Research Article
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Antiproliferative Effect of IST-GLIO® Supplement Food Product on C6 Glioma Cells

Year 2020, Volume: 1 Issue: 2, 42 - 47, 27.08.2020

Abstract

Objective: Cancer is one of the leading causes of death worldwide and is characterized by the proliferation of abnormal cells. In recent years, as in the treatment of many diseases, there is also a return to nature in the treatment of cancer. In developing societies, the concepts such as prophylaxis of disease prevention rise to prominence in order to reduce the material and moral losses caused by disease treatment. Consequently, the use of supplementary food products occupies more and more space in daily life. In this study, it was aimed to investigate the antiproliferative properties of IST-GLIO® food supplement, which is one of these products and used in the Remember Regeneration Therapy Method (RTM), on C6 (rat glioma) cells.
Material-Method: WST-1 cell proliferation test protocol was applied to examine the antiproliferative effect of IST-GLIO® and the results were evaluated according to ELISA microplate reading data. The product was tested at concentrations ranging from 1 ng/mL to 100 ng/mL.
Results: When the results were evaluated, it was appeared to be effective in the concentration range of 6-20 ng/mL on the C6 glioma cell culture; it was found that the efficacy didn’t occur through general toxicity, specifically reducing the reproduction of the cancer cells in question, since food supplement did not inhibit cell division further at higher doses.
Conclusion: As a result, non-specific toxicity is observed in many cultures that are similarly investigated in cell cultures, depending on concentration as high doses are obtained. The IST-GLIO® product does not show this feature is considered as promising.

References

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  • Ranjbari J, Alibakhshi A, Arezumand R. Pourhassan MM, Rahmatı M, Zarghamı N, Namvaran MM. Effects of Curcuma longa extract on telomerase activity in lung and breast cancer cells. Zahedan Journal of Research in Medical Sciences. 2014;16(10):1-6.
  • Tomeh MA, Hadianamrei R, Zhao X. A review of curcumin and its derivatives as anticancer agents. International Journal of Molecular Sciences. 2019;20(5).
  • Groshi AA, Evans AR, Ismail FMD, Nahar L. Sarker SD. Cytotoxicity of Libyan Juniperus phoenicea against human cancer cell lines A549, EJ138, Hepg2 and MCF7. Pharmaceutical Sciences 2018;24(1):3-7.
  • Abaza MS, Orabi KY, Al-Quattan E, Al-Attiyah RJ. Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer. Cancer Cell Int. 2015;24:15.
  • El-Garawani IM, El-Nabi SH, Dawoud GT, Esmail SM, Abdel Moneim AE. Triggering of apoptosis and cell cycle arrest by fennel and clove oils in Caco-2 cells: the role of combination. Toxicology Mechanisms and Methods. 2019;29(9):710-722.
  • Yasar M, Senogul O, Gok A, Agan K, Agan AF, Beyazcicek E, Coskun SK. Systemic Investigation of Acute Toxicity of Some Food Supplements on the Market in Turkey. International Journal of Traditional and Complementary Medicine Research. 2020(1):1:1-11.
Year 2020, Volume: 1 Issue: 2, 42 - 47, 27.08.2020

