Research Article
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An investigation of Pseudomonas marincola to determine Pb(II) biosensor potential

Year 2023, Volume: 32 Issue: 1, 24 - 35, 03.06.2023

Cihan Bostancı Hasan Nazır Gönül Dönmez

https://doi.org/10.53447/communc.1184290

Abstract

Biosensors are strong alternatives to conventional analytical techniques such as HPLC and spectroscopic techniques for water quality and heavy metal detection. Heavy metal contaminated waters can monitor by microbial biosensors efficiently. For this purpose, newly isolated Pseudomonas sp. is used to develop a highly sensitive low-cost microbial biosensor for water quality monitoring. The objective of the study is the invention of new high sensitive low-cost microbial biosensors to determine heavy metals in aqueous solutions and optimise the working conditions Pseudomonas marincola cells were embedded onto the Screen Printed Electrode (SPE) carbon surface and dried for 30 minutes at laminar flow cabinet. Developed microbial sensors were immersed into the Pb(II) solution for electrochemical analysis. After the exposure time, cyclic voltammetry (CV) and differential pulse voltammetry (DPV) analyses were carried out. The study shows that the sensor was found in a linear range between 1x10−8 M and 8x10−8 M, with the lowest detection limit 10−9 M. The optimum pre-concentration time and scan rate were measured as 10 minutes and 10 mV/s, respectively. The results support that the new isolated Pseudomonas sp. has significant potential to determine the trace amount of lead in aqueous solutions.

Keywords

Pseudomonas marincola biosensor lead heavy metal

Supporting Institution

Ankara University

Project Number

19L0430010

Thanks

C. Bostancı was supported by funding from the Scientific and Technological Research Council of Turkey (TUBITAK-BIDEB 2211-C), Council of Higher Education (YOK-100/2000), American Society for Microbiology and Ankara University (Project Number: 19L0430010).

