Research Article
Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi
Abstract
Corylus avellana L. çoğunlukla Giresun’da yetişen dünyanın en kaliteli fındık türlerinden biridir. Bu çalışma, Giresun Tombul fındık yağının (0.5, 5 ve 50 mg L-1) antikanser ve antianjiyojenik aktivitelerini değerlendirmek amacıyla gerçekleştirilmiştir. Sitotoksisite insan serviks, kolon ve meme kanseri hücreleri üzerinde MTT ve laktat dehidrojenaz salım (LDH) analizleri ile belirlenmiştir. Hücrelerin oksidatif durumu, toplam oksidatif stres (TOS) ve toplam antioksidan kapasite (TAK) yöntemleriyle ortaya konulmuştur. Anti-anjiyojenik aktiviteyi değerlendirmek için civciv koryoallantoik membran (HET-CAM) deneyi kullanılmıştır. Sonuçlarımız, C. avellana yağının özellikle serviks kanseri hücrelerinde daha fazla olmak üzere, serviks ve kolon kanseri hücrelerinin (sırasıyla IC 50 = 6.5 ± 0.35 mg L-1 ve IC 50 = 26.2 ± 3.15 mg L-1) canlılığını önemli ölçüde inhibe ettiğini göstermiştir. Bununla birlikte, fındık yağ örneği, LDH salınımında önemli bir inhibisyona yol açarak sitotoksisite ile doğrusal bir korelasyon sergilemiştir. C. avellana yağ örneği konsantrasyona bağlı bir şekilde serviks ve kolon hücrelerinin TAK seviyelerinde önemli bir artışa neden olurken, TOS seviyelerinde istatistiksel olarak önemli azalmaya yol açmıştır. Aynı zamanda, C. avellana yağ örneği yüksek konsantrasyonlarda anti-anjiyojenik etkinlik göstermiştir. Sonuçlar, Giresun fındığının umut verici bir antikanser ajan olduğunu ve bununla birlikte terapötik etkinliğini ortaya koymak için daha ileri çalışmalara ihtiyaç olduğunu göstermiştir.
Supporting Institution
Giresun Üniversitesi
Project Number
FEN-BAP-A-150219-28
References
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- Benetou V, Trichopoulou A, Orfanos P, Naska A, Lagiou P, Boffetta P, Trichopoulos D, 2008. Greek EPIC cohort. Conformity to traditional Mediterranean diet and cancer incidence: the Greek EPIC cohort. British Journal of Cancer, 99(1): 191-195.
- Ciardiello F, Caputo R, Bianco R, Damiano V, Pomatico G, De Placido S, Bianco AR, Tortora G, 2000. Antitumor effect and potentiation of cytotoxic drugs activity in human cancer cells by ZD-1839 (Iressa), an epidermal growth factor receptor-selective tyrosine kinase inhibitor. Clinical Cancer Research, 6(5): 2053-63.
- Ciarmiello LF, Mazzeo MF, Minasi P, Peluso A, de Luca A, Piccirillo P, Siciliano RA, Carbone V, 2014. Analysis of Different European Hazelnut (Corylus avellana L.) Cultivars: Authentication, Phenotypic Features, and Phenolic Profiles. Journal of Agricultural and Food Chemistry, 62(26): 6236-6246.
- Colditz GA, Wolin KY, Gehlert S, 2012. Applying what we know to accelerate cancer prevention. Science Translational Medicine, 4(127): 127-1244.
- Denekamp J, 1993. Review article: angiogenesis, neovascular proliferation and vascular pathophysiology as targets for cancer therapy. British Journal of Radiology, 66(783): 181-96.
- Eming SA, Brachvogel B, Odorisio T. Koch M, 2007. Regulation of angiogenesis: Wound healing as a model. Progress in Histochemistry and Cytochemistry, 42(3): 115-170.
- Esposito T, Sansone F, Franceschelli S, Del Gaudio P, Picerno P, Aquino RP, Mencherini T, 2017. Hazelnut (Corylus avellana L.) Shells Extract: Phenolic Composition, Antioxidant Effect and Cytotoxic Activity on Human Cancer Cell Lines. International Journal of Molecular Sciences, 18(2): 392.
- Falasca M. Casari I, 2012. Cancer chemoprevention by nuts: evidence and promises. Frontiers in bioscience (Scholar edition), 4: 109-120.
- Falasca, M., Casari, I. & Maffucci, T, (2014). Cancer chemoprevention with nuts. Journal of the National Cancer Institute, 106(9): dju238.
- Fang L, Ren D, Wang Z, Liu C, Wang J, Min W, 2019. Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells. Journal of Food Biochemistry, 43(3): e12722.
- Fischer S, Glei M, 2013. Health-Potential of Nuts. Ernaehrungs Umschau international, 60, 206-215.
