Research Article
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Year 2023, Volume: 7 Issue: 2, 65 - 69, 15.12.2023

Nazan Demir Hayrunnisa Nadaroğlu Yaşar Demir

Abstract

References

  • [1]Preston CD, Pearman DA, Dines TD. New Atlas of the British and Irish Flora. Oxford: Oxford University Press (2002).
  • [2]Howard M. Traditional Herbal Remedies128–129(1987).
  • [3]Rao MB, Tanksale AM, Ghatge MS, Deshpande VV. Molecular and Biotechnological Aspects of Microbial Proteases. Microbiology and Molecular Biology Reviews(1998) 62(3):597–635. doi:10.1128/MMBR.62.3.597-635.1998.
  • [4]Mamo J, Assefa F. The Role of Microbial Aspartic Protease Enzyme in Food and Beverage Industries. Journal of Food Quality(2018) 2018:1–15. doi:10.1155/2018/7957269.
  • [5]Navia MA. Three-dimensional structure of aspartyl protease from human immunodeficiency virus HIV-1. Nature(1989) 337(6208):615–620. doi:10.1038/337615a0.
  • [6]Muhammad A. Purification, Characterization and Thermodynamic Assessment of an Alkaline Protease by Geotrichum Candidum of Dairy Origin. Iranian Journal of Biotechnology(2019) 17(2):30–37. doi:10.21859/ijb.2042.
  • [7]Saranya K, Dhanachezhiyan M, Gopinath G, kumar A, babu N. STUDY OF UV MUTATED PROTEASE ENZYME FROM VARIOUS SOURCES. International Research Journal of Pharmacy(2019) 10(5):80–84. doi:10.7897/2230-8407.1005167.
  • [8]Ellatif S. ENCAPSULATION OF PROTEASE ENZYME FOR DOMESTIC APPLICATION. Al-Azhar Journal of Pharmaceutical Sciences(2020) 61(1):117–133. doi:10.21608/ajps.2020.86022.
  • [9]McGrath ME. THE LYSOSOMAL CYSTEINE PROTEASES. Annual Review of Biophysics and Biomolecular Structure(1999) 28(1):181–204. doi:10.1146/annurev.biophys.28.1.181.
  • [10]Zhang C. Stimulation of Hepatitis C Virus (HCV) Nonstructural Protein 3 (NS3) Helicase Activity by the NS3 Protease Domain and by HCV RNA-Dependent RNA Polymerase. Journal of Virology(2005) 79(14):8687–8697. doi:10.1128/JVI.79.14.8687-8697.2005.
  • [11]Li Q, Kang C. Structure and Dynamics of Zika Virus Protease and Its Insights into InhibitorDesign. Biomedicines(2021) 9(8):1044. doi:10.3390/biomedicines9081044.
  • [12]Nadaroglu H, Demir N. Purification and characterization of a novel serine protease compositain from compositae (Scorzonera hispanica L. European Food Research and Technology(2012) 234(6). doi:10.1007/s00217-012-1719-6.
  • [13]Baran A, Nadaroglu H, Önem H, Adıgüzel MC. Proteolytic activities and safety use of Enterococcus faecalis strains isolated from Turkish White Pickled Cheese and milk samples. Journal of the Hellenic Veterinary Medical Society(2022) 72(4):3391–3400. doi:10.12681/jhvms.29382.
  • [14]Demir Y, Demir N, Kaya E, Aydin B. Cysteine Protease from Primrose (Primula vulgaris. Asian Journal of Chemistry(2012) 24(4):1479–1482.
  • [15]Demİr N, Daşdemİr SN, Can Z. Purification and Characterization of Protease Enzyme from Oleander (Nerium oleander ) Flowers of Different Colors1(2017). 21–26.
  • [16]Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature(1970) 227:680.
  • [17]Demir Y, Alayli A, Yildirim S, Demir N. Identification of protease from Euphorbia amygdaloides latex and its use in cheese production. Preparative Biochemistry and Biotechnology(2005) 35(4). doi:10.1080/10826060500218107.
  • [18]Lynn KR, Clevette-Radford NA. Isolation and characterization of euphorbain 1, a proteinase from the latex of Euphorbia lathyris. Biochimica et Biophysica Acta (BBA) -Protein Structure and Molecular Enzymology(1983) 746(3):154–159. doi:10.1016/0167-4838(83)90069-9.
  • [19]Akhtaruzzaman M, Mozumder N, Jamal R, Rahman A, Rahman T. Isolation and characterization protease enzyme from leguminous seeds2(2012). 434–440.
  • [20]Amid M, Manap M, Zohdi NK. Purification and Characterization of Alkaline-Thermostable Protease Enzyme from Pitaya (Hylocereus polyrhizus) Waste: A Potential Low Cost of the Enzyme. BioMed Research International(2014) 2014. doi:10.1155/2014/259238.
  • [21]Tomar R, Kumar R, Jagannadham MV. A stable serine protease, wrightin, from the latex of the plant Wrightia tinctoria (Roxb.) R. Br.: Purification and biochemical properties. Journal of Agricultural and Food Chemistry(2008) 56(4):1479–1487. doi:10.1021/jf0726536.
  • [22]Dubey V, Jagannadham M. Procerain, a stable cysteine protease from the latex of Calotropis procera. Phytochemistry(2003) 62(7):1057–1071. doi:10.1016/S0031-9422(02)00676-3.
  • [23]Anigboro AA, Avwioroko OJ, Ohwokevwo OA, Pessu B. Bioactive components of Ficus exasperata, Moringa oleifera and Jatropha tanjorensis leaf extracts and evaluation of their antioxidant properties -ProQuest. EurAsian Journal of BioSciences(2019) 13:1763–1769.

