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Dianthus carmelitarum Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin İncelenmesi

Year 2017, Volume: 7 Issue: 1, 41 - 50, 31.01.2017

Selim Demir İbrahim Turan Rezzan Aliyazıcıoğlu Sema Mısır Yüksel Aliyazicioglu

Abstract

Dianthus carmelitarum Reut. Ex Boiss, Dianthus cinsi ve Caryophyllaceae familyasına mensup bir bitki olup, bu cins bitkiler sıklıkla geleneksel tedavide kullanılmaktadırlar. Farklı Dianthus türlerinin farklı ekstraktlarının antioksidan ve sitotoksik etkilerini konu alan çok sayıda çalışma olmasına rağmen, D. carmelitarum ekstraktlarının bu özellikleri ile ilgili herhangi bir çalışma bulunmamaktadır. Bu çalışmanın amacı D. carmelitarum’un dimetil sülfoksitli ekstraktının antioksidan özelliklerini ve sitotoksik etkilerini belirlemektir. Ekstraktın toplam fenolik madde miktarı, toplam flavonoid madde miktarı ve indirgeyici antioksidan güç tayini spektrofotometrik yöntemler kullanılarak belirlendi. Ekstraktın sitotoksik etkisi ise insan serviks (HeLa), akciğer (A549), meme (MCF-7) ve prostat (PC-3) kanser hücre serilerinde MTT yöntemi kullanılarak değerlendirildi. Ekstraktın toplam fenolik madde içeriği ve indirgeyici antioksidan güç değerleri g örnek başına sırasıyla 853.9±19.4 mg gallik asit eşdeğeri ve 1378.7±87.4 mg troloks eşdeğeri olarak bulundu. Ekstrakt çalışılan tüm kanser hücre serileri üzerinde sitotoksik etki gösterdi ve IC50 değerleri 117.4-142.1 µg/mL arasında hesaplandı. Bu çalışma D. carmelitarum ekstraktının in vitro koşullarda kanser hücre serileri üzerindeki sitotoksik etkisini ortaya koyan ilk çalışmadır. Ekstrakttaki etken molekülleri ve bunların etki mekanizmalarını belirleyebilmek için daha ileri çalışmalara ihtiyaç vardır.

