Farklı Lokasyonlarda Doğal Olarak Yetişen Sarı Kantaron (Hypericum perforatum L.) Bitki Kısımlarının Toplam Polifenol, Toplam Flavonoid ve Antioksidan Aktiviteleri
Abstract
Tıbbi aromatik bitkilerin büyük bir çoğunluğu antioksidan, antimikrobiyal, antifungal, antidiyabetik, antialerjik, antikanser antienflamatuvar, antimalaryal, antitiroid ve ağrı kesici özelliklere sahip olan sekonder metabolitleri içerdiklerinden sıklıkla farklı üretim alanlarında kullanılmaktadır. Türkiye’de birçok bölgede doğal olarak yetişen ve sarı kantaron olarak da bilinen Hypericum perforatum L. bitkisi de değerli tıbbi aromatik bir bitkidir. Bu çalışmada, farklı eko-coğrafik özelliklere sahip Bayburt ve Kırklareli lokasyonlarından toplanan H. perfo-ratum L. bitkisinin çiçek, yaprak, sap ve kök kısımlarına ait antioksidan aktivitelerin belirlenmesi amaçlanmak-tadır. Farklı bölgelerde doğal olarak yetişen H. perforatum L. kısımlarının karşılaştırıldığı ilk çalışma olma özelliğine sahip bu çalışmada; en yüksek toplam fenolik madde değeri (11.87 mg GAE/g) Kırklareli lokasyo-nundan toplanan H. perforatum L. bitkisinin yaprak örneğinde, en düşük değer ise (4.49 mg GAE/g) Bayburt lokasyonundan toplanan H. perforatum L. sap örneğinde, en yüksek DPPH değeri (1.734 SC50 mg/mL) Bayburt lokasyonundan toplanan H. perforatum L. kök örneğinde, en düşük değer ise (0.259 SC50 mg/mL) Kırklareli lokasyonundan toplanan H. perforatum L. yaprak örneğinde, en yüksek FRAP değeri, (216.97 μmol Fe-SO4.7H2O/g) Kırklareli lokasyonundan toplanan H. perforatum L. yaprak örneğinde, en düşük FRAP değeri ise (60.42 μmol FeSO4.7H2O/g) ise Bayburt lokasyonundan toplanan H. perforatum L. kök örneğinde belirlenmiştir. Çalışmadan elde edilen antioksidan aktivite değerleri incelendiğinde, Kırklareli ilinde doğal olarak yetişen H. perforatum L. bitkisinin, Bayburt ilinde doğal olarak yetişene kıyasla daha tercih edilebilir olduğu sonucu ortaya çıkmaktadır.
References
- Andleeb, Z., Choudhary, S., Naeem, M., Masroor, M., Khan, A., Aftab, T. (2019). A review of medicinal and aromatic plants and their secondary metabolites status under abiotic stress. Journal of Medicinal Plants, 7 (3), 99-106.
- Baytop, T. (1999). Türkiye’de Bitkilerle Tedavi, Nobel Tıp Yayınevi. 2. Baskı. İstanbul, s. 256. Erişim: https://www.ankaranobel.com/images/urunler/turkiyede-bitkiler-ile-tedavi-60a2773112d44.pdf
- Benzie, I. F. F., Strain, J. J. (1996) Ferric reducing ability of plasma (FRAP) as a measure of ‘’antioxidant power’’: The FRAP Assay. Analytical Biochemistry. 239, 70-76.
- Benzie, I., Strain, J. (1999). Ferric reducing/antioxidant power assay: Direct measure of total antioxident activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299, 15-27.
- Burunkaya, B. (2020). Sarı Kantaron (Hypericum Perforatum L.) Fenoliklerinin Karekterizasyonu, Antioksidan Ve Antimikrobiyal Potansiyelinin Belirlenmesi (Yüksek lisans tezi). Adana Alparslan Türkeş Bilim ve Teknoloji Üniversitesi, Fen Bilimleri Enstitüsü, Adana.