Purification of Protease Enzyme from Freesia (Freesia refracta) Flowers and Investigation of Its Use in Cosmetics

Year 2023, Volume: 7 Issue: 2, 65 - 69, 15.12.2023

Nazan Demir Hayrunnisa Nadaroğlu Yaşar Demir

Abstract

This study aims to research and analyze a newly identified plant-based protease for potential applications within the cosmetic industry. The research focuses on isolating and characterizing a protease enzyme derived from Freesia refracta flowers. The purification method involves employing ammonium sulfate precipitation and ion exchange chromatography. Additionally, the protease's substrate specificity will beexamined, particularly its reactions with azoalbumin and serum albumin.Enzymatic kinetics, inclusive of determining Vmax and Km values using Lineweaver-Burk plots, yielded readings of 0.784 mg/L.min and 0.317 mM, respectively. Furthermore, SDS-PAGE and gel filtration chromatography were utilized to determine the enzyme's degree of purification and molecular weight, indicating a molecular weight of 24 kDa. These outcomes identify the specific protease obtained from Freesia flowers, suggesting its potential suitability for cosmetic applications.Moreover, investigations into the impact of various compounds SDS, DIPF, EDTA, phenanthraline, iodoacetamide, Ca2+, Zn2+, Hg2+, and Co2+at a 10 mM concentration significantly enhanced the enzymatic activity of the purified protease. The research findings affirm the successful utilization of the protease extracted from Freesia flowers (Freesia refracta) in cosmetic formulations, showcasing its practical suitability for cosmetic production.

