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Experimental Toxoplasmosis in White Turkeys

Year 2017, Volume: 43 Issue: 2, 123 - 131, 16.06.2017
https://doi.org/10.16988/iuvfd.321873

Abstract

In this
study, forming of experimental toxoplasmosis in white turkeys; clinical,
pathological and serological determination of tissue lesions, aimed to compare
them and determine pathogenesis. For these purposes, a total of 120 two months
old white turkeys were divided into groups as oral and parenteral infections
and also their controls. The oral group was infected with 0.5 mL of Toxoplasma
gondii RH strain inoculum suspension contains 10
6 tachyzoites while
its control group was given 0.5 mL of saline. The parenteral group were divided
into four groups as intraperitoneal (IP), intramuscular (IM), intravenous (IV)
and cloacal (C). Each parenteral route was divided into two groups and one
control group for inoculums given 10
5 and 104 tachyzoites
in 0.5 mL. These two control groups were also given 0.5 mL saline as indicated
above. Due to acute toxoplasmosis, death occurred in three white turkeys given
tachyzoites IP 10
5 showed neurological clinical symptoms as
torticollis, ataxia, and tremor. In the histopahologic examination of these
three turkeys, T. gondii tissue cysts were detected in the brain. Also, one of
given 10
5 tachyzoites IP group in the brain and one given 104
tachyzoites IV group in the liver were observed tissue cysts associated with
toxoplasmosis. The turkeys in all infection groups were found seropositive in
both Sabin-Feldman Dye Test (SFDT) and Indirect Hemagglutination Test (IHA).
The statistical difference between SFDT and IHA was insignificantly for the
both parenteral infection groups (P>0.05) while the difference was found
significantly for the orally infected group (P<0.05). In conclusion in the
present study, the tissue cysts of T. gondii were microscopically seen in brain
and liver of the experimental infected white turkeys.



 

