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Metal Bioaccumulation of Mytilaster lineatus (Gmelin, 1791) Collected From Sinop Coast in the Southern Black Sea

Year 2019, Volume: 78 Issue: 1, 23 - 28, 31.05.2019

Levent Bat Fatih Sahin Aysah Oztekin

Abstract

ABSTRACT
Objective: Metal bioaccumulation of the bivalve Mytilaster lineatus, based on different seasons and stations was investigated in Sinop Peninsula of the Black Sea. Soft tissues of mussels from Gazibey Rock and Içliman were taken during the spring and the summer of the year 2016 and were analyzed for Hg, Cd, Pb, Cu and Zn. Methods: The soft tissues of the mussels digested with Suprapur® HNO3 (nitric acid) using a Milestone Systems, Start D 260 microwave digestion system. The accuracy and precision of the method was verified by the simultaneous analysis of the certified reference materials NIST 2976 (mussel tissue) for the samples. The Inductively Coupled Plasma–Mass Spectrometer (ICP-MS), Agilent Technologies, 7700x was used for metal analyses.   Results: Results showed that higher concentrations of almost all metals in Içliman than those in Gazibey Rock.  However, no significant differences were detected between seasons except for both Cu and Zn. The metal amounts of M. lineatus ranged between 0.024-0.035, 0.054-0.072, 0.13-0.25, 0.64-0.85 and 6-20 mg kg-1 wet wt. for Hg, Cd, Pb, Cu and Zn, respectively. Conclusions: The measured metals in both sampling areas did not exceed the threshold set by the European Commission and Turkish Food Codex. The results of this study also confirmed the potential of M. lineatus to be used as bio-indicators of heavy metal pollution. 

Keywords

Heavy metals bioaccumulation bio-indicator Mytilaster lineatus Black Sea

Thanks

The authors wish to acknowledge the Department of Hydrobiology, Fisheries Faculty, University of Sinop for providing laboratory facilities during the study.