Keywords

Protease Frezya(Freesia refracta) Casain Cosmetic productions

References

  • [1]Preston CD, Pearman DA, Dines TD. New Atlas of the British and Irish Flora. Oxford: Oxford University Press (2002).
  • [2]Howard M. Traditional Herbal Remedies128–129(1987).
  • [3]Rao MB, Tanksale AM, Ghatge MS, Deshpande VV. Molecular and Biotechnological Aspects of Microbial Proteases. Microbiology and Molecular Biology Reviews(1998) 62(3):597–635. doi:10.1128/MMBR.62.3.597-635.1998.
  • [4]Mamo J, Assefa F. The Role of Microbial Aspartic Protease Enzyme in Food and Beverage Industries. Journal of Food Quality(2018) 2018:1–15. doi:10.1155/2018/7957269.
  • [5]Navia MA. Three-dimensional structure of aspartyl protease from human immunodeficiency virus HIV-1. Nature(1989) 337(6208):615–620. doi:10.1038/337615a0.
  • [6]Muhammad A. Purification, Characterization and Thermodynamic Assessment of an Alkaline Protease by Geotrichum Candidum of Dairy Origin. Iranian Journal of Biotechnology(2019) 17(2):30–37. doi:10.21859/ijb.2042.
  • [7]Saranya K, Dhanachezhiyan M, Gopinath G, kumar A, babu N. STUDY OF UV MUTATED PROTEASE ENZYME FROM VARIOUS SOURCES. International Research Journal of Pharmacy(2019) 10(5):80–84. doi:10.7897/2230-8407.1005167.
  • [8]Ellatif S. ENCAPSULATION OF PROTEASE ENZYME FOR DOMESTIC APPLICATION. Al-Azhar Journal of Pharmaceutical Sciences(2020) 61(1):117–133. doi:10.21608/ajps.2020.86022.
  • [9]McGrath ME. THE LYSOSOMAL CYSTEINE PROTEASES. Annual Review of Biophysics and Biomolecular Structure(1999) 28(1):181–204. doi:10.1146/annurev.biophys.28.1.181.
  • [10]Zhang C. Stimulation of Hepatitis C Virus (HCV) Nonstructural Protein 3 (NS3) Helicase Activity by the NS3 Protease Domain and by HCV RNA-Dependent RNA Polymerase. Journal of Virology(2005) 79(14):8687–8697. doi:10.1128/JVI.79.14.8687-8697.2005.
  • [11]Li Q, Kang C. Structure and Dynamics of Zika Virus Protease and Its Insights into InhibitorDesign. Biomedicines(2021) 9(8):1044. doi:10.3390/biomedicines9081044.
  • [12]Nadaroglu H, Demir N. Purification and characterization of a novel serine protease compositain from compositae (Scorzonera hispanica L. European Food Research and Technology(2012) 234(6). doi:10.1007/s00217-012-1719-6.
  • [13]Baran A, Nadaroglu H, Önem H, Adıgüzel MC. Proteolytic activities and safety use of Enterococcus faecalis strains isolated from Turkish White Pickled Cheese and milk samples. Journal of the Hellenic Veterinary Medical Society(2022) 72(4):3391–3400. doi:10.12681/jhvms.29382.
  • [14]Demir Y, Demir N, Kaya E, Aydin B. Cysteine Protease from Primrose (Primula vulgaris. Asian Journal of Chemistry(2012) 24(4):1479–1482.
  • [15]Demİr N, Daşdemİr SN, Can Z. Purification and Characterization of Protease Enzyme from Oleander (Nerium oleander ) Flowers of Different Colors1(2017). 21–26.
  • [16]Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature(1970) 227:680.
  • [17]Demir Y, Alayli A, Yildirim S, Demir N. Identification of protease from Euphorbia amygdaloides latex and its use in cheese production. Preparative Biochemistry and Biotechnology(2005) 35(4). doi:10.1080/10826060500218107.
  • [18]Lynn KR, Clevette-Radford NA. Isolation and characterization of euphorbain 1, a proteinase from the latex of Euphorbia lathyris. Biochimica et Biophysica Acta (BBA) -Protein Structure and Molecular Enzymology(1983) 746(3):154–159. doi:10.1016/0167-4838(83)90069-9.
  • [19]Akhtaruzzaman M, Mozumder N, Jamal R, Rahman A, Rahman T. Isolation and characterization protease enzyme from leguminous seeds2(2012). 434–440.
  • [20]Amid M, Manap M, Zohdi NK. Purification and Characterization of Alkaline-Thermostable Protease Enzyme from Pitaya (Hylocereus polyrhizus) Waste: A Potential Low Cost of the Enzyme. BioMed Research International(2014) 2014. doi:10.1155/2014/259238.
  • [21]Tomar R, Kumar R, Jagannadham MV. A stable serine protease, wrightin, from the latex of the plant Wrightia tinctoria (Roxb.) R. Br.: Purification and biochemical properties. Journal of Agricultural and Food Chemistry(2008) 56(4):1479–1487. doi:10.1021/jf0726536.
  • [22]Dubey V, Jagannadham M. Procerain, a stable cysteine protease from the latex of Calotropis procera. Phytochemistry(2003) 62(7):1057–1071. doi:10.1016/S0031-9422(02)00676-3.
  • [23]Anigboro AA, Avwioroko OJ, Ohwokevwo OA, Pessu B. Bioactive components of Ficus exasperata, Moringa oleifera and Jatropha tanjorensis leaf extracts and evaluation of their antioxidant properties -ProQuest. EurAsian Journal of BioSciences(2019) 13:1763–1769.
There are 23 citations in total.

Details

Primary Language English
Subjects Medical Biotechnology (Other), Micro and Nanosystems
Journal Section Research Articles
Authors

Nazan Demir

Hayrunnisa Nadaroğlu

Yaşar Demir

Publication Date December 15, 2023
Submission Date October 10, 2023
Acceptance Date December 10, 2023
Published in Issue Year 2023 Volume: 7 Issue: 2

Cite

APA Demir, N., Nadaroğlu, H., & Demir, Y. (2023). Purification of Protease Enzyme from Freesia (Freesia refracta) Flowers and Investigation of Its Use in Cosmetics. International Journal of Innovative Research and Reviews, 7(2), 65-69.
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