References

  • Abbas, A.M.A., 1967. Toxoplasmosis of chickens experimentally infected during embryonic life. Transactions of the Royal Society of Tropical Medicine and Hygiene 61, 514-516.
  • Altınöz, F., Babür, C., Kılıç, S., 2007. Konya Yöresinde Yumurta Tavuklarında Sabin-Feldman boya testi ile Toxoplasma gondii (Nicolle ve Manceaux, 1908) seropozitifliğinin araştırılması. Türkiye Parazitoloji Dergisi 31, 4-6.
  • Babür, C., Gıcık, Y., İnci, A., 1998. Ankara’da güvercinlerde Sabin-Feldman boya testi ile anti-Toxoplasma gondii antikorlarının araştırılması. Türkiye Parazitoloji Dergisi 22, 308-10.
  • Bangoura, B., Zöller, B., Koethe, M., Ludewig, M., Pott, S., Fehlhaber, K., Daugschies, A., 2013. Experimental Toxoplasma gondii oocyst infections in turkeys (Meleagris gallopavo). Veterinary Parasitology 196, 272-277.
  • Bickford, A.A., Saunders, J.R., 1966. Experimental toxoplasmosis in chickens. American Journal of Veterinary Research 27, 308-318.
  • Devada, K., Anandan, R., Dubey, J. P., 1998. Serologic Prevalence Of Toxoplasma Gondii in Chickens In Madras, India. The Journal Of Parasitology 84, 621-622.
  • Dubey, J.P., 2002. A Review Of Toxoplasmosis in Wild Birds. Veterinary Parasitology 106, 121-153.
  • Dubey, J.P., Ruff, M.D., Kwok, O.C.H., Shen, S.K., Wilkins, G.C., Thulliez, P., 1993a. Experimental Toxoplasmosis in Bobwhite Quail (Colinus Virginianus). The Journal Of Parasitology 79, 935-939.
  • Dubey, J.P., Camargo, M.E., Ruff, M.D., Wilkins, G.C., Shen, S. K., Kwok, O.C.H., Thulliez, P., 1993b. Experimental Toxoplasmosis in Turkeys. The Journal Of Parasitology 79, 949-952.
  • Dubey, J.P., Ruff, M.D., Camargo, M.E., Shen, S. K., Wilkins, G.L., Kwok, O.C., Thulliez, P., 1993c. Serologic and parasitologic responses of domestic chickens after oral inoculation with Toxoplasma Gondii oocysts. American Journal Of Veterinary Research 54, 1668-1672.
  • Dubey, J.P., Goodwin, M.A., Ruff, M.D., Kwok, O.C.H., Shen, S. K., Wilkins, G.C., Thulliez, P., 1994a. Experimental Toxoplasmosis in Japanese Quail. Journal Of Veterinary Diagnostic Investigation 6, 216-221.
  • Dubey, J.P., Ruff, M.D., Wilkins, G.C., Shen, S.K., Kwok, O.C. H., 1994b. Experimental Toxoplasmosis in Pheasants (Phasianus Colchicus). Journal Of Wildlife Diseases 30, 40-45.
  • El-Massry, A., Mahdy, O.A., El-Ghaysh, A., Dubey, J.P., 2000. Prevalence Of Toxoplasma Gondii Antibodies in Sera Of Turkeys, Chickens, And Ducks From Egypt. Journal Of Parasitology 86, 627-628.
  • Howerth, E.W., Rodenroth, N., 1985. Fatal systemic toxoplasmosis in a wild turkey. Journal Of Wildlife Diseases 21, 446-449.
  • İnci, A., Babür, C., Dinçer, S., Erdal, N., 1998. Türkiye’nin bazı illerinde evcil kanatlılarda Sabin–Feldman boya testi ile anti-toxoplasma gondii antikorlarının saptanması. Acta Parasitologica Turcica 22, 420-423.
  • İnci, A., Babür, C., Çam, Y., İça, A., 2002a. Kayseri yöresinde bazı yırtıcı kuşlarda Sabin-Feldman boya testi Toxoplasma Gondii (Nicole Ve Manceaux, 1908) seropozitifliğinin araştırılması. Fırat Üniversitesi Sağlık Bilimleri Dergisi 16, 177-179.
  • İnci, A., Babür, C., İşcan, K.M., İça, A., 2002b. Bıldırcınlarda (Coturnix Coturnix Japonica) Toxoplasma Gondii (Nicolle Ve Manceaux, 1908) spesifik antikorlarının Sabin-Feldman boya testi ile araştırılması. Türkiye Parazitoloji Dergisi 26, 20-22.
  • Jacobs, L., Melton, M.L., 1966. Toxoplasmosis in chickens. The Journal Of Parasitology 52, 1158-1162.
  • Kaneto, C.N., Costa, A.J., Paulillo, A.C., Moraes, F.R., Murakami, T.O., Meireles, M.V., 1997. Experimental toxoplasmosis in broiler chicks. Veterinary Parasitology 69, 203-210.
  • Kinjo, T., 1961. Studies on experimental toxoplasmosis in chickens. Japanese Journal Of Veterinary Research 9, 125-126.
  • Kirkpatrick, C.E., Colvin, B.A., Dubey, J.P., 1990. Toxoplasma Gondii antibodies in common barn-owls (Tyto Alba) and pigeons (Columba Livia) in New Jersey. Veterinary Parasitology 36, 177-180.
  • Lindsay, D.S., Smith, P.C., Blagburn, B.L., 1994. Prevalence and isolation of Toxoplasma gondii from wild turkeys in Alabama. Journal of Helminthological Society of Washington 61, 115-117.
  • Mason, R.W., Hartley, W.J., Dubey, J.P., 1991. Lethal toxoplasmosis in a little penguin (Eudyptula Minor) from Tasmania. The Journal Of Parasitology 77, 328.
  • Muz, M.N., Kılınç, Ö.O., İşler, C.T., 2015. Bazı Yabani Kuşların Beyin Dokularında Toxoplasma Gondii Ve Neospora Caninum’un Moleküler Tanısı. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, 173-178.
  • Quist, C.F., Dubey, J.P., Luttrell, M.P., Davidson, W.R., 1995. Toxoplasmosis in wild turkeys: A case report and serologic survey. Journal Of Wildlife Diseases 31, 255-258.
  • Siim, J.C., Biering-Sørensen, U., Møller T., 1963. Toxoplasmosis in Domestic Animals. Advances in Veterinary Science 8, 335-429.
  • Tenter, A.M., Heckeroth, A.R., Weiss, L.M., 2000. Toxoplasma Gondii: From animals to humans. International Journal For Parasitology 30, 1217-1258.
  • Vickers, M.C., Hartley, W.J., Mason, R.W., Dubey, J.P., Schollam, L., 1992. Blindness Associated With Toxoplasmosis in Canaries. Journal Of The American Veterinary Medical Association 200, 1723-1725.
  • Williams, S.M., Fulton, R.M., Render, J.A., Mansfield, L., Bouldin, M., 2001. Ocular and encephalic toxoplasmosis in canaries. Avian Diseases 45, 262-267.
  • Zöller, B., Koethe, M., Ludewig, M., Pott, S., Daugschies, A., Straubinger, R.K., Bangoura, B., 2013. Tissue tropism of Toxoplasma gondii in turkeys (Meleagris gallopavo) after parenteral infection. Parasitology Research 112, 1841-1847.