References

  • 1. Bat L, Öztekin A, Şahin F, Arici E, Özsandikci U. An overview of the Black Sea pollution in Turkey. MedFAR 2018; 1(2): 67-86.
  • 2. Bat L, Özkan EY. Chapter 13. Heavy metal levels in sediment of the Turkish Black Sea coast. In: Bikarska I, Raykov V, Nikolov N. (Eds.) Progressive Engineering Practices in Marine Resource Management. IGI Global book series Advances in Environmental Engineering and Green Technologies (AEEGT) (ISSN: 2326-9162; eISSN: 2326-9170) USA, 2016; 399-419.
  • 3. Bat L, Arıcı E. Chapter 5. Heavy Metal Levels in Fish, Molluscs, and Crustacea From Turkish Seas and Potential Risk of Human Health. In: Holban AM, Grumezescu AM. (Eds.) Handbook of Food Bioengineering, Volume 13, Food Quality: Balancing Health and Disease. Elsevier, Academic Press, ISBN: 978-0-12-811442-1, 2018 ; 159-196. http://dx.doi.org/10.1016/B978-0-12-811442-1.00005-5
  • 4. Phillips DJH, Rainbow PS. Biomonitoring of trace aquatic contaminants. Environmental Management Series, Chapman & Hall, London 1994.
  • 5. Phillips DJH. The chemistries and environmental fates of trace metals and organochlorines in aquatic ecosystems. Mar Pollut Bull 1995; 31(4-12): 193-00.
  • 6. Uysal H. Türkiye sahillerinde bulunan midyeler ‘Mytilus galloprovincialis Lamark’ üzerinde biyolojik ve ekolojik araştırmalar. EÜ Fen Fak İlmi Rap Ser 1970; 79: 1-79.
  • 7. Çulha M, Bat L, Türk-Çulha S, Yeşim-Çelik M. Benthic mollusk composition of some facies in the upper-infralittoral zone of the southern Black Sea, Turkey. Turk J Zool 2010; 34: 523-32.
  • 8. Bat L, Gönlügür G. Sinop Sahillerinin üst-infralittoral zonundaki midye fasiesleri üzerinde kalitatif ve kantitatif araştırmalar. (Qualitative and quantitative investigations on mussel facies in the upper-infralittoral zone of Sinop coasts, Black Sea, Turkey). (OMÜ S.057 Numaralı Araştırma Fonu) 2001; 31 pp.
  • 9. Mülayim A, Balkis H. Toxic Metal (Pb, Cd, Cr, and Hg) levels in Rapana venosa (Valenciennes, 1846), Eriphia verrucosa (Forskal, 1775), and sediment samples from the Black Sea littoral (Thrace, Turkey). Mar Poll Bull 2015; 95: 215-22.
  • 10. Bat L, Öztekin HC. Heavy Metals in Mytilus galloprovincialis, Rapana venosa and Eriphia verrucosa from the Black Sea coasts of Turkey as bioindicators of pollution. Walailak J Sci Tech 2016; 13(9): 715-28.
  • 11. Bat L, Arici E, Öztekin A. Human health risk assessment of heavy metals in the Black Sea: evaluating mussels. Current World Environ 2018; 13(1): 15-31.
  • 12. Bat L, Arici E, Öztekin A, Yardim Ö, Üstün F. Use of the Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 from Sinop coasts of the Black Sea as bio-monitor. Int J Mar Sci 2018; 8(5): 44-7.
  • 13. Bat L, Şahin F, Öztekin A. Toxic elements in edible Mollusks from Igneada coasts of the Black Sea, Turkey. Korean J Food Health Conv 2018; 4(3): 22-31. doi: http://dx.doi.org/10.13106/kjfhc.2018.vol4.no3.22
  • 14. Bat L, Sezgin M, Şahin F, Birinci Özdemir Z, Ürkmez D. Sinop city fishery of the Black Sea. Mar Sci 2013; 3(3): 55-64. DOI: 10.5923/j. ms.20130303.01
  • 15. Bat L, Bat L, Sahin F, Sezgin M, Gonener S, Erdem E, Ozsandıkcı U. Fishery of Sinop coasts in the Black Sea surveys. Eur J Biol 2018; 77(1): 18-25. doi: 10.26650/EuroJBiol.2018.388175
  • 16. Bernhard M. Manual of methods in the aquatic environment research.Rome: Food and Agriculture Organization. 1976.
  • 17. United Nations Environment Programme. Reference methods for marine pollution studies. RSMS 11. Determination of total cadmium, zinc, lead and copper in selected marine organisms by flameless atomic absorption spectrophotometry. Geneva: United Nations Environment Programme; 1984. [Online] Available from: http://www.unep.ch/regionalseas/pubs/rsrm.htm [Accessed on 22nd November, 2016]
  • 18. United Nations Environment Programme. GESAMP: Cadmium, lead and tin the marine environment. UNEP Regional Seas Reports and Studies No. 56. Nairobi: United Nations Environment Programme; 1985. [Online] Available from: http://www.unep.org/regionalseas/ publications/reports/RSRS/pdfs/rsrs056.pdf [Accessed on 1th December, 2016]
  • 19. Zar JH. Biostatistical analysis. 2nd ed. New Jersey: Prentice Hall 1984.