Keywords

Antioksidan aktivite Dianthus carmelitarum; Kanser; Sitotoksisite

References

  • Aliyazicioglu, Y., Demir, S., Turan, I., Cakiroglu, T.N., Akalin, I., Deger, O. ve Bedir, A., 2011. Preventive and protective effects of Turkish propolis on H2O2 induced DNA damage in foreskin fibroblast cell lines, Acta Biologica Hungarica, 62, 4, 388-396.
  • Cai, Y., Luo, Q., Sun, M. ve Corke, H., 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer, Life Sciences, 74, 2157-2184.
  • Cenic-Milosevic, D., Tambur, Z., Bokonjic, D., Ivancajic, S., Stanojkovic, T., Grozdanic, N. ve Juranic, Z., 2013. Antiproliferative effects of some medicinal plants on HeLa cells, Archives of Biological Sciences, 65, 1, 65-70.
  • Demir, S,, Aliyazicioglu, Y., Turan, I., Misir, S., Mentese, A., Ozer Yaman, S., Akbulut, K., Kilinc, K. ve Deger, O., 2016a. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line, Nutrition and Cancer, 68, 1, 165-172.
  • Demir, S., Turan, I. ve Aliyazicioglu, Y., 2016b. Selective cytotoxic effect of Rhododendron luteum extract on human colon and liver cancer cells, Journal of Balkan Union of Oncology, 21, 4, 883-888.
  • Ding, C., Zhang, W., Li, J., Lei, J. ve Yu, J., 2013. Cytotoxic constituents of ethyl acetate fraction from Dianthus superbus, Natural Product Research, 27, 18, 1691-1694.
  • Gou, J., Zou, Y. ve Ahn, J., 2011. Enhancement of antioxidant and antimicrobial activities of Dianthus superbus, Polygonum aviculare, Sophora flavescens, and Lygodium japonicum by pressure-assisted water extraction, Food Science and Biotechnology, 20, 1, 283-287.
  • Hamzaoglu, E., Koc, M., Buyuk, I., Aksoy, A. ve Soydam Aydin, S., 2015. Presence of Dianthus roseoluteus Velen. (Caryophyllaceae) in Turkey and a new species: Dianthus macroflorus Hamzaoglu, Systematic Botany, 40, 208-213.
  • Hsieh, P.W., Chang, F.R., Wu, C.C., Wu, K.Y., Li, C.M., Chen, S.L. ve Wu, Y.C., 2004. New cytotoxic cyclic peptides and dianthramide from Dianthus superbus, Journal of Natural Products, 67, 1522-1527.
  • Hsieh, P.W., Chang, F.R., Wu, C.C., Li, C.M., Wu, K.Y., Chen, S.L., Yen, H.F. ve Wu, Y.C., 2005. Longicalycinin A, a new cytotoxic cyclic peptide from Dianthus superbus var. longicalycinus (MAXIM.) WILL, Chemical and Pharmaceutical Bulletin, 53, 3, 336-338.
  • Huang, W.Y. ve Cai, Y.Z., 2010. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention, Nutrition and Cancer, 62, 1, 1-20.
  • Kathiresan, K., Boopathy, N.S. ve Kavitha, S., 2006. Coastal vegetation-an underexplored source of anticancer drugs, Natural Product Radiance, 5, 115-119.
  • Kaur, C. ve Kapoor, H.C., 2001. Antioxidants in fruits and vegetables-the millennium's health, International Journal of Food Science & Technology, 36, 703-725.
  • Kazeem, M.I. ve Ashafa, A.O.T., 2015. In-vitro antioxidant and antidiabetic potentials of Dianthus basuticus Burtt Davy whole plant extracts, Journal of Herbal Medicine, 5, 158-164.
  • Lamula, S.Q.N. ve Ashafa, A.O.T., 2014. Antimicrobial and cytotoxic potential of Dianthus basuticus used in Basotho traditional practice, Bangladesh Journal of Pharmacology, 9, 105-111.
  • Lee, J., Seo, Y., Lee, J. ve Ju, J., 2016. Antioxidant activities of Dianthus chinensis L. extract and its inhibitory activities against nitric oxide production and cancer cell growth and adhesion, Journal of the Korean Society of Food Science and Nutrition, 45, 1, 44-51.
  • Li, A.N., Li, S., Zhang, Y.J., Xu, X.R., Chen, Y.M. ve Li, H.B., 2014. Resources and biological activities of natural polyphenols, Nutrients, 6, 12, 6020-6047.
  • Liang, C.H., Wang, G.H., Chou, T.H., Wang, S.H., Lin, R.J., Chan, L.P., So, E.C. ve Sheu, J.H., 2012. 5-epi-Sinuleptolide induces cell cycle arrest and apoptosis through tumor necrosis factor/mitochondria-mediated caspase signaling pathway in human skin cancer cells, Biochimica et Biophysica Acta, 1820, 1149-1157.
