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Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi

Year 2020, Volume: 1 Issue: 1-2, 1 - 12, 30.12.2020

Fatma Ünal Nesrin Durdu Helvacı Tülek Deniz Yüzbaşıoğlu Mustafa Çelik

https://doi.org/10.5281/zenodo.4317924

Abstract

Organik fosforlu pestisitlerden olan Methidathion (Supracide 40 EC, MET), meyve ağaçlarında, sebzelerde,
tütünde, yoncada, ayçiçeğinde, seralarda ve gül bahçelerinde çeşitli böcek ve akarlara karşı
kullanılmaktadır. Bu araştırmada, Allium testi kullanılarak, methidathionun Allium cepa kök ucu
hücrelerindeki sitotoksik ve genotoksik etkileri incelenmiştir. Kök büyüme inhibisyon testi ile, etkili
konsantrasyon (EC50) değeri 30 mg/L olarak tespit edildikten sonra, Allium cepa kök uçları, MET’un dört
farklı konsantrasyonu (7,5, 15, 30 ve 45 mg/L) ile 12, 24 ve 48 saat muamele edilmiştir. MET, mitotik indeksi
(MI), tüm konsantrasyonlarda ve uygulama sürelerinde, kontrole kıyasla anlamlı şekilde düşürmüştür. Diğer
yandan MET, kromozomal anormallikleri, kontrole kıyasla anlamlı düzeyde artırmıştır. En yaygın görülen
anormallikler kroımozom yapışıklığı (%47,50) ve C-mitoz (%44,24)’dur. Bunları sırasıyla fragment (%2,75),
köprü (%2,55), geri kalma (%1,63) ve çok kutupluluk (%1,33) takip etmiştir. Çalışmada ayrıca, ön işleme
tabi tutulmuş kök uçlarında kromozom ve kromatid kırıkları, fragmentler ve poliploidi tespit edilmiştir. Bu
çalışmadan elde edilen sonuçlar, MET’un Allium cepa'da belirgin şekilde sitotoksik ve genotoksik olduğunu
göstermiştir. Ayrıca, Allium testinin, MET’un toksik etkilerini belirlemede çok hassas ve etkili bir test
olduğunu doğrulamıştır.

Keywords

Pestisit Methidathion Sitotoksik etki Genotoksik etki Allium cepa

References

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Year 2020, Volume: 1 Issue: 1-2, 1 - 12, 30.12.2020

Fatma Ünal Nesrin Durdu Helvacı Tülek Deniz Yüzbaşıoğlu Mustafa Çelik

https://doi.org/10.5281/zenodo.4317924

Abstract

References

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  • [23] Ünal, F., Demir, H., and Yüzbaşioğlu, D. (2017). Genotoxic effects of environmental contaminant methidathion and triadimenol pesticides. The 3rd International Symposium on EuroAsian Biodiversity 05-08 July 2017, MinskBelarus.
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  • [26] Ma, T. H., Cabrera, G. L., and Owens E. (2005). Genotoxic agents detected by plant bioassays. Reviews on Environmental Health, 20(1), 1-14.
  • [27] Palmieri, M. J., Andrade-Vieira, L. F., Trento, M. V. C., Eleutério, M. W. F., Luber, J., Davide, L. C., et al. (2016). Cytogenotoxic effects of spent pot liner (SPL) and its main components on human leukocytes and meristematic cells of Allium cepa. Water Air and Soil Pollution, 227,156.
  • [28] Vicentini, V. E. P., Camparoto, M. L., Teixeira, R. O., and Mantovani, M. S. (2001). Averrhoa carambola L., Syzygium cumini (L.) Skeels and Cissus sicyoides L.: medicinal herbal tea effects on vegetal and test systems. Acta Scientiarum, 23(2), 593-598.
  • [29] Teixeira, R. O., Camparoto, M. L., Mantovani, M. S., and Vicentini, V. E. P. (2003). Assesment of two medicinal plants Psidium guajava L. and Achillea millefolium L., in vitro and in vivo assays. Genetics and Molecular Biology, 26(4), 551-555.
  • [30] Tedesco, S. B. and Laughinghouse, I. V. H. D. (2012). Bioindicator of genotoxicity: The Allium cepa test. In: Srivastava J, editor. Environmental Contamination. Croatia: InTech, 137-156.
  • [31] Özkul M., Özel Ç.A., Yüzbaşıoğlu D., and Ünal F. (2016). Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots? Cytotechnology, 68, 2395-2405.
  • [32] Doroftei, E., Antofie, M. M., Sava, D., and Arcus, M. (2010). Cytogenetic effects induced by Manganese and Lead micro-elements on germination at Allium cepa. Botanica Serbica, 34(2), 115-121.
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There are 71 citations in total.

Details

Primary Language Turkish
Journal Section Araştırma Makaleleri
Authors

Fatma Ünal This is me

Nesrin Durdu Helvacı Tülek This is me

Deniz Yüzbaşıoğlu

Mustafa Çelik

Publication Date December 30, 2020
Published in Issue Year 2020 Volume: 1 Issue: 1-2

Cite

APA Ünal, F., Helvacı Tülek, N. D., Yüzbaşıoğlu, D., Çelik, M. (2020). Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. Gazi Üniversitesi Fen Fakültesi Dergisi, 1(1-2), 1-12. https://doi.org/10.5281/zenodo.4317924
AMA Ünal F, Helvacı Tülek ND, Yüzbaşıoğlu D, Çelik M. Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. GÜFFD. December 2020;1(1-2):1-12. doi:10.5281/zenodo.4317924
Chicago Ünal, Fatma, Nesrin Durdu Helvacı Tülek, Deniz Yüzbaşıoğlu, and Mustafa Çelik. “Methidathion İnsektisit/Akarisitinin Sitotoksik Ve Genotoksik Potansiyelinin Allium Testi Ile İncelenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi 1, no. 1-2 (December 2020): 1-12. https://doi.org/10.5281/zenodo.4317924.
EndNote Ünal F, Helvacı Tülek ND, Yüzbaşıoğlu D, Çelik M (December 1, 2020) Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. Gazi Üniversitesi Fen Fakültesi Dergisi 1 1-2 1–12.
IEEE F. Ünal, N. D. Helvacı Tülek, D. Yüzbaşıoğlu, and M. Çelik, “Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi”, GÜFFD, vol. 1, no. 1-2, pp. 1–12, 2020, doi: 10.5281/zenodo.4317924.
ISNAD Ünal, Fatma et al. “Methidathion İnsektisit/Akarisitinin Sitotoksik Ve Genotoksik Potansiyelinin Allium Testi Ile İncelenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi 1/1-2 (December 2020), 1-12. https://doi.org/10.5281/zenodo.4317924.
JAMA Ünal F, Helvacı Tülek ND, Yüzbaşıoğlu D, Çelik M. Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. GÜFFD. 2020;1:1–12.
MLA Ünal, Fatma et al. “Methidathion İnsektisit/Akarisitinin Sitotoksik Ve Genotoksik Potansiyelinin Allium Testi Ile İncelenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi, vol. 1, no. 1-2, 2020, pp. 1-12, doi:10.5281/zenodo.4317924.
Vancouver Ünal F, Helvacı Tülek ND, Yüzbaşıoğlu D, Çelik M. Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi. GÜFFD. 2020;1(1-2):1-12.
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