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AKIM SİTOMETRİ VE SU ÜRÜNLERİ UYGULAMALARI

Year 2016, Volume: 2 Issue: 4, 159 - 175, 21.06.2016

Metin Yazıcı Remziye Eda Yardımcı

https://doi.org/10.3153/JAEFR16018

Abstract

Akım sitometri (AS), 0.2–150
µm büyüklüğündeki partiküllerin genellikle de hücrelerin tek tek, bir ışık demetinin önünden, bir sıvı
içinde geçerken, anlık olarak ölçümlerini yapan daha sonra da çoklu
fiziksel özelliklerini analiz eden bir teknolojidir. Akım sitometri, hücre biyolojisini
birçok açıdan araştırmak ve istenilen
hücreleri izole etmek için kullanılır. Akım sitometri çok sayıda tek hücrenin birçok karakteristiklerini hızlı bir şekilde
ölçtüğünden hücre tabanlı
analizde altın standart olarak
kabul edilmiştir. Diğer tekniklerle karşılaştırıldığında,
Akım sitometri’nin hızlı veri eldesini ve çok parametreli analizi kolaylaştırması popülaritesinin artmasına ve uygulama alanlarının genişlemesine yol
açmıştır. Bu derlemede, Akım sitometri’nin
su ürünleri alanındaki mevcut durumu ve uygulamaları hakkında özet bir şekilde
bilgi verilmiştir.

Keywords

Akım Sitometri Balıklarda fagositik aktivite Hücre taban-lı analiz Akuatik toksikoloji Plankton Hemosit

References

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  • Bonato, S., Christaki, U., Lefebvre, A., Lizon, F., Thyssen, M. & Artigas, L.F. (2015). High spatial variability of phytoplankton assessed by flow cytometry, in a dynamic productive coastal area, in spring. The Eastern English Channel, Estuarine, Coastal and Shelf Science, 154, 214-223.
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.FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES

Year 2016, Volume: 2 Issue: 4, 159 - 175, 21.06.2016

Metin Yazıcı Remziye Eda Yardımcı

https://doi.org/10.3153/JAEFR16018

Abstract

Flow
cytometry (FCM) is a technology that simultaneously measures and then analyzes
multiple physical characteristics of 0.2–150 µm sized particles, usually cells,
as they flow one by one in a fluid stream through a beam of light. Flow cytometry
is used for investigating many aspects of cell biology and for isolating the
cells desired. Since Flow cytometry measures the multiple characteristics of
large numbers of individual cells rapidly, it has been accepted as the gold
Standard in cell based analysis. Since Flow cytometry facilitates rapid data
acquisition and eases multiparameter analysis, leading to increased popularity
and widespread applications as compared to other analyzing techniques. In this
review, the current status and utilization of Flow cytometry in aquatic sciences
are briefly presented.

Keywords

Flow Cytometry Phagositic activiy of fish Cell-based analysis Aquatic toxicology Plankton Haemocyte

