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Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları ve Kardiyak Troponin I Düzeyleri

Year 2015, Volume: 12 Issue: 3, 163 - 170, 01.10.2015

Halim Akın Vehbi Güneş İlknur Karaca Bekdik

Abstract

 Bu çalışmanın amacı, immünokromatografik test kitleri ile şap hastalığı (FMD) bulunan besi sığırlarında cTn-I’nın belirlenmesi
ve miyokard dejenerasyonlarında söz konusu parametrenin düzeylerini belirlemekti. Bu amaçla akut FMD’li (I. Grup)
ortalama 12.4±0.5 aylık yaşta 69 adet ve klinik olarak sağlıklı (II. Grup) ortalama 10.4±0.6 aylık yaşta 20 adet sığır kullanıldı.
Her iki grupta da kreatin kinaz-MB (CK-MB) ve laktat dehidrogenaz (LDH ) aktiviteleri, cTn-I düzeyleri ve cTn-I varlığı belirlendi.
FMD’li sığırlarda ortalama CK-MB ve LDH aktiviteleri, cTn-I düzeyi sırasıyla ortalama 795.1±70.8 U/L, 1305.9±51.4 U/L
ve 3.6±0.3 ng/ml olarak bulundu. Aynı parametreler II.Grupta sırasıyla ortalama 306±20.5 U/L, 1084.7±43.2 U/L ve 1.1±0.02
ng/ml olduğu belirlendi. Bu parametreler I. Grupta, kontrol grubuna göre istatistiksel olarak yüksek bulundu (p<0.05). Pratik
cTn-I kitlerinin kalp kası dejenerasyonunun tanısında duyarlılık ve seçicilik oranları sırasıyla %100 ve %76.92 olarak bulundu.
FMD’li sığırlardan yalnızca beşinde kardiyak Tn-I testleri pozitif sonuç verdi. Ancak bu pratik testler sağlıklı sığırlardan toplanan
kan örneklerinde negatif idi. Makroskobik bulgular, serum cTn-I düzeyleri ve pozitif cTn-I sonuçları Şap hastalığına bağlı
miyokard dejenerasyonu olan vakalarla uyumlu bulundu. Sonuç olarak, hem kantitatif cTn-I analizleri hem de kalitatif cTn-I
varlığı FMD’li sığırlarda ölüm oranının belirlenmesi için duyarlı ve spesifik bir belirleyici olabileceği ve sığırlarda gelişebilecek
kalp kası hastalıklarını belirlemek için kullanılabileceği gösterilmiştir.