  • 20. MAFF (The Ministry of Agriculture, Forestry and Fisheries). Monitoring and surveillance of non-radioactive contaminants in the aquatic environment and activities regulating the disposal wastes at sea, of 1993. Directorate of Fisheries research, Lowestoft, Aquatic Environment Monitoring Report, 1995; 44.
  • 21. TFC. Official Gazette of Republic of Turkey. Notifications about determination of the maximum levels for certain contaminants in foodstuffs of Turkish Food Codex (inTurkish). (Notification No: 2002/63) 2002; 24885.
  • 22. Council of Europe. Partial Agreement in the Social and Public Field. The Guidelines on Metals and Alloys used as Food Contact Materials are Part of the Council of Europe’s Policy Statements Concerning Materials and Articles Intented to Come into Contact with Foodstuffs. Technical Document Strasbourg, 2001.
  • 23. Commission Regulation (EC). Setting maximum levels for certain contaminants in foodstuffs. 2006; 1881.
  • 24. TFC. Official Gazette of Republic of Turkey. Notifications changes to the maximum levels for certain contaminants in foodstuffs (in Turkish). (Notification No: 2009/22) 2009; 27143.
  • 25. Beltrame MO, De Marco SG, Marcovecchio JE. Influences of sex, habitat, and seasonality on heavy-metal concentrations in the burrowing crab (Neohelice granulata) from a coastal lagoon in Argentina. Arch Environ Contam Toxicol 2010; 58(3): 746-56. doi: 10.1007/s00244-009-9405-9
  • 26. Simonetti P, Botté SE, Fiori SM, Marcovecchio JE. Heavy-metal concentrations in soft tissues of the burrowing crab Neohelice granulata in Bahía Blanca Estuary, Argentina. Arch Environ Contam Toxicol 2012; 62: 243–253. doi: 10.1007/s00244-011-9692-9
  • 27. Baltas H, Sirin M, Dalgic G, Bayrak EY, Akdeniz A. Assessment of metal concentrations (Cu, Zn, and Pb) in seawater, sediment and biota samples in the coastal area of eastern Black Sea, Turkey. Mar Pollut Bull 2017; 122(1-2): 475-82. doi: 10.1016/j.marpolbul.2017.06.059
  • 28. Demina LL, Budko DM. Trace metals in carbonate biomineralization by the example of Bivalvia Mytilus spp. from the Black Sea. (in Russian) Geologo-Mineralogical Sci Fundamental Res 2014; 11: 2185-89.
  • 29. Temerdashev ZA, Eletskii II, Kaunova AA, Korpakova IG. Determination of heavy metals in Mytilus galloprovincialis Lamarck mussels using the IСP-AES method. (in Russian). Аналитика и контроль 2017; 21(2): 116-24.
  • 30. Egorov VN, Lazorenko GE, Mirzoyeva NY, Stokozov NA, Kostova SK, Malakhova LV, Pirkova AV, Arkhipova SI, Korkishko NF, Popovichev VN, Plotitsyna OV, Migal LV. Content of 137 Cs, 40 K, 90 Sr, 210 Po radionuclides and some chemical pollutants in the Black Sea mussels, Mytilus galloprovincialis Lam. Морской экологический журнал 2006; 5(3): 70-8.
  • 31. Strungaru SA, Nicoara M, Teodosiu C, Micu D, Plavan G. Toxic metals biomonitoring based on prey-predator interactions and environmental forensics techniques: A study at the Romanian-Ukraine cross border of the Black Sea. Mar Pollut Bull 2017; 124(1): 321-330. doi: 10.1016/j.marpolbul.2017.07.052
  • 32. Oros A, Pecheanu I, Mihnea R. Bioaccumulation in Mytilus galloprovincialis along the Romanian Black Sea coastal aarea. J Environ Protec Ecol 2003; 4(4): 850-56.
  • 33. Roméo M, Frasila C, Gnassia-Barelli M, Damiens G, Micu D, Mustata G. Biomonitoring of trace metals in the Black Sea (Romania) using mussels Mytilus galloprovincialis. Water Res 2005; 39(4): 596-604. doi: 10.1016/j.watres.2004.09.026
  • 34. Simeonova P, Simeonov D, Spassov L, Simeonov V. Determination and statistical interpretation of toxic metals content in mollusks and snails from Black Sea. Bulg J Chem 2013; 2(3): 105-14.
  • 35. Gorinstein S, Moncheva S, Toledo F, Arancibia-Avila P, Trakhtenberg S, Gorinstein A, Goshev I, Namiesnik J. Relationship between seawater pollution and qualitative changes in the extracted proteins from mussels Mytilus galloprovincialis. Sci Total Environ 2006; 364(1): 251-259. doi: 10.1016/j.scitotenv.2005.06.013
  • 36. Gorinstein S, Jung ST, Moncheva S, Arancibia-Avila P, Park YS, Kang SG, Goshev I, Trakhtenberg S, Namiesnik J. Partial characterization of proteins from mussel Mytilus galloprovincialis as a biomarker of contamination. Arch Environ Contam Toxicol 2005; 49(4): 504-10. doi: 10.1007/s00244-004-0238-2