Beyaz Hindilerde Deneysel Toksoplazmozis

Year 2017, Volume: 43 Issue: 2, 123 - 131, 16.06.2017
https://doi.org/10.16988/iuvfd.321873

Abstract

Bu çalışmada, beyaz
hindilerde deneysel toxoplasmozis oluşturulması; klinik, patolojik ve serolojik
teknikler kullanılarak doku lezyonlarının belirlenmesi, bunların
karşılaştırılması ve patogenezi amaçlanmıştır. Çalışmada, 2 aylık yaşta 120
adet beyaz hindi oral ve parenteral yollarla ve aynı zamanda onların kontrol
grupları olarak gruplandırılmıştır. Oral gruba 0,5 mL’sinde 10
6
takizoit içeren inokulum verilirken bunun kontrol grubuna 0,5 mL serum
fizyolojik verilmiştir. Parenteral grup; İntravenöz (İV), İntraperitoneal (İP),
İntramuskuler (İM), ve Kloakal (K) olarak dört gruba ayrılmıştır. Her bir parenteral
yol da 0,5 mL’sinde 10
5 ve 104 takizoit içeren inokulum
verilmesi için kendi içerisinde iki gruba ve birer kontrol grubuna ayrılmıştır.
Bunların kontrol gruplarınada 0,5 mL serum fizyolojik verilmiştir. İP 10
5
takizoit verilen grubtaki üç hindide akut toxoplazmozise bağlı tortikollis,
ataksi ve tremor gibi sinirsel klinik belirtileri takiben ölüm şekillenmiştir.
Bu hindilerin histopatolojisinde beyinde doku kistleri izlenmiştir. Ayrıca İP
10
5 takizoit verilen gruptaki bir hindide beyinde, İV 104
takizoit verilen grubtaki bir hindide karaciğerde toksoplasmozis’e ilişkin doku
kisti görülmüştür. Sabin-Feldman Dye Test (SFDT) ve İndirekt Hemaglutinasyon (İHA)
ile 10
4 ve 105 takizoit gruplarında seropozitiflik
belirlenmiştir. SFDT ve İHA arasındaki istatistiksel fark önemsiz bulunmuştur
(P>0,05). 10
6 takizoit verilerek enfekte edilen oral enfeksiyon
grubunda SFDT ve İHA arasında istatistiksel farklılık önemli bulunmuştur. (P<0,05).
Sonuç olarak bu çalışmada deneysel enfekte edilen beyaz hindilerde mikroskopik
olarak
T. gondii doku kistleri beyin
ve karaciğerde görülmüştür.