Keywords

CK-MB cTn-I LDH sığır şap hastalığı

References

  • 1. Al Shami SA. Studies on Normal Haematological and Biochemical Parameters of Hassawi cattle Breed in Saudia Arabia. Pak J Biol Sci 2003; 6 (14): 1241-2.
  • 2. Azzazy HME, Christenson RH. Cardiac markers of acute coronary syndrome: Is there a case for point-of-care testing. Clin Biochem 2002; 35(1): 13-27.
  • 3. Bademkiran S, Yokus B, Icen H, Cakir DU, Kurt D. Assesment of Serum Mineral and Certain Biochemical Variables in Self-Sucking Dairy Cows. JAVA 2008; 7 (24): 717-22.
  • 4. Barnett PV, Cox SJ. The role of small ruminants in the epidemiology and transmission of Foot-and-Mouth Disease. Vet J 1999: 158(1): 6-13.
  • 5. Bertsch T, Bleuel H, Aufenanger J, Rebel W. Comparison of cardiac troponin-T and cardiac troponin-I concentrations in peripheral blood during orciprenaline induced tachycardia in rats. Exp Toxicol Pathol 1997; 49 (6): 467-8.
  • 6. Boccara G, Pouzeratte Y, Troncin R, Bonardet A, Boularan AM, Colson P, Mann C. The risk of cardiac injury during laparoscopic fundoplication. Cardiac troponin I and ECG study. Acta Anaesthesiol Scand 2000; 44(4): 398-402.
  • 7. Brandt RR, Filzmaier K, Hanrath P. Circulating cardiac troponin I in acute pericarditis. Am J Cardiol 2001; 87(11): 1326-8.
  • 8. Buczinski S, Bélanger AM. Bovine tricuspid endocarditis as a cause of increased serum concentration of cardiac troponins. Can Vet J 2010; 51(12): 195-7.
  • 9. Buczinski S, Rezakhani A, Boerboom D. Heart disease in cattle: Diagnosis, therapeutic approaches and prognosis. Vet J 2010; 184(3): 258-63.
  • 10. Cardinet GH. Skeletal muscle function. K. Kaneko, eds. In Clinical Biochemistry of Domestic Animals. Fifth Edition. San Diego: Academic Press, 1997a; pp. 410-4.
  • 11. Cardinet GH. Skeletal muscle function. K. Kaneko, eds. In Clinical Biochemistry of Domestic Animals. Fifth Edition. San Diego: Academic Press, 1997b; pp. 424-9.
  • 12. Christenson RH, Apple FS, Morgan DL, Alonsozona GL, Mascotti K, Olson M, McCormack RT, Wians FH Jr, Keffer JH, Duh SH. Cardiac troponin-I measurement with the Access immunoassay system: analytical and clinical performance characteristics. Clin Chem 1998; 44(1): 52-60.
  • 13. Doornenbal H, Tong AKW, Murray NL. Reference Values of Blood Parameters in Beef Cattle of Different Ages and Stages of Lactation. Can J Vet Res 1988; 52(1): 99-105.
  • 14. Gunes V, Atalan G, Citil M, Erdogan HM. Use of cardiac troponin kits for the qualitative determination of myocardial cell damage due to traumatic reticuloperitonitis in cattle. Vet Rec 2008; 162(16): 514-7.
  • 15. Gunes V, Erdogan HM, Citil M, Ozcan K. Assay of cardiac troponins in the diagnosis of myocardial degeneration due to foot-and-mouth disease in a calf. Vet Rec 2005; 156(22): 714-5.
  • 16. Imazio M, Demichelis B, Cecchi E, Belli R, Ghisio A, Bobbio M, Trinchero R. Cardiac troponin I in acute pericarditis. J Am Coll Cardiol 2003; 42(12): 2144-8.
  • 17. Jesty SA, Sweeney RA, Dolente BA, Reef BA. Idiopathic and cardiac tamponade in two cows. J Am Vet Med Assoc 2005; 226(9): 1555-8.
  • 18. Jurlander B, Clemensen P, Wagner GS, Grande P. Very early diagnosis and risk stratification of patients admitted with suspected acute myocardial infarction by the combined evaluation of a single serum value of cardiac troponin-T, myoglobin, and creatine kinase MB. Eur Heart J 2000; 21(5): 382-9.
  • 19. Karapinar T, Dabak DO, Kuloğlu T, Bulut H. High cardiac troponin I plasma concentration in a calf with myocarditis. Can Vet J 2010; 51: 397-9.
  • 20. M-Bardorff M, Hallermayer K, Schro A, Ebert C. Improved troponin T ELISA specific for cardiac troponin T isoform: assay development and analytical and clinical validation. Clin Chem 1999; 43(3): 458-66.
  • 21. Mellanby RJ, Henry JP, Cash R, Ricketts SW, Bexiga R, Truyers I, Mellor DJ. Serum cardiac troponin I concentrations in cattle with cardiac and noncardiac disorders. J Vet Intern Med 2009; 23(4): 926-30.
  • 22. Mellanby RJ, Henry JP, Cash R, Ricketts SW, Dias Bexiga JR, Mellor DJ. Serum cardiac troponin I concentrations in cattle with pericarditis. Vet Rec 2007; 161(13): 454-5.
  • 23. Mezencio JMS, Babcock GD, Kramer E, Brown F. Evidence for the pesistence of Foot-and-Mouth Disease virus in pigs. Vet J 1999; 157(3): 213-7.
  • 24. O’Brien PJ. Cardiac troponin is the most effective translational safety biomarker for myocardial injury in cardiotoxicity. Toxicol 2008; 245(3): 206-18.
  • 25. O’Brien PJ, Dameron GW, Beck ML. Differantial reactivity of cardiac and skeletal muscle from various species in two generations of cardiac troponin-T immunoassays. Res Vet Sci 1998; 65(2): 135-7.
  • 26. O’Brien PJ, Landt Y, Ladenson JH. Differential reactivity of cardiac and skeletal muscle from various species in a cardiac troponin I immunoassay. Clin Chem 1997; 43(12): 2333-8.
  • 27. Oyama MA, Sisson DD. Cardiac troponin-I concentration in dogs with cardiac disease. J Vet Intern Med 2004; 18(6): 831-9.
  • 28. Panteghini M. Present issues in the determination of troponins and other markers of cardiac damage. Clin Biochem 2000; 33(3): 161-6.
  • 29. Reef VB, Mcguirk SM. Diseases of the cardiovascular system. BP Smith. Eds. In Large Animal Internal Medicine. Third Edition. St. Louis: Mosby, 2002; pp. 443-78.
  • 30. Smith BP. Large Animal Internal Medicine. Third Edition. Philadelphia/USA: Mosby-year book, 2002; p. 718.
  • 31. Tunca R, Erdogan HM, Sonmez M, Citil M, Devrim AK, Erginsoy S, Uzlu E. Evaluation of cardiac troponin I and inducible nitric oxide synthase expressions in lambs with White Muscle Disease. Turk J Vet Anim Sci 2009; 33(1): 53-9.
  • 32. Tunca R, Sozmen M, Erdogan HM, Citil M, Uzlu E, Ozen H, Gokce E. Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease. J Vet Diagn Invest 2008; 20(5): 598-605.
  • 33. Undhad VV, Fefar DT, Jivani BM, Gupta H, Ghodasara DJ, Joshi BP, Prajapati KS. Cardiac troponin: an emerging cardiac biomarker in animal health. Vet World 2012; 5(8): 508-11.
  • 34. Utlu N, Yucel O, Kaya N. Farklı Irk Sığırlarda bazı Serum Enzim Aktiviteleri. Kafkas Univ Vet Fak Derg 2005; 11(1): 39-41.
  • 35. Van Vleet JF, Ferrans JV. Myocardial Disease of Animals. Am J Pathol 1986; 124(1): 98-178.
  • 36. Varga A, Angelos JA, Graham TW, Chigerwe M. Preliminary Investigation of Cardiac Troponin I Concentration in Cows with Common Production Diseases. J Vet Intern Med 2013; 27(6): 1613-21.
  • 37. Varga A, Schober KE, Walker WL, Lakritz J, Michael RD. Validation of a commercially available immunoassay for the measurement of bovine cardiac troponin I. J Vet Intern Med 2009; 23(2): 359-65.
  • 38. Yang PC, Chu RM, Chung WB, Sung HT. Epidemiological characteristic and financial costs of the 1997 foot and-mouth disease epidemic in Taiwan. Vet Rec 1999; 145(25): 731-4.

