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Hen’s Egg Test For Micronucleus Induction (HET-MN)

Year 2016, Volume: 6 Issue: 4, 17 - 28, 31.12.2016

Haluk Özparlak Bülent Çelik

Abstract

One of widely used methods in genotoxicity tests is micronucleus technique. Hen’s
Egg Test for Micronucleus Induction (HET-MN) is a member of relatively new of micronucleus tests.
HET-MN is an extremely simple, inexpensive and rapid genotoxicity assay which is positioned between
pure in vitro and in vivo analysis, strictly in line with animal protection regulations and ethical aspects.
Furthermore the high metabolic competency provided by this model enables metabolic activation of
xenobiotics including mutagens and promutagens. In this article, recent publications about the kinds of
micronucleus tests and then the application of HET-MN have been compiled  

Keywords

Chicken embryo erythrocyte genotoxicity micronucleus

References

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  • Aymak C, 2010. Mersin bölgesinde yaşayan Rana ridibunda pallas, 1771 (Ranidae, Ampibia)’nın mikronükleus test yöntemi ile genotoksik etkilerinin ve ağır metal kirliliğinin tespit edilmesi. Mersin Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, Mersin.
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  • Çavaş T, 2004. Endüstriyel atıkların genotoksik etkilerinin mikronükleus ve AgNOR analiz teknikleri kullanılarak insitu ve laboratuvar koşulları altında araştırılması. Mersin Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, Mersin.
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  • Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E, 2003. Human Micronucleus Project. HUMN Project: Detailed description of the scoring criteria for the cytokinesis– block micronucleus assay using isolated human lymphocyte cultures. Mutation Research-Gen Tox En, 534: 65-75.
  • Fenech M, 2000. The in vitro micronucleus technique. Mutation Research-Fund Mol, 435: 81-95.
  • Fenech M, Crott J, Turner J, Brown S, 1999. Necrosis, apoptosis, cytostasis and DNA damage in human lymphocytes measured simultaneously within the cytokinesis-block micronucleus assay: Description of the method and results for hydrogen peroxide. Mutagenesis, 14(6): 605-612.
  • Fenech M, Morley AA, 1985. Measurement of micronuclei in lymphocytes. Mutation Research, 147: 29-36.
  • Giri S, Sharma GD, Giri A, Prasad SB, 2002. Genotoxic effects of malathion in chick in vivo micronucleus assay. Cytologia, 67: 53-59.
  • Gomez-Arroyo S, Díaz-Sánchez Y, Meneses-Pérez MA, VillalobosPietrini R, De León-Rodríguez J, 2000. Cytogenetic biomonitoring in a mexican floriculture worker group exposed to pesticides. Mutation Research, 466: 117-124.
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Tavuk Yumurtası Mikronükleus Testi

Year 2016, Volume: 6 Issue: 4, 17 - 28, 31.12.2016

Haluk Özparlak Bülent Çelik

Abstract

Genotoksisite testleri içinde çok yaygın kullanılan yöntemlerden biri mikronükleus tekniğidir.
Mikronükleus testleri içerisinde ise diğerlerine kıyasla daha yeni bir teknik Tavuk Yumurtası Mikronükleus
Testi (Hen’s Egg Test for Micronucleus Induction, HET-MN)’dir. HET-MN hayvan hakları ve etik
açıdan kesinlikle uygun, in vitro ve in vivo deneyler arasına yerleştirebileceğimiz son derece basit, ucuz
ve hızlı bir genotoksisite testidir. Ayrıca bu modelin sağladığı yüksek metabolik yetkinlik mutajen ve
promutajenleri içeren ksenobiyotiklerin metabolik aktivasyonuna da imkân tanımaktadır. Bu makalede
önce mikronükleus testlerinin çeşitleri, ardından HET-MN’nin uygulamasıyla ilgili güncel yayınlar
derlenmiştir  