  • Martineti, V., Tognarini, I., Azzari, C., Sala, S.C., Clematis, F., Dolci, M., Lanzotti, V., Tonelli, F., Brandi, M.L. ve Curir, P., 2010. Inhibition of in vitro growth and arrest in the G0/G1 phase of HCT8 line human colon cancer cells by kaempferide triglycoside from Dianthus caryophyllus, Phytotherapy Research, 24, 1302-1308.
  • Moreno, M.I., Isla, M.I., Sampietro, A.R. ve Vattuone, M.A., 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina, Journal of Ethnopharmacology, 71, 1-2, 109-114.
  • Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, Journal of Immunological Methods, 65, 55-63.
  • Naghibi, F., Irani, M., Hassanpour, A., Pirani, A. ve Hamzeloo-Moghadam, M., 2014. Cytotoxic effects of selective species of Caryophyllaceae in Iran, Research Journal of Pharmacognosy, 1, 2, 29-32.
  • Nho, K.J., Chun, J.M. ve Kim, H.K., 2012. Ethanol extract of Dianthus chinensis L. induces apoptosis in human hepatocellular carcinoma HepG2 cells in vitro. Evidence-Based Complementary and Alternative Medicine, Article ID: 573527, doi: 10.1155/2012/573527.
  • Nuutila, A.M., Puupponen-Pimia, R., Aarni, M. ve Oksman-Caldentey, K.M., 2003. Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity, Food Chemistry, 81, 485-493.
  • Obmann, A., Zehl, M., Purevsuren, S., Narantuya, S., Reznicek, G., Kletter, C. ve Glasl, S., 2011a. Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolor Fisch, Journal of Seperation Science, 34, 292-298.
  • Obmann, A., Werner, I., Presser, A., Zehl, M., Swoboda, Z., Purevsuren, S., Narantuya, S., Kletter, C. ve Glasl, S., 2011. Flavonoid C- and O-glycosides from the Mongolian medicinal plant Dianthus versicolor Fisch, Carbohydrate Research, 346, 1868-1875.
  • Oyaizu, M., 1986. Studies on product of browning reaction prepared from glucose amine, Japanese Journal of Nutrition, 44, 307-315.
  • Ravishankar, D., Rajora, A.K., Greco, F. ve Osborn, H.M., 2013. Flavonoids as prospective compounds for anti-cancer therapy, International Journal of Biochemistry & Cell Biology, 45, 12, 2821-2831.
  • Reeve, H. 1967, Dianthus L. Flora of Turkey and the East Aegean Islands. Davis, P.H. (ed), Edinburgh University Press, Edinburgh. pp. 99-131.
  • Rosangkima, G. ve Prasad, S.B., 2004. Antitumour activity of some plants from Meghalaya and Mizoram against murine ascites Dolton`s lymphoma, Indian Journal of Experimental Biology, 42, 981-988.
  • Shin, J.A., Kim, J.J., Choi, E.S., Shim, J.H., Ryu, M.H., Kwon, K.H., Park, H.M., Seo, J.Y., Lee, S.Y., Lim, D.W., Cho, N.P. ve Cho, S.D., 2013. In vitro apoptotic effects of methanol extracts of Dianthus chinensis and Acalypha australis L. targeting specificity protein 1 in human oral cancer cells, Head & Neck, 35, 7, 992-998.
  • Slinkard, K. ve Singleton, V.L., 1977. Total phenol analysis: automation and comparison with manual methods, American Journal of Enology and Viticulture, 28, 49-55.
  • Surh, Y.J., 2003. Cancer chemoprevention with dietary phytochemicals. Nature Reviews Cancer, 3, 10, 768-780.
  • Turan, I., Demir, S., Misir, S., Kilinc, K., Mentese, A., Aliyazicioglu, Y. ve Deger, O., 2015a. Cytotoxic effect of Turkish propolis on liver, colon, breast, cervix and prostate cancer cell lines, Tropical Journal of Pharmaceutical Research, 14, 5, 777-782.
  • Turan, I., Deger, O., Aliyazicioglu, Y., Demir, S., Kilinc, K. ve Sumer, A., 2015b. Effects of Turkish propolis on expression of hOGG-1 and NEIL-1, Turkish Journal of Medical Sciences, 45, 804-811.
  • Yu, J.Q., Liao, Z.X., Lei, J.C. ve Hu, X.M., 2007. Antioxidant and cytotoxic activities of various fractions of ethanol extract of Dianthus superbus, Food Chemistry, 104, 1215-1219.
  • Yu, J.Q., Yin, Y., Lei, J.C., Zhang, X.Q., Chen, W., Ding, C.L., Wu, S., He, X.Y., Liu, Y.W. ve Zou, G.L., 2012. Activation of apoptosis by ethyl acetate fraction of ethanol extract of Dianthus superbus in HepG2 cell line, Cancer Epidemiology, 36, e40-e45.