References

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  • Halliwell, B., Gutteridge, J.M.C., The importance of free radicals and catalytic metal ions in human diseases. Molecular Aspects of Medicine, 8(2) (1985), 89-193. https://doi.org/10.1016/0098-2997(85)90001-9.
  • Laidlaw, M.A.S., Gordon, C., Ball, A.S., Preliminary assessment of surface soil lead concentrations in Melbourne, Australia. Environmental Geochemistry and Health, 40 (2018), 637–650. https://doi.org/10.1007/s10653-017-0010-y.
  • Tsekenis, G., Filippidou, M.K., Chatzipetrou, M., Tsouti, V., Zergioti, I., Chatzandroulis, S., Heavy metal ion detection using a capacitive micromechanical biosensor array for environmental monitoring. Sensors and Actuators B: Chemical, 208 (2015), 628–635. https://doi.org/10.1016/J.SNB.2014.10.093.
  • Chakrapani, G., Mahanta, P., Murty, D.S., Gomathy, B., Preconcentration of traces of gold, silver and palladium on activated carbon and its determination in geological samples by flame AAS after wet ashing. Talanta, 53(6) (2001), 1139–1147. https://doi.org/10.1016/S0039-9140(00)00601-9.
  • Liang, P., Cao, J., Liu, R., Liu, Y., Determination of trace rare earth elements by inductively coupled plasma optical emission spectrometry after preconcentration with immobilized nanometer titanium dioxide. Microchimica Acta, 159(1) (2007), 35–40. https://doi.org/10.1007/s00604-006-0708-5.
  • Pelossof, G., Tel-Vered, R., Willner, I., Amplified Surface Plasmon Resonance and Electrochemical Detection of Pb2+ Ions Using the Pb2+-Dependent DNAzyme and Hemin/G-Quadruplex as a label. Analytical Chemistry, 84(8) (2012), 3703–3709. https://doi.org/10.1021/ac3002269.
  • Duffy, E., Morrin, A., Endogenous and microbial volatile organic compounds in cutaneous health and disease. TrAC - Trends in Analytical Chemistry, 111 (2019), 163-172. https://doi.org/10.1016/j.trac.2018.12.012.
  • De Guzman, K., Al-Kharusi, G., Levingstone, T., Morrin, A., Robust epidermal tattoo electrode platform for skin physiology monitoring. Analytical Methods, 11 (2019) 1460-1468. https://doi.org/10.1039/c8ay02678e.
  • Yüce, M., Nazir, H., Dönmez, G., A voltammetric Rhodotorula mucilaginosa modified microbial biosensor for Cu(II) determination. Bioelectrochemistry, 79(1) (2010), 66-70. https://doi.org/10.1016/j.bioelechem.2009.11.003.
  • Yüce, M., Nazir, H., Dönmez, G., An advanced investigation on a new algal sensor determining Pb(II) ions from aqueous media. Biosensors and Bioelectronics, 22(2) (2010), 321-326. https://doi.org/10.1016/j.bios.2010.08.022.
  • Woolever, C.A., Dewald, H.D., Differential pulse anodic stripping voltammetry of barium and lead in gunshot residues. Forensic Science International, 117 (2001), 185-190. https://doi.org/10.1016/S0379-0738(00)00402-3.
  • Akyilmaz, E., Dinçkaya, E., An amperometric microbial biosensor development based on Candida tropicalis yeast cells for sensitive determination of ethanol. Biosensors and Bioelectronics, 20(7) (2005), 1263–1269. https://doi.org/10.1016/j.bios.2004.04.010. Akyilmaz, E., Yaşa, I., Dinçkaya, E., Whole cell immobilized amperometric biosensor based on Saccharomyces cerevisiae for selective determination of vitamin B1 (thiamine). Analytical Biochemistry, 354 (1) (2006), 78–84. https://doi.org/10.1016/j.ab.2006.04.019.
  • Puy-Llovera, J., Pérez-Ràfols, C., Serrano, N., Díaz-Cruz, J.M., Ariño, C., Esteban, M., Selenocystine modified screen-printed electrode as an alternative sensor for the voltammetric determination of metal ions. Talanta, 175 (2017), 501-506. https://doi.org/10.1016/j.talanta.2017.07.089.
  • Aragay, G., Merkoçi, A., Nanomaterials application in electrochemical detection of heavy metals. Electrochimica Acta, 84 (2012), 49-61. https://doi.org/10.1016/j.electacta.2012.04.044.
  • Pérez-Ràfols, C., Serrano, N., Díaz-Cruz, J.M., Ariño, C., Esteban, M., Penicillamine-modified sensor for the voltammetric determination of Cd(II) and Pb(II) ions in natural samples. Talanta, 114 (2015), 569-573. https://doi.org/10.1016/j.talanta.2015.06.083.
  • Pérez-Ràfols, C., Serrano, N., Díaz-Cruz, J.M., Ariño, C., Esteban, M., A chemically-bound glutathione sensor bioinspired by the defense of organisms against heavy metal contamination: Optimization of the immobilization conditions. Chemosensors, 5(2) (2017), 12. https://doi.org/10.3390/chemosensors5020012.
  • Serrano, N., González-Calabuig, A., Del Valle, M., Crown ether-modified electrodes for the simultaneous stripping voltammetric determination of Cd(II), Pb(II) and Cu(II). Talanta, 138 (2015), 130-137. https://doi.org/10.1016/j.talanta.2015.01.044.
  • Yuan, W., Kuang, J., Yu, M., Huang, Z., Zou, Z., Zhu, L., Facile preparation of MoS2@Kaolin composite by one-step hydrothermal method for efficient removal of Pb(II). Journal of Hazardous Materials, 405 (2020), 124261. https://doi.org/10.1016/j.jhazmat.2020.124261.
  • Bilal, M., Iqbal, H.M.N., Microbial-derived biosensors for monitoring environmental contaminants: Recent advances and future outlook. Process Safety and Environmental Protection, 124 (2019), 8-17. https://doi.org/10.1016/j.psep.2019.01.032.
  • Van Der Meer, J.R., Tropel, D., Jaspers, M., Illuminating the detection chain of bacterial bioreporters. Environmental Microbiology, 6(10) (2004), 1005-1020. https://doi.org/10.1111/j.1462-2920.2004.00655.x.