- Folkman J. 1995. Angiogenesis in cancer, vascular, rheumatoid and other disease, Nature medicine, 1(1): 27-31.
- Green DR, Reed JC, 1998. Mitochondria and apoptosis. Science, 281: 1309-1312.
- Güner A, Karabay Yavaşoğlu NÜ, 2018. Evaluation of antioxidant, antimicrobial and antimutagenic activity with irritation effects of Ceramium rubrum (Red Algae) extract. International Journal of Secondary Metabolite, 5(4): 279-287.
- Güner A, Polatlı E, Akkan T, Bektaş H, Albay C, 2019a. Anticancer and antiangiogenesis activities of novel synthesized 2-substituted benzimidazoles molecules. Turkish Journal of Chemistry, 43(5): 1270–1289.
- Güner A, Nalbantsoy A, Sukatar A., Karabay Yavaşoğlu NU, (2019b). Apoptosis-inducing activities of Halopteris scoparia L. Sauvageau (Brown algae) on cancer cells and its biosafety and antioxidant properties. Cytotechnology, 71(3): 687-704.
- Güner A, Kızılşahin S, Nalbantsoy A, Karabay Yavaşoğlu NU, 2020. Apoptosis-inducing activity of safflower (Carthamus tinctorius L.) seed oil in lung, colorectal and cervix cancer cells. Biologia 75: 1465-1471.
- Güner A, 2020. Toxic and irritant effects induced by zearalenone: prevention by taurine. Toxin Reviews, 1-10. Doi: 10.1080/15569543.2020.1777432
- Güner A, İlhan S, 2020. Cytotoxic, genotoxic, oxidative, and irritant effects of zinc pyrithione in vitro. Toxicological & Environmental Chemistry, 102(10): 607-623
- Köksal AI, 2002. Türk Fındık Çeşitleri. A.Ü. Ziraat Fakültesi Bahçe Bitkileri Bölümü, Ankara. ISBN 975-92886-0-5.
- Köksal AI, Artik N, Simsek A, Gunes N, 2006. Nutrient Composition of Hazelnut (Corylus avellana L.) Varieties Cultivated in Turkey. Food Chemistry, 99(3): 509-515.
- Kim, Y.W. & Byzova, T.V. (2014). Oxidative stress in angiogenesis and vascular disease, Blood, 123: 625-631.
- Koh YW, Lee SJ, Park SY, 2017. Prognostic significance of lactate dehydrogenase B according to histologic type of non‐small‐cell lung cancer and its association with serum lactate dehydrogenase. Pathology‐Research and Practice, 213: 1134-1138.
- La Vecchia C, 2009. Association between Mediterranean dietary patterns and cancer risk. Nutrition Reviews, 67(1): 126-129.
- Lui RHJ, 2003. Health benefi ts of fruits and vegetables are from additive and synergestic combination of phytochemicals. The American Journal of Clinical Nutrition,78: 517-520.
- Lui RHJ, 2004. Potential synergy of phytochemicals in cancer prevention: mechanism of action. Journal of Nutrition, 134: 3479-3485.
- Liou GY, Storz P, 2010. Reactive oxygen species in cancer. Free Radical Research, 44: 479-496.
- Madesis P, Ganopoulos I, Bosmali I, Tsaftaris A, Barcode H, 2013. Resolution Melting analysis for forensic uses in nuts: A case study on allergenic hazelnuts (Corylus avellana). Food Research International, 50, 351-360.
- Muslim N, Nassar Z, Aisha A, Shafaei A, Idris N, Majid A, Ismail Z, 2012. Antiangiogenesis and antioxidant activity of ethanol extracts of Pithecellobium jiringa. BMC Complementary Medicine and Therapies, 12: 210.
- Nishida N, Yano H, Nishida T, Kamura T, Kojiro M, 2006. Angiogenesis in cancer. Vascular Health and Risk Management, 2: 213-219.
- Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB, 2010. Oxidative stress, inflammation, and cancer: how are they linked? Free radical biology & medicine, 49(11): 1603-1616.
- Riboli E, 2014. The role of metabolic carcinogenesis in cancer causation and prevention: evidence from the European Prospective Investigation into Cancer and Nutrition. Cancer Treatment and Research, 159: 3-20.
- Schmitzer V, Slatnar A, Veberic R, Stampar F, Solar A, 2011. Roasting affects phenolic composition and antioxidative activity of hazelnuts (Corylus avellana L.). Journal of Food Science, 76: 14-19.
- Valko M, Rhodes C, Moncol J, Izakovic MM, Mazur M, 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-biological interactions, 160(1): 1-40.
- Visconti R, Grieco D, 2009. New insights on oxidative stress in cancer. Current opinion in drug discovery & development, 12(2): 240-245.
