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LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts

Year 2021, Volume: 6 Issue: 3, 294 - 301, 28.09.2021
https://doi.org/10.35229/jaes.864484

Abstract

In the study, the phenolic components and antioxidant activities of edible and nonedible parts of tuber of Arum italicum plant were carried out to determine in methanolic and water extracts. In addition, the antioxidant potential of these parts of the plant was associated with their phenolic composition. Total phenolic content, FRAP, CUPRAC and DPPH tests as radical scavenging activity were performed to determine the antioxidant activity of the plant. The phenolic profiles in the two parts of the tuber were determined in the prepared methanolic extracts by RP–HPLC–UV and LC–MS/MS. The highest values of the total phenolic content, FRAP, CUPRAC and % DPPH were measured in methanolic extracts of nonedible parts of the tuber as 164 µg GAE / mL, 878 TEAC (µM), 0.064 TEAC (µM) and 19.41, respectively. Phenolic profiles of methanolic extracts include rutin, epicatechin, caffeic acid, ferulic acid, p-coumaric acid and vanillin as major components. According to these results, it can be concluded that the nonedible tuber part can be consumed as food.

References

  • Ağalar, H.G., Çiftçi, G. A., Göger, F. & Kırımer, N. (2017). Activity Guided Fractionation of Arum italicum Miller Tubers and the LC/MS-MS Profiles. Records of Natural Products, 12(1), 64-75. DOI: 10.25135/RNP.06.17.05.089
  • Ahmed, H.A.A., Uranbey, S. & Yaman, C. (2018). Efficient in vitro shoot induction of (Arum italicum MILLER) using shoot tips as medicinal and ornamental plant. Mediterranean Agricultural Sciences, 31(3), 295-300. DOI: 10.29136/mediterranean.451778
  • Akbulut, S. & Özkan, Z.C. (2014). Traditional usage of some wild plants in Trabzon Region (Turkey). Kastamonu University Journal of Forestry Faculty, 14(1), 135-145.
  • Apak, R., Güçlü, K., Özyürek, M. & Karademir, S.E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural & Food Chemistry, 52(26), 7970-7981. DOI: 10.1021/jf048741x
  • Arabshahi-Delouee, S. & Urooj, A. (2007). Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chemistry, 102(4), 1233-1240.
  • Benzie, I.F. & Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1), 70-76. DOI: 10.1006/abio.1996.0292
  • Bozyel, M.E., Merdamert-Bozyel, E., Benek, A., Turu, D., Yakan, M.A. & Canlı, K. (2020). Ethnomedicinal Uses of Araceae Taxa in Turkish Traditional Medicine. International Journal of Academic & Applied Research, 4(5), 78-87.
  • Brand-Williams, W., Cuvelier, M.E. & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science & Technology, 28(1), 25-30. DOI: 10.1016/S0023-6438(95)80008-5
  • Cai, Y., Luo, Q., Sun, M. & Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences, 74(17), 2157-2184. DOI: 10.1016/j.lfs.2003.09.047
  • Can, Z., Yildiz, O., Sahin, H., Turumtay, E.A., Silici, S. & Kolayli, S. (2015). An investigation of Turkish honeys: their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chemistry, 180, 133-141. DOI: 10.1016/j.foodchem.2015.02.024
  • Chu, Y.H., Chang, C.L. & Hsu, H.F. (2000). Flavonoid content of several vegetables and their antioxidant activity. Journal of the Science of Food & Agriculture, 80(5), 561-566.
  • de Oliveira Silva, E. & Batista, R. (2017). Ferulic acid and naturally occurring compounds bearing a feruloyl moiety: a review on their structures, occurrence, and potential health benefits. Comprehensive Reviews in Food Science & Food Safety, 16(4), 580-616. DOI: 10.1111/1541-4337.12266
  • Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P. & Vidal, N. (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654-660. DOI: 10.1016/j.foodchem.2005.04.028
  • Erez, M.E., Dalar, A., Fidan, M. & Pınar, S.M. (2019). Comprehensive appraisement of antioxidant potential and phytochemical profile of native botanicals from Turkey. Journal of Food Measurement & Characterization, 13(4), 3230-3241. DOI: 10.1007/s11694-019-00245-x