- Chimshirova, R., Karsheva, M., Diankov, S., Hinkov, I. (2019). Extractıon Of Valuable Compounds From Bulgarian St. John’s Wort (Hypericum Perforatum L.). Antıoxidant Capacıty And Total Polyphenolıc Content. Journal of Chemical Technology and Metallurgy, 54 (5), 952-961.
- Fukumoto, L.R., Mazza, G. (2000). Assessing antioxidant and prooxidant activities of phenolic compounds. Journal of agricultural and food chemistry 48(8): 3597-3604.
- Grünwald, J. (1999). The World Market for Hypericum Products. Nutraceuticals World, May/June, p. 22-25.
- Gülben, K., Berberoğlu, U., Çelen, O., Mersin H. H. (2008). Incidental papillary microcarcinoma of the thyroid-factors affecting lymph node metastasis. Langenbecks Arch Surg 393, 25-29.
- Kalogeropoulos, N., Yannakopoulou, K., Gioxari, A., Chiou, A., Makris, D. P. (2010). Polyphenol characterization and encapsulation in β-cyclodextrin of aflavonoid-rich Hypericum perforatum (St John’s wort) extract. LWT - Food Science and Technology, 43 (6), 882-889.
- Maslennikov, P. V., Chupakhina, G. N. A., Skrypnik, L. N. (2013). The Content Of Phenolic Compounds İn Medicinal Plants Of A Botanical Garden (Kaliningrad oblast). Biology Bulletin, 41 (2), 133-138.
- Moffat B. (2014). Archaeological Sources for the History of Herbal Medicine Practice: Thecasestudy of StJohn’swort with valerian at Soutra medieval hospital. In Francia S., Stobart A. (eds) Critical Approaches-to the History of Western Herbal Medicine: From Classical Antiquityto the Early Modern Period. Bloomsbury, London, UK, pp. 253-270.
- Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26 (2), 211-219.
- Ng, Q. X., Venkatanarayanan, N., Ho, C. Y. X. (2017). Clinical use of Hypericum perforatum (St John's wort) in depression: A meta-analysis. Journal of affective disorders, 210, 211-221.
- Patocka, J. (2003). The Chemistry, Pharmacology and Toxicology of The Biologically Active Constituents of The Herb Hypericum perforatum L. Journal of Applied Biomedicine, 1:61-70.
- Saddiqe, Z., Naeem, I. ve Maimoona, A. (2010). A review of the antibacterial activity of Hypericum perforatum L. J. Ethnopharmacol. 131 (3), 511-521.
- Sarıkürkçü, C., Locatelli, M., Tartaglia, A., Ferrone, V., Juszczak, A. M., Özer, M. S., Tepe B., Tomczyk, M. (2020). Enzymeand and Biological Activities of the Water Extracts from the Plants Aesculus hippocastanum, Olea europaea and Hypericum perforatum That Are Used as Folk Remedies in Turkey. Molecules, 25 (5), 1202.
- Saygılı, R. (2015). http://cografyaharita.com/ Erişim: 24.02.2022.
- Seyrekoğlu, F., Temiz, H. (2020). Effect of Extraction Conditions on the Phenolic Content and DPPH Radical Scavenging Activity of Hypericum perforatum L. Turkish Journal of Agriculture- Food Scienceand Technology, 8 (1), 226-229.
- Singleton, V. L., Rossi, J. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagent. American Journal of Enology and Viticulture, 16, 144-158.
- Singleton, V. L., Orthofer, R., ve Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152-178.
- Kolayli, S., Can, Z., Yildiz, O., Sahin, H., Karaoglu S.A.(2016) A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys, Journal of Enzyme Inhibition and Medicinal Chemistry, 31:sup3, 96-104.
- Tusevski, O., Todorovska, M., Spasenoski, M., Simic, S. G. (2019). Antioxidant phenolic compounds in Hypericum perforatum L. wild-growing plants collected in the Republic of Macedonia. Biyologica Nyssana, 10 (2), 159-168.
- Yurtvermez, B. (2016). Tarhun (Artemisia dracunculus L.) bitkisinden biyolojik aktivite gösterebilecek sekonder metabolitlerin izolasyonu ve kimyasal yapılarının belirlenmesi (Yüksek lisans Tezi), Ağrı İbrahim Çeçen Üniversitesi Fen Bilimleri Enstitüsü, Ağrı.
