Research Article
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Year 2018, Volume: 14 Issue: 4, 405 - 412, 28.12.2018
https://doi.org/10.18466/cbayarfbe.426444

Abstract

References

  • 1. Ravindran, B, Gupta, S.K, Cho, W.M, Kim, J.K, Lee, S.R, Jeong, K.H, Lee, D.J, Choi, H-C, Microalgae Potential and Multiple Roles-Current Progress and Future Prospects-An Overview, Sustainability, 2016, 8(1215), 1-16.
  • 2. Demirel, Z, Demirkaya, C, Imamoglu, E, Dalay, M.C, Diatom cultivation and lipid productivity for non-cryopreserved and cryopreserved cells, Agronomy Research, 2016, 14(4), 1266-1273.
  • 3. Nakanishi, K, Deuchi, K, Kuwano, K, Cryopreservation of four valuable strains of microalgae, including viability and characteristics during 15 years of cryostorage, Journal of Applied Phycology, 2012, 24(6), 1381-1385.
  • 4. Kumari, N, Gupta, M.K, Singh, R.K, Open encapsulation-vitrification for cryopreservation of algae, Cryobiology, 2016, 73(2), 232-239. 5. Kaur, R, Pramanik, K, Sarangi, S.K, Cryopreservation-induced stress on long-term preserved articular cartilage, ISRN Tissue Engineering, 2013, 2013, 1-10.
  • 6. Day, J.G, Fleck, R.A, Benson, E.E, Cryopreservation-recalcitrance in microalgae: novel approaches to identify and avoid cryo-injury, Journal of Applied Phycology, 2000, 12(3-5), 369-377.
  • 7. Imamoglu, E, Simulation design for microalgal protein optimization, Bioengineered, 2015, 6(6), 342-346.
  • 8. Day, J.G, Brand, J.J, Cryopreservation methods for maintaining microalgal cultures. In: Andersen R.A (ed). Algal culturing techniques, Elsevier Academic Press, London, 2005, pp 165-187.
  • 9. Gaget, V, Chiu, Y.T, Lau, M, Humpage, A.R, From an environmental sample to a long-lasting culture: the steps to better isolate and preserve cyanobacterial strains, Journal of Applied Phycology, 2017, 29(1), 309-321.
  • 10. Poncet, J.M, Cryopreservation of the unicellular marine alga, Nannochloropsis oculata, Biotechnology Letters, 2003, 25(23), 2017-2022.
  • 11. Hubalek, Z, Protectants used in the cryopreservation of microorganisms, Cryobiology, 2003, 46(3), 205-229.
  • 12. Salas-Leiva, J.S, Dupré, E, Criopreservación de las microalgas Chaetoceros calcitrans (Paulsen): análisis del efecto de la temperatura de DMSO y régimen de luz durante diferentes períodos de equilibrio, Latin American Journal of Aquatic Research, 2011, 39(2), 271-279.
  • 13. Chen, G, Yue, A, Ruan, Z., Yin, Y, Wang, R, Ren, Y, Zhu, L, Comparison of the effects of different cryoprotectants on stem cells from umbilical cord blood, Stem Cells International, 2016, 2016, 1-7.
  • 14. Imamoglu, E, Demirel, Z., Conk Dalay, M, Process optimization and modeling for the cultivation of Nannochloropsis sp. and Tetraselmis striata via response surface methodology, Journal of Phycology, 2015, 51(3), 442-453.
  • 15. Sarrai, A.E, Hanini, S, Merzouk, N.K, Tassalit, D, Szabó, T, Hernádi, K, Nagy, L, Using central composite experimental design to optimize the degradation of tylosin from aqueous solution by photo-fenton reaction, Materials, 2016; 9(428), 1-11.
  • 16. Malakar, J, Nayak, A.K, Goswami S, Use of response surface methodology in the formulation and optimization of bisoprolol fumarate matrix tablets for sustained drug release, ISRN Pharmaceutics, 2012, 2012, 1-10.
  • 17. Imamoglu, E, Demirel, Z, Dalay M.C, Evaluation of culture conditions of locally isolated Dunaliella salina strain EgeMacc-024, Biochemical Engineering Journal, 2014, 92, 22-27.
  • 18. Saruyama, N, Sakakura, Y, Asano, T, Nishiuchi, T, Sasamoto, H, Kodama, H, Quantification of metabolic activity of cultured plant cells by vital staining with fluorescein diacetate, Analytical Biochemistry, 2013, 441(1), 58-62.
  • 19. Joseph, I, Panigrahi, A, Chandra, P.K, Tolerance of three marine microalgae to cryoprotectant dimethy sulfoxide, methanol and glycerol, Indian Journal of Geo-Marine Sciences, 2000, 29, 243-247.
  • 20. Cañavate, J.P, Lubian, L.M, Tolerance of six marine microalgae to the cryoprotectants dimethyl sulfoxide and methanol, Journal of Phycology, 1994, 30(3), 559-565.

Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis

Year 2018, Volume: 14 Issue: 4, 405 - 412, 28.12.2018
https://doi.org/10.18466/cbayarfbe.426444

Abstract

The preservation of microalgae
in a stable state is a fundamental requirement in pharmaceutical, agricultural,
environmental sciences and different industries. Cryopreservation is widely
stabilized for achieving long-term storage and has been applied to an
increasingly diverse range of microalgae and cell cultures. The continuous storage
of actively growing microalgae strains by routine serial subculture is
relatively time-consuming and this technique has possible contamination risks.
In this study, the optimization of cryopreservation process was carried out for
two different Chlorella strains
using response surface methodology (RSM) with three factors (cryoprotectant
concentration, incubation time and cryopreservation time) including 19 runs.
The
optimal cell viability of C. zofingiensis
was found at the dimethyl sulfoxide (DMSO) concentration of 12.89% at the incubation
time of 8.14 min and with the cryopreservation time of 93.45 day, while C.
saccharophila
was found at the
DMSO concentration of 12.86 % at the incubation time of 7.99 min and at
cryopreservation time of 95.17 day.

References

  • 1. Ravindran, B, Gupta, S.K, Cho, W.M, Kim, J.K, Lee, S.R, Jeong, K.H, Lee, D.J, Choi, H-C, Microalgae Potential and Multiple Roles-Current Progress and Future Prospects-An Overview, Sustainability, 2016, 8(1215), 1-16.
  • 2. Demirel, Z, Demirkaya, C, Imamoglu, E, Dalay, M.C, Diatom cultivation and lipid productivity for non-cryopreserved and cryopreserved cells, Agronomy Research, 2016, 14(4), 1266-1273.
  • 3. Nakanishi, K, Deuchi, K, Kuwano, K, Cryopreservation of four valuable strains of microalgae, including viability and characteristics during 15 years of cryostorage, Journal of Applied Phycology, 2012, 24(6), 1381-1385.
  • 4. Kumari, N, Gupta, M.K, Singh, R.K, Open encapsulation-vitrification for cryopreservation of algae, Cryobiology, 2016, 73(2), 232-239. 5. Kaur, R, Pramanik, K, Sarangi, S.K, Cryopreservation-induced stress on long-term preserved articular cartilage, ISRN Tissue Engineering, 2013, 2013, 1-10.
  • 6. Day, J.G, Fleck, R.A, Benson, E.E, Cryopreservation-recalcitrance in microalgae: novel approaches to identify and avoid cryo-injury, Journal of Applied Phycology, 2000, 12(3-5), 369-377.
  • 7. Imamoglu, E, Simulation design for microalgal protein optimization, Bioengineered, 2015, 6(6), 342-346.
  • 8. Day, J.G, Brand, J.J, Cryopreservation methods for maintaining microalgal cultures. In: Andersen R.A (ed). Algal culturing techniques, Elsevier Academic Press, London, 2005, pp 165-187.
  • 9. Gaget, V, Chiu, Y.T, Lau, M, Humpage, A.R, From an environmental sample to a long-lasting culture: the steps to better isolate and preserve cyanobacterial strains, Journal of Applied Phycology, 2017, 29(1), 309-321.
  • 10. Poncet, J.M, Cryopreservation of the unicellular marine alga, Nannochloropsis oculata, Biotechnology Letters, 2003, 25(23), 2017-2022.
  • 11. Hubalek, Z, Protectants used in the cryopreservation of microorganisms, Cryobiology, 2003, 46(3), 205-229.
  • 12. Salas-Leiva, J.S, Dupré, E, Criopreservación de las microalgas Chaetoceros calcitrans (Paulsen): análisis del efecto de la temperatura de DMSO y régimen de luz durante diferentes períodos de equilibrio, Latin American Journal of Aquatic Research, 2011, 39(2), 271-279.
  • 13. Chen, G, Yue, A, Ruan, Z., Yin, Y, Wang, R, Ren, Y, Zhu, L, Comparison of the effects of different cryoprotectants on stem cells from umbilical cord blood, Stem Cells International, 2016, 2016, 1-7.
  • 14. Imamoglu, E, Demirel, Z., Conk Dalay, M, Process optimization and modeling for the cultivation of Nannochloropsis sp. and Tetraselmis striata via response surface methodology, Journal of Phycology, 2015, 51(3), 442-453.
  • 15. Sarrai, A.E, Hanini, S, Merzouk, N.K, Tassalit, D, Szabó, T, Hernádi, K, Nagy, L, Using central composite experimental design to optimize the degradation of tylosin from aqueous solution by photo-fenton reaction, Materials, 2016; 9(428), 1-11.
  • 16. Malakar, J, Nayak, A.K, Goswami S, Use of response surface methodology in the formulation and optimization of bisoprolol fumarate matrix tablets for sustained drug release, ISRN Pharmaceutics, 2012, 2012, 1-10.
  • 17. Imamoglu, E, Demirel, Z, Dalay M.C, Evaluation of culture conditions of locally isolated Dunaliella salina strain EgeMacc-024, Biochemical Engineering Journal, 2014, 92, 22-27.
  • 18. Saruyama, N, Sakakura, Y, Asano, T, Nishiuchi, T, Sasamoto, H, Kodama, H, Quantification of metabolic activity of cultured plant cells by vital staining with fluorescein diacetate, Analytical Biochemistry, 2013, 441(1), 58-62.
  • 19. Joseph, I, Panigrahi, A, Chandra, P.K, Tolerance of three marine microalgae to cryoprotectant dimethy sulfoxide, methanol and glycerol, Indian Journal of Geo-Marine Sciences, 2000, 29, 243-247.
  • 20. Cañavate, J.P, Lubian, L.M, Tolerance of six marine microalgae to the cryoprotectants dimethyl sulfoxide and methanol, Journal of Phycology, 1994, 30(3), 559-565.
There are 19 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Zeliha Demirel