  • Gidik, B., Akar, Z., Can, Z., Sefali, A. & Erturk, O. (2019). Determination of antioxidant, antimicrobial activities, phenolic compounds of wild Rosa L. species Bayburt, Turkey. Fresenius Environmental Bulletin, 28(12), 9973–9982.
  • Gülcin, I. (2012). Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86(3), 345-391. DOI: 10.1007/s00204-011-0774-2
  • Hirasa, K. & Takemasa, M. (1998). Spice Science and Technology, CRC Press, New York, 232p.
  • Huang, D., Ou, B. & Prior, R. L. (2005). The chemistry behind antioxidant capacity assays. Journal of Agricultural & Food Chemistry, 53(6), 1841-1856. DOI: 10.1021/jf030723c
  • Janbaz, K.H., Saeed, S.A. & Gilani, A.H. (2002). Protective effect of rutin on paracetamol-and CCl4-induced hepatotoxicity in rodents. Fitoterapia, 73(7-8), 557-563. DOI: 10.1016/S0367-326X(02)00217-4
  • Kamalakkannan, N. & Prince, P.S.M. (2006). Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin‐induced diabetic wistar rats. Basic and Clinical Pharmacology & Toxicology, 98(1), 97-103. DOI: 10.1111/j.1742-7843.2006.pto_241.x
  • Karahan, F., Kulak, M., Urlu, E., Gözüacik, H. G., Böyümez, T., Şekeroğlu, N. & Doganturk, I.H. (2015). Total phenolic content, ferric reducing and DPPH scavenging activity of Arum dioscoridis. Natural Product Research, 29(17), 1678-1683. DOI: 10.1080/14786419.2014.991320
  • Lo, H.H. & Chung, J.G. (1999). The effects of plant phenolics, caffeic acid, chlorogenic acid and ferulic acid on arylamine N-acetyltransferase activities in human gastrointestinal microflora. Anticancer Research, 19(1A), 133-139.
  • Mau, J.L., Lin, H.C. & Chen, C.C. (2002). Antioxidant properties of several medicinal mushrooms. Journal of Agricultural & Food Chemistry, 50(21), 6072-6077. DOI: 10.1021/jf0201273
  • Michalak, A. (2006). Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies, 15(4), 523–530.
  • Ng, T.B., Liu, F. & Wang, Z.T. (2000). Antioxidative activity of natural products from plants. Life Sciences, 66(8), 709-723. DOI: 10.1016/S0024-3205(99)00642-6
  • Nilsson, C.L. (1999). Fingerprinting of Helicobacter pylori strains by matrix‐assisted laser desorption/ionization mass spectrometric analysis. Rapid Communications in Mass Spectrometry, 13(11), 1067-1071. DOI: 10.1002/(SICI)1097-0231(19990615)13:11<1067::AID-RCM612>3.0.CO;2-N
  • Odbayar, T.O., Badamhand, D., Kimura, T., Takahashi, Y., Tsushida, T. & Ide, T. (2006). Comparative studies of some phenolic compounds (quercetin, rutin, and ferulic acid) affecting hepatic fatty acid synthesis in mice. Journal of Agricultural & Food Chemistry, 54(21), 8261-8265. DOI: 10.1021/jf061135c
  • Ou, S. & Kwok, K.C. (2004). Ferulic acid: pharmaceutical functions, preparation and applications in foods. Journal of the Science of Food & Agriculture, 84(11), 1261-1269. DOI: 10.1002/jsfa.1873
  • Slinkard, K. & Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1), 49-55.
  • Tosun, G., Arslan, T., Iskefiyeli, Z., Küçük, M., Karaoğlu, Ş.A. & Yayli, N. (2015). Synthesis and biological evaluation of a new series of 4-alkoxy-2-arylquinoline derivatives as potential antituberculosis agents. Turkish Journal of Chemistry, 39(4), 850-866. DOI: 10.3906/kim-1501-112
  • Tsou, M.F., Hung, C.F., Lu, H.F., Wu, L.T., Chang, S.H., Chang, H.L., Chen, G.W. & Chung, J.G. (2000). Effects of caffeic acid, chlorogenic acid and ferulic acid on growth and arylamine N-acetyltransferase activity in Shigella sonnei (group D). Microbios, 101(398), 37-46.
  • Uguzlar, H., Maltas, E. & Yildiz, S. (2012). Screening of phytochemicals and antioxidant activity of Arum dioscoridis seeds. Journal of Food Biochemistry, 36(3), 285-291. DOI: 10.1111/j.1745-4514.2010.00537.x
  • Uttara, B., Singh, A.V., Zamboni, P. & Mahajan, R.T. (2009). Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current Neuropharmacology, 7(1), 65-74. DOI: 10.2174/157015909787602823
  • Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M., & Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The international Journal of Biochemistry & Cell Biology, 39(1), 44-84. DOI: 10.1016/j.biocel.2006.07.001
  • Xiao, C.H., Yang, S.S. & Hong, X.K. (2000). The chemistry of traditional Chinese medicines. Shanghai Science &Technology Publishing House, Shanghai, 36, 505-507.

