Research Article
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Year 2020, Volume: 1 Issue: 1, 18 - 24, 30.04.2020

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References

  • 1. Li T, Jiang S, Yang Y. Database selection and heterogeneity?more details, more credibility. JAMA Oncol. 2018;4(9):1295. doi:10.1001/jamaoncol.2018.1209 2. Picci P, Mercuri M, Ferrari S, et al. Survival in high-grade osteosarcoma: Improvement over 21 years at a single institution. Ann Oncol. 2009;21(6):1366-1373. doi:10.1093/annonc/mdp502 3. Mertens WC, Bramwell V. Osteosarcoma and other tumors of bone. Curr Opin Oncol. 1994;6(4):384-390. doi:10.1097/00001622-199407000-00010 4. Mirabello L, Troisi RJ, Savage SA. Osteosarcoma incidence and survival rates from 1973 to 2004. Cancer. 2009;115(7):1531-1543. doi:10.1002/cncr.24121 5. Winslow LC, David K j. Herbs as Medicines. 2015;158. 6. Ma XH, Zheng CJ, Han LY, et al. Synergistic therapeutic actions of herbal ingredients and their mechanisms from molecular interaction and network perspectives. Drug Discov Today. 2009;14(11-12):579-588. doi:10.1016/j.drudis.2009.03.012 7. Xiangyong Y, Zhongsheng Y, Wenchao L, et al. External application of traditional Chinese medicine in the treatment of bone cancer pain: a meta-analysis. Support Care Cancer. 2016;24(1):11-17. doi:10.1007/s00520-015-2737-2 8. Atalay D, Erge HS. Dietary Supplements and Their Effects on Health. Food Heal. 2018;4(2):98-111. doi:10.3153/fh18010 9. Lange D. Europe’s medicinal and aromatic plants: their use, trade and conservation. Eur Med Aromat plants their use, trade Conserv. 1998. https://www.cabdirect.org/cabdirect/abstract/19980314129. Accessed October 2, 2018. 10. Manel M, Nouzha H, Rim M, et al. Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria. Vet World. 2018;11(3):373-378. doi:10.14202/vetworld.2018.373-378 11. Kusari S, Lamshöft M, Spiteller M. Aspergillus fumigatus Fresenius, an endophytic fungus from Juniperus communis L. Horstmann as a novel source of the anticancer pro-drug deoxypodophyllotoxin. J Appl Microbiol. 2009;107(3):1019-1030. doi:10.1111/j.1365-2672.2009.04285.x 12. Fernandez A, Journal IC-P, 2016 undefined. The Therapeutic Properties of Juniperus communis L.: Antioxidant Capacity, Bacterial growth Inhibition, Anticancer Activity and Toxicity. PhcogjCom. https://sci-hub.tw/http://phcogj.com/article/148. 13. Lim GP, Chu T, Yang F, Beech W, Frautschy S a, Cole GM. The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse. J Neurosci. 2001;21(21):8370-8377. doi:21/21/8370 [pii] 14. Sharma RA, Mclelland HR, Hill KA, et al. Pharmacodynamic and Pharmacokinetic Study of Oral Curcuma Extract in Patients with Colorectal Cancer Pharmacodynamic and Pharmacokinetic Study of Oral Curcuma Extract in Patients with Colorectal Cancer 1. 2001;7(July):1894-1900. 15. Jurenka JS, Ascp MT. Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research. Altern Med Rev. 2009;14(2):141-153. http://www.ncbi.nlm.nih.gov/pubmed/19594223. 16. Palla AH, Khan NA, Bashir S, ur-Rehman N, Iqbal J, Gilani A-H. Pharmacological basis for the medicinal use of Linum usitatissimum (Flaxseed) in infectious and non-infectious diarrhea. J Ethnopharmacol. 2015;160:61-68. doi:10.1016/J.JEP.2014.11.030 17. Moore J, Yousef M, Tsiani E. Anticancer Effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary Extract Polyphenols. 2016. doi:10.3390/nu8110731 18. Daoud A, Song J, Xiao F, Shang J. B-9-3, a novel β-carboline derivative exhibits anti-cancer activity via induction of apoptosis and inhibition of cell migration in vitro. Eur J Pharmacol. 2014;724(1):219-230. doi:10.1016/j.ejphar.2013.12.038 19. Gao J, Zhu H, Wan H, Zou X, Ma X, Gao G. Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway. Oncol Rep. 2017;38(5):2927-2934. doi:10.3892/or.2017.5952 20. Hashemi Sheikh Shabani S, Seyed Hasan Tehrani S, Rabiei Z, Tahmasebi Enferadi S, Vannozzi GP. Peganum harmala L.’s anti-growth effect on a breast cancer cell line. Biotechnol Reports. 