References

  • Abbas, A.M.A., 1967. Toxoplasmosis of chickens experimentally infected during embryonic life. Transactions of the Royal Society of Tropical Medicine and Hygiene 61, 514-516.
  • Altınöz, F., Babür, C., Kılıç, S., 2007. Konya Yöresinde Yumurta Tavuklarında Sabin-Feldman boya testi ile Toxoplasma gondii (Nicolle ve Manceaux, 1908) seropozitifliğinin araştırılması. Türkiye Parazitoloji Dergisi 31, 4-6.
  • Babür, C., Gıcık, Y., İnci, A., 1998. Ankara’da güvercinlerde Sabin-Feldman boya testi ile anti-Toxoplasma gondii antikorlarının araştırılması. Türkiye Parazitoloji Dergisi 22, 308-10.
  • Bangoura, B., Zöller, B., Koethe, M., Ludewig, M., Pott, S., Fehlhaber, K., Daugschies, A., 2013. Experimental Toxoplasma gondii oocyst infections in turkeys (Meleagris gallopavo). Veterinary Parasitology 196, 272-277.
  • Bickford, A.A., Saunders, J.R., 1966. Experimental toxoplasmosis in chickens. American Journal of Veterinary Research 27, 308-318.
  • Devada, K., Anandan, R., Dubey, J. P., 1998. Serologic Prevalence Of Toxoplasma Gondii in Chickens In Madras, India. The Journal Of Parasitology 84, 621-622.
  • Dubey, J.P., 2002. A Review Of Toxoplasmosis in Wild Birds. Veterinary Parasitology 106, 121-153.
  • Dubey, J.P., Ruff, M.D., Kwok, O.C.H., Shen, S.K., Wilkins, G.C., Thulliez, P., 1993a. Experimental Toxoplasmosis in Bobwhite Quail (Colinus Virginianus). The Journal Of Parasitology 79, 935-939.
  • Dubey, J.P., Camargo, M.E., Ruff, M.D., Wilkins, G.C., Shen, S. K., Kwok, O.C.H., Thulliez, P., 1993b. Experimental Toxoplasmosis in Turkeys. The Journal Of Parasitology 79, 949-952.
  • Dubey, J.P., Ruff, M.D., Camargo, M.E., Shen, S. K., Wilkins, G.L., Kwok, O.C., Thulliez, P., 1993c. Serologic and parasitologic responses of domestic chickens after oral inoculation with Toxoplasma Gondii oocysts. American Journal Of Veterinary Research 54, 1668-1672.
  • Dubey, J.P., Goodwin, M.A., Ruff, M.D., Kwok, O.C.H., Shen, S. K., Wilkins, G.C., Thulliez, P., 1994a. Experimental Toxoplasmosis in Japanese Quail. Journal Of Veterinary Diagnostic Investigation 6, 216-221.
  • Dubey, J.P., Ruff, M.D., Wilkins, G.C., Shen, S.K., Kwok, O.C. H., 1994b. Experimental Toxoplasmosis in Pheasants (Phasianus Colchicus). Journal Of Wildlife Diseases 30, 40-45.
  • El-Massry, A., Mahdy, O.A., El-Ghaysh, A., Dubey, J.P., 2000. Prevalence Of Toxoplasma Gondii Antibodies in Sera Of Turkeys, Chickens, And Ducks From Egypt. Journal Of Parasitology 86, 627-628.
  • Howerth, E.W., Rodenroth, N., 1985. Fatal systemic toxoplasmosis in a wild turkey. Journal Of Wildlife Diseases 21, 446-449.
  • İnci, A., Babür, C., Dinçer, S., Erdal, N., 1998. Türkiye’nin bazı illerinde evcil kanatlılarda Sabin–Feldman boya testi ile anti-toxoplasma gondii antikorlarının saptanması. Acta Parasitologica Turcica 22, 420-423.
  • İnci, A., Babür, C., Çam, Y., İça, A., 2002a. Kayseri yöresinde bazı yırtıcı kuşlarda Sabin-Feldman boya testi Toxoplasma Gondii (Nicole Ve Manceaux, 1908) seropozitifliğinin araştırılması. Fırat Üniversitesi Sağlık Bilimleri Dergisi 16, 177-179.
  • İnci, A., Babür, C., İşcan, K.M., İça, A., 2002b. Bıldırcınlarda (Coturnix Coturnix Japonica) Toxoplasma Gondii (Nicolle Ve Manceaux, 1908) spesifik antikorlarının Sabin-Feldman boya testi ile araştırılması. Türkiye Parazitoloji Dergisi 26, 20-22.
  • Jacobs, L., Melton, M.L., 1966. Toxoplasmosis in chickens. The Journal Of Parasitology 52, 1158-1162.
  • Kaneto, C.N., Costa, A.J., Paulillo, A.C., Moraes, F.R., Murakami, T.O., Meireles, M.V., 1997. Experimental toxoplasmosis in broiler chicks. Veterinary Parasitology 69, 203-210.
  • Kinjo, T., 1961. Studies on experimental toxoplasmosis in chickens. Japanese Journal Of Veterinary Research 9, 125-126.
  • Kirkpatrick, C.E., Colvin, B.A., Dubey, J.P., 1990. Toxoplasma Gondii antibodies in common barn-owls (Tyto Alba) and pigeons (Columba Livia) in New Jersey. Veterinary Parasitology 36, 177-180.
  • Lindsay, D.S., Smith, P.C., Blagburn, B.L., 1994. Prevalence and isolation of Toxoplasma gondii from wild turkeys in Alabama. Journal of Helminthological Society of Washington 61, 115-117.
  • Mason, R.W., Hartley, W.J., Dubey, J.P., 1991. Lethal toxoplasmosis in a little penguin (Eudyptula Minor) from Tasmania. The Journal Of Parasitology 77, 328.
  • Muz, M.N., Kılınç, Ö.O., İşler, C.T., 2015. Bazı Yabani Kuşların Beyin Dokularında Toxoplasma Gondii Ve Neospora Caninum’un Moleküler Tanısı. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, 173-178.
  • Quist, C.F., Dubey, J.P., Luttrell, M.P., Davidson, W.R., 1995. Toxoplasmosis in wild turkeys: A case report and serologic survey. Journal Of Wildlife Diseases 31, 255-258.
  • Siim, J.C., Biering-Sørensen, U., Møller T., 1963. Toxoplasmosis in Domestic Animals. Advances in Veterinary Science 8, 335-429.
  • Tenter, A.M., Heckeroth, A.R., Weiss, L.M., 2000. Toxoplasma Gondii: From animals to humans. International Journal For Parasitology 30, 1217-1258.
  • Vickers, M.C., Hartley, W.J., Mason, R.W., Dubey, J.P., Schollam, L., 1992. Blindness Associated With Toxoplasmosis in Canaries. Journal Of The American Veterinary Medical Association 200, 1723-1725.
  • Williams, S.M., Fulton, R.M., Render, J.A., Mansfield, L., Bouldin, M., 2001. Ocular and encephalic toxoplasmosis in canaries. Avian Diseases 45, 262-267.
  • Zöller, B., Koethe, M., Ludewig, M., Pott, S., Daugschies, A., Straubinger, R.K., Bangoura, B., 2013. Tissue tropism of Toxoplasma gondii in turkeys (Meleagris gallopavo) after parenteral infection. Parasitology Research 112, 1841-1847.
There are 30 citations in total.