Year 2019, Volume: 78 Issue: 1, 23 - 28, 31.05.2019

Levent Bat Fatih Sahin Aysah Oztekin

Abstract

References

  • 1. Bat L, Öztekin A, Şahin F, Arici E, Özsandikci U. An overview of the Black Sea pollution in Turkey. MedFAR 2018; 1(2): 67-86.
  • 2. Bat L, Özkan EY. Chapter 13. Heavy metal levels in sediment of the Turkish Black Sea coast. In: Bikarska I, Raykov V, Nikolov N. (Eds.) Progressive Engineering Practices in Marine Resource Management. IGI Global book series Advances in Environmental Engineering and Green Technologies (AEEGT) (ISSN: 2326-9162; eISSN: 2326-9170) USA, 2016; 399-419.
  • 3. Bat L, Arıcı E. Chapter 5. Heavy Metal Levels in Fish, Molluscs, and Crustacea From Turkish Seas and Potential Risk of Human Health. In: Holban AM, Grumezescu AM. (Eds.) Handbook of Food Bioengineering, Volume 13, Food Quality: Balancing Health and Disease. Elsevier, Academic Press, ISBN: 978-0-12-811442-1, 2018 ; 159-196. http://dx.doi.org/10.1016/B978-0-12-811442-1.00005-5
  • 4. Phillips DJH, Rainbow PS. Biomonitoring of trace aquatic contaminants. Environmental Management Series, Chapman & Hall, London 1994.
  • 5. Phillips DJH. The chemistries and environmental fates of trace metals and organochlorines in aquatic ecosystems. Mar Pollut Bull 1995; 31(4-12): 193-00.
  • 6. Uysal H. Türkiye sahillerinde bulunan midyeler ‘Mytilus galloprovincialis Lamark’ üzerinde biyolojik ve ekolojik araştırmalar. EÜ Fen Fak İlmi Rap Ser 1970; 79: 1-79.
  • 7. Çulha M, Bat L, Türk-Çulha S, Yeşim-Çelik M. Benthic mollusk composition of some facies in the upper-infralittoral zone of the southern Black Sea, Turkey. Turk J Zool 2010; 34: 523-32.
  • 8. Bat L, Gönlügür G. Sinop Sahillerinin üst-infralittoral zonundaki midye fasiesleri üzerinde kalitatif ve kantitatif araştırmalar. (Qualitative and quantitative investigations on mussel facies in the upper-infralittoral zone of Sinop coasts, Black Sea, Turkey). (OMÜ S.057 Numaralı Araştırma Fonu) 2001; 31 pp.
  • 9. Mülayim A, Balkis H. Toxic Metal (Pb, Cd, Cr, and Hg) levels in Rapana venosa (Valenciennes, 1846), Eriphia verrucosa (Forskal, 1775), and sediment samples from the Black Sea littoral (Thrace, Turkey). Mar Poll Bull 2015; 95: 215-22.
  • 10. Bat L, Öztekin HC. Heavy Metals in Mytilus galloprovincialis, Rapana venosa and Eriphia verrucosa from the Black Sea coasts of Turkey as bioindicators of pollution. Walailak J Sci Tech 2016; 13(9): 715-28.
  • 11. Bat L, Arici E, Öztekin A. Human health risk assessment of heavy metals in the Black Sea: evaluating mussels. Current World Environ 2018; 13(1): 15-31.
  • 12. Bat L, Arici E, Öztekin A, Yardim Ö, Üstün F. Use of the Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 from Sinop coasts of the Black Sea as bio-monitor. Int J Mar Sci 2018; 8(5): 44-7.
  • 13. Bat L, Şahin F, Öztekin A. Toxic elements in edible Mollusks from Igneada coasts of the Black Sea, Turkey. Korean J Food Health Conv 2018; 4(3): 22-31. doi: http://dx.doi.org/10.13106/kjfhc.2018.vol4.no3.22
