BibTex RIS Cite

ÇİMLENEN FASULYE TOHUMLARINDA α–AMİLAZ ENZİM AKTİVİTESİ VE ERKEN BÜYÜME PARAMETRELERİ ÜZERİNE MEMELİ CİNSİYET HORMONLARININ ETKİLERİ

Year 2010, Volume: 3 Issue: 1, 61 - 71, 11.03.2014

Abstract

Bu çalışmada, memeli cinsiyet hormonları olan progesteron, β-östradiol ve androsteronun çimlenen fasulye tohumlarında erken büyüme parametreleri (çimlenme yüzdesi, kök ve gövde uzunluğu) ve çimlenmede önemli rol oynayan α-amilaz enzim aktivitesi üzerine etkileri araştırılmıştır. Farklı konsantrasyonlarda (10-4, 10-6, 10-9, 10-12, 10-15 M) hazırlanan progesteron, β-östradiol ve androsteron çözeltilerinde yaklaşık 6 saat şişirilen fasulye tohumları 5 gün süreyle çimlendirilmiştir. 1., 3. ve 5. günlerin sonunda yapılan ölçümlerde, bu hormonların çalışılan bütün konsantrasyonlarda çimlenme oranını, kök ve gövde uzamasını ve α-amilaz aktivitesini önemli oranda artırdıkları gözlenmiştir. Tüm parametrelerde maksimum artışlar 10-9 M progesteron, 10-6- 10-9 M β –östradiol ve 10-9 M androsteronda kaydedilmiştir.

References

  • Aisien, A.O. and Stark, J.R. (1983). The development of enzymes during germination and seedling growth in Nigerian sorghum. Staerke. 35:316
  • Barrington, E.J.W. (1979). in: Hormones and Evolution (E.J.W. Barrington, ed.)
  • (pp. VII-XXI). London, Academic Press. Bewley, J.D. and Black M. (1994). Seeds: Physiology of Development and Germination. 2nd ed. (pp 1-31).New York and London, Plenum Press. deMorais, G.A. and Takaki, M. (1998). Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca. Braz. Arch. Biol. Techn., :17-25.
  • Dogra, R. and Kaur, A. (1994). Effect of steroids on some growth and biochemical parameters of Triticum aestivum L. during germination. Crop Res., 8: 611-620.
  • Dogra, R. and Thukral, A. (1991). Effect of steroid and plant hormones on some germination aspects of Triticum aestivum L. In: New trends in plant physiology Physiology (Eds. K. K. Dhir, I. S. Dua and K. S. Chark),
  • (pp. 65-70). New Delhi, Today and Tomorrow's Printers and Publishers. Dohrn, M., Faure, W., Poll, H. and Blotevogel, W. (1926). Tokokinine, Stoff mit sexualhormonartiger Wirkung aus Pflanzenzellen. Med Klin., :1417-1419.
  • Dow, M.A. and Schwintzer, C.R. (1999). Seed germination, seedling emergence, and seed bank ecology of sweet fern (comptonia peregrina (l.) Coult.). Can. J. Bot., 77: 1378-1386.
  • Geuns, J. M. C. (1978) Steroid hormones and plant growth and development. Phytochem. 17, 1–14.
  • Guan, M. and Roddick, J.G. (1988). Comparison of the effects of epibrassinolide and steroidal estrogens on adventitious root-growth and early shoot development in mung bean cuttings. Physiol. Plantarum, 73: 426-431.
