Research Article
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The preventive role of different doses Spirulina platensis on lipid peroxidation and antioxidant status in healthy rats

Year 2020, Volume: 4 Issue: 3, 90 - 95, 30.12.2020

Deniz Karakcı Nilay Seyidoğlu Oğuz Merhan Kadir Bozukluhan

https://doi.org/10.30704/http-www-jivs-net.793250

Abstract

There are several antioxidant supplements using for reproductivity and life quality, especially herbal ones. Nowadays, herbal antioxidants especially Spirulina platensis has been still interested due to protective role on oxidant antioxidant balance and health. The present study, we aimed to evaluate the effects of different doses of S.platensis on important oxidant molecule MDA (TBA, oxidant malondialdeyhde), and individual antioxidants as GPx (glutathione peroxidase), CAT (catalase) and SOD (superoxide dismutase) in healthy rats. For this purpose, we used thirty Wistar Albino male rats in three groups: Control, Low Dose Spirulina (500 mg kg-1) and High Dose Spirulina (1000 mg kg-1). S.platensis additives were given by oral gavage daily under a long forty five day of trial. At the end of the study, interestingly, all the antioxidants GPx, CAT, SOD and the oxidant MDA lipid peroxidation values were decreased in group high dose Spirulina compared to Control (p < 0.05). In spite of these decreases, testis weights and indexes were increased in group high dose Spirulina compared to Control significantly. The testis weights and indexes evaluated for normal health of animals. It can be considered that due to the excessive protein and antioxidants features of S.platensis, oxidant and antioxidant mechanisms may be changed. However it can be said that Spirulina can compensate the homeostasis and health of animals. It is also considered that the applications and different doses of S.platensis are needed to be assayed for further studies.