Year 2017, Volume: 7 Issue: 1, 41 - 50, 31.01.2017

Selim Demir İbrahim Turan Rezzan Aliyazıcıoğlu Sema Mısır Yüksel Aliyazicioglu

Abstract

References

  • Aliyazicioglu, Y., Demir, S., Turan, I., Cakiroglu, T.N., Akalin, I., Deger, O. ve Bedir, A., 2011. Preventive and protective effects of Turkish propolis on H2O2 induced DNA damage in foreskin fibroblast cell lines, Acta Biologica Hungarica, 62, 4, 388-396.
  • Cai, Y., Luo, Q., Sun, M. ve Corke, H., 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer, Life Sciences, 74, 2157-2184.
  • Cenic-Milosevic, D., Tambur, Z., Bokonjic, D., Ivancajic, S., Stanojkovic, T., Grozdanic, N. ve Juranic, Z., 2013. Antiproliferative effects of some medicinal plants on HeLa cells, Archives of Biological Sciences, 65, 1, 65-70.
  • Demir, S,, Aliyazicioglu, Y., Turan, I., Misir, S., Mentese, A., Ozer Yaman, S., Akbulut, K., Kilinc, K. ve Deger, O., 2016a. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line, Nutrition and Cancer, 68, 1, 165-172.
  • Demir, S., Turan, I. ve Aliyazicioglu, Y., 2016b. Selective cytotoxic effect of Rhododendron luteum extract on human colon and liver cancer cells, Journal of Balkan Union of Oncology, 21, 4, 883-888.
  • Ding, C., Zhang, W., Li, J., Lei, J. ve Yu, J., 2013. Cytotoxic constituents of ethyl acetate fraction from Dianthus superbus, Natural Product Research, 27, 18, 1691-1694.
  • Gou, J., Zou, Y. ve Ahn, J., 2011. Enhancement of antioxidant and antimicrobial activities of Dianthus superbus, Polygonum aviculare, Sophora flavescens, and Lygodium japonicum by pressure-assisted water extraction, Food Science and Biotechnology, 20, 1, 283-287.
  • Hamzaoglu, E., Koc, M., Buyuk, I., Aksoy, A. ve Soydam Aydin, S., 2015. Presence of Dianthus roseoluteus Velen. (Caryophyllaceae) in Turkey and a new species: Dianthus macroflorus Hamzaoglu, Systematic Botany, 40, 208-213.
  • Hsieh, P.W., Chang, F.R., Wu, C.C., Wu, K.Y., Li, C.M., Chen, S.L. ve Wu, Y.C., 2004. New cytotoxic cyclic peptides and dianthramide from Dianthus superbus, Journal of Natural Products, 67, 1522-1527.
  • Hsieh, P.W., Chang, F.R., Wu, C.C., Li, C.M., Wu, K.Y., Chen, S.L., Yen, H.F. ve Wu, Y.C., 2005. Longicalycinin A, a new cytotoxic cyclic peptide from Dianthus superbus var. longicalycinus (MAXIM.) WILL, Chemical and Pharmaceutical Bulletin, 53, 3, 336-338.
  • Huang, W.Y. ve Cai, Y.Z., 2010. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention, Nutrition and Cancer, 62, 1, 1-20.
  • Kathiresan, K., Boopathy, N.S. ve Kavitha, S., 2006. Coastal vegetation-an underexplored source of anticancer drugs, Natural Product Radiance, 5, 115-119.
  • Kaur, C. ve Kapoor, H.C., 2001. Antioxidants in fruits and vegetables-the millennium's health, International Journal of Food Science & Technology, 36, 703-725.
  • Kazeem, M.I. ve Ashafa, A.O.T., 2015. In-vitro antioxidant and antidiabetic potentials of Dianthus basuticus Burtt Davy whole plant extracts, Journal of Herbal Medicine, 5, 158-164.
  • Lamula, S.Q.N. ve Ashafa, A.O.T., 2014. Antimicrobial and cytotoxic potential of Dianthus basuticus used in Basotho traditional practice, Bangladesh Journal of Pharmacology, 9, 105-111.
  • Lee, J., Seo, Y., Lee, J. ve Ju, J., 2016. Antioxidant activities of Dianthus chinensis L. extract and its inhibitory activities against nitric oxide production and cancer cell growth and adhesion, Journal of the Korean Society of Food Science and Nutrition, 45, 1, 44-51.
  • Li, A.N., Li, S., Zhang, Y.J., Xu, X.R., Chen, Y.M. ve Li, H.B., 2014. Resources and biological activities of natural polyphenols, Nutrients, 6, 12, 6020-6047.
  • Liang, C.H., Wang, G.H., Chou, T.H., Wang, S.H., Lin, R.J., Chan, L.P., So, E.C. ve Sheu, J.H., 2012. 5-epi-Sinuleptolide induces cell cycle arrest and apoptosis through tumor necrosis factor/mitochondria-mediated caspase signaling pathway in human skin cancer cells, Biochimica et Biophysica Acta, 1820, 1149-1157.
  • Martineti, V., Tognarini, I., Azzari, C., Sala, S.C., Clematis, F., Dolci, M., Lanzotti, V., Tonelli, F., Brandi, M.L. ve Curir, P., 2010. Inhibition of in vitro growth and arrest in the G0/G1 phase of HCT8 line human colon cancer cells by kaempferide triglycoside from Dianthus caryophyllus, Phytotherapy Research, 24, 1302-1308.