  • 14. Bat L, Sezgin M, Şahin F, Birinci Özdemir Z, Ürkmez D. Sinop city fishery of the Black Sea. Mar Sci 2013; 3(3): 55-64. DOI: 10.5923/j. ms.20130303.01
  • 15. Bat L, Bat L, Sahin F, Sezgin M, Gonener S, Erdem E, Ozsandıkcı U. Fishery of Sinop coasts in the Black Sea surveys. Eur J Biol 2018; 77(1): 18-25. doi: 10.26650/EuroJBiol.2018.388175
  • 16. Bernhard M. Manual of methods in the aquatic environment research.Rome: Food and Agriculture Organization. 1976.
  • 17. United Nations Environment Programme. Reference methods for marine pollution studies. RSMS 11. Determination of total cadmium, zinc, lead and copper in selected marine organisms by flameless atomic absorption spectrophotometry. Geneva: United Nations Environment Programme; 1984. [Online] Available from: http://www.unep.ch/regionalseas/pubs/rsrm.htm [Accessed on 22nd November, 2016]
  • 18. United Nations Environment Programme. GESAMP: Cadmium, lead and tin the marine environment. UNEP Regional Seas Reports and Studies No. 56. Nairobi: United Nations Environment Programme; 1985. [Online] Available from: http://www.unep.org/regionalseas/ publications/reports/RSRS/pdfs/rsrs056.pdf [Accessed on 1th December, 2016]
  • 19. Zar JH. Biostatistical analysis. 2nd ed. New Jersey: Prentice Hall 1984.
  • 20. MAFF (The Ministry of Agriculture, Forestry and Fisheries). Monitoring and surveillance of non-radioactive contaminants in the aquatic environment and activities regulating the disposal wastes at sea, of 1993. Directorate of Fisheries research, Lowestoft, Aquatic Environment Monitoring Report, 1995; 44.
  • 21. TFC. Official Gazette of Republic of Turkey. Notifications about determination of the maximum levels for certain contaminants in foodstuffs of Turkish Food Codex (inTurkish). (Notification No: 2002/63) 2002; 24885.
  • 22. Council of Europe. Partial Agreement in the Social and Public Field. The Guidelines on Metals and Alloys used as Food Contact Materials are Part of the Council of Europe’s Policy Statements Concerning Materials and Articles Intented to Come into Contact with Foodstuffs. Technical Document Strasbourg, 2001.
  • 23. Commission Regulation (EC). Setting maximum levels for certain contaminants in foodstuffs. 2006; 1881.
  • 24. TFC. Official Gazette of Republic of Turkey. Notifications changes to the maximum levels for certain contaminants in foodstuffs (in Turkish). (Notification No: 2009/22) 2009; 27143.
  • 25. Beltrame MO, De Marco SG, Marcovecchio JE. Influences of sex, habitat, and seasonality on heavy-metal concentrations in the burrowing crab (Neohelice granulata) from a coastal lagoon in Argentina. Arch Environ Contam Toxicol 2010; 58(3): 746-56. doi: 10.1007/s00244-009-9405-9
  • 26. Simonetti P, Botté SE, Fiori SM, Marcovecchio JE. Heavy-metal concentrations in soft tissues of the burrowing crab Neohelice granulata in Bahía Blanca Estuary, Argentina. Arch Environ Contam Toxicol 2012; 62: 243–253. doi: 10.1007/s00244-011-9692-9
