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Türkiye’nin Konya İli Köpeklerinde Sabin Feldman Boya Testi ile Toxoplasmosis Seroprevalansı

Year 2021, Volume: 2 Issue: 2, 29 - 33, 31.12.2021

Firas Alali Ferda Sevinç Cahit Babür Onur Ceylan

Abstract

Toxoplasma gondii, köpeklerde ve tüm memelilerde toksoplazmozise neden olan hücre içi bir protozoon parazittir. Bu çalışmada Konya ilinde sokak köpeklerinde T. gondii enfeksiyonunun seroprevalansı araştırıldı. Temmuz 2017-Temmuz 2018 döneminde (0-1> yıl ve 1-3> yıl) her iki cinsiyetteki köpeklerden toplam 334 plazma örneği toplandı. Plazma örneklerinde Sabin-Feldman Boya testi ile T. gondii’ye spesifik antikorların varlığı araştırıldı. Toxoplasma gondii’nin seroprevalansı %98,5 bulunurken, bu değerin erkek ve dişi köpeklerde sırasıyla %99,2 ve %98,1 olduğu belirlendi. Pozitif vakalar 0-1 yaş hayvanların 14’ünde (%100), 1-3 yaş grubu hayvanların ise 315’inde (%98,4) tespit edildi ve yaş grupları arasında istatistiksel olarak anlamlı bir fark belirlenmedi (P>0.05). Cinsiyete göre seroprevalans değerleri arasında anlamlı bir fark gözlenmedi (P>0.05). Klinik belirtileri olan hayvanların %98,3'ü (60/61) seropozitif bulundu. Yeni ve eski girişli köpeklerde seropozitif hayvan sayısı 329 (%98,5) [yeni girişli hayvanlarda pozitif sayısı 42/44 (%95,4) ve eski girişli hayvanlarda 287/290 (%99)] olarak tespit edildi. Eski girişli köpeklerde seropozitiflik açısından istatistiksel olarak anlamlı bir fark tespit edilmedi (P>0.05). Çalışmadaki 61 hayvanda arka bacaklarda felç ve atrofi, burun akıntısı, deri lezyonları, kene istilası, kusma, ishal, sinir sistemi bozuklukları ve zayıflama gibi klinik belirtiler tespit edildi.