Practice Cardiac Troponin I Kit Expressions and Concentrations of Cardiac Troponin I in Beef Cattle with Foot and Mouth Disease*

Year 2015, Volume: 12 Issue: 3, 163 - 170, 01.10.2015

Halim Akın Vehbi Güneş İlknur Karaca Bekdik

Abstract

 The aim of this study was to determine the expression of cTn-I with immunochromatographic strip kits, the concentrations of serum cTn-I in myocardial degeneration due to foot and mouth disease (FMD) with these parameters in beef cattle. For this purpose sixtynine cattle with acute FMD (Group I), mean age 12.4±0.5 months old and 20 clinically healthy cattle (Group II), mean age 10.4±0.6 months old were used. Creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) activities, cTn-I concentrations and expression of cTn-I were determined in both groups. Mean CK-MB, LDH activities and cTn-I concentration were found as 795.1±70.8 U/L, 1305.9±51.4 U/L and 3.6±0.3 ng/ml respectively in cattle with FMD. The mean of the same parameters were 306±20.5 U/L, 1084.7±43.2 U/L and 1.1±0.02 ng/ml in Group II, respectively. These parameters in Group I were statistically higher than those of control group (p<0.05). The sensitivity and specificity of cTn-I kits for the diagnosis myocardial degeneration were 100% and 76.9%, respectively. Cardiac Tn-I tests were positive in the only five cattle with FMD, but these tests were negative in blood samples collected from healthy cattle. Macroscobic findings, serum cTn-I concentrations and positive cTn-I results were correlated with FMD cases suffering from myocardial degeneration. In conclusion, both quantitative cTn-I analysess and qualitative cTn-I expressions may be sensitive and specific marker for the determination of mortality rate in cattle with FMD, and can be used to predict the future cardiac disorders in cattle.