2015;8:138-143. doi:10.1016/j.btre.2015.08.007 21. Yasar M. The remember regeneration therapy method: An overview of new therapy protocol to approach diseases. J Complement Med Res. 2019;10(1):68. doi:10.5455/jcmr.20181229122909 22. Liu B, Liu J, Chen J, Zhu D, Zhou H, Wang X. A study on anticancer activity of Caulis Spatholobi extract on human osteosarcoma Saos-2 cells. Afr J Tradit Complement Altern Med. 2013;10(5):256-260. doi:10.4314/ajtcam.v10i5.6 23. Engel N, Ali I, Adamus A, et al. Antitumor evaluation of two selected Pakistani plant extracts on human bone and breast cancer cell lines. BMC Complement Altern Med. 2016;16(1):1-18. doi:10.1186/s12906-016-1215-9 24. Chen X, Gu N, Xue C, Li BR. Plant flavonoid taxifolin inhibits the growth, migration and invasion of human osteosarcoma cells. Mol Med Rep. 2018;17(2):3239-3245. doi:10.3892/mmr.2017.8271 25. Zarringhalami R, Hanachi P, Kaya E, Ağan AF, Ağan K, Donmez M. Investigation of total phenolic content of tilia dasystyla and polygonatum orientale desf extracts and their cytotoxic effect on the osteogenic sarcoma (Saos-2) cancer cell line. Int J Cancer Manag. 2020;13(2). doi:10.5812/ijcm.94130 26. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63. doi:10.1016/0022-1759(83)90303-4 27. Lima FT, Seba V, Silva G, et al. The curcumin analog CH-5 exerts anticancer effects in human osteosarcoma cells via modulation of transcription factors p53/Sp1. Int J Mol Sci. 2018;19(7). doi:10.3390/ijms19071909 28. Zhang Y, Ma R, Cheng S, Gu G. Marrubenol inhibits osteosarcoma cancer cell growth by inducing autophagic cell death and inhibiting cancer cell migration and invasion. J BUON. 2018;23(3):729-734. 29. Mohammad P, Nosratollah Z, Mohammad R, Abbas A, Javad R. The inhibitory effect of Curcuma longa extract on telomerase activity in A549 lung cancer cell line. African J Biotechnol. 2010;9(6):912-919. 30. Ranjbari J, Alibakhshi A, Arezumand R, et al. Effects of Curcuma longa Extract on Telomerase Activity in Lung and Breast Cancer Cells Javad. Zahedan J Res Med Sci. 2013;16(10):1-6. 31. Moore J, Yousef M, Tsiani E. Anticancer Effects of Rosemary (Rosmarinus Officinalis L.) Extract and Rosemary Extract Polyphenols. Vol 8.; 2016. doi:10.3390/nu8110731 32. Mohammadi A, Mansoori B, Aghapour M, Shirjang S, Nami S, Baradaran B. The Urtica dioica extract enhances sensitivity of paclitaxel drug to MDA-MB-468 breast cancer cells. Biomed Pharmacother. 2016;83:835-842. doi:10.1016/j.biopha.2016.07.056 33. Mohammadi A, Mansoori B, Aghapour M, Baradaran B. Urtica dioica dichloromethane extract induce apoptosis from intrinsic pathway on human prostate cancer cells (PC3). Cell Mol Biol. 2016;62(3):78-83. doi:10.14715/cmb/2016.62.3.13 34. Groshi A Al, Evans AR, Ismail FMD, Nahar L, Sarker SD. Cytotoxicity of libyan juniperus phoenicea against human cancer cell lines A549, EJ138, Hepg2 and MCF7. Pharm Sci. 2018;24(1):3-7. doi:10.15171/PS.2018.02 35. Van Slambrouck S, Daniels AL, Hooten CJ, et al. Effects of crude aqueous medicinal plant extracts on growth and invasion of breast cancer cells. Oncol Rep. 2007;17(6):1487-1492. doi:10.3892/or.17.6.1487 36. Vemuri SK, Banala RR, Subbaiah GPV, Srivastava SK, Reddy AVG, Malarvili T. Anti-cancer potential of a mix of natural extracts of turmeric, ginger and garlic: A cell-based study. Egypt J Basic Appl Sci. 2017;4(4):332-344. doi:10.1016/j.ejbas.2017.07.005 37. Hashemi Sheikh Shabani S, Seyed Hasan Tehrani S, Rabiei Z, Tahmasebi Enferadi S, Vannozzi GP. Peganum harmala L.’s anti-growth effect on a breast cancer cell line. Biotechnol Reports. 2015;8:138-143. doi:10.1016/j.btre.2015.08.007 38. Zhang Y, Xie WP, Zhang YK, et al. Experimental study of inhibitory effects of diallyl trisulfide on the growth of human osteosarcoma saos-2 cells by downregulating expression of glucose-regulated protein 78. Onco Targets Ther. 2018;11:271-277. doi:10.2147/OTT.S150933 39. Luo Z, Li D, Luo X, et al. Curcumin may serve an anticancer role in human osteosarcoma cell line U-2 OS by targeting ITPR1. Oncol Lett. 2018;15(4):5593-5601. doi:10.3892/ol.2018.8032 40. Walters DK, Muff R, Langsam B, Born W, Fuchs B. Cytotoxic effects of curcumin on osteosarcoma cell lines. Invest New Drugs. 2008;26(4):289-297. doi:10.1007/s10637-007-9099-7