  • Moreno, M.I., Isla, M.I., Sampietro, A.R. ve Vattuone, M.A., 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina, Journal of Ethnopharmacology, 71, 1-2, 109-114.
  • Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, Journal of Immunological Methods, 65, 55-63.
  • Naghibi, F., Irani, M., Hassanpour, A., Pirani, A. ve Hamzeloo-Moghadam, M., 2014. Cytotoxic effects of selective species of Caryophyllaceae in Iran, Research Journal of Pharmacognosy, 1, 2, 29-32.
  • Nho, K.J., Chun, J.M. ve Kim, H.K., 2012. Ethanol extract of Dianthus chinensis L. induces apoptosis in human hepatocellular carcinoma HepG2 cells in vitro. Evidence-Based Complementary and Alternative Medicine, Article ID: 573527, doi: 10.1155/2012/573527.
  • Nuutila, A.M., Puupponen-Pimia, R., Aarni, M. ve Oksman-Caldentey, K.M., 2003. Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity, Food Chemistry, 81, 485-493.
  • Obmann, A., Zehl, M., Purevsuren, S., Narantuya, S., Reznicek, G., Kletter, C. ve Glasl, S., 2011a. Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolor Fisch, Journal of Seperation Science, 34, 292-298.
  • Obmann, A., Werner, I., Presser, A., Zehl, M., Swoboda, Z., Purevsuren, S., Narantuya, S., Kletter, C. ve Glasl, S., 2011. Flavonoid C- and O-glycosides from the Mongolian medicinal plant Dianthus versicolor Fisch, Carbohydrate Research, 346, 1868-1875.
  • Oyaizu, M., 1986. Studies on product of browning reaction prepared from glucose amine, Japanese Journal of Nutrition, 44, 307-315.
  • Ravishankar, D., Rajora, A.K., Greco, F. ve Osborn, H.M., 2013. Flavonoids as prospective compounds for anti-cancer therapy, International Journal of Biochemistry & Cell Biology, 45, 12, 2821-2831.
  • Reeve, H. 1967, Dianthus L. Flora of Turkey and the East Aegean Islands. Davis, P.H. (ed), Edinburgh University Press, Edinburgh. pp. 99-131.
  • Rosangkima, G. ve Prasad, S.B., 2004. Antitumour activity of some plants from Meghalaya and Mizoram against murine ascites Dolton`s lymphoma, Indian Journal of Experimental Biology, 42, 981-988.
  • Shin, J.A., Kim, J.J., Choi, E.S., Shim, J.H., Ryu, M.H., Kwon, K.H., Park, H.M., Seo, J.Y., Lee, S.Y., Lim, D.W., Cho, N.P. ve Cho, S.D., 2013. In vitro apoptotic effects of methanol extracts of Dianthus chinensis and Acalypha australis L. targeting specificity protein 1 in human oral cancer cells, Head & Neck, 35, 7, 992-998.
  • Slinkard, K. ve Singleton, V.L., 1977. Total phenol analysis: automation and comparison with manual methods, American Journal of Enology and Viticulture, 28, 49-55.
  • Surh, Y.J., 2003. Cancer chemoprevention with dietary phytochemicals. Nature Reviews Cancer, 3, 10, 768-780.
  • Turan, I., Demir, S., Misir, S., Kilinc, K., Mentese, A., Aliyazicioglu, Y. ve Deger, O., 2015a. Cytotoxic effect of Turkish propolis on liver, colon, breast, cervix and prostate cancer cell lines, Tropical Journal of Pharmaceutical Research, 14, 5, 777-782.
  • Turan, I., Deger, O., Aliyazicioglu, Y., Demir, S., Kilinc, K. ve Sumer, A., 2015b. Effects of Turkish propolis on expression of hOGG-1 and NEIL-1, Turkish Journal of Medical Sciences, 45, 804-811.
  • Yu, J.Q., Liao, Z.X., Lei, J.C. ve Hu, X.M., 2007. Antioxidant and cytotoxic activities of various fractions of ethanol extract of Dianthus superbus, Food Chemistry, 104, 1215-1219.
  • Yu, J.Q., Yin, Y., Lei, J.C., Zhang, X.Q., Chen, W., Ding, C.L., Wu, S., He, X.Y., Liu, Y.W. ve Zou, G.L., 2012. Activation of apoptosis by ethyl acetate fraction of ethanol extract of Dianthus superbus in HepG2 cell line, Cancer Epidemiology, 36, e40-e45.
There are 37 citations in total.

Details

Journal Section Articles
Authors

Selim Demir

İbrahim Turan

Rezzan Aliyazıcıoğlu

Sema Mısır

Yüksel Aliyazicioglu

Publication Date January 31, 2017
Submission Date October 10, 2016
Published in Issue Year 2017 Volume: 7 Issue: 1

Cite

APA Demir, S., Turan, İ., Aliyazıcıoğlu, R., Mısır, S., et al. (2017). Dianthus carmelitarum Ekstraktının Antioksidan ve Sitotoksik Özelliklerinin İncelenmesi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 7(1), 41-50.
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