  • Guglielminetti, L., Busilacchi, H.A. and Alpi A. (2000). Effect of anoxia on a- amylase induction in maize caryopsis. J. Plant Res., 113:185-92.
  • Heftmann, E. (1975). Steroid hormones in plants. Lloydia, 38:195-209.
  • Helmkamp, G. and Bonner, J. (1952). Some relationships of sterols to plant growth. Plant Physiol., 28:428-436.
  • Janeczko, A. and Skoczowski, A. (2005). Mammalian sex hormones in plants.
  • Folia Histochem. Cyto., 43:71-79. Juliano, B. and Varner, J.E. (1969). Enzymic Degradation of Starch Granules in the Cotyledons of Germinating Peas. Plant Physiol., 44:886-892.
  • Kato-Noguchi, H. and Macias, F.M. (2005). Effects of 6-methoxy-2- benzoxazolinone on the germination and α-amylase activity in lettuce seeds. J. Plant Physiol., 162:1304-1307.
  • Kopcewicz, J. (1969). Influence of estrone on growth and endogenous gibberellin content in dwarf pea. Bull. Acad. Pol. Sci. Cl. II. Ser. Sci. Biol., 17:727-31.
  • Kopcewicz, J. (1970). Influence of estrogens on the flower formation in
  • Cichorium intybus L. Naturwissenschaften, 57:136-137. Marero L.M., Payumo, E.M., Librando, E.C., Lainez, W.N., Gopez, M.D. and Homma, S. (1988). Technology of weaning food formulations prepared from germinated cereals and legumes. J. Food Sci., 53:1391-1395.
  • Martinez-Honduvilla, C.J., Gimenez-Solves, A. and Santos-Ruiz, A. (1976).
  • Biochemical changes in Pinus pinea seeds. III. The effect of growth substances and steroidal hormones on nucleic acids. Rev. Esp. Fisiol., : 169-174. critical role of sucrose synthase for anoxic tolerance of maize roots using a double mutant. Plant Physiol., 116:1323–1331.
  • Simerskŷ, R., Novák, O., Morris, D.A., Pouza,r V. and Strnad, M. (2009).
  • Identification and Quantification of Several Mammalian Steroid Hormones in Plants by UPLC-MS/MS. J Plant Growth Regul., 28:125- Subbarao, K.V., Datta, R. and Sharma, R. (1988). Amylases synthesis in scutellum and aleurone layer of maize seeds. Phytochemistry, 49:657
  • Young, I.J., Knights, B.A. and Hillman, J.R. (1977). Oestradiol and its biosynthesis in Phaseolus vulgaris L. Nature, 267: 429. ****
  • EÜFBED - Fen Bilimleri Enstitüsü Dergisi Cilt-Sayı: 3-1 Yıl: 2010 61-71
  • Tablo 1. Memeli cinsiyet hormonlarının çimlenen fasulye tohumlarının çimlenme yüzdesi, kök-gövde uzunlukları ve α-amilaz enzim aktivitesi üzerine etkileri Çimlenme yüzdesi (%) (cm) (cm) (EU/g) Uygu hormo K on gün gün gün gün gün gün gün gün a b c d c b a b d d c b a b cd d bc ab ab a 60bc 32b 55ab 1.29b 20b a a ab 15 An Aynı sütunda aynı harfler ile gösterilen ortalamalar arasındaki fark istatistiksel olarak önemli değildir (p<0,05).
Year 2010, Volume: 3 Issue: 1, 61 - 71, 11.03.2014