Anticancer Effect of Food Supplements on Saos-2 Osteosarcoma Cell

Year 2020, Volume: 1 Issue: 1, 18 - 24, 30.04.2020

Abstract

Objective: Nowadays medicinal plants have been considered as complementary medicine in cancer treatment by many researchers. Osteosarcoma, one of the most common malignant tumors, which is more common at a young age; It is one of the most common types of cancer that occur in mesenchymal bone-forming cells and affect the musculoskeletal system. Today instead of cancer drugs due to their numerous side effects that can suppress or prevent cancer treatment, food supplement products that contain plant extracts may be preferred. In this study, the anticancer effects of five different food supplements were investigated on osteosarcoma Saos-2 cancer cell line. 
Material-Method: Application doses were as 1000, 2000, 3000, 4000, 5000, 6000 µg/ml and IC50 values were calculated by creating two working groups of 24 and 48 hours. 
Results: Extracts of five different herbal product showed cytotoxic effect on Saos-2 cancer cell line due to their mixture of medicinal plants, however REGULIN® showed the most cytotoxic effect among them.
Conclusion: It was determined that REGULIN® used in the remember regeneration therapy method (RTM), had the most anticancer effect in each of the 24 and 48 hour application groups. Cytotoxic effect of these food products on other cancer cell lines and their use in the pharmaceutical and health industries is recommended in future studies.

