione S-transferase enzyme: an in vitro study. Journal of Enzyme Inhibition and Medicinal Chemistry, 2013. 28(6): p. 1261-1266.
  • Cui J, Li LG, Jiang P, Liu RD, Yang X, Liu LN, Liu P, Zhang SB, Wang ZQ, 2015. Biochemical and functional characterization of the glutathione S-transferase from Trichinella spiralis. Parasitology Research, 114(5): p. 2007-2013.
  • Donham RT, Morin D, Jewell WT, 2005. Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha). Aquat Toxicology,73:221–9.
  • Donham RT, Morin D, Jewell WT, Lame M, Segall H , Tjeerdema RS, 2005. Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha). Aquatic Toxicology, 73(3): p. 221-229.
  • Ekinci D, Beydemir S, Kufrevioglu OI, 2007. In vitro inhibitory effects of some heavy metals on human erythrocyte carbonic anhydrases. Journal of Enzyme Inhibition and Medicinal Chemistry, 22(6): p. 745-750.
  • Ekinci D, Beydemir S, 2010. Purification of PON1 from human serum and assessment of enzyme kinetics against metal toxicity. Biological Trace Element Research, 135(1):p. 112-120.
  • Erat M, Sakiroglu H, 2013. The effect of some antineoplastic agents on glutathione S-transferase from human erythrocytes. Journal of Enzyme Inhibition and Medicinal Chemistry, 28(4): p. 711-716.
  • Glisic, B, Mihaljevic I, Popovic M, Zaja R, Loncar J, Fent K, Kovacevic R, Smital T, 2014. Characterization of Glutathione-S-transferases in zebrafish (Danio rerio), Aquatic Toxicology, 158(1): p. 50-62.
  • Hayes JD, Flanagan JU, Jowsey IR, 2005. Glutathione transferases. Annu Rev Pharmacol Toxicol 45: 51–88.
  • Higgins LG, Hayes JD, 2011. Mechanisms of induction of cytosolic and microsomal glutathione transferase (GST) genes by xenobiotics and pro-inflammatory agents. Drug Metab Rev 43: 92– 137.
  • Huang Q, Liang L, Wei T, 2008. Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Comp Biochem Phys C,147:96–100.
  • Huang Q, Liang L, Wei T, Zhang D , Zeng, Q-Y ,2008. Purification and partial characterization of glutathione transferase from the teleost Monopterus albus. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 147(1): p. 96-100.
  • Isik M, Demir Y, Kirici M, Demir R, Simsek F, Beydemir S. 2015. Changes in the anti-oxidant system in adult epilepsy patients receiving anti-epileptic drugs. Archives of Physiology and Biochemistry, 121(3): p.97-102.
  • Kuzu M, Aslan A, Ahmed I, Comakli V, Demirdag R , Uzun N, 2016. Purification of glucose-6-phosphate dehydrogenase and glutathione reductase enzymes from the gill tissue of Lake Van fish and analyzing the effects of some chalcone derivatives on enzyme activities. Fish Physiology and Biochemistry, 42(2): p. 483-491.
  • Kuzu M, Ciftci M, 2015. Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. Turk J Chem,39: 149-158.
  • Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259): p. 680.
  • Mozhdeganloo Z, Moghadam Jafari A, Koohi M,2015. Heidarpour Methylmercury-Induced Oxidative Stress in Rainbow Trout (Oncorhynchus mykiss) Liver: Ameliorating Effect of Vitamin C. Biol Trace Elem Res ,165:103–109.
  • Noble E, Barre H ,Dierickx P, 1998. Effect of diet and β-naphthoflavone on hepatic and renal glutathione S-transferase isoenzymes in carp (Cyprinus carpio). Fish Physiology and Biochemistry, 18(2): p. 203-212.
  • Ozaslan MS, Demir Y, Aksoy M, Kufrevioglu OI , Beydemir S,2018. Inhibition effects of pesticides on glutathione‐S‐transferase enzyme activity of Van Lake fish liver. Journal of Biochemical and Molecular Toxicology, 32(9): p. e22196.
  • Ozaslan MS, Demir Y, Kufrevioglu I, Ciftci M, 2017.Some metals inhibit the Glutathione S-transferase from Van Lake fish gills. J.Biochem. Moloxicol,00:e21967.
  • Pabst MJ, Habig WH ,Jakoby WB ,1974. Glutathione S-transferase aa novel kinetic mechanism in which the major reaction pathway depends on substrate concentration. Journal of Biological Chemistry, 249(22): p. 7140-7148.
  • Pérez-López M, Novoa-Valinas MC, Melgar-Riol MJ, 2002. Glutathione S-transferase cytosolic isoforms as biomarkers of polychlorinated biphenyl (Arochlor-1254) experimental contamination in rainbow trout. Toxicology Letters, 136(2): p. 97-106.
  • Pinto E, Sigaud-Kutner TCS, Leitao MAS, Okamoto OK, Morse D, Colepicolo P,2003.Heavymetal-induced oxidative stress in algae”.J Phycol 39:1008–1018.
  • Sau A, Pellizzari Tregno F, Valentino F, Federici G, Caccuri AM, 2010. Glutathione transferases and development of new principles to overcome drug resistance. Arch Biochem Biophys 500: 116– 122.
  • Schwarzenbach RP, Escher BI, Fenner K, Hofstetter TB, Johnson CA, Von Gunten U ,Wehrli B, 2006. The challenge of micropollutants in aquatic systems. Science, 313(5790): p. 1072-1077.
  • Sen A, Kirikbakan A ,2004. Biochemical characterization and distribution of glutathione S-transferases in leaping mullet (Liza saliens). Biochemistry (Moscow), 69(9): p. 993-1000.
  • Soyut H, Beydemir S, 2008. Purification and some kinetic properties of carbonic anhydrase from rainbow trout (Oncorhynchus mykiss) liver and metal inhibition. Protein and Peptide Letters, 15(5): p. 528-535.
  • Topal, A., Atamanalp, M., Oruç, E., Beydemir, Ş., Işık, A., & Demir, Y. (2014). In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout. Archives of Industrial Hygiene and Toxicology, 65(4).
  • Tu CPD, Akgul B, 2005. Drosophila glutathione S‐transferases. Methods in Enzymology, 401: p. 204-226.
  • Turkan F, Huyut Z, Demir Y, Ertas F, Beydemir S, 2019. The effects of some cephalosporins on acetylcholinesterase and glutathione S-transferase: an invivo and invitro study, Archives of Physiology and Biochemistry, 125:3, 235-243.
  • Zhang X, XieP LiD,TangR LeiH, Zhao Y,2009.Time-dependent oxidative stress responses of crucian carp (Carassius auratus) to intraperitoneal injection of extracted microcystins. Bull Environ Contam Toxicol 82: p. 574–578.
There are 37 citations in total.