- Zdunic, G., Godjevac, D., Savikin, K., Petrovic S. (2017). Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties. Natural Product Communications, 12, 11, 1805-1811.
Total Polyphenol, Total Flavonoid and Antioxidant Activities of St. John's Wort Naturally Grown in Different Locations (Hypericum perforatum L.) Plant Parts
Abstract
The majority of medicinal aromatic plants are frequently used in different production areas as they contain secondary metabolites with antioxidant, antimicrobial, antifungal, antidiabetic, antiallergic, anticancer, anti-inflammatory, antimalarial, antithyroid and pain relief properties. Hypericum perforatum L., also known as St. John's Wort, which grows naturally in many regions in Turkey, is also a valuable medicinal aromatic plant. In this study, it is aimed to determine the antioxidant activities of the flower, leaf, stem and root parts of H. perforatum L. plant collected from Bayburt and Kırklareli locations with different eco-geographical characteristics. In this study, which is the first to compare the parts of H. perforatum L. grown naturally in different regions; The highest total phenolic substance value (11.87 mg GAE/g) was in the leaf sample of H. perforatum L. plant collected from Kırklareli location, and the lowest value (4.49 mg GAE/g) was in H. perforatum L. collected from Bayburt location. In the stem sample, the highest DPPH value (1.734 SC50 mg/mL) was in the H. perforatum L. root sample collected from Bayburt location, and the lowest value (0.259 SC50 mg/mL) in the H. perforatum L. leaf sample collected from the Kırklareli location, the highest FRAP value (216.97 μmol FeSO4.7H2O/g) in H. perforatum L. leaf sample collected from Kırklareli location, and the lowest FRAP value (60.42 µmol FeSO4.7H2O/g) in H. perforatum L. collected from Bayburt location. determined in the root sample. When the antioxidant activity values obtained from the study are examined, it is concluded that the naturally grown H. perforatum L. plant in Kırklareli is more preferable than the naturally grown plant in Bayburt.
References
- Andleeb, Z., Choudhary, S., Naeem, M., Masroor, M., Khan, A., Aftab, T. (2019). A review of medicinal and aromatic plants and their secondary metabolites status under abiotic stress. Journal of Medicinal Plants, 7 (3), 99-106.
- Baytop, T. (1999). Türkiye’de Bitkilerle Tedavi, Nobel Tıp Yayınevi. 2. Baskı. İstanbul, s. 256. Erişim: https://www.ankaranobel.com/images/urunler/turkiyede-bitkiler-ile-tedavi-60a2773112d44.pdf
- Benzie, I. F. F., Strain, J. J. (1996) Ferric reducing ability of plasma (FRAP) as a measure of ‘’antioxidant power’’: The FRAP Assay. Analytical Biochemistry. 239, 70-76.
- Benzie, I., Strain, J. (1999). Ferric reducing/antioxidant power assay: Direct measure of total antioxident activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299, 15-27.
- Burunkaya, B. (2020). Sarı Kantaron (Hypericum Perforatum L.) Fenoliklerinin Karekterizasyonu, Antioksidan Ve Antimikrobiyal Potansiyelinin Belirlenmesi (Yüksek lisans tezi). Adana Alparslan Türkeş Bilim ve Teknoloji Üniversitesi, Fen Bilimleri Enstitüsü, Adana.
- Chimshirova, R., Karsheva, M., Diankov, S., Hinkov, I. (2019). Extractıon Of Valuable Compounds From Bulgarian St. John’s Wort (Hypericum Perforatum L.). Antıoxidant Capacıty And Total Polyphenolıc Content. Journal of Chemical Technology and Metallurgy, 54 (5), 952-961.
- Fukumoto, L.R., Mazza, G. (2000). Assessing antioxidant and prooxidant activities of phenolic compounds. Journal of agricultural and food chemistry 48(8): 3597-3604.