Keywords

Antioxidant Spirulina platensis rat testis index

Supporting Institution

NKUBAP.10.GA.16.074

Project Number

NKUBAP.10.GA.16.074

References

  • Abdel-Daim, M.M., Abuzead, S.M. & Halawa S.M. (2013). Protective role of Spirulina platensis against acute deltamethrin-induced toxicity in rats. Plos One, 8(9), e72991.
  • Afkhami-Arkadani, M., Hasanzadeh, S., Shahrooz, R., Delirezh, N. & Malekinejad, H. (2018). Antioxidant effects of Spirulina platensis (Arthrospira platensis) on cyclophosphamide-induced testicular injury in rats. Veterinary Research Forum, 9, 35-41.
  • Aitken, R.J. & Roman, S.D. (2008). Antioxidant systems and oxidative stress in the testes. Oxidative Medicine and Cellular Longevity, 1, 15-24.
  • Bashandy, S.A.E., El Awdan, S.A., Ebaid, H. & Alhazza, I.M. (2016). Antioxidant Potential of Spirulina platensis Mitigates Oxidative Stress and Reprotoxicity Induced by Sodium Arsenite in Male Rats. Oxidative Medicine and Cellular Longevity, 2016, 7174351.
  • Devasagayam, T., Tilak, J., Boloor, K., Sane, K.S., Ghaskadbi, S.S. & Lele, R. (2004). Free radicals and antioxidants in human health: current status and future prospects. Journal of the Association of Physicians of India, 52, 4-10.
  • Firat, O., Cogun, H., Yuzereroglu, T., Gok, G., Kargin, F. & Kotemen, Y. (2011). A comparative study on the effects of a pesticide (cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus. Fish Physiology and Biochemistry, 37, 657-666.
  • Goksan, T. & Kilic, C. (2009). Growth and biochemical composition of Spirulina platensis Geitler in summer period under the conditions of Çanakkale Turkey. Asian Journal of Chemistry, 21, 4947-4950.
  • Greaves, P. (2007). Male Genital Tract. In: Histopathology of Preclinical Toxicity Studies. 3rd ed. (661-716). USA: Elsevier Science Press.
  • Halliwell, B. & Whiteman, M. (2004). Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?. British Journal of Pharmacology, 142(2), 231–255.
  • Hodges, D.M., DeLong, J.M., Forney, C.F. & Prange, R.K. (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207, 604-611.
  • Ibañez, E., Herrero, M., Mendiola, J.A. & Castro-Puyana, M. (2012). Extraction and characterization of bioactive compounds with health benefits from marine resources: macro and micro algae, cyanobacteria, and invertebrates. In Marine Bioactive Compounds. (pp. 55-98). Springer.
  • Kalafati, M., Jamurtas, A.Z., Nikolaidis, M.G., Paschalis, V., Theodorou, A.A., Sakellariou, G.K., Koutedakis, Y. & Kouretas, D. (2010). Ergogenic and antioxidant effects of spirulina supplementation in humans. Medicine & Sciences in Sports & Exercise, 42, 142-151.
  • Karkos, P.D., Leong, S.C., Karkos, C.D., Sivaji, N. & Assimakopoulos, D.A. (2011). Spirulina in clinical practice: evidence-based human applications. Evidence-Based Complementary and Alternative Medicine, 2011, 1-4.
  • Kitada, K., Macmudah, S., Sasaki, M., Goto, M., Nakashima, Y., Kumamoto, S. & Hasegawa, T. (2009). Antioxidant and antibacterial activity of nutraceutical compounds from Chlorella vulgaris extracted in hydrothermal condition. Separation Science and Technology, 44, 1228-1239.
  • Mahdi, T., Akineh, Y., Ghodrat, R.M., Mojtaba, N. & Soleiman, M. (2019). The effect of Spirulina platensis meal on antioxidant gene expression, total antioxidant capacity, and lipid peroxidation of rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 45, 977-986.
  • Mansour, N., Mcniven, M. & Richardson, G. (2006). The effect of dietary supplementation with blueberry, a-tocopherol or astaxanthin on oxidative stability of Arctic char (Salvelinus alpinus) semen. Theriogenology, 66, 373-382.
  • Martin, D.A., Afonso, L.O., Hosoya, S., Lewis-Mccrea, L.M., Valente, L.M. & Lall, S.P. (2007). Effects of moderately oxidized dietary lipid and the role of vitamin E on the stress response in Atlantic halibut (Hippoglossus hippoglossus L.). Aquaculture, 272, 573-580.
  • Misra, S. & Niyogi, S. (2009). Selenite causes cytotoxicity in rainbow trout (Oncorhynchusmykiss) hepatocytes by inducing oxidative stress. Toxicology In Vitro, 23, 1249-1258.
  • Mohan, I.K., Khan, M., Shobha, J.C., Naidu, M.U., Prayag, A., Kuppusamy, P. & Kutula, V.K. (2006). Protection against cisplatin-induced nephrotoxicity by Spirulina in rats. Cancer Chemotherapy Pharmacology, 58, 802-808.
  • Moreira, L.M., Rocha, A.S.R., Ribeiro, C.L.G., Rodrigues, R.S. & Soares, L.S. (2011). Nutritional evaluation of single-cell protein produced by Spirulina platensis. African Journal of Food Science, 5, 799-805.
  • Nagaoka, S., Shimizu, K., Kaneko, H., Shibayama, F., Morikaw, K., Kanamaru, Y., Otsuka, A., Hirahashi, T. & Toshimitsu, K. (2005). A novel protein C-phycocyanin plays a crucial role in the hypocholesterolemic action of Spirulina platensis concentrate in rats. Journal of Nutrition, 135, 2425-2430.
  • Sarkar, M., Chaudhuri, G.R., Chattopadhyay, A. & Biswas, N.M. (2003). Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian Journal of Andrology, 5, 27-31.
  • Seyidoglu, N., Gurbanli, R., Koseli, E., Cengiz, F. & Aydin, C. (2019). The effects of Spirulina (Arthrospira) platensis on morphological and hematological parameters evoked by social stress in male rats. Journal of Istanbul Veterinary Sciences, 3, 21-27.
  • Urso, M.L. & Clarkson, P.M. (2003). Oxidative stress, exercise and antioxidant supplementation. Toxicology, 189, 41-54.
  • Vernet, P., Aitken, R.J. & Drevet, J.R. (2004). Antioxidant strtategies in the epidydimis. Molecular Cellular Endocrinology, 216, 31-39.
  • Yang, L. & Zhang L.M. (2009). Chemical structural and chain conformational characterization of some bioactive polysaccharides isolated from natural sources. Carbohydrate polymers, 76, 349-361. Yoshoiko, T., Kawada, K. & Shimada, T. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against active-oxygen toxicity in the blood. American Journal of Obstetrics & Gynecology, 135, 372-376.
  • Yu, W., Wen, G., Lin, H., Yang, Y., Huang, X., Zhou, C., Zhang, Z., Duan, Y., Huang, Z. & Li, T. (2018). Effects of dietary Spirulina platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance of Coral trout Plectropomus leopardus (Lacepede, 1802). Fish and Shellfish Immunology, 74, 649-655.
  • Yusuf, M.S., Hassan, M.A., Abdel-Daim, M.M., El Nabtiti, A.S., Ahmed, A.M., Moawed, S.A., El-Sayed, A.K. & Cui, H. (2016). Value added by Spirulina platensis in two different diets on growth performance, gut microbiota, and meat quality of Japanese quails.Veterinary World, 9,1287-1293.
  • Wu, Q., Liu, L., Miron, A., Klimova, B., Wan, D. & Kuca, K. (2016). The antioxidant, immuno-modulatory, and anti-inflammatory activities of Spirulina: An overview. Archive Toxicology, 90, 1-24.