Esra Imamoglu

İrem Deniz

Meltem Conk Dalay

Publication Date December 28, 2018
Published in Issue Year 2018 Volume: 14 Issue: 4

Cite

APA Demirel, Z., Imamoglu, E., Deniz, İ., Conk Dalay, M. (2018). Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis. Celal Bayar University Journal of Science, 14(4), 405-412. https://doi.org/10.18466/cbayarfbe.426444
AMA Demirel Z, Imamoglu E, Deniz İ, Conk Dalay M. Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis. CBUJOS. December 2018;14(4):405-412. doi:10.18466/cbayarfbe.426444
Chicago Demirel, Zeliha, Esra Imamoglu, İrem Deniz, and Meltem Conk Dalay. “Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella Saccharophila and Chlorella Zofingiensis”. Celal Bayar University Journal of Science 14, no. 4 (December 2018): 405-12. https://doi.org/10.18466/cbayarfbe.426444.
EndNote Demirel Z, Imamoglu E, Deniz İ, Conk Dalay M (December 1, 2018) Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis. Celal Bayar University Journal of Science 14 4 405–412.
IEEE Z. Demirel, E. Imamoglu, İ. Deniz, and M. Conk Dalay, “Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis”, CBUJOS, vol. 14, no. 4, pp. 405–412, 2018, doi: 10.18466/cbayarfbe.426444.
ISNAD Demirel, Zeliha et al. “Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella Saccharophila and Chlorella Zofingiensis”. Celal Bayar University Journal of Science 14/4 (December 2018), 405-412. https://doi.org/10.18466/cbayarfbe.426444.
JAMA Demirel Z, Imamoglu E, Deniz İ, Conk Dalay M. Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis. CBUJOS. 2018;14:405–412.
MLA Demirel, Zeliha et al. “Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella Saccharophila and Chlorella Zofingiensis”. Celal Bayar University Journal of Science, vol. 14, no. 4, 2018, pp. 405-12, doi:10.18466/cbayarfbe.426444.
Vancouver Demirel Z, Imamoglu E, Deniz İ, Conk Dalay M. Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis. CBUJOS. 2018;14(4):405-12.