Abstract

References

  • Aisien, A.O. and Stark, J.R. (1983). The development of enzymes during germination and seedling growth in Nigerian sorghum. Staerke. 35:316
  • Barrington, E.J.W. (1979). in: Hormones and Evolution (E.J.W. Barrington, ed.)
  • (pp. VII-XXI). London, Academic Press. Bewley, J.D. and Black M. (1994). Seeds: Physiology of Development and Germination. 2nd ed. (pp 1-31).New York and London, Plenum Press. deMorais, G.A. and Takaki, M. (1998). Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca. Braz. Arch. Biol. Techn., :17-25.
  • Dogra, R. and Kaur, A. (1994). Effect of steroids on some growth and biochemical parameters of Triticum aestivum L. during germination. Crop Res., 8: 611-620.
  • Dogra, R. and Thukral, A. (1991). Effect of steroid and plant hormones on some germination aspects of Triticum aestivum L. In: New trends in plant physiology Physiology (Eds. K. K. Dhir, I. S. Dua and K. S. Chark),
  • (pp. 65-70). New Delhi, Today and Tomorrow's Printers and Publishers. Dohrn, M., Faure, W., Poll, H. and Blotevogel, W. (1926). Tokokinine, Stoff mit sexualhormonartiger Wirkung aus Pflanzenzellen. Med Klin., :1417-1419.
  • Dow, M.A. and Schwintzer, C.R. (1999). Seed germination, seedling emergence, and seed bank ecology of sweet fern (comptonia peregrina (l.) Coult.). Can. J. Bot., 77: 1378-1386.
  • Geuns, J. M. C. (1978) Steroid hormones and plant growth and development. Phytochem. 17, 1–14.
  • Guan, M. and Roddick, J.G. (1988). Comparison of the effects of epibrassinolide and steroidal estrogens on adventitious root-growth and early shoot development in mung bean cuttings. Physiol. Plantarum, 73: 426-431.
  • Guglielminetti, L., Busilacchi, H.A. and Alpi A. (2000). Effect of anoxia on a- amylase induction in maize caryopsis. J. Plant Res., 113:185-92.
  • Heftmann, E. (1975). Steroid hormones in plants. Lloydia, 38:195-209.
  • Helmkamp, G. and Bonner, J. (1952). Some relationships of sterols to plant growth. Plant Physiol., 28:428-436.
  • Janeczko, A. and Skoczowski, A. (2005). Mammalian sex hormones in plants.
  • Folia Histochem. Cyto., 43:71-79. Juliano, B. and Varner, J.E. (1969). Enzymic Degradation of Starch Granules in the Cotyledons of Germinating Peas. Plant Physiol., 44:886-892.
  • Kato-Noguchi, H. and Macias, F.M. (2005). Effects of 6-methoxy-2- benzoxazolinone on the germination and α-amylase activity in lettuce seeds. J. Plant Physiol., 162:1304-1307.
  • Kopcewicz, J. (1969). Influence of estrone on growth and endogenous gibberellin content in dwarf pea. Bull. Acad. Pol. Sci. Cl. II. Ser. Sci. Biol., 17:727-31.
  • Kopcewicz, J. (1970). Influence of estrogens on the flower formation in
  • Cichorium intybus L. Naturwissenschaften, 57:136-137. Marero L.M., Payumo, E.M., Librando, E.C., Lainez, W.N., Gopez, M.D. and Homma, S. (1988). Technology of weaning food formulations prepared from germinated cereals and legumes. J. Food Sci., 53:1391-1395.
  • Martinez-Honduvilla, C.J., Gimenez-Solves, A. and Santos-Ruiz, A. (1976).
  • Biochemical changes in Pinus pinea seeds. III. The effect of growth substances and steroidal hormones on nucleic acids. Rev. Esp. Fisiol., : 169-174. critical role of sucrose synthase for anoxic tolerance of maize roots using a double mutant. Plant Physiol., 116:1323–1331.
  • Simerskŷ, R., Novák, O., Morris, D.A., Pouza,r V. and Strnad, M. (2009).
  • Identification and Quantification of Several Mammalian Steroid Hormones in Plants by UPLC-MS/MS. J Plant Growth Regul., 28:125- Subbarao, K.V., Datta, R. and Sharma, R. (1988). Amylases synthesis in scutellum and aleurone layer of maize seeds. Phytochemistry, 49:657
  • Young, I.J., Knights, B.A. and Hillman, J.R. (1977). Oestradiol and its biosynthesis in Phaseolus vulgaris L. Nature, 267: 429. ****
  • EÜFBED - Fen Bilimleri Enstitüsü Dergisi Cilt-Sayı: 3-1 Yıl: 2010 61-71
  • Tablo 1. Memeli cinsiyet hormonlarının çimlenen fasulye tohumlarının çimlenme yüzdesi, kök-gövde uzunlukları ve α-amilaz enzim aktivitesi üzerine etkileri Çimlenme yüzdesi (%) (cm) (cm) (EU/g) Uygu hormo K on gün gün gün gün gün gün gün gün a b c d c b a b d d c b a b cd d bc ab ab a 60bc 32b 55ab 1.29b 20b a a ab 15 An Aynı sütunda aynı harfler ile gösterilen ortalamalar arasındaki fark istatistiksel olarak önemli değildir (p<0,05).
There are 25 citations in total.

Details

Primary Language Turkish
Journal Section Makaleler
Authors

Serkan Erdal

Mesut Taşkın

Publication Date March 11, 2014
Published in Issue Year 2010 Volume: 3 Issue: 1

Cite

APA Erdal, S., & Taşkın, M. (2014). ÇİMLENEN FASULYE TOHUMLARINDA α–AMİLAZ ENZİM AKTİVİTESİ VE ERKEN BÜYÜME PARAMETRELERİ ÜZERİNE MEMELİ CİNSİYET HORMONLARININ ETKİLERİ. Erzincan University Journal of Science and Technology, 3(1), 61-71.