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Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide

Year 2022, Volume: 49 Issue: 1, 36 - 44, 11.03.2022
https://doi.org/10.5798/dicletip.1086124

Abstract

References

  • 1.Cimen B, Cimen L, Cetin I, Cetin A. Alpha-lipoic acidalleviates lipopolysaccharide-induced liver damagein rats via antioxidant effect. Dicle Med J. 2019; 46:125-132.
  • 2.Coskun A, Sen I. Acute phase response and clinicalchanges in calves with lipopolysaccharide inducedendotoxemia. Eurasian J Vet Sci. 2012; 28: 21-26.
  • 3.Aly HA, El-Beshbishy HA, Banjar ZM.Mitochondrial dysfunction induced impairment ofspermatogenesis in LPS-treated rats: modulatoryrole of lycopene. Eur J Pharmacol. 2012; 677: 31-8.
  • 4.Yavaş MC, Akpolat V, Deveci E, et al. Determiningthe effect of an electromagnetic field generated by ahigh voltage power line on rat spermatogonia cells.Dicle Med J. 2018: 45: 447-461.

Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide

Year 2022, Volume: 49 Issue: 1, 36 - 44, 11.03.2022
https://doi.org/10.5798/dicletip.1086124

Abstract

Objective: It was aimed to investigate the antigenotoxic effect of Ganoderma lucidum against the damage caused by lipopolysaccharide (LPS) in rat bone marrow cells by micronucleus test and comet analysis.
Method: In the experiment, 28 Wistar albino female rats were used and 4 groups were formed. Only physiological saline was applied to the control group. A single dose of 7.5 mg/kg LPS was administered intraperitoneally to the LPS group. LPS+ Ganoderma lucidum (GL): Ganoderma lucidum was given by gavage at a dose of 50 mg/kg per day for one week. Then, on the 8th day, LPS was applied intraperitoneally. Ganoderma lucidum group: GL was given by gavage at 50 mg/kg per day for 1 week. After 24 hours, the rats were euthanized by cervical dislocation under anesthesia. Analyzes were performed for percentage of micronucleated polychromatic erythrocytes (MNPCE) and Polychromatic erythrocytes/Normochromatic erythrocytes (PCE/NCE) ratio and DNA damage in rat femur bone marrow cells.
Results: When the percentage of micronucleus and PCE/NCE ratio were examined, no statistical significance was observed when the groups were compared both with the control and among themselves. Compared to the control group, it was observed that the comet parameters of the experimental groups (excluding the head DNA) increased significantly. Length tail (L tail), tail DNA, olive tail moment (OTM) values were significantly different both compared to the control and among themselves. While there was a significant difference between LPS+GL group and LPS and GL groups in Tail Moment (TM) value and Length comet (L comet) value, no difference was observed between LPS and GL groups.
Conclusion: When all of the findings is considered, Ganoderma lucidum cannot be classified as a genotoxic agent. We can say, however, that it reverses the genotoxic damage in issue.

References

  • 1.Cimen B, Cimen L, Cetin I, Cetin A. Alpha-lipoic acidalleviates lipopolysaccharide-induced liver damagein rats via antioxidant effect. Dicle Med J. 2019; 46:125-132.
  • 2.Coskun A, Sen I. Acute phase response and clinicalchanges in calves with lipopolysaccharide inducedendotoxemia. Eurasian J Vet Sci. 2012; 28: 21-26.
  • 3.Aly HA, El-Beshbishy HA, Banjar ZM.Mitochondrial dysfunction induced impairment ofspermatogenesis in LPS-treated rats: modulatoryrole of lycopene. Eur J Pharmacol. 2012; 677: 31-8.
  • 4.Yavaş MC, Akpolat V, Deveci E, et al. Determiningthe effect of an electromagnetic field generated by ahigh voltage power line on rat spermatogonia cells.Dicle Med J. 2018: 45: 447-461.
There are 4 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Original Articles
Authors

Münevver Baran This is me

Nazmiye Bitgen This is me

Kübra Tuğçe Kalkan This is me

Özge Göktepe This is me

Salih Varol

Arzu Yay This is me

Publication Date March 11, 2022
Submission Date November 11, 2021
Published in Issue Year 2022 Volume: 49 Issue: 1

Cite

APA Baran, M., Bitgen, N., Kalkan, K. T., Göktepe, Ö., et al. (2022). Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. Dicle Tıp Dergisi, 49(1), 36-44. https://doi.org/10.5798/dicletip.1086124
AMA Baran M, Bitgen N, Kalkan KT, Göktepe Ö, Varol S, Yay A. Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. diclemedj. March 2022;49(1):36-44. doi:10.5798/dicletip.1086124
Chicago Baran, Münevver, Nazmiye Bitgen, Kübra Tuğçe Kalkan, Özge Göktepe, Salih Varol, and Arzu Yay. “Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide”. Dicle Tıp Dergisi 49, no. 1 (March 2022): 36-44. https://doi.org/10.5798/dicletip.1086124.
EndNote Baran M, Bitgen N, Kalkan KT, Göktepe Ö, Varol S, Yay A (March 1, 2022) Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. Dicle Tıp Dergisi 49 1 36–44.
IEEE M. Baran, N. Bitgen, K. T. Kalkan, Ö. Göktepe, S. Varol, and A. Yay, “Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide”, diclemedj, vol. 49, no. 1, pp. 36–44, 2022, doi: 10.5798/dicletip.1086124.
ISNAD Baran, Münevver et al. “Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide”. Dicle Tıp Dergisi 49/1 (March 2022), 36-44. https://doi.org/10.5798/dicletip.1086124.
JAMA Baran M, Bitgen N, Kalkan KT, Göktepe Ö, Varol S, Yay A. Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. diclemedj. 2022;49:36–44.
MLA Baran, Münevver et al. “Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide”. Dicle Tıp Dergisi, vol. 49, no. 1, 2022, pp. 36-44, doi:10.5798/dicletip.1086124.
Vancouver Baran M, Bitgen N, Kalkan KT, Göktepe Ö, Varol S, Yay A. Evaluation of Genotoxic and Cytotoxic Activity of Ganoderma Lucidum In Rat Bone Marrow Induced By Lipopolysaccharide. diclemedj. 2022;49(1):36-44.