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The determination of Parainfluenza-3 Viral Antigens Using Immunohistochemical Methods in Cattle Lungs in Erzurum Region

Year 2018, Volume: 13 Issue: 3, 309 - 316, 25.12.2018
https://doi.org/10.17094/ataunivbd.364602

Abstract

The aim of this study was to investigate immunohistochemically the presence and the prevalence of Parainfluenza-

3 (PI-3) in the lungs of cattle breeding in the Erzurum region. For this aim, the lung samples of 600 cattle slaughtered in

abattoirs in Erzurum province were examined by collecting and pneumonia was macroscopically observed in 75 (12.5%) lung

samples. 58 interstitial pneumonia, 10 broncho-interstitial pneumonia, 2 catarrhal pneumonia, 2 fibrinous pneumonia, and 3

granulomatous pneumonia were observed in the histopathological evaluation of these lungs examined macroscopically.

Immunohistochemical staining was performed to pneumonia detected lung sections in terms of PI-3 virus and

immunopositivity was detected in 7 (9.33%) lung samples. Immunopositivity was observed in 6 (10.34%) interstitial

pneumonia and 1 (10%) broncho-interstitial pneumonia in lung samples. Immunopositivity in the lungs which were positive

for PI-3 viral antigens was found in the cytoplasm of bronchi and bronchioles epithelium, in inflammation cells in bronchusassociated

lymphoid tissue (BALT) around bronchioles, in the alveoli, in the macrophages in the interstitial area. Furthermore,

these positivities were observed in the inflammatory exudate in the bronchial lumen in the broncho-interstitial pneumonia

lung samples. In conclusion, it was determined that the presence of PI-3 viral antigens has an important place in agents which

occur pneumonia in cattle in Erzurum region and it is thought that this agent should not be ignored in the protection and

treatment methods against the bovine respiratory disease complex.

References

  • 1. Brodersen BW, Kelling CL. Effect of concurrent experimentally induced bovine respiratory syncytial virus and bovine viral diarrhea virus infection on respiratory tract and enteric diseases in calves. American journal of veterinary research. 1998;59(11):1423-30.
  • 2. Carriere PD, Maxie M, Wilkie B, Savan M, Valli V, Johnson J. Exposure of calves to aerosols of parainfluenza-3 virus and Pasteurella haemolytica. Canadian Journal of Comparative Medicine. 1983;47(4):422.
  • 3. Zhu Y-M, Yu Z, Cai H, Gao Y-R, Dong X-M, Li Z-L, et al. Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China. Virology journal. 2011;8(1):557.
  • 4. Maxie M., Jubb K. Jubb, Kennedy and Palmer’s Pathology of Domestic Animals. 5th ed. ed. Philadelphia2007