Year 2020, Volume: 4 Issue: 3, 90 - 95, 30.12.2020

Deniz Karakcı Nilay Seyidoğlu Oğuz Merhan Kadir Bozukluhan

https://doi.org/10.30704/http-www-jivs-net.793250

Abstract

Project Number

NKUBAP.10.GA.16.074

References

  • Abdel-Daim, M.M., Abuzead, S.M. & Halawa S.M. (2013). Protective role of Spirulina platensis against acute deltamethrin-induced toxicity in rats. Plos One, 8(9), e72991.
  • Afkhami-Arkadani, M., Hasanzadeh, S., Shahrooz, R., Delirezh, N. & Malekinejad, H. (2018). Antioxidant effects of Spirulina platensis (Arthrospira platensis) on cyclophosphamide-induced testicular injury in rats. Veterinary Research Forum, 9, 35-41.
  • Aitken, R.J. & Roman, S.D. (2008). Antioxidant systems and oxidative stress in the testes. Oxidative Medicine and Cellular Longevity, 1, 15-24.
  • Bashandy, S.A.E., El Awdan, S.A., Ebaid, H. & Alhazza, I.M. (2016). Antioxidant Potential of Spirulina platensis Mitigates Oxidative Stress and Reprotoxicity Induced by Sodium Arsenite in Male Rats. Oxidative Medicine and Cellular Longevity, 2016, 7174351.
  • Devasagayam, T., Tilak, J., Boloor, K., Sane, K.S., Ghaskadbi, S.S. & Lele, R. (2004). Free radicals and antioxidants in human health: current status and future prospects. Journal of the Association of Physicians of India, 52, 4-10.
  • Firat, O., Cogun, H., Yuzereroglu, T., Gok, G., Kargin, F. & Kotemen, Y. (2011). A comparative study on the effects of a pesticide (cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus. Fish Physiology and Biochemistry, 37, 657-666.
  • Goksan, T. & Kilic, C. (2009). Growth and biochemical composition of Spirulina platensis Geitler in summer period under the conditions of Çanakkale Turkey. Asian Journal of Chemistry, 21, 4947-4950.
  • Greaves, P. (2007). Male Genital Tract. In: Histopathology of Preclinical Toxicity Studies. 3rd ed. (661-716). USA: Elsevier Science Press.
  • Halliwell, B. & Whiteman, M. (2004). Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?. British Journal of Pharmacology, 142(2), 231–255.
  • Hodges, D.M., DeLong, J.M., Forney, C.F. & Prange, R.K. (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207, 604-611.
  • Ibañez, E., Herrero, M., Mendiola, J.A. & Castro-Puyana, M. (2012). Extraction and characterization of bioactive compounds with health benefits from marine resources: macro and micro algae, cyanobacteria, and invertebrates. In Marine Bioactive Compounds. (pp. 55-98). Springer.
  • Kalafati, M., Jamurtas, A.Z., Nikolaidis, M.G., Paschalis, V., Theodorou, A.A., Sakellariou, G.K., Koutedakis, Y. & Kouretas, D. (2010). Ergogenic and antioxidant effects of spirulina supplementation in humans. Medicine & Sciences in Sports & Exercise, 42, 142-151.
  • Karkos, P.D., Leong, S.C., Karkos, C.D., Sivaji, N. & Assimakopoulos, D.A. (2011). Spirulina in clinical practice: evidence-based human applications. Evidence-Based Complementary and Alternative Medicine, 2011, 1-4.
  • Kitada, K., Macmudah, S., Sasaki, M., Goto, M., Nakashima, Y., Kumamoto, S. & Hasegawa, T. (2009). Antioxidant and antibacterial activity of nutraceutical compounds from Chlorella vulgaris extracted in hydrothermal condition. Separation Science and Technology, 44, 1228-1239.
  • Mahdi, T., Akineh, Y., Ghodrat, R.M., Mojtaba, N. & Soleiman, M. (2019). The effect of Spirulina platensis meal on antioxidant gene expression, total antioxidant capacity, and lipid peroxidation of rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 45, 977-986.
  • Mansour, N., Mcniven, M. & Richardson, G. (2006). The effect of dietary supplementation with blueberry, a-tocopherol or astaxanthin on oxidative stability of Arctic char (Salvelinus alpinus) semen. Theriogenology, 66, 373-382.