Keywords

Köpek Toxoplasmosis Klinik bulgular Türkiye

References

  • 1. Ergene O, Celebi B, Kucukaslan I. Seroprevalance of canine brucellosis and toxoplasmosis in female and male dogs and relationship to various factors as parity, abortion and pyometra. Indian Journal of Animal Research. 2019; 53:954-958.‏ doi : 10.18805/ijar.B-707.
  • 2. Dubey JP, Murata FH, Cerqueira-Cézar CK, Kwok OC, Su C. Epidemiologic significance of Toxoplasma gondii infections in turkeys, ducks, ratites and other wild birds: 2009–2020. Parasitology. 2021;148: 1-30. doi:10.1017/S0031182020001961.
  • 3. Aciöz M, Bozkaya F, Babür C. Seroprevalence of Toxoplasma gondii antibodies using Sabin-Feldman dye test among equines in Isparta province, Turkey. Parasitologists United Journal 2021;14:146-150. doi: 10.21608/PUJ.2021.69445.1112 .
  • 4. Halonen SK, Weiss LM. Toxoplasmosis. Garcia HH, Tanowitz HB, Del brutto OH. In: Neuroparasitology and Tropical Neurology. British library. Radarweg, Netherland.Handb Clin Neurol 2013; 114: 125–145.
  • 5. Dubey JP. Toxoplasma gondii infections in chickens (Gallus domesticus): prevalence, clinical disease, diagnosis and public health significance. Zoonoses and Public Health 2010; 57: 60-73. doi:10.1111/j.1863-2378.2009.01274.x.
  • 6. Dubey JP, Beatttie CP. Toxoplasmosis of animals and man. United states: Boca Raton: CRC Press Inc. 1988; p.1-220.
  • 7. Akçay S, Pamukçu M, Baran S. Bir köpekte ilk toxoplasmose observasyonu. Vet Hek Der Derg 1950;20: 245-254.
  • 8. Kolören Z, Dubey JP. A review of toxoplasmosis in humans and animals in Turkey. Parasitology. 2020 Jan;147:12-28. doi:10.1017/S0031182019001318.
  • 9. Pimenta AL, Piza ET, Cardoso RB, Dubey JP. Visceral toxoplasmosis in dogs from Brazil. Veterinary Parasitology1993;45:323-326.doi:10.1016/0304-4017(93)90086-3.
  • 10. Silva NM, Lourenco EV, Silva DA, Mineo JR. Optimisation of cut-off titres in Toxoplasma gondii specific ELISA and IFAT in dog sera using immunoreactivity to SAG-1 antigen as a molecular marker of infection. Veterinary Journal 2002; 163:94-98. doi: 10.1053/tvjl.2001.0629.
  • 11. Dubey JP. Toxoplasmosis in cats and dogs. Proceedings of the World Small Animal Veterinary Association. May, 1-14 ,2005; Mexico City, Mexico.
  • 12. Dubey JP, Lindsay DS, Lappin MR. Toxoplasmosis and other intestinal coccidial infections in cats and dogs. Veterinary Clinics Small Animal Practice 2009; 39: 1009-1034. doi: 10.1016/j.cvsm.2009.08.001.
  • 13. Babur C, Erdal N, Biyikoglu G, Piskin FC. Seroprevalence of toxoplasmosis on stray dogs in İstanbul. Acta Parasitologica Turcica 1997; 21: 413-416.
  • 14. Reiter-Owona I, Petersen E, Joynson D, Aspöck H, Darde ML, et al. The past and present role of the Sabin-Feldman dye test in the serodiagnosis of toxoplasmosis. Bulletin of the World Health Organization 1999; 77: 929-935. 15. Kotresha D, Noordin R. Recombinant proteins in the diagnosis of toxoplasmosis. Apmis 2010;118:529-542. doi:10.1111/j.1600-0463.2010.02629.x.
  • 16. Babür C, Altaş MG, Çelebi B, Sevgili M, Taylan Özkan A, et al. Seroprevalance of Toxoplasmosis, Leishmaniosis and Listeriosis in stray dogs in the province of Sanliurfa, Turkey. Turkish Bulletin of Hygiene and Experimental Biology 2007; 64:11-16.
  • 17. Kilic S, Babür C, Özkan AT, Mamak N. Investigation of anti-Toxoplasma gondii and anti-Leishmania infantum antibodies among Sivas Kangal Dogs. Turkish Journal of Veterinary and Animal Sciences 2008; 32: 299-304.
  • 18. Gicik Y, Sari B, Babür C, Celebı B. The seropositivity of Toxoplasma gondii and Listeria monocytogenes in the dogs of Kars and vicinity. Türkiye Parazitoloji Dergisi 2010 ;34:86-90.
  • 19. Içen H, Babür C, Bademkiran S, Celebi B, Simşek A, et al. Seroprevalance of toxoplasmosis, leishmaiosis and listeriosis in shelter dogs of Diyarbakir, Turkey. Türkiye Parazitoloji Dergisi 2010;34:6-10.
  • 20. Altay K, Babür C, Ataş DA, Beyhan YE, Özkan E. Investigation of seroprevalence of Toxoplasma gondii in dogs in the Province of Sivas. Journal of Etlik Veterinary Microbiology 2013; 24:13-16.
  • 21. Doğan N, Özkan AT, Babür C, Köse C. Seroprevalance of leishmaniosis and toxoplasmosis in healthy appearanced street dogs in Eskisehir. Turkish Bulletin of Hygiene and Experimental Biology 2014; 71: 27-34. Doi:10.5505/TurkHijyen.2014.56833.
  • 22. Langoni H, Matteucci G, Medici B, Camossi LG, Richini-Pereira VB, et al. Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders. Revista da Sociedade Brasileira de Medicina Tropical 2012; 45:365-368. doi:10.1590/S0037-86822012000300016.
  • 23. Oliveira TS, Turchetti AP, Barbosa FB, Bicalho AL, Alencar CA, et al. Cutaneous toxoplasmosis in an immunosuppressed dog. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 2014; 66: 797-800. doi:10.1590/1678-41626891.
  • 24. Huertas-López A, Sukhumavasi W, Álvarez-García G, Martínez-Subiela S, Cano-Terriza D, et al. Seroprevalence of Toxoplasma gondii in outdoor dogs and cats in Bangkok, Thailand. Parasitology 2021;148:843-849.‏ doi:10.1017/S0031182021000421.
  • 25. Aslan G, Altintaş K. Toksoplazmosis teşhisinde Sabin-Feldman testi ve ELISA IgM antikorlarının karşılaştırılması. Genel Tıp Dergisi 2000;10:161-164.
  • 26. Zarra-Nezhad F, Borujeni MP, Mosallanejad B, Hamidinejat H. A seroepidemiological survey of Toxoplasma gondii infection in referred dogs to Veterinary Hospital of Ahvaz, Iran. International Journal of Veterinary Science and Medicine 2017; 5: 148-151. doi:10.1016/j.ijvsm.2017.08.006 .
  • 27. Yan C, Fu LL, Yue CL, Tang RX, Liu YS, et al. Stray dogs as indicators of Toxoplasma gondii distributed in the environment: the first report across an urban-rural gradient in China. Parasites and Vectors 2012; 5: 1-5. doi:10.1186/1756-3305-5-5.

