Evaluation of LC-MS/MS Analysis and In Vitro Biological Activities of Rosa pimpinellifolia Root, Pseudo-fruit, and Seed extracts

Leyla Güven; Ufuk Özgen; Handan Sevindik; İclal Ağan; Mehmet Koca; İbrahim Turan; Selim Demir; Yüksel Aliyazıcıoğlu

doi:10.38001/ijlsb.1108547

Research Article

Evaluation of LC-MS/MS Analysis and In Vitro Biological Activities of Rosa pimpinellifolia Root, Pseudo-fruit, and Seed extracts

Year 2022, Volume: 5 Issue: 3, 480 - 503, 15.12.2022

Leyla Güven Ufuk Özgen Handan Sevindik İclal Ağan Mehmet Koca İbrahim Turan Selim Demir Yüksel Aliyazıcıoğlu

https://doi.org/10.38001/ijlsb.1108547

Cited By: 2

Abstract

In this study, we investigated antioxidant, anticholinesterase, antityrosinase properties, and polyphenolic and flavonoid content by spectrophotometric; antimicrobial by disc diffusion and microdilution method; antigenotoxic activities by comet assay, and the phytochemical composition of the Rosa pimpinellifolia extracts by LC-MS/MS. The EtOAc extract of the root (RPKE) had higher antioxidant activities at 10 μg/mL with inhibition of 39.7, 91.2, and 39.5% respectively in the DPPH·, ABTS•+, and superoxide anion radical scavenging activity assay than standard antioxidant molecules. The polyphenolic contents of the RPKE and the EtOAc extract of the seed (RPÇE) were found to be 378.2 ± 0.477 and 305.39 ± 0.568 μg GAE/mg respectively. The extracts of RPKE showed butyrylcholinesterase activity with 19±0.12% inhibition at 100 μg/mL concentration. RPKE showed higher activity at 500 μg/mL with inhibition of 50% in the tyrosinase inhibitory assay than the other R. pimpinellifolia extracts. The extracts of R. pimpinellifolia exhibited antimicrobial activity against Staphylococcus aureus and Candida albicans. The extracts of R. pimpinellifolia did not show any antigenotoxic effect up to the concentration of 1000 μg/mL. In LC/MS/MS analysis, Cyanidin-3-O-Glucoside and isoquercetin in the pseudo-fruit; procyanidin B2 and catechin in the root were the major phenolic compounds.

Keywords

Biological Activities, LC/MS/MS, Rosa pimpinellifolia, Rosaceae

References

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Year 2022, Volume: 5 Issue: 3, 480 - 503, 15.12.2022

Leyla Güven Ufuk Özgen Handan Sevindik İclal Ağan Mehmet Koca İbrahim Turan Selim Demir Yüksel Aliyazıcıoğlu