References

  • 1. Li T, Jiang S, Yang Y. Database selection and heterogeneity?more details, more credibility. JAMA Oncol. 2018;4(9):1295. doi:10.1001/jamaoncol.2018.1209 2. Picci P, Mercuri M, Ferrari S, et al. Survival in high-grade osteosarcoma: Improvement over 21 years at a single institution. Ann Oncol. 2009;21(6):1366-1373. doi:10.1093/annonc/mdp502 3. Mertens WC, Bramwell V. Osteosarcoma and other tumors of bone. Curr Opin Oncol. 1994;6(4):384-390. doi:10.1097/00001622-199407000-00010 4. Mirabello L, Troisi RJ, Savage SA. Osteosarcoma incidence and survival rates from 1973 to 2004. Cancer. 2009;115(7):1531-1543. doi:10.1002/cncr.24121 5. Winslow LC, David K j. Herbs as Medicines. 2015;158. 6. Ma XH, Zheng CJ, Han LY, et al. Synergistic therapeutic actions of herbal ingredients and their mechanisms from molecular interaction and network perspectives. Drug Discov Today. 2009;14(11-12):579-588. doi:10.1016/j.drudis.2009.03.012 7. Xiangyong Y, Zhongsheng Y, Wenchao L, et al. External application of traditional Chinese medicine in the treatment of bone cancer pain: a meta-analysis. Support Care Cancer. 2016;24(1):11-17. doi:10.1007/s00520-015-2737-2 8. Atalay D, Erge HS. Dietary Supplements and Their Effects on Health. Food Heal. 2018;4(2):98-111. doi:10.3153/fh18010 9. Lange D. Europe’s medicinal and aromatic plants: their use, trade and conservation. Eur Med Aromat plants their use, trade Conserv. 1998. https://www.cabdirect.org/cabdirect/abstract/19980314129. Accessed October 2, 2018. 10. Manel M, Nouzha H, Rim M, et al. Antibacterial and antioxidant activity of Juniperus thurifera L. leaf extracts growing in East of Algeria. Vet World. 2018;11(3):373-378. doi:10.14202/vetworld.2018.373-378 11. Kusari S, Lamshöft M, Spiteller M. Aspergillus fumigatus Fresenius, an endophytic fungus from Juniperus communis L. Horstmann as a novel source of the anticancer pro-drug deoxypodophyllotoxin. J Appl Microbiol. 2009;107(3):1019-1030. doi:10.1111/j.1365-2672.2009.04285.x 12. Fernandez A, Journal IC-P, 2016 undefined. The Therapeutic Properties of Juniperus communis L.: Antioxidant Capacity, Bacterial growth Inhibition, Anticancer Activity and Toxicity. PhcogjCom. https://sci-hub.tw/http://phcogj.com/article/148. 13. Lim GP, Chu T, Yang F, Beech W, Frautschy S a, Cole GM. The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse. J Neurosci. 2001;21(21):8370-8377. doi:21/21/8370 [pii] 14. Sharma RA, Mclelland HR, Hill KA, et al. Pharmacodynamic and Pharmacokinetic Study of Oral Curcuma Extract in Patients with Colorectal Cancer Pharmacodynamic and Pharmacokinetic Study of Oral Curcuma Extract in Patients with Colorectal Cancer 1. 2001;7(July):1894-1900. 15. Jurenka JS, Ascp MT. Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research. Altern Med Rev. 2009;14(2):141-153. http://www.ncbi.nlm.nih.gov/pubmed/19594223. 16. Palla AH, Khan NA, Bashir S, ur-Rehman N, Iqbal J, Gilani A-H. Pharmacological basis for the medicinal use of Linum usitatissimum (Flaxseed) in infectious and non-infectious diarrhea. J Ethnopharmacol. 2015;160:61-68. doi:10.1016/J.JEP.2014.11.030 17. Moore J, Yousef M, Tsiani E. Anticancer Effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary Extract Polyphenols. 2016. doi:10.3390/nu8110731 18. Daoud A, Song J, Xiao F, Shang J. B-9-3, a novel β-carboline derivative exhibits anti-cancer activity via induction of apoptosis and inhibition of cell migration in vitro. Eur J Pharmacol. 2014;724(1):219-230. doi:10.1016/j.ejphar.2013.12.038 19. Gao J, Zhu H, Wan H, Zou X, Ma X, Gao G. Harmine suppresses the proliferation and migration of human ovarian cancer cells through inhibiting ERK/CREB pathway. Oncol Rep. 2017;38(5):2927-2934. doi:10.3892/or.2017.5952 20. Hashemi Sheikh Shabani S, Seyed Hasan Tehrani S, Rabiei Z, Tahmasebi Enferadi S, Vannozzi GP. Peganum harmala L.’s anti-growth effect on a breast cancer cell line. Biotechnol Reports. 