Seroprevalence of Canine Toxoplasmosis by Sabin Feldman Dye Test in Konya Province, Turkey*

Year 2021, Volume: 2 Issue: 2, 29 - 33, 31.12.2021

Firas Alali Ferda Sevinç Cahit Babür Onur Ceylan

Abstract

Toxoplasma gondii (T. gondii) is a protozoan parasite that lives within cells and is the cause of toxoplasmosis in dogs and all animals. The purpose of this research was to determine the seroprevalence of T. gondii infection in stray dogs in the Konya province of Turkey. Between July 2017 and July 2018, 334 plasma samples were taken from dogs of both genders, aged 0-1 and 1-3 years. The samples were tested for T. gondii-specific antibodies using the Sabin-Feldman Dye Test (SFDT). T. gondii seroprevalence was 98.5%, with infection rates of 99.2% in males and 98.1% in females, respectively. Positive cases were 14 (100%) in 0-1 year-old animals and 315 (98.4%) in the 1-3 year age group, with no significant difference between the age groups (P>0.05). Gender differences were not statistically significant (P> 0.05). Seropositivity was detected in 98.3% (60/61) of the animals having clinical symptoms. The number of seropositive cases in new and old entrance dogs was 329 (98.5%), 42/44 (95.4%) in new entry dogs and 287/290 (99%) in old entry dogs. There was no significant difference in the elderly entry dogs (P>0.05). In the current investigation, 61 animals were examined for clinical signs, such as paralysis and atrophy of the hind-limbs, nasal discharge, skin lesions, tick infestations, vomiting, diarrhea, nervous system problems, and emaciation.