  • 27. Baltas H, Sirin M, Dalgic G, Bayrak EY, Akdeniz A. Assessment of metal concentrations (Cu, Zn, and Pb) in seawater, sediment and biota samples in the coastal area of eastern Black Sea, Turkey. Mar Pollut Bull 2017; 122(1-2): 475-82. doi: 10.1016/j.marpolbul.2017.06.059
  • 28. Demina LL, Budko DM. Trace metals in carbonate biomineralization by the example of Bivalvia Mytilus spp. from the Black Sea. (in Russian) Geologo-Mineralogical Sci Fundamental Res 2014; 11: 2185-89.
  • 29. Temerdashev ZA, Eletskii II, Kaunova AA, Korpakova IG. Determination of heavy metals in Mytilus galloprovincialis Lamarck mussels using the IСP-AES method. (in Russian). Аналитика и контроль 2017; 21(2): 116-24.
  • 30. Egorov VN, Lazorenko GE, Mirzoyeva NY, Stokozov NA, Kostova SK, Malakhova LV, Pirkova AV, Arkhipova SI, Korkishko NF, Popovichev VN, Plotitsyna OV, Migal LV. Content of 137 Cs, 40 K, 90 Sr, 210 Po radionuclides and some chemical pollutants in the Black Sea mussels, Mytilus galloprovincialis Lam. Морской экологический журнал 2006; 5(3): 70-8.
  • 31. Strungaru SA, Nicoara M, Teodosiu C, Micu D, Plavan G. Toxic metals biomonitoring based on prey-predator interactions and environmental forensics techniques: A study at the Romanian-Ukraine cross border of the Black Sea. Mar Pollut Bull 2017; 124(1): 321-330. doi: 10.1016/j.marpolbul.2017.07.052
  • 32. Oros A, Pecheanu I, Mihnea R. Bioaccumulation in Mytilus galloprovincialis along the Romanian Black Sea coastal aarea. J Environ Protec Ecol 2003; 4(4): 850-56.
  • 33. Roméo M, Frasila C, Gnassia-Barelli M, Damiens G, Micu D, Mustata G. Biomonitoring of trace metals in the Black Sea (Romania) using mussels Mytilus galloprovincialis. Water Res 2005; 39(4): 596-604. doi: 10.1016/j.watres.2004.09.026
  • 34. Simeonova P, Simeonov D, Spassov L, Simeonov V. Determination and statistical interpretation of toxic metals content in mollusks and snails from Black Sea. Bulg J Chem 2013; 2(3): 105-14.
  • 35. Gorinstein S, Moncheva S, Toledo F, Arancibia-Avila P, Trakhtenberg S, Gorinstein A, Goshev I, Namiesnik J. Relationship between seawater pollution and qualitative changes in the extracted proteins from mussels Mytilus galloprovincialis. Sci Total Environ 2006; 364(1): 251-259. doi: 10.1016/j.scitotenv.2005.06.013
  • 36. Gorinstein S, Jung ST, Moncheva S, Arancibia-Avila P, Park YS, Kang SG, Goshev I, Trakhtenberg S, Namiesnik J. Partial characterization of proteins from mussel Mytilus galloprovincialis as a biomarker of contamination. Arch Environ Contam Toxicol 2005; 49(4): 504-10. doi: 10.1007/s00244-004-0238-2
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Details

Primary Language English
Journal Section Makaleler
Authors

Levent Bat

Fatih Sahin This is me 0000-0003-0605-2672

Aysah Oztekin This is me 0000-0002-3726-7134

Publication Date May 31, 2019
Submission Date February 5, 2019
Published in Issue Year 2019 Volume: 78 Issue: 1

Cite

AMA Bat L, Sahin F, Oztekin A. Metal Bioaccumulation of Mytilaster lineatus (Gmelin, 1791) Collected From Sinop Coast in the Southern Black Sea. Eur J Biol. May 2019;78(1):23-28.
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