Keywords

Cattle CK-MB cTn-I FMD LDH

References

  • 1. Al Shami SA. Studies on Normal Haematological and Biochemical Parameters of Hassawi cattle Breed in Saudia Arabia. Pak J Biol Sci 2003; 6 (14): 1241-2.
  • 2. Azzazy HME, Christenson RH. Cardiac markers of acute coronary syndrome: Is there a case for point-of-care testing. Clin Biochem 2002; 35(1): 13-27.
  • 3. Bademkiran S, Yokus B, Icen H, Cakir DU, Kurt D. Assesment of Serum Mineral and Certain Biochemical Variables in Self-Sucking Dairy Cows. JAVA 2008; 7 (24): 717-22.
  • 4. Barnett PV, Cox SJ. The role of small ruminants in the epidemiology and transmission of Foot-and-Mouth Disease. Vet J 1999: 158(1): 6-13.
  • 5. Bertsch T, Bleuel H, Aufenanger J, Rebel W. Comparison of cardiac troponin-T and cardiac troponin-I concentrations in peripheral blood during orciprenaline induced tachycardia in rats. Exp Toxicol Pathol 1997; 49 (6): 467-8.
  • 6. Boccara G, Pouzeratte Y, Troncin R, Bonardet A, Boularan AM, Colson P, Mann C. The risk of cardiac injury during laparoscopic fundoplication. Cardiac troponin I and ECG study. Acta Anaesthesiol Scand 2000; 44(4): 398-402.
  • 7. Brandt RR, Filzmaier K, Hanrath P. Circulating cardiac troponin I in acute pericarditis. Am J Cardiol 2001; 87(11): 1326-8.
  • 8. Buczinski S, Bélanger AM. Bovine tricuspid endocarditis as a cause of increased serum concentration of cardiac troponins. Can Vet J 2010; 51(12): 195-7.
  • 9. Buczinski S, Rezakhani A, Boerboom D. Heart disease in cattle: Diagnosis, therapeutic approaches and prognosis. Vet J 2010; 184(3): 258-63.
  • 10. Cardinet GH. Skeletal muscle function. K. Kaneko, eds. In Clinical Biochemistry of Domestic Animals. Fifth Edition. San Diego: Academic Press, 1997a; pp. 410-4.
  • 11. Cardinet GH. Skeletal muscle function. K. Kaneko, eds. In Clinical Biochemistry of Domestic Animals. Fifth Edition. San Diego: Academic Press, 1997b; pp. 424-9.
  • 12. Christenson RH, Apple FS, Morgan DL, Alonsozona GL, Mascotti K, Olson M, McCormack RT, Wians FH Jr, Keffer JH, Duh SH. Cardiac troponin-I measurement with the Access immunoassay system: analytical and clinical performance characteristics. Clin Chem 1998; 44(1): 52-60.
  • 13. Doornenbal H, Tong AKW, Murray NL. Reference Values of Blood Parameters in Beef Cattle of Different Ages and Stages of Lactation. Can J Vet Res 1988; 52(1): 99-105.
  • 14. Gunes V, Atalan G, Citil M, Erdogan HM. Use of cardiac troponin kits for the qualitative determination of myocardial cell damage due to traumatic reticuloperitonitis in cattle. Vet Rec 2008; 162(16): 514-7.
  • 15. Gunes V, Erdogan HM, Citil M, Ozcan K. Assay of cardiac troponins in the diagnosis of myocardial degeneration due to foot-and-mouth disease in a calf. Vet Rec 2005; 156(22): 714-5.
  • 16. Imazio M, Demichelis B, Cecchi E, Belli R, Ghisio A, Bobbio M, Trinchero R. Cardiac troponin I in acute pericarditis. J Am Coll Cardiol 2003; 42(12): 2144-8.
  • 17. Jesty SA, Sweeney RA, Dolente BA, Reef BA. Idiopathic and cardiac tamponade in two cows. J Am Vet Med Assoc 2005; 226(9): 1555-8.
  • 18. Jurlander B, Clemensen P, Wagner GS, Grande P. Very early diagnosis and risk stratification of patients admitted with suspected acute myocardial infarction by the combined evaluation of a single serum value of cardiac troponin-T, myoglobin, and creatine kinase MB. Eur Heart J 2000; 21(5): 382-9.