Details

Primary Language English
Subjects Chemical Engineering
Journal Section Kimya / Chemistry
Authors

Ayşe Yılmaz This is me 0000-0002-1548-9318

Veysel Çomaklı 0000-0003-2109-6702

Early Pub Date May 27, 2023
Publication Date June 1, 2023
Submission Date November 17, 2022
Acceptance Date January 27, 2023
Published in Issue Year 2023 Volume: 13 Issue: 2

Cite

APA Yılmaz, A., & Çomaklı, V. (2023). Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney. Journal of the Institute of Science and Technology, 13(2), 1101-1109. https://doi.org/10.21597/jist.1195089
AMA Yılmaz A, Çomaklı V. Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney. J. Inst. Sci. and Tech. June 2023;13(2):1101-1109. doi:10.21597/jist.1195089
Chicago Yılmaz, Ayşe, and Veysel Çomaklı. “Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney”. Journal of the Institute of Science and Technology 13, no. 2 (June 2023): 1101-9. https://doi.org/10.21597/jist.1195089.
EndNote Yılmaz A, Çomaklı V (June 1, 2023) Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney. Journal of the Institute of Science and Technology 13 2 1101–1109.
IEEE A. Yılmaz and V. Çomaklı, “Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney”, J. Inst. Sci. and Tech., vol. 13, no. 2, pp. 1101–1109, 2023, doi: 10.21597/jist.1195089.
ISNAD Yılmaz, Ayşe - Çomaklı, Veysel. “Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney”. Journal of the Institute of Science and Technology 13/2 (June 2023), 1101-1109. https://doi.org/10.21597/jist.1195089.
JAMA Yılmaz A, Çomaklı V. Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney. J. Inst. Sci. and Tech. 2023;13:1101–1109.
MLA Yılmaz, Ayşe and Veysel Çomaklı. “Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney”. Journal of the Institute of Science and Technology, vol. 13, no. 2, 2023, pp. 1101-9, doi:10.21597/jist.1195089.
Vancouver Yılmaz A, Çomaklı V. Investigation of Effects of Some Metal Ions and Some Pesticides on Glutathione S-Transferase (GST) Enzyme Purified from Van Lake Fish (Chalcalburnus Tarichi) Kidney. J. Inst. Sci. and Tech. 2023;13(2):1101-9.

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