Year 2023, Volume: 32 Issue: 1, 24 - 35, 03.06.2023

Cihan Bostancı Hasan Nazır Gönül Dönmez

https://doi.org/10.53447/communc.1184290

Abstract

Project Number

19L0430010

References

  • Romanenko, L.A., Uchino, M., Tebo, B.M., Tanaka, N., Frolova, G.M., Mikhailov, V.V., Pseudomonas marincola sp. nov., isolated from marine environments. International Journal of Systematic and Evolutionary Microbiology, 58 (3) (2008), 706–710. https://doi.org/10.1099/ijs.0.65406-0.
  • Khudur, L.S., Gleeson, D.B., Ryan, M.H., Shahsavari, E., Haleyur, H., Nugegoda, d., Ball, A.S., Implications of co-contamination with aged heavy metals and total petroleum hydrocarbons on natural attenuation and ecotoxicity in Australian soils. Environmental Pollution, 243 (2018), 94-102. https://doi.org/10.1016/j.envpol.2018.08.040.
  • Halliwell, B., Gutteridge, J.M.C., The importance of free radicals and catalytic metal ions in human diseases. Molecular Aspects of Medicine, 8(2) (1985), 89-193. https://doi.org/10.1016/0098-2997(85)90001-9.
  • Laidlaw, M.A.S., Gordon, C., Ball, A.S., Preliminary assessment of surface soil lead concentrations in Melbourne, Australia. Environmental Geochemistry and Health, 40 (2018), 637–650. https://doi.org/10.1007/s10653-017-0010-y.
  • Tsekenis, G., Filippidou, M.K., Chatzipetrou, M., Tsouti, V., Zergioti, I., Chatzandroulis, S., Heavy metal ion detection using a capacitive micromechanical biosensor array for environmental monitoring. Sensors and Actuators B: Chemical, 208 (2015), 628–635. https://doi.org/10.1016/J.SNB.2014.10.093.
  • Chakrapani, G., Mahanta, P., Murty, D.S., Gomathy, B., Preconcentration of traces of gold, silver and palladium on activated carbon and its determination in geological samples by flame AAS after wet ashing. Talanta, 53(6) (2001), 1139–1147. https://doi.org/10.1016/S0039-9140(00)00601-9.
  • Liang, P., Cao, J., Liu, R., Liu, Y., Determination of trace rare earth elements by inductively coupled plasma optical emission spectrometry after preconcentration with immobilized nanometer titanium dioxide. Microchimica Acta, 159(1) (2007), 35–40. https://doi.org/10.1007/s00604-006-0708-5.
  • Pelossof, G., Tel-Vered, R., Willner, I., Amplified Surface Plasmon Resonance and Electrochemical Detection of Pb2+ Ions Using the Pb2+-Dependent DNAzyme and Hemin/G-Quadruplex as a label. Analytical Chemistry, 84(8) (2012), 3703–3709. https://doi.org/10.1021/ac3002269.
  • Duffy, E., Morrin, A., Endogenous and microbial volatile organic compounds in cutaneous health and disease. TrAC - Trends in Analytical Chemistry, 111 (2019), 163-172. https://doi.org/10.1016/j.trac.2018.12.012.
  • De Guzman, K., Al-Kharusi, G., Levingstone, T., Morrin, A., Robust epidermal tattoo electrode platform for skin physiology monitoring. Analytical Methods, 11 (2019) 1460-1468. https://doi.org/10.1039/c8ay02678e.
  • Yüce, M., Nazir, H., Dönmez, G., A voltammetric Rhodotorula mucilaginosa modified microbial biosensor for Cu(II) determination. Bioelectrochemistry, 79(1) (2010), 66-70. https://doi.org/10.1016/j.bioelechem.2009.11.003.
  • Yüce, M., Nazir, H., Dönmez, G., An advanced investigation on a new algal sensor determining Pb(II) ions from aqueous media. Biosensors and Bioelectronics, 22(2) (2010), 321-326. https://doi.org/10.1016/j.bios.2010.08.022.