  • 19. Karapinar T, Dabak DO, Kuloğlu T, Bulut H. High cardiac troponin I plasma concentration in a calf with myocarditis. Can Vet J 2010; 51: 397-9.
  • 20. M-Bardorff M, Hallermayer K, Schro A, Ebert C. Improved troponin T ELISA specific for cardiac troponin T isoform: assay development and analytical and clinical validation. Clin Chem 1999; 43(3): 458-66.
  • 21. Mellanby RJ, Henry JP, Cash R, Ricketts SW, Bexiga R, Truyers I, Mellor DJ. Serum cardiac troponin I concentrations in cattle with cardiac and noncardiac disorders. J Vet Intern Med 2009; 23(4): 926-30.
  • 22. Mellanby RJ, Henry JP, Cash R, Ricketts SW, Dias Bexiga JR, Mellor DJ. Serum cardiac troponin I concentrations in cattle with pericarditis. Vet Rec 2007; 161(13): 454-5.
  • 23. Mezencio JMS, Babcock GD, Kramer E, Brown F. Evidence for the pesistence of Foot-and-Mouth Disease virus in pigs. Vet J 1999; 157(3): 213-7.
  • 24. O’Brien PJ. Cardiac troponin is the most effective translational safety biomarker for myocardial injury in cardiotoxicity. Toxicol 2008; 245(3): 206-18.
  • 25. O’Brien PJ, Dameron GW, Beck ML. Differantial reactivity of cardiac and skeletal muscle from various species in two generations of cardiac troponin-T immunoassays. Res Vet Sci 1998; 65(2): 135-7.
  • 26. O’Brien PJ, Landt Y, Ladenson JH. Differential reactivity of cardiac and skeletal muscle from various species in a cardiac troponin I immunoassay. Clin Chem 1997; 43(12): 2333-8.
  • 27. Oyama MA, Sisson DD. Cardiac troponin-I concentration in dogs with cardiac disease. J Vet Intern Med 2004; 18(6): 831-9.
  • 28. Panteghini M. Present issues in the determination of troponins and other markers of cardiac damage. Clin Biochem 2000; 33(3): 161-6.
  • 29. Reef VB, Mcguirk SM. Diseases of the cardiovascular system. BP Smith. Eds. In Large Animal Internal Medicine. Third Edition. St. Louis: Mosby, 2002; pp. 443-78.
  • 30. Smith BP. Large Animal Internal Medicine. Third Edition. Philadelphia/USA: Mosby-year book, 2002; p. 718.
  • 31. Tunca R, Erdogan HM, Sonmez M, Citil M, Devrim AK, Erginsoy S, Uzlu E. Evaluation of cardiac troponin I and inducible nitric oxide synthase expressions in lambs with White Muscle Disease. Turk J Vet Anim Sci 2009; 33(1): 53-9.
  • 32. Tunca R, Sozmen M, Erdogan HM, Citil M, Uzlu E, Ozen H, Gokce E. Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease. J Vet Diagn Invest 2008; 20(5): 598-605.
  • 33. Undhad VV, Fefar DT, Jivani BM, Gupta H, Ghodasara DJ, Joshi BP, Prajapati KS. Cardiac troponin: an emerging cardiac biomarker in animal health. Vet World 2012; 5(8): 508-11.
  • 34. Utlu N, Yucel O, Kaya N. Farklı Irk Sığırlarda bazı Serum Enzim Aktiviteleri. Kafkas Univ Vet Fak Derg 2005; 11(1): 39-41.
  • 35. Van Vleet JF, Ferrans JV. Myocardial Disease of Animals. Am J Pathol 1986; 124(1): 98-178.
  • 36. Varga A, Angelos JA, Graham TW, Chigerwe M. Preliminary Investigation of Cardiac Troponin I Concentration in Cows with Common Production Diseases. J Vet Intern Med 2013; 27(6): 1613-21.
  • 37. Varga A, Schober KE, Walker WL, Lakritz J, Michael RD. Validation of a commercially available immunoassay for the measurement of bovine cardiac troponin I. J Vet Intern Med 2009; 23(2): 359-65.
  • 38. Yang PC, Chu RM, Chung WB, Sung HT. Epidemiological characteristic and financial costs of the 1997 foot and-mouth disease epidemic in Taiwan. Vet Rec 1999; 145(25): 731-4.
There are 38 citations in total.