  • Martin, D.A., Afonso, L.O., Hosoya, S., Lewis-Mccrea, L.M., Valente, L.M. & Lall, S.P. (2007). Effects of moderately oxidized dietary lipid and the role of vitamin E on the stress response in Atlantic halibut (Hippoglossus hippoglossus L.). Aquaculture, 272, 573-580.
  • Misra, S. & Niyogi, S. (2009). Selenite causes cytotoxicity in rainbow trout (Oncorhynchusmykiss) hepatocytes by inducing oxidative stress. Toxicology In Vitro, 23, 1249-1258.
  • Mohan, I.K., Khan, M., Shobha, J.C., Naidu, M.U., Prayag, A., Kuppusamy, P. & Kutula, V.K. (2006). Protection against cisplatin-induced nephrotoxicity by Spirulina in rats. Cancer Chemotherapy Pharmacology, 58, 802-808.
  • Moreira, L.M., Rocha, A.S.R., Ribeiro, C.L.G., Rodrigues, R.S. & Soares, L.S. (2011). Nutritional evaluation of single-cell protein produced by Spirulina platensis. African Journal of Food Science, 5, 799-805.
  • Nagaoka, S., Shimizu, K., Kaneko, H., Shibayama, F., Morikaw, K., Kanamaru, Y., Otsuka, A., Hirahashi, T. & Toshimitsu, K. (2005). A novel protein C-phycocyanin plays a crucial role in the hypocholesterolemic action of Spirulina platensis concentrate in rats. Journal of Nutrition, 135, 2425-2430.
  • Sarkar, M., Chaudhuri, G.R., Chattopadhyay, A. & Biswas, N.M. (2003). Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian Journal of Andrology, 5, 27-31.
  • Seyidoglu, N., Gurbanli, R., Koseli, E., Cengiz, F. & Aydin, C. (2019). The effects of Spirulina (Arthrospira) platensis on morphological and hematological parameters evoked by social stress in male rats. Journal of Istanbul Veterinary Sciences, 3, 21-27.
  • Urso, M.L. & Clarkson, P.M. (2003). Oxidative stress, exercise and antioxidant supplementation. Toxicology, 189, 41-54.
  • Vernet, P., Aitken, R.J. & Drevet, J.R. (2004). Antioxidant strtategies in the epidydimis. Molecular Cellular Endocrinology, 216, 31-39.
  • Yang, L. & Zhang L.M. (2009). Chemical structural and chain conformational characterization of some bioactive polysaccharides isolated from natural sources. Carbohydrate polymers, 76, 349-361. Yoshoiko, T., Kawada, K. & Shimada, T. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against active-oxygen toxicity in the blood. American Journal of Obstetrics & Gynecology, 135, 372-376.
  • Yu, W., Wen, G., Lin, H., Yang, Y., Huang, X., Zhou, C., Zhang, Z., Duan, Y., Huang, Z. & Li, T. (2018). Effects of dietary Spirulina platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance of Coral trout Plectropomus leopardus (Lacepede, 1802). Fish and Shellfish Immunology, 74, 649-655.
  • Yusuf, M.S., Hassan, M.A., Abdel-Daim, M.M., El Nabtiti, A.S., Ahmed, A.M., Moawed, S.A., El-Sayed, A.K. & Cui, H. (2016). Value added by Spirulina platensis in two different diets on growth performance, gut microbiota, and meat quality of Japanese quails.Veterinary World, 9,1287-1293.
  • Wu, Q., Liu, L., Miron, A., Klimova, B., Wan, D. & Kuca, K. (2016). The antioxidant, immuno-modulatory, and anti-inflammatory activities of Spirulina: An overview. Archive Toxicology, 90, 1-24.
There are 29 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Articles
Authors

Deniz Karakcı 0000-0002-1884-1874

Nilay Seyidoğlu 0000-0002-2817-5131

Oğuz Merhan 0000-0002-3399-0667

Kadir Bozukluhan 0000-0003-4929-5156

Project Number NKUBAP.10.GA.16.074
Publication Date December 30, 2020
Published in Issue Year 2020 Volume: 4 Issue: 3

Cite

APA Karakcı, D., Seyidoğlu, N., Merhan, O., Bozukluhan, K. (2020). The preventive role of different doses Spirulina platensis on lipid peroxidation and antioxidant status in healthy rats. Journal of Istanbul Veterinary Sciences, 4(3), 90-95. https://doi.org/10.30704/http-www-jivs-net.793250

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