  • Woolever, C.A., Dewald, H.D., Differential pulse anodic stripping voltammetry of barium and lead in gunshot residues. Forensic Science International, 117 (2001), 185-190. https://doi.org/10.1016/S0379-0738(00)00402-3.
  • Akyilmaz, E., Dinçkaya, E., An amperometric microbial biosensor development based on Candida tropicalis yeast cells for sensitive determination of ethanol. Biosensors and Bioelectronics, 20(7) (2005), 1263–1269. https://doi.org/10.1016/j.bios.2004.04.010. Akyilmaz, E., Yaşa, I., Dinçkaya, E., Whole cell immobilized amperometric biosensor based on Saccharomyces cerevisiae for selective determination of vitamin B1 (thiamine). Analytical Biochemistry, 354 (1) (2006), 78–84. https://doi.org/10.1016/j.ab.2006.04.019.
  • Puy-Llovera, J., Pérez-Ràfols, C., Serrano, N., Díaz-Cruz, J.M., Ariño, C., Esteban, M., Selenocystine modified screen-printed electrode as an alternative sensor for the voltammetric determination of metal ions. Talanta, 175 (2017), 501-506. https://doi.org/10.1016/j.talanta.2017.07.089.
  • Aragay, G., Merkoçi, A., Nanomaterials application in electrochemical detection of heavy metals. Electrochimica Acta, 84 (2012), 49-61. https://doi.org/10.1016/j.electacta.2012.04.044.
  • Pérez-Ràfols, C., Serrano, N., Díaz-Cruz, J.M., Ariño, C., Esteban, M., Penicillamine-modified sensor for the voltammetric determination of Cd(II) and Pb(II) ions in natural samples. Talanta, 114 (2015), 569-573. https://doi.org/10.1016/j.talanta.2015.06.083.
  • Pérez-Ràfols, C., Serrano, N., Díaz-Cruz, J.M., Ariño, C., Esteban, M., A chemically-bound glutathione sensor bioinspired by the defense of organisms against heavy metal contamination: Optimization of the immobilization conditions. Chemosensors, 5(2) (2017), 12. https://doi.org/10.3390/chemosensors5020012.
  • Serrano, N., González-Calabuig, A., Del Valle, M., Crown ether-modified electrodes for the simultaneous stripping voltammetric determination of Cd(II), Pb(II) and Cu(II). Talanta, 138 (2015), 130-137. https://doi.org/10.1016/j.talanta.2015.01.044.
  • Yuan, W., Kuang, J., Yu, M., Huang, Z., Zou, Z., Zhu, L., Facile preparation of MoS2@Kaolin composite by one-step hydrothermal method for efficient removal of Pb(II). Journal of Hazardous Materials, 405 (2020), 124261. https://doi.org/10.1016/j.jhazmat.2020.124261.
  • Bilal, M., Iqbal, H.M.N., Microbial-derived biosensors for monitoring environmental contaminants: Recent advances and future outlook. Process Safety and Environmental Protection, 124 (2019), 8-17. https://doi.org/10.1016/j.psep.2019.01.032.
  • Van Der Meer, J.R., Tropel, D., Jaspers, M., Illuminating the detection chain of bacterial bioreporters. Environmental Microbiology, 6(10) (2004), 1005-1020. https://doi.org/10.1111/j.1462-2920.2004.00655.x.
There are 22 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Cihan Bostancı 0000-0001-7846-9181

Hasan Nazır 0000-0002-8423-751X

Gönül Dönmez 0000-0001-7972-5570

Project Number 19L0430010
Publication Date June 3, 2023
Acceptance Date January 26, 2023
Published in Issue Year 2023 Volume: 32 Issue: 1

Cite

Communications Faculty of Sciences University of Ankara Series C-Biology.

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