Abstract

References

  • Nussbaum LR, McInnes RR, Willard FH. Genetik ve Kanser. Thompson&Thompson Genetics in Medicine. İstanbul, Güneş Kitabevi, 2005.
  • Derici E. Rho kinaz ve siklooksijenaz inhibitörleri ile siklofosfamid kombinasyonlarının çeşitli tümör hücre serilerine etkileri. Mersin Üniversitesi Sağlık Bilimleri Enstitüsü, Doktora Tezi. 2007.
  • Mikkelsen T. Cytostatic agents in the management of malignant gliomas. Cancer Control:JMCC 1998; 5(2):150-162.
  • Salford LG, Siesjö P, Skagerberg G, Persson BRR, Larsson EM, Lindvall M, Visse E, Widegren B. Search for effective therapy against glioblastoma multiforme clinical immunisation with autologous glioma cells transduced with the human interferon- γ gene. International Congress Series. 2002; 1247:211-220.
  • Hsu CL, Huang SL, Yen GC. Inhibitory effect of phenolic acids on the proliferation of 3T3-L1 preadipocytes in relation to their antioxidant activity. Journal of Agricultural and Food Chemistry. 2006;54(12): 4191-4197.
  • Granado-Serrano AB, Martın MA, Izquıerdo-Pulıdo M, Goya L, Bravo L, Ramos S. Molecular mechanisms of (-)-Epicatechin and Chlorogenic acid on the regulation of the apoptotic and survival/proliferation pathways in a human hepatoma cell line. Journal of Agricultural and Food Chemistry. 2007;55(5):2020-2027.
  • Marinova EM, Toneva A, Yanishlieva N. Comparison of the antioxidative properties of caffeic and chlorogenic acids. Food Chemistry. 2009;114(4): 1498–1502.
  • Vizotto M, Porter W, Byrne D, Cisneros-Zevallos L. Polyphenols of selected peach and plum genotypes reduce cell viability and inhibit proliferation of breast cancer cells while not affecting normal cells. Food Chemistry. 2014;1:64: 363–370.
  • Uçar T. Bazı bitkisel çayların mineral madde içeriği üzerine farklı demleme ve kaynatma sürelerinin etkisi. Konya, Selçuk Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi. 2006.
  • Yasar M. The remember regeneration therapy method: An overview of new therapy protocol to approach diseases. Journal of Complementary Medicine Research. 2019;10(1):68-80.
  • Doyle A, Griffiths JB. Cell and tissue culture: Laboratory procedures in biotechnology. Scientific Consultancy & Publishing. 1998;62-64.
  • Alawode TT. An overview of the anti-cancer properties of some plants used in traditional medicine in Nigeria. International Research Journal of Biochemistry and Bioinformatics. 2013;3(1):7-14.
  • Mohammadi A, Mansoori B, Aghapour M, Shirjang S, Nami S, Baradaran B. The Urtica dioica extract enhances sensitivity of paclitaxel drug to MDA-MB-468 breast cancer cells. Biomedicine Pharmacotherapy. 2016; 83:835-842.
  • Durak I, Biri H, Devrim E, Sözen S, Avcı A. Aqueous extract of Urtica dioica makes significant inhibition on adenosine deaminase activity in prostate tissue from patients with prostate cancer. Cancer Biology Therapy. 2004;3(9):855–857.
  • Konrad L, Müller HH, Lenz C, Laubinger H, Aumüller G, Lichius JJ. Antiproliferative effect on human prostate cancer cells by a stinging nettle root (Urtica dioica) extract. Planta Medicine. 2000;66(1):44–47.
  • Shabani SHS, Tehrani SSH, Rabiei Z, Enferadi ST, Vanoozzi GP. Peganum harmala L.’s anti growth effect on a breast cancer cell line. Biotechnology Reports. 2015;(8):138-143.
  • Abascal K, Yarnell E. The many faces of Silybum marianum (milk thistle). Alternative and Complementary Therapies. 2003;9(5):251–256.
  • Moheghi N, Tavakkol Afshari J, Brook A. The cytotoxic effect of Zingiber officinale in breast cancer (MCF7) cell line. The Horizon of Medical Sciences. 2011;17(3):28–34.
  • Khurshid Y, Syed B, Simjee SU, Beg O, Ahmed A. Antiproliferative and apoptotic effects of proteins from black seeds (Nigella sativa) on human breast MCF-7 cancer cell line. Complementary Medicine and Therapies. 2020;13;20(1):5.
  • Ait Mbarek L, Ait Mouse H, Elabbadi N, Bensalah M, Gamouh A, Aboufatima R, Benharref A, Chait A, Kamal M, Dalal A, Zyad A. Anti-tumor properties of blackseed (Nigella sativa L.) extracts. Brazilian Journal of Medical and Biological Research. 2007;40:839–847.
  • Ranjbari J, Alibakhshi A, Arezumand R. Pourhassan MM, Rahmatı M, Zarghamı N, Namvaran MM. Effects of Curcuma longa extract on telomerase activity in lung and breast cancer cells. Zahedan Journal of Research in Medical Sciences. 2014;16(10):1-6.
  • Tomeh MA, Hadianamrei R, Zhao X. A review of curcumin and its derivatives as anticancer agents. International Journal of Molecular Sciences. 2019;20(5).
  • Groshi AA, Evans AR, Ismail FMD, Nahar L. Sarker SD. Cytotoxicity of Libyan Juniperus phoenicea against human cancer cell lines A549, EJ138, Hepg2 and MCF7. Pharmaceutical Sciences 2018;24(1):3-7.
  • Abaza MS, Orabi KY, Al-Quattan E, Al-Attiyah RJ. Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer. Cancer Cell Int. 2015;24:15.
  • El-Garawani IM, El-Nabi SH, Dawoud GT, Esmail SM, Abdel Moneim AE. Triggering of apoptosis and cell cycle arrest by fennel and clove oils in Caco-2 cells: the role of combination. Toxicology Mechanisms and Methods. 2019;29(9):710-722.
  • Yasar M, Senogul O, Gok A, Agan K, Agan AF, Beyazcicek E, Coskun SK. Systemic Investigation of Acute Toxicity of Some Food Supplements on the Market in Turkey. International Journal of Traditional and Complementary Medicine Research. 2020(1):1:1-11.
There are 26 citations in total.

Details

Primary Language English
Subjects Traditional, Complementary and Integrative Medicine
Journal Section Research Articles
Authors

Mustafa Yaşar 0000-0002-3746-5042

Güliz Saraç 0000-0002-5832-7597

Pınar Ağyar Yoldaş 0000-0002-9277-0648

Aydan Fülden Ağan 0000-0002-4319-6264

Ertuğrul Kaya 0000-0003-4103-9537

Publication Date August 27, 2020
Submission Date May 29, 2020
Published in Issue Year 2020 Volume: 1 Issue: 2

Cite

APA Yaşar, M., Saraç, G., Ağyar Yoldaş, P., Ağan, A. F., et al. (2020). Antiproliferative Effect of IST-GLIO® Supplement Food Product on C6 Glioma Cells. International Journal of Traditional and Complementary Medicine Research, 1(2), 42-47.

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