https://doi.org/10.38001/ijlsb.1108547

Cited By: 2

Abstract

References

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6. Gao, X.M., et al., Phenylethanoids from the flowers of Rosa rugosa and their biological activities. Bulletin of the Korean Chemical Society, 2013. 34(1): p. 246-248.
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9. Hashidoko, Y., The phytochemistry of Rosa rugosa. Phytochemistry, 1996. 43(3): p. 535-549.
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17. Nagaki, M., et al., Composition and antimicrobial activity of the essential oil and water extract from Japanese wild Rosa rugosa. Transactions of the Materials Research Society of Japan, 2011. 36(3): p. 517-521.
18. Lee, H.J., et al., Anti-inflammatory effects of hexane fraction from white rose flower extracts via inhibition of inflammatory repertoires. Biomolecules & Therapeutics, 2011. 19(3): p. 331-335.
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26. Seto, T., I. Yasuda, and K. Akiyama, Purgative activity and principals of the fruits of Rosa multiflora and R. wichuraiana. Chemical and Pharmaceutical Bulletin, 1992. 40(8): p. 2080-2082.
27. Tayefi-Nasrabadi, H., S. Sadigh-Eteghad, and Z. Aghdam, The effects of the hydroalcohol extract of Rosa canina L. Fruit on experimentally nephrolithiasic wistar rats. Phytotherapy Research, 2012. 26(1): p. 78-85.
28. Mandade, R.J., et al., Role of the Rosa canina L. leaf extract as an antidiarrheal drug in rodents. Indian Journal of Pharmacology, 2011. 43(3): p. 316-319.
29. Jeon, J.H., et al., Anti-allergic effects of white rose petal extract and anti-atopic properties of its hexane fraction. Archives of Pharmacal Research, 2009. 32(6): p. 823-830.
30. Tumbas, V.T., et al., Effect of rosehip (Rosa canina L.) phytochemicals on stable free radicals and human cancer cells. Journal of the Science of Food and Agriculture, 2012. 92(6): p. 1273-1281.
31. Jung, H.J., et al., 19α-hydroxyursane-type triterpenoids: antinociceptive anti-inflammatory principles of the roots of Rosa rugosa. Biological and Pharmaceutical Bulletin, 2005. 28(1): p. 101-104.
32. Gürbüz, İ., et al., Anti-ulcerogenic activity of some plants used as folk remedy in Turkey. Journal of Ethnopharmacology, 2003. 88(1): p. 93-97.
33. Yoshizawa Y, Kawaii S, Sato T, Murofushi N, Nishimura H. Anticancer and anti-hypertensive effects of small fruit juices, American Chemical Society, 2001,2: 8-23.
34. Trovato, A., et al., In vitro anti-mycotic activity of some medicinal plants containing flavonoids. Bollettino Chimico Farmaceutico, 2000. 139(5): p. 225-227.
35. Boskabady, M.H., et al., Pharmacological effects of Rosa damascena. Iranian Journal of Basic Medical Sciences, 2011. 14(4): p. 295-307.
36. Vlachojannis JE, Duke RK, Tran VH, Duke CC, Chrubasik S. Medicinal properties of Rosa canina, Studium Press, 2010, 27: 441-463.
37. Vertuani, S., et al., Antioxidant herbal supplements for hemorrhoids developing a new formula. Nutrafoods, 2004. 3(3): p. 19-26.
38. Ghasemzadeh, A. and N. Ghasemzadeh, Flavonoids and phenolic acids: Role and biochemical activity in plants and human. Journal of medicinal plants research, 2011. 5(31): p. 6697-6703.
39. Park, H.-R., et al., Antioxidant activity of extracts from Acanthopanax senticosus. African Journal of Biotechnology, 2006. 5(23).
40. Aliyazicioglu, R., et al., Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec. Nat. Prod., 2017. 11(3): p. 270-284.
41. Gyawali, R. and S.A. Ibrahim, Natural products as antimicrobial agents. Food Control, 2014. 46: p. 412-429.
42. Bao, K., et al., Design and synthesis of biphenyl derivatives as mushroom tyrosinase inhibitors. Bioorg. Med. Chem., 2010. 18(18): p. 6708-6714.
43. Masuda, T., et al., Screening for tyrosinase inhibitors among extracts of seashore plants and identification of potent inhibitors from Garcinia subelliptica. Biosci., Biotechnol., Biochem., 2005. 69(1): p. 197-201.
44. Sezer Senol, F., et al., Memory-vitalizing effect of twenty-five medicinal and edible plants and their isolated compounds. S. Afr. J. Bot., 2016. 102: p. 102-109.
45. Tsong-Min, C., Tyrosinase and tyrosinase inhibitors. Journal of Biocatalysis & Biotransformation, 2012.
46. Gholamhoseinian, A. and Z. Razmi, Screening the methanolic extracts of some plants for tyrosinase inhibitory activity. Toxicological & Environmental Chemistry, 2012. 94(2): p. 310-318.
47. Shimizu, K., R. Kondo, and K. Sakai, Inhibition of tyrosinase by flavonoids, stilbenes, and related 4-substituted resorcinols. Structure-activity investigations. Planta Med., 2000. 66(1): p. 11-15.
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Details

Primary Language	English
Subjects	Biochemistry and Cell Biology (Other)
Journal Section	Research Articles
Authors	Leyla Güven 0000-0002-3189-6415 Ufuk Özgen 0000-0001-9839-6717 Handan Sevindik 0000-0003-2738-6874 İclal Ağan Mehmet Koca 0000-0002-1517-5925 İbrahim Turan 0000-0003-3400-5494 Selim Demir 0000-0002-1863-6280 Yüksel Aliyazıcıoğlu 0000-0001-9474-4307
Early Pub Date	May 14, 2022
Publication Date	December 15, 2022
Published in Issue	Year 2022 Volume: 5 Issue: 3

Cite

EndNote	Güven L, Özgen U, Sevindik H, Ağan İ, Koca M, Turan İ, Demir S, Aliyazıcıoğlu Y (December 1, 2022) Evaluation of LC-MS/MS Analysis and In Vitro Biological Activities of Rosa pimpinellifolia Root, Pseudo-fruit, and Seed extracts. International Journal of Life Sciences and Biotechnology 5 3 480–503.

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