Details

Journal Section Articles
Authors

Halim Akın This is me

Vehbi Güneş

İlknur Karaca Bekdik

Publication Date October 1, 2015
Submission Date December 7, 2016
Acceptance Date January 6, 2015
Published in Issue Year 2015 Volume: 12 Issue: 3

Cite

APA Akın, H., Güneş, V., & Bekdik, İ. K. (2015). Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları ve Kardiyak Troponin I Düzeyleri. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 12(3), 163-170.
AMA Akın H, Güneş V, Bekdik İK. Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları ve Kardiyak Troponin I Düzeyleri. Erciyes Üniv Vet Fak Derg. October 2015;12(3):163-170.
Chicago Akın, Halim, Vehbi Güneş, and İlknur Karaca Bekdik. “Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları Ve Kardiyak Troponin I Düzeyleri”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 12, no. 3 (October 2015): 163-70.
EndNote Akın H, Güneş V, Bekdik İK (October 1, 2015) Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları ve Kardiyak Troponin I Düzeyleri. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 12 3 163–170.
IEEE H. Akın, V. Güneş, and İ. K. Bekdik, “Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları ve Kardiyak Troponin I Düzeyleri”, Erciyes Üniv Vet Fak Derg, vol. 12, no. 3, pp. 163–170, 2015.
ISNAD Akın, Halim et al. “Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları Ve Kardiyak Troponin I Düzeyleri”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 12/3 (October 2015), 163-170.
JAMA Akın H, Güneş V, Bekdik İK. Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları ve Kardiyak Troponin I Düzeyleri. Erciyes Üniv Vet Fak Derg. 2015;12:163–170.
MLA Akın, Halim et al. “Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları Ve Kardiyak Troponin I Düzeyleri”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol. 12, no. 3, 2015, pp. 163-70.
Vancouver Akın H, Güneş V, Bekdik İK. Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları ve Kardiyak Troponin I Düzeyleri. Erciyes Üniv Vet Fak Derg. 2015;12(3):163-70.
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