2015;8:138-143. doi:10.1016/j.btre.2015.08.007 21. Yasar M. The remember regeneration therapy method: An overview of new therapy protocol to approach diseases. J Complement Med Res. 2019;10(1):68. doi:10.5455/jcmr.20181229122909 22. Liu B, Liu J, Chen J, Zhu D, Zhou H, Wang X. A study on anticancer activity of Caulis Spatholobi extract on human osteosarcoma Saos-2 cells. Afr J Tradit Complement Altern Med. 2013;10(5):256-260. doi:10.4314/ajtcam.v10i5.6 23. Engel N, Ali I, Adamus A, et al. Antitumor evaluation of two selected Pakistani plant extracts on human bone and breast cancer cell lines. BMC Complement Altern Med. 2016;16(1):1-18. doi:10.1186/s12906-016-1215-9 24. Chen X, Gu N, Xue C, Li BR. Plant flavonoid taxifolin inhibits the growth, migration and invasion of human osteosarcoma cells. Mol Med Rep. 2018;17(2):3239-3245. doi:10.3892/mmr.2017.8271 25. Zarringhalami R, Hanachi P, Kaya E, Ağan AF, Ağan K, Donmez M. Investigation of total phenolic content of tilia dasystyla and polygonatum orientale desf extracts and their cytotoxic effect on the osteogenic sarcoma (Saos-2) cancer cell line. Int J Cancer Manag. 2020;13(2). doi:10.5812/ijcm.94130 26. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63. doi:10.1016/0022-1759(83)90303-4 27. Lima FT, Seba V, Silva G, et al. The curcumin analog CH-5 exerts anticancer effects in human osteosarcoma cells via modulation of transcription factors p53/Sp1. Int J Mol Sci. 2018;19(7). doi:10.3390/ijms19071909 28. Zhang Y, Ma R, Cheng S, Gu G. Marrubenol inhibits osteosarcoma cancer cell growth by inducing autophagic cell death and inhibiting cancer cell migration and invasion. J BUON. 2018;23(3):729-734. 29. Mohammad P, Nosratollah Z, Mohammad R, Abbas A, Javad R. The inhibitory effect of Curcuma longa extract on telomerase activity in A549 lung cancer cell line. African J Biotechnol. 2010;9(6):912-919. 30. Ranjbari J, Alibakhshi A, Arezumand R, et al. Effects of Curcuma longa Extract on Telomerase Activity in Lung and Breast Cancer Cells Javad. Zahedan J Res Med Sci. 2013;16(10):1-6. 31. Moore J, Yousef M, Tsiani E. Anticancer Effects of Rosemary (Rosmarinus Officinalis L.) Extract and Rosemary Extract Polyphenols. Vol 8.; 2016. doi:10.3390/nu8110731 32. Mohammadi A, Mansoori B, Aghapour M, Shirjang S, Nami S, Baradaran B. The Urtica dioica extract enhances sensitivity of paclitaxel drug to MDA-MB-468 breast cancer cells. Biomed Pharmacother. 2016;83:835-842. doi:10.1016/j.biopha.2016.07.056 33. Mohammadi A, Mansoori B, Aghapour M, Baradaran B. Urtica dioica dichloromethane extract induce apoptosis from intrinsic pathway on human prostate cancer cells (PC3). Cell Mol Biol. 2016;62(3):78-83. doi:10.14715/cmb/2016.62.3.13 34. Groshi A Al, Evans AR, Ismail FMD, Nahar L, Sarker SD. Cytotoxicity of libyan juniperus phoenicea against human cancer cell lines A549, EJ138, Hepg2 and MCF7. Pharm Sci. 2018;24(1):3-7. doi:10.15171/PS.2018.02 35. Van Slambrouck S, Daniels AL, Hooten CJ, et al. Effects of crude aqueous medicinal plant extracts on growth and invasion of breast cancer cells. Oncol Rep. 2007;17(6):1487-1492. doi:10.3892/or.17.6.1487 36. Vemuri SK, Banala RR, Subbaiah GPV, Srivastava SK, Reddy AVG, Malarvili T. Anti-cancer potential of a mix of natural extracts of turmeric, ginger and garlic: A cell-based study. Egypt J Basic Appl Sci. 2017;4(4):332-344. doi:10.1016/j.ejbas.2017.07.005 37. Hashemi Sheikh Shabani S, Seyed Hasan Tehrani S, Rabiei Z, Tahmasebi Enferadi S, Vannozzi GP. Peganum harmala L.’s anti-growth effect on a breast cancer cell line. Biotechnol Reports. 2015;8:138-143. doi:10.1016/j.btre.2015.08.007 38. Zhang Y, Xie WP, Zhang YK, et al. Experimental study of inhibitory effects of diallyl trisulfide on the growth of human osteosarcoma saos-2 cells by downregulating expression of glucose-regulated protein 78. Onco Targets Ther. 2018;11:271-277. doi:10.2147/OTT.S150933 39. Luo Z, Li D, Luo X, et al. Curcumin may serve an anticancer role in human osteosarcoma cell line U-2 OS by targeting ITPR1. Oncol Lett. 2018;15(4):5593-5601. doi:10.3892/ol.2018.8032 40. Walters DK, Muff R, Langsam B, Born W, Fuchs B. Cytotoxic effects of curcumin on osteosarcoma cell lines. Invest New Drugs. 2008;26(4):289-297. doi:10.1007/s10637-007-9099-7
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Details

Primary Language English
Subjects Traditional, Complementary and Integrative Medicine
Journal Section Research Articles
Authors

Kağan Ağan This is me 0000-0003-1472-570X

Roshanak Zarringhalami This is me 0000-0003-0104-2395

Aydan Fülden Ağan 0000-0002-4319-6264

Mustafa Yaşar This is me 0000-0002-3746-5042

Publication Date April 30, 2020
Submission Date April 15, 2020
Published in Issue Year 2020 Volume: 1 Issue: 1

Cite

APA Ağan, K., Zarringhalami, R., Ağan, A. F., Yaşar, M. (2020). Anticancer Effect of Food Supplements on Saos-2 Osteosarcoma Cell. International Journal of Traditional and Complementary Medicine Research, 1(1), 18-24.

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