Keywords

Eritrosit genotoksisite mikronükleus tavuk embriyosu

References

  • Alakoç C, 2010. Gazaltı kaynağından çıkan gazlara maruz kalan kişilerin periferal kan lenfositlerindeki mitotik indeks, replikasyon indeksi ve mikronükleus parametrelerinin değerlendirilmesi. Bozok Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Yozgat.
  • Al-Sabti K, Metcalfe CD, 1995. Fish micronuclei for assessing genotoxicity in water. Mutation Research, 343: 121-135.
  • Al-Sabti K, 1986. Comparative micronucleated erythrocyte cell induction in three cyprinids by fve carcinogenic-mutagenic chemicals. Cytobios, 47: 147-154.
  • Aydın SA, 2007. Fluniksin megluminin genotoksisitesinin in vitro ve in vivo/in vitro mikronükleus testleriyle değerlendirilmesi. İstanbul Üniversitesi Sağlık Bilimler Enstitüsü, Doktora Tezi, İstanbul.
  • Ayllon F, Garcia-Vazquez E, 2000. Induction of micronuclei and other nuclear abnormalities in European minnow Phoxinus phoxinus and mollie Poecilia latipinna: an assessment of the fsh micronucleus test. Mutation Research, 467: 177-186.
  • Aymak C, 2010. Mersin bölgesinde yaşayan Rana ridibunda pallas, 1771 (Ranidae, Ampibia)’nın mikronükleus test yöntemi ile genotoksik etkilerinin ve ağır metal kirliliğinin tespit edilmesi. Mersin Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, Mersin.
  • Bhunya SP, Jena GB, 1993. Studies on the genotoxicity of monocrotophos, an organophosphate insecticide, in the chick in vivo test system. Mutation Research, 292: 231-239.
  • Bhunya SP, Jena GB, 1996. Clastogenic effects of copper sulphate in chick in vivo test system. Mutation Research, 367 (2): 57- 63.
  • Bhunya SP, Jena GB, 1992. Genotoxic potential of the organochlorine insecticide lindane (γ-BHC): An in vivo study in chicks. Mutation Research, 272: 175-181.
  • Bruns GAP, Ingram VM, 1973. The erythroid cells and haemoglobins of the chick embryo. Philosophical Transactions of the Royal Society, 266: 225-305.
  • Campana MA, Panzeri AM, Moreno VJ, Dulout FN, 2003. Micronuclei induction in Rana catesbeiana tadpoles by the pyrethroid insecticide lambda-chalothrin. Genetics and Molecular Biology, 26: 99- 103.
  • Choy WN, 2001. Genetic Toxicology and Cancer Risk Assessment, Marcel Dekker, New York, 406 p.
  • Cunny H, Hodgson E, 2004. Toxicity Testing, In: Hodgson, E. (ed.), A Textbook of Modern Toxicology, John Wiley & Sons, Inc., USA, p. 353-397.
  • Çakır Ş, 2004. Bazı organik fosforlu insektisitlerin Drosophila melanogaster’in yaşama yüzdesi üzerine etkisi. Gazi Üniversitesi Gazi Eğitim Fakültesi Dergisi, 24(3): 71-80.
  • Çavaş T, Ergene-Gözükara S, 2003. Micronuclei, nuclear lesions and interphase silver-stained nucleolar organizer regions (AgNORs) as cyto-genotoxicity indicators in Oreochromis niloticus exposed to textile mill effluent. Mutation ResearchGen Tox En, 538: 81-91.
  • Çavaş T, 2004. Endüstriyel atıkların genotoksik etkilerinin mikronükleus ve AgNOR analiz teknikleri kullanılarak insitu ve laboratuvar koşulları altında araştırılması. Mersin Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, Mersin.
  • Çavuşoğlu K, Yalçın E, Dönmez S, Kaymaz K, Özdemir G, Özgörür Z, Balcı D, Aslan B, Çakır M. 2008. Vicia faba L. (Fabaceae) kök ucu hücrelerinde fenol tarafından teşvik edilen sitotoksisitenin belirlenmesi. SDÜ Fen Dergisi (E-Dergi) 3(2): 139-148.
  • Demirel S, Zamani A. 2002. MN tekniği ve kullanım alanları. Genel Tıp Dergisi, 12(3): 123-127.
  • Duffaud F, Orsiere T, Villani P, Pelissier AL, Volot F, Favre R, 1997. Comparison between micronucleated lymphocytes rates observed in healthy subject and cancer patients. Mutagenesis, 12: 227-231.
  • Ergene Gözükara S, Çavaş T, Aymak C, 2001. Cypermethrin’in Oreochromis niloticus (L.,1758) üzerindeki genotoksik etkilerin mikronükleus testi ile araştırılması. XI. Ulusal Su Ürünleri Sempozyumu, 2: 765-771.
  • Ergene S, Kaya Ş, Dürgen A, Uçar A, Erkek M, Ergene M, Könen AS, Özbaba O, Önder H, 2012. Mersin rehabilitasyon merkezinde tedavi altında bulunan yaralı deniz kaplumbağalarının hematolojik ve biyokimyasal özelliklerinin incelenmesi, araştırma makalesi. Anadolu Doğa Bilimleri Dergisi 3(2): 34- 40.
  • Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E, 2003. Human Micronucleus Project. HUMN Project: Detailed description of the scoring criteria for the cytokinesis– block micronucleus assay using isolated human lymphocyte cultures. Mutation Research-Gen Tox En, 534: 65-75.
  • Fenech M, 2000. The in vitro micronucleus technique. Mutation Research-Fund Mol, 435: 81-95.
  • Fenech M, Crott J, Turner J, Brown S, 1999. Necrosis, apoptosis, cytostasis and DNA damage in human lymphocytes measured simultaneously within the cytokinesis-block micronucleus assay: Description of the method and results for hydrogen peroxide. Mutagenesis, 14(6): 605-612.
  • Fenech M, Morley AA, 1985. Measurement of micronuclei in lymphocytes. Mutation Research, 147: 29-36.
  • Giri S, Sharma GD, Giri A, Prasad SB, 2002. Genotoxic effects of malathion in chick in vivo micronucleus assay. Cytologia, 67: 53-59.
  • Gomez-Arroyo S, Díaz-Sánchez Y, Meneses-Pérez MA, VillalobosPietrini R, De León-Rodríguez J, 2000. Cytogenetic biomonitoring in a mexican floriculture worker group exposed to pesticides. Mutation Research, 466: 117-124.
  • GPT, 2006. Micronucleus assay. GenPharmTox. http://www. genpharmtox.com/ services/ Toxicology/micro_advanced_ test.html (28.05.2012)
  • Greywe D, Kreutz J, Banduhn N, Krauledat M, Scheel J, Schroeder KR, Wolf T, Reisinger K, 2012. Applicability and robustness of the hen’s egg test for analysis of micronucleus induction (HET-MN): Results from an inter-laboratory trial. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 747(1): 118-134.
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There are 80 citations in total.