- Grünwald, J. (1999). The World Market for Hypericum Products. Nutraceuticals World, May/June, p. 22-25.
- Gülben, K., Berberoğlu, U., Çelen, O., Mersin H. H. (2008). Incidental papillary microcarcinoma of the thyroid-factors affecting lymph node metastasis. Langenbecks Arch Surg 393, 25-29.
- Kalogeropoulos, N., Yannakopoulou, K., Gioxari, A., Chiou, A., Makris, D. P. (2010). Polyphenol characterization and encapsulation in β-cyclodextrin of aflavonoid-rich Hypericum perforatum (St John’s wort) extract. LWT - Food Science and Technology, 43 (6), 882-889.
- Maslennikov, P. V., Chupakhina, G. N. A., Skrypnik, L. N. (2013). The Content Of Phenolic Compounds İn Medicinal Plants Of A Botanical Garden (Kaliningrad oblast). Biology Bulletin, 41 (2), 133-138.
- Moffat B. (2014). Archaeological Sources for the History of Herbal Medicine Practice: Thecasestudy of StJohn’swort with valerian at Soutra medieval hospital. In Francia S., Stobart A. (eds) Critical Approaches-to the History of Western Herbal Medicine: From Classical Antiquityto the Early Modern Period. Bloomsbury, London, UK, pp. 253-270.
- Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26 (2), 211-219.
- Ng, Q. X., Venkatanarayanan, N., Ho, C. Y. X. (2017). Clinical use of Hypericum perforatum (St John's wort) in depression: A meta-analysis. Journal of affective disorders, 210, 211-221.
- Patocka, J. (2003). The Chemistry, Pharmacology and Toxicology of The Biologically Active Constituents of The Herb Hypericum perforatum L. Journal of Applied Biomedicine, 1:61-70.
- Saddiqe, Z., Naeem, I. ve Maimoona, A. (2010). A review of the antibacterial activity of Hypericum perforatum L. J. Ethnopharmacol. 131 (3), 511-521.
- Sarıkürkçü, C., Locatelli, M., Tartaglia, A., Ferrone, V., Juszczak, A. M., Özer, M. S., Tepe B., Tomczyk, M. (2020). Enzymeand and Biological Activities of the Water Extracts from the Plants Aesculus hippocastanum, Olea europaea and Hypericum perforatum That Are Used as Folk Remedies in Turkey. Molecules, 25 (5), 1202.
- Saygılı, R. (2015). http://cografyaharita.com/ Erişim: 24.02.2022.
- Seyrekoğlu, F., Temiz, H. (2020). Effect of Extraction Conditions on the Phenolic Content and DPPH Radical Scavenging Activity of Hypericum perforatum L. Turkish Journal of Agriculture- Food Scienceand Technology, 8 (1), 226-229.
- Singleton, V. L., Rossi, J. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagent. American Journal of Enology and Viticulture, 16, 144-158.
- Singleton, V. L., Orthofer, R., ve Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152-178.
- Kolayli, S., Can, Z., Yildiz, O., Sahin, H., Karaoglu S.A.(2016) A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys, Journal of Enzyme Inhibition and Medicinal Chemistry, 31:sup3, 96-104.
- Tusevski, O., Todorovska, M., Spasenoski, M., Simic, S. G. (2019). Antioxidant phenolic compounds in Hypericum perforatum L. wild-growing plants collected in the Republic of Macedonia. Biyologica Nyssana, 10 (2), 159-168.
- Yurtvermez, B. (2016). Tarhun (Artemisia dracunculus L.) bitkisinden biyolojik aktivite gösterebilecek sekonder metabolitlerin izolasyonu ve kimyasal yapılarının belirlenmesi (Yüksek lisans Tezi), Ağrı İbrahim Çeçen Üniversitesi Fen Bilimleri Enstitüsü, Ağrı.
- Zdunic, G., Godjevac, D., Savikin, K., Petrovic S. (2017). Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties. Natural Product Communications, 12, 11, 1805-1811.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | Research |
| Authors | |
| Publication Date | December 28, 2022 |
| Published in Issue | Year 2022 Volume: 11 Issue: 2 |