Details

Subjects Health Care Administration
Journal Section Research Article
Authors

Ayhan Atasever

Cahit Babür

Abdullah İnci

Görkem Ekebaş

Anıl İça

Publication Date June 16, 2017
Published in Issue Year 2017 Volume: 43 Issue: 2

Cite

APA Atasever, A., Babür, C., İnci, A., Ekebaş, G., et al. (2017). Beyaz Hindilerde Deneysel Toksoplazmozis. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 43(2), 123-131. https://doi.org/10.16988/iuvfd.321873
AMA Atasever A, Babür C, İnci A, Ekebaş G, İça A. Beyaz Hindilerde Deneysel Toksoplazmozis. iuvfd. July 2017;43(2):123-131. doi:10.16988/iuvfd.321873
Chicago Atasever, Ayhan, Cahit Babür, Abdullah İnci, Görkem Ekebaş, and Anıl İça. “Beyaz Hindilerde Deneysel Toksoplazmozis”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 43, no. 2 (July 2017): 123-31. https://doi.org/10.16988/iuvfd.321873.
EndNote Atasever A, Babür C, İnci A, Ekebaş G, İça A (July 1, 2017) Beyaz Hindilerde Deneysel Toksoplazmozis. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 43 2 123–131.
IEEE A. Atasever, C. Babür, A. İnci, G. Ekebaş, and A. İça, “Beyaz Hindilerde Deneysel Toksoplazmozis”, iuvfd, vol. 43, no. 2, pp. 123–131, 2017, doi: 10.16988/iuvfd.321873.
ISNAD Atasever, Ayhan et al. “Beyaz Hindilerde Deneysel Toksoplazmozis”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 43/2 (July 2017), 123-131. https://doi.org/10.16988/iuvfd.321873.
JAMA Atasever A, Babür C, İnci A, Ekebaş G, İça A. Beyaz Hindilerde Deneysel Toksoplazmozis. iuvfd. 2017;43:123–131.
MLA Atasever, Ayhan et al. “Beyaz Hindilerde Deneysel Toksoplazmozis”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, vol. 43, no. 2, 2017, pp. 123-31, doi:10.16988/iuvfd.321873.
Vancouver Atasever A, Babür C, İnci A, Ekebaş G, İça A. Beyaz Hindilerde Deneysel Toksoplazmozis. iuvfd. 2017;43(2):123-31.