Keywords

Dog Toxoplasmosis Clinical signs Turkey

References

  • 1. Ergene O, Celebi B, Kucukaslan I. Seroprevalance of canine brucellosis and toxoplasmosis in female and male dogs and relationship to various factors as parity, abortion and pyometra. Indian Journal of Animal Research. 2019; 53:954-958.‏ doi : 10.18805/ijar.B-707.
  • 2. Dubey JP, Murata FH, Cerqueira-Cézar CK, Kwok OC, Su C. Epidemiologic significance of Toxoplasma gondii infections in turkeys, ducks, ratites and other wild birds: 2009–2020. Parasitology. 2021;148: 1-30. doi:10.1017/S0031182020001961.
  • 3. Aciöz M, Bozkaya F, Babür C. Seroprevalence of Toxoplasma gondii antibodies using Sabin-Feldman dye test among equines in Isparta province, Turkey. Parasitologists United Journal 2021;14:146-150. doi: 10.21608/PUJ.2021.69445.1112 .
  • 4. Halonen SK, Weiss LM. Toxoplasmosis. Garcia HH, Tanowitz HB, Del brutto OH. In: Neuroparasitology and Tropical Neurology. British library. Radarweg, Netherland.Handb Clin Neurol 2013; 114: 125–145.
  • 5. Dubey JP. Toxoplasma gondii infections in chickens (Gallus domesticus): prevalence, clinical disease, diagnosis and public health significance. Zoonoses and Public Health 2010; 57: 60-73. doi:10.1111/j.1863-2378.2009.01274.x.
  • 6. Dubey JP, Beatttie CP. Toxoplasmosis of animals and man. United states: Boca Raton: CRC Press Inc. 1988; p.1-220.
  • 7. Akçay S, Pamukçu M, Baran S. Bir köpekte ilk toxoplasmose observasyonu. Vet Hek Der Derg 1950;20: 245-254.
  • 8. Kolören Z, Dubey JP. A review of toxoplasmosis in humans and animals in Turkey. Parasitology. 2020 Jan;147:12-28. doi:10.1017/S0031182019001318.
  • 9. Pimenta AL, Piza ET, Cardoso RB, Dubey JP. Visceral toxoplasmosis in dogs from Brazil. Veterinary Parasitology1993;45:323-326.doi:10.1016/0304-4017(93)90086-3.
  • 10. Silva NM, Lourenco EV, Silva DA, Mineo JR. Optimisation of cut-off titres in Toxoplasma gondii specific ELISA and IFAT in dog sera using immunoreactivity to SAG-1 antigen as a molecular marker of infection. Veterinary Journal 2002; 163:94-98. doi: 10.1053/tvjl.2001.0629.
  • 11. Dubey JP. Toxoplasmosis in cats and dogs. Proceedings of the World Small Animal Veterinary Association. May, 1-14 ,2005; Mexico City, Mexico.
  • 12. Dubey JP, Lindsay DS, Lappin MR. Toxoplasmosis and other intestinal coccidial infections in cats and dogs. Veterinary Clinics Small Animal Practice 2009; 39: 1009-1034. doi: 10.1016/j.cvsm.2009.08.001.
  • 13. Babur C, Erdal N, Biyikoglu G, Piskin FC. Seroprevalence of toxoplasmosis on stray dogs in İstanbul. Acta Parasitologica Turcica 1997; 21: 413-416.
  • 14. Reiter-Owona I, Petersen E, Joynson D, Aspöck H, Darde ML, et al. The past and present role of the Sabin-Feldman dye test in the serodiagnosis of toxoplasmosis. Bulletin of the World Health Organization 1999; 77: 929-935. 15. Kotresha D, Noordin R. Recombinant proteins in the diagnosis of toxoplasmosis. Apmis 2010;118:529-542. doi:10.1111/j.1600-0463.2010.02629.x.
  • 16. Babür C, Altaş MG, Çelebi B, Sevgili M, Taylan Özkan A, et al. Seroprevalance of Toxoplasmosis, Leishmaniosis and Listeriosis in stray dogs in the province of Sanliurfa, Turkey. Turkish Bulletin of Hygiene and Experimental Biology 2007; 64:11-16.
  • 17. Kilic S, Babür C, Özkan AT, Mamak N. Investigation of anti-Toxoplasma gondii and anti-Leishmania infantum antibodies among Sivas Kangal Dogs. Turkish Journal of Veterinary and Animal Sciences 2008; 32: 299-304.
  • 18. Gicik Y, Sari B, Babür C, Celebı B. The seropositivity of Toxoplasma gondii and Listeria monocytogenes in the dogs of Kars and vicinity. Türkiye Parazitoloji Dergisi 2010 ;34:86-90.
  • 19. Içen H, Babür C, Bademkiran S, Celebi B, Simşek A, et al. Seroprevalance of toxoplasmosis, leishmaiosis and listeriosis in shelter dogs of Diyarbakir, Turkey. Türkiye Parazitoloji Dergisi 2010;34:6-10.
  • 20. Altay K, Babür C, Ataş DA, Beyhan YE, Özkan E. Investigation of seroprevalence of Toxoplasma gondii in dogs in the Province of Sivas. Journal of Etlik Veterinary Microbiology 2013; 24:13-16.
  • 21. Doğan N, Özkan AT, Babür C, Köse C. Seroprevalance of leishmaniosis and toxoplasmosis in healthy appearanced street dogs in Eskisehir. Turkish Bulletin of Hygiene and Experimental Biology 2014; 71: 27-34. Doi:10.5505/TurkHijyen.2014.56833.
  • 22. Langoni H, Matteucci G, Medici B, Camossi LG, Richini-Pereira VB, et al. Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders. Revista da Sociedade Brasileira de Medicina Tropical 2012; 45:365-368. doi:10.1590/S0037-86822012000300016.
  • 23. Oliveira TS, Turchetti AP, Barbosa FB, Bicalho AL, Alencar CA, et al. Cutaneous toxoplasmosis in an immunosuppressed dog. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 2014; 66: 797-800. doi:10.1590/1678-41626891.
  • 24. Huertas-López A, Sukhumavasi W, Álvarez-García G, Martínez-Subiela S, Cano-Terriza D, et al. Seroprevalence of Toxoplasma gondii in outdoor dogs and cats in Bangkok, Thailand. Parasitology 2021;148:843-849.‏ doi:10.1017/S0031182021000421.
  • 25. Aslan G, Altintaş K. Toksoplazmosis teşhisinde Sabin-Feldman testi ve ELISA IgM antikorlarının karşılaştırılması. Genel Tıp Dergisi 2000;10:161-164.
  • 26. Zarra-Nezhad F, Borujeni MP, Mosallanejad B, Hamidinejat H. A seroepidemiological survey of Toxoplasma gondii infection in referred dogs to Veterinary Hospital of Ahvaz, Iran. International Journal of Veterinary Science and Medicine 2017; 5: 148-151. doi:10.1016/j.ijvsm.2017.08.006 .
  • 27. Yan C, Fu LL, Yue CL, Tang RX, Liu YS, et al. Stray dogs as indicators of Toxoplasma gondii distributed in the environment: the first report across an urban-rural gradient in China. Parasites and Vectors 2012; 5: 1-5. doi:10.1186/1756-3305-5-5.
There are 26 citations in total.

Details

Primary Language English
Subjects Veterinary Sciences
Journal Section Research Articles
Authors

Firas Alali 0000-0002-3438-6453

Ferda Sevinç 0000-0001-5426-1685

Cahit Babür 0000-0002-1961-7305

Onur Ceylan 0000-0002-3514-5221

Publication Date December 31, 2021
Submission Date November 20, 2021
Published in Issue Year 2021 Volume: 2 Issue: 2

Cite

Vancouver Alali F, Sevinç F, Babür C, Ceylan O. Seroprevalence of Canine Toxoplasmosis by Sabin Feldman Dye Test in Konya Province, Turkey*. Bozok Vet Sci. 2021;2(2):29-33.
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