Details

Journal Section Biyoloji / Biology
Authors

Haluk Özparlak

Bülent Çelik This is me

Publication Date December 31, 2016
Submission Date April 4, 2016
Acceptance Date May 16, 2016
Published in Issue Year 2016 Volume: 6 Issue: 4

Cite

APA Özparlak, H., & Çelik, B. (2016). Hen’s Egg Test For Micronucleus Induction (HET-MN). Journal of the Institute of Science and Technology, 6(4), 17-28.
AMA Özparlak H, Çelik B. Hen’s Egg Test For Micronucleus Induction (HET-MN). J. Inst. Sci. and Tech. December 2016;6(4):17-28.
Chicago Özparlak, Haluk, and Bülent Çelik. “Hen’s Egg Test For Micronucleus Induction (HET-MN)”. Journal of the Institute of Science and Technology 6, no. 4 (December 2016): 17-28.
EndNote Özparlak H, Çelik B (December 1, 2016) Hen’s Egg Test For Micronucleus Induction (HET-MN). Journal of the Institute of Science and Technology 6 4 17–28.
IEEE H. Özparlak and B. Çelik, “Hen’s Egg Test For Micronucleus Induction (HET-MN)”, J. Inst. Sci. and Tech., vol. 6, no. 4, pp. 17–28, 2016.
ISNAD Özparlak, Haluk - Çelik, Bülent. “Hen’s Egg Test For Micronucleus Induction (HET-MN)”. Journal of the Institute of Science and Technology 6/4 (December 2016), 17-28.
JAMA Özparlak H, Çelik B. Hen’s Egg Test For Micronucleus Induction (HET-MN). J. Inst. Sci. and Tech. 2016;6:17–28.
MLA Özparlak, Haluk and Bülent Çelik. “Hen’s Egg Test For Micronucleus Induction (HET-MN)”. Journal of the Institute of Science and Technology, vol. 6, no. 4, 2016, pp. 17-28.
Vancouver Özparlak H, Çelik B. Hen’s Egg Test For Micronucleus Induction (HET-MN). J. Inst. Sci. and Tech. 2016;6(4):17-28.