Arum italicum Miller Bitkisinin Yenilebilir ve Yenmeyen Yumru Parçalarının LC–MS/MS ve RP–HPLC–UV Analizi ve Antioksidan Aktiviteleri

Year 2021, Volume: 6 Issue: 3, 294 - 301, 28.09.2021
https://doi.org/10.35229/jaes.864484

Abstract

Bu çalışmada, Arum italicum bitkisine ait yumrunun yenilebilir ve yenmeyen kısımlarından elde edilen metanolik ve sulu ekstraktların, fenolik içerikleri ve antioksidan aktiviteleri belirlenmiştir. Ek olarak, bitkinin bu kısımlarının antioksidan potansiyeli, fenolik kompoziyonu ile ilişkilendirilmiştir. Bitkinin antioksidan aktivitesini belirlemek için Toplam Fenolik Madde Miktarı, FRAP, CUPRAC ve radikal süpürme aktiviteyi belirleyen DPPH antioksidan aktivite testleri yapılmıştır. Bitki yumrusunun her iki kısmındaki fenolik profiller hazırlanan metanolik ekstraktlarında RP– HPLC–UV ve LC–MS/ MS cihazları kullanılarak belirlenmiştir. En yüksek toplam fenolik içerik, FRAP, CUPRAC ve % DPPH değerleri, yumrunun yenmeyen kısmının metanolik ekstraktında sırasıyla 164 µg GAE/mL, 878 TEAC(µM), 0.064 TEAC(µM) ve 19.41 olarak ölçülmüştür. Metanolik ekstraktların fenolik profilleri, ana bileşenler olarak rutin, epikateşin, kafeik asit, ferulik asit, p-kumarik asit ve vanilin şeklinde belirlenmiştir. Bu sonuçlara göre halk arasında yenmeyen yumru kısmının da besin olarak tüketilebileceği sonucuna varılabilir.