References

  • Alcox, K.A. & Ford, S.E. (1998). Variability in molluscan hemocytes: a flow cytometric study. Tissue Cell, 30(2), 195-204.
  • Allen, S.K. (1983). Flowcytometry: Assaying experimental polyploid fish and shellfish. Aquaculture, 33(1-4), 317-328.
  • Alsharif, R. & Godfrey, W. (2002). Bacterial detection and Live/Dead discrimination by flow cytometry. Immunocytometry systems, BD Biosciences, San Jose, USA.
  • Alver, E., Demirci, A. & Özcimder, M. (2012). Polisiklik Aromatik Hidrokarbonlar ve Sağlığa Etkileri. Mehmet Akif Ersoy Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 3(1), 45-52.
  • Barreda, D.R., Neumann, N.F. & Belosevic, M. (2000). Flow cytometric analysis of PKH26-labele goldfish kidney-derived macrophages. Developmental & Comparative Immunology, 24(4), 395-406.
  • BD Biosciences (2000). Introduction to flow cytometry: A learning guide. Available at: https://tr.scribd.com/doc/184733295/Introduction-to-Flow-Cytometry-A-Learning-Guide (Accessed April 8, 2015).
  • BD Flow Cytometry (2008). Technical Bulletin. https://www.bdbiosciences.com/documents/BD_Research_FlowCyto_TechBulletin.pdf (Accessed May 18, 2015).
  • Bihari, N., Mičić, M., Batel, R. & Zahn, R.K. (2003). Flow cytometric detection of DNA cell cycle alterations in hemocytes of mussels (Mytilus galloprovincialis) off the Adriatic coast, Croatia. Aquatic Toxicology, 64(2), 121-129.
  • Bonato, S., Christaki, U., Lefebvre, A., Lizon, F., Thyssen, M. & Artigas, L.F. (2015). High spatial variability of phytoplankton assessed by flow cytometry, in a dynamic productive coastal area, in spring. The Eastern English Channel, Estuarine, Coastal and Shelf Science, 154, 214-223.
  • Chaiton, J.A. & Allen, S.K. (1985). Early detection of triploidy in the larvae of Pacific oysters, Crassostrea gigas, by flow cytometry. Aquaculture, 48(1), 35-43.
  • Chilmonczyk, S. & Monge, D. (1999). Flow cytometry as a tool for assessment of the fish cellular immune response to pathogens. Fish & Shellfish Immunology, 9(4), 319-333.
  • Chilmonczyk, S. & Monge, D. (1998). Cellular Immune Response in Rainbow Trout (Oncorhynchus mykiss): Flow Cytometric Study. Acta Veterinaria Brno, 67, 207-213.
  • Cid, A., Herrero, C., Torres, E. & Abalde, J. (1995). Copper toxicity on the marine microalga Phaeodactylum tricornutum: effects on photosynthesis and related parameters. Aquatic Toxicology, 31(2), 165-174.
  • Corina, P. & Brussaard, D. (2004). Optimization of Procedures for Counting Viruses by Flow Cytometry. Applied and Environmental Microbiology, 70(3), 1506-1513.
  • Cuesta, A., Esteban, M.A. & Meseguer, J. (1999). Natural cytotoxic activity of gilthead seabream (Sparus aurata L.) leucocytes: Assessment by flow cytometry and microscopy. Veterinary Immunology and Immunopathology, 71(3–4), 161–171.
  • Czechowska, K., Johnson, D.R. & Van der Meer, J.R. (2008): Use of flow cytometric methods for single-cell analysis in environmental microbiology. Current Opinion in Microbiology, 11, 205–212.
  • Danion, M., Le Floch, S., Kanan, R., Lamour, F. & Quentel, C. (2011). Effects of in vivo chronic hydrocarbons pollution on sanitary status and immune system in sea bass (Dicentrarchus labrax L.). Aquatic Toxicology, 105(3-4), 300-11.
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There are 89 citations in total.

Details

Subjects Hydrobiology
Journal Section Articles
Authors

Metin Yazıcı

Remziye Eda Yardımcı

Publication Date June 21, 2016
Published in Issue Year 2016 Volume: 2 Issue: 4

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APA Yazıcı, M., & Yardımcı, R. E. (2016). .FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES. Journal of Aquaculture Engineering and Fisheries Research, 2(4), 159-175. https://doi.org/10.3153/JAEFR16018
AMA Yazıcı M, Yardımcı RE. .FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES. J Aquacult Eng Fish Res. October 2016;2(4):159-175. doi:10.3153/JAEFR16018
Chicago Yazıcı, Metin, and Remziye Eda Yardımcı. “.FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES”. Journal of Aquaculture Engineering and Fisheries Research 2, no. 4 (October 2016): 159-75. https://doi.org/10.3153/JAEFR16018.
EndNote Yazıcı M, Yardımcı RE (October 1, 2016) .FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES. Journal of Aquaculture Engineering and Fisheries Research 2 4 159–175.
IEEE M. Yazıcı and R. E. Yardımcı, “.FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES”, J Aquacult Eng Fish Res, vol. 2, no. 4, pp. 159–175, 2016, doi: 10.3153/JAEFR16018.
ISNAD Yazıcı, Metin - Yardımcı, Remziye Eda. “.FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES”. Journal of Aquaculture Engineering and Fisheries Research 2/4 (October 2016), 159-175. https://doi.org/10.3153/JAEFR16018.
JAMA Yazıcı M, Yardımcı RE. .FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES. J Aquacult Eng Fish Res. 2016;2:159–175.
MLA Yazıcı, Metin and Remziye Eda Yardımcı. “.FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES”. Journal of Aquaculture Engineering and Fisheries Research, vol. 2, no. 4, 2016, pp. 159-75, doi:10.3153/JAEFR16018.
Vancouver Yazıcı M, Yardımcı RE. .FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES. J Aquacult Eng Fish Res. 2016;2(4):159-75.
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