Antiangiogenic and Cytotoxic Activity of Giresun Tombul Hazelnut (Corylus avellana L.) Oil on Cervix, Breast, and Colon Cancer Cells
Abstract
Corylus avellana L. is one of the best quality hazelnut species grown mostly in Giresun province. This present study was realised to evaluate the anticancer and antiangiogenic activities of Giresun Tombul hazelnut oil (0.5, 5 and 50 mg L-1). Cytotoxicity was determined by MTT and lactate dehydrogenase release (LDH) assays on human cervix, colon, and breast cancer cells. Oxidative status was analyzed by total oxidative stress (TOS) and total antioxidant capacity (TAC) assay. Hen’s egg test chorioallantoic membrane (HET-CAM) assay was used to evaluate the anti-angiogenic activity. Our results showed that C. avellana oil had significant anti-proliferative activity against both cervix and colon cancer cells (6.5 ± 0.35 mg L-1 and 26.26 ± 3.15 mg L-1, respectively), with more inhibiting the cervix cancers and caused significant inhibition in LDH release as a linear correlation with MTT results. C. avellana oil significantly increased the TAC status on the cervix and colon cells while causing a significant reduction in TOS levels in a dose-dependent manner. C. avellana oil had anti-angiogenic effectiveness in higher doses. The results indicated that Giresun hazelnut was a promising anticancer agent and however, further studies are needed to reveal its therapeutic effectiveness.
Project Number
FEN-BAP-A-150219-28
References
- ACS, 2018. Cancer Facts and Figures. American Cancer Society, New York, NY, USA.
- Alasalvar C, Shahidi F, Liyana-Pathirana C, Ohshima T, 2003. Turkish Tombul hazelnut (Corylus avellana L.). 1. Composition characteristics. Journal of Agricultural and Food Chemistry, 51(13): 3790-3796.
- Benetou V, Trichopoulou A, Orfanos P, Naska A, Lagiou P, Boffetta P, Trichopoulos D, 2008. Greek EPIC cohort. Conformity to traditional Mediterranean diet and cancer incidence: the Greek EPIC cohort. British Journal of Cancer, 99(1): 191-195.
- Ciardiello F, Caputo R, Bianco R, Damiano V, Pomatico G, De Placido S, Bianco AR, Tortora G, 2000. Antitumor effect and potentiation of cytotoxic drugs activity in human cancer cells by ZD-1839 (Iressa), an epidermal growth factor receptor-selective tyrosine kinase inhibitor. Clinical Cancer Research, 6(5): 2053-63.
- Ciarmiello LF, Mazzeo MF, Minasi P, Peluso A, de Luca A, Piccirillo P, Siciliano RA, Carbone V, 2014. Analysis of Different European Hazelnut (Corylus avellana L.) Cultivars: Authentication, Phenotypic Features, and Phenolic Profiles. Journal of Agricultural and Food Chemistry, 62(26): 6236-6246.
- Colditz GA, Wolin KY, Gehlert S, 2012. Applying what we know to accelerate cancer prevention. Science Translational Medicine, 4(127): 127-1244.
- Denekamp J, 1993. Review article: angiogenesis, neovascular proliferation and vascular pathophysiology as targets for cancer therapy. British Journal of Radiology, 66(783): 181-96.
- Eming SA, Brachvogel B, Odorisio T. Koch M, 2007. Regulation of angiogenesis: Wound healing as a model. Progress in Histochemistry and Cytochemistry, 42(3): 115-170.
- Esposito T, Sansone F, Franceschelli S, Del Gaudio P, Picerno P, Aquino RP, Mencherini T, 2017. Hazelnut (Corylus avellana L.) Shells Extract: Phenolic Composition, Antioxidant Effect and Cytotoxic Activity on Human Cancer Cell Lines. International Journal of Molecular Sciences, 18(2): 392.
- Falasca M. Casari I, 2012. Cancer chemoprevention by nuts: evidence and promises. Frontiers in bioscience (Scholar edition), 4: 109-120.
- Falasca, M., Casari, I. & Maffucci, T, (2014). Cancer chemoprevention with nuts. Journal of the National Cancer Institute, 106(9): dju238.
- Fang L, Ren D, Wang Z, Liu C, Wang J, Min W, 2019. Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells. Journal of Food Biochemistry, 43(3): e12722.
- Fischer S, Glei M, 2013. Health-Potential of Nuts. Ernaehrungs Umschau international, 60, 206-215.
- Folkman J. 1995. Angiogenesis in cancer, vascular, rheumatoid and other disease, Nature medicine, 1(1): 27-31.
- Green DR, Reed JC, 1998. Mitochondria and apoptosis. Science, 281: 1309-1312.