References

  • Ağalar, H.G., Çiftçi, G. A., Göger, F. & Kırımer, N. (2017). Activity Guided Fractionation of Arum italicum Miller Tubers and the LC/MS-MS Profiles. Records of Natural Products, 12(1), 64-75. DOI: 10.25135/RNP.06.17.05.089
  • Ahmed, H.A.A., Uranbey, S. & Yaman, C. (2018). Efficient in vitro shoot induction of (Arum italicum MILLER) using shoot tips as medicinal and ornamental plant. Mediterranean Agricultural Sciences, 31(3), 295-300. DOI: 10.29136/mediterranean.451778
  • Akbulut, S. & Özkan, Z.C. (2014). Traditional usage of some wild plants in Trabzon Region (Turkey). Kastamonu University Journal of Forestry Faculty, 14(1), 135-145.
  • Apak, R., Güçlü, K., Özyürek, M. & Karademir, S.E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural & Food Chemistry, 52(26), 7970-7981. DOI: 10.1021/jf048741x
  • Arabshahi-Delouee, S. & Urooj, A. (2007). Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chemistry, 102(4), 1233-1240.
  • Benzie, I.F. & Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1), 70-76. DOI: 10.1006/abio.1996.0292
  • Bozyel, M.E., Merdamert-Bozyel, E., Benek, A., Turu, D., Yakan, M.A. & Canlı, K. (2020). Ethnomedicinal Uses of Araceae Taxa in Turkish Traditional Medicine. International Journal of Academic & Applied Research, 4(5), 78-87.
  • Brand-Williams, W., Cuvelier, M.E. & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science & Technology, 28(1), 25-30. DOI: 10.1016/S0023-6438(95)80008-5
  • Cai, Y., Luo, Q., Sun, M. & Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences, 74(17), 2157-2184. DOI: 10.1016/j.lfs.2003.09.047
  • Can, Z., Yildiz, O., Sahin, H., Turumtay, E.A., Silici, S. & Kolayli, S. (2015). An investigation of Turkish honeys: their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chemistry, 180, 133-141. DOI: 10.1016/j.foodchem.2015.02.024
  • Chu, Y.H., Chang, C.L. & Hsu, H.F. (2000). Flavonoid content of several vegetables and their antioxidant activity. Journal of the Science of Food & Agriculture, 80(5), 561-566.
  • de Oliveira Silva, E. & Batista, R. (2017). Ferulic acid and naturally occurring compounds bearing a feruloyl moiety: a review on their structures, occurrence, and potential health benefits. Comprehensive Reviews in Food Science & Food Safety, 16(4), 580-616. DOI: 10.1111/1541-4337.12266
  • Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P. & Vidal, N. (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654-660. DOI: 10.1016/j.foodchem.2005.04.028
  • Erez, M.E., Dalar, A., Fidan, M. & Pınar, S.M. (2019). Comprehensive appraisement of antioxidant potential and phytochemical profile of native botanicals from Turkey. Journal of Food Measurement & Characterization, 13(4), 3230-3241. DOI: 10.1007/s11694-019-00245-x
  • Gidik, B., Akar, Z., Can, Z., Sefali, A. & Erturk, O. (2019). Determination of antioxidant, antimicrobial activities, phenolic compounds of wild Rosa L. species Bayburt, Turkey. Fresenius Environmental Bulletin, 28(12), 9973–9982.
  • Gülcin, I. (2012). Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86(3), 345-391. DOI: 10.1007/s00204-011-0774-2
  • Hirasa, K. & Takemasa, M. (1998). Spice Science and Technology, CRC Press, New York, 232p.
  • Huang, D., Ou, B. & Prior, R. L. (2005). The chemistry behind antioxidant capacity assays. Journal of Agricultural & Food Chemistry, 53(6), 1841-1856. DOI: 10.1021/jf030723c
  • Janbaz, K.H., Saeed, S.A. & Gilani, A.H. (2002). Protective effect of rutin on paracetamol-and CCl4-induced hepatotoxicity in rodents. Fitoterapia, 73(7-8), 557-563. DOI: 10.1016/S0367-326X(02)00217-4
  • Kamalakkannan, N. & Prince, P.S.M. (2006). Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin‐induced diabetic wistar rats. Basic and Clinical Pharmacology & Toxicology, 98(1), 97-103. DOI: 10.1111/j.1742-7843.2006.pto_241.x
  • Karahan, F., Kulak, M., Urlu, E., Gözüacik, H. G., Böyümez, T., Şekeroğlu, N. & Doganturk, I.H. (2015). Total phenolic content, ferric reducing and DPPH scavenging activity of Arum dioscoridis. Natural Product Research, 29(17), 1678-1683. DOI: 10.1080/14786419.2014.991320
  • Lo, H.H. & Chung, J.G. (1999). The effects of plant phenolics, caffeic acid, chlorogenic acid and ferulic acid on arylamine N-acetyltransferase activities in human gastrointestinal microflora. Anticancer Research, 19(1A), 133-139.
  • Mau, J.L., Lin, H.C. & Chen, C.C. (2002). Antioxidant properties of several medicinal mushrooms. Journal of Agricultural & Food Chemistry, 50(21), 6072-6077. DOI: 10.1021/jf0201273
  • Michalak, A. (2006). Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies, 15(4), 523–530.
  • Ng, T.B., Liu, F. & Wang, Z.T. (2000). Antioxidative activity of natural products from plants. Life Sciences, 66(8), 709-723. DOI: 10.1016/S0024-3205(99)00642-6
  • Nilsson, C.L. (1999). Fingerprinting of Helicobacter pylori strains by matrix‐assisted laser desorption/ionization mass spectrometric analysis. Rapid Communications in Mass Spectrometry, 13(11), 1067-1071. DOI: 10.1002/(SICI)1097-0231(19990615)13:11<1067::AID-RCM612>3.0.CO;2-N
  • Odbayar, T.O., Badamhand, D., Kimura, T., Takahashi, Y., Tsushida, T. & Ide, T. (2006). Comparative studies of some phenolic compounds (quercetin, rutin, and ferulic acid) affecting hepatic fatty acid synthesis in mice. Journal of Agricultural & Food Chemistry, 54(21), 8261-8265. DOI: 10.1021/jf061135c
  • Ou, S. & Kwok, K.C. (2004). Ferulic acid: pharmaceutical functions, preparation and applications in foods. Journal of the Science of Food & Agriculture, 84(11), 1261-1269. DOI: 10.1002/jsfa.1873
  • Slinkard, K. & Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1), 49-55.
  • Tosun, G., Arslan, T., Iskefiyeli, Z., Küçük, M., Karaoğlu, Ş.A. & Yayli, N. (2015). Synthesis and biological evaluation of a new series of 4-alkoxy-2-arylquinoline derivatives as potential antituberculosis agents. Turkish Journal of Chemistry, 39(4), 850-866. DOI: 10.3906/kim-1501-112
  • Tsou, M.F., Hung, C.F., Lu, H.F., Wu, L.T., Chang, S.H., Chang, H.L., Chen, G.W. & Chung, J.G. (2000). Effects of caffeic acid, chlorogenic acid and ferulic acid on growth and arylamine N-acetyltransferase activity in Shigella sonnei (group D). Microbios, 101(398), 37-46.
  • Uguzlar, H., Maltas, E. & Yildiz, S. (2012). Screening of phytochemicals and antioxidant activity of Arum dioscoridis seeds. Journal of Food Biochemistry, 36(3), 285-291. DOI: 10.1111/j.1745-4514.2010.00537.x
  • Uttara, B., Singh, A.V., Zamboni, P. & Mahajan, R.T. (2009). Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current Neuropharmacology, 7(1), 65-74. DOI: 10.2174/157015909787602823
  • Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M., & Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The international Journal of Biochemistry & Cell Biology, 39(1), 44-84. DOI: 10.1016/j.biocel.2006.07.001
  • Xiao, C.H., Yang, S.S. & Hong, X.K. (2000). The chemistry of traditional Chinese medicines. Shanghai Science &Technology Publishing House, Shanghai, 36, 505-507.
There are 35 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Zeynep Akar 0000-0001-9262-8070

Çağlar Demir This is me 0000-0001-8858-8475

Ozan Alkan This is me 0000-0001-5419-4232

Zehra Can 0000-0002-7156-4941

Bülent Akar 0000-0002-1421-374X

Publication Date September 28, 2021
Submission Date January 19, 2021
Acceptance Date May 23, 2021
Published in Issue Year 2021 Volume: 6 Issue: 3

Cite

APA Akar, Z., Demir, Ç., Alkan, O., Can, Z., et al. (2021). LC–MS/MS and RP–HPLC–UV Analysis and Antioxidant Activities of Arum italicum Miller Edible and Nonedible Tuber Parts. Journal of Anatolian Environmental and Animal Sciences, 6(3), 294-301. https://doi.org/10.35229/jaes.864484


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