- Güner A, Karabay Yavaşoğlu NÜ, 2018. Evaluation of antioxidant, antimicrobial and antimutagenic activity with irritation effects of Ceramium rubrum (Red Algae) extract. International Journal of Secondary Metabolite, 5(4): 279-287.
- Güner A, Polatlı E, Akkan T, Bektaş H, Albay C, 2019a. Anticancer and antiangiogenesis activities of novel synthesized 2-substituted benzimidazoles molecules. Turkish Journal of Chemistry, 43(5): 1270–1289.
- Güner A, Nalbantsoy A, Sukatar A., Karabay Yavaşoğlu NU, (2019b). Apoptosis-inducing activities of Halopteris scoparia L. Sauvageau (Brown algae) on cancer cells and its biosafety and antioxidant properties. Cytotechnology, 71(3): 687-704.
- Güner A, Kızılşahin S, Nalbantsoy A, Karabay Yavaşoğlu NU, 2020. Apoptosis-inducing activity of safflower (Carthamus tinctorius L.) seed oil in lung, colorectal and cervix cancer cells. Biologia 75: 1465-1471.
- Güner A, 2020. Toxic and irritant effects induced by zearalenone: prevention by taurine. Toxin Reviews, 1-10. Doi: 10.1080/15569543.2020.1777432
- Güner A, İlhan S, 2020. Cytotoxic, genotoxic, oxidative, and irritant effects of zinc pyrithione in vitro. Toxicological & Environmental Chemistry, 102(10): 607-623
- Köksal AI, 2002. Türk Fındık Çeşitleri. A.Ü. Ziraat Fakültesi Bahçe Bitkileri Bölümü, Ankara. ISBN 975-92886-0-5.
- Köksal AI, Artik N, Simsek A, Gunes N, 2006. Nutrient Composition of Hazelnut (Corylus avellana L.) Varieties Cultivated in Turkey. Food Chemistry, 99(3): 509-515.
- Kim, Y.W. & Byzova, T.V. (2014). Oxidative stress in angiogenesis and vascular disease, Blood, 123: 625-631.
- Koh YW, Lee SJ, Park SY, 2017. Prognostic significance of lactate dehydrogenase B according to histologic type of non‐small‐cell lung cancer and its association with serum lactate dehydrogenase. Pathology‐Research and Practice, 213: 1134-1138.
- La Vecchia C, 2009. Association between Mediterranean dietary patterns and cancer risk. Nutrition Reviews, 67(1): 126-129.
- Lui RHJ, 2003. Health benefi ts of fruits and vegetables are from additive and synergestic combination of phytochemicals. The American Journal of Clinical Nutrition,78: 517-520.
- Lui RHJ, 2004. Potential synergy of phytochemicals in cancer prevention: mechanism of action. Journal of Nutrition, 134: 3479-3485.
- Liou GY, Storz P, 2010. Reactive oxygen species in cancer. Free Radical Research, 44: 479-496.
- Madesis P, Ganopoulos I, Bosmali I, Tsaftaris A, Barcode H, 2013. Resolution Melting analysis for forensic uses in nuts: A case study on allergenic hazelnuts (Corylus avellana). Food Research International, 50, 351-360.
- Muslim N, Nassar Z, Aisha A, Shafaei A, Idris N, Majid A, Ismail Z, 2012. Antiangiogenesis and antioxidant activity of ethanol extracts of Pithecellobium jiringa. BMC Complementary Medicine and Therapies, 12: 210.
- Nishida N, Yano H, Nishida T, Kamura T, Kojiro M, 2006. Angiogenesis in cancer. Vascular Health and Risk Management, 2: 213-219.
- Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB, 2010. Oxidative stress, inflammation, and cancer: how are they linked? Free radical biology & medicine, 49(11): 1603-1616.
- Riboli E, 2014. The role of metabolic carcinogenesis in cancer causation and prevention: evidence from the European Prospective Investigation into Cancer and Nutrition. Cancer Treatment and Research, 159: 3-20.
- Schmitzer V, Slatnar A, Veberic R, Stampar F, Solar A, 2011. Roasting affects phenolic composition and antioxidative activity of hazelnuts (Corylus avellana L.). Journal of Food Science, 76: 14-19.
- Valko M, Rhodes C, Moncol J, Izakovic MM, Mazur M, 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-biological interactions, 160(1): 1-40.
- Visconti R, Grieco D, 2009. New insights on oxidative stress in cancer. Current opinion in drug discovery & development, 12(2): 240-245.
There are 37 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Structural Biology |
| Journal Section | Biyoloji / Biology |
| Authors | |
| Project Number | FEN-BAP-A-150219-28 |
| Publication Date | June 1, 2021 |
| Submission Date | October 24, 2020 |
| Acceptance Date | February 26, 2021 |
| Published in Issue | Year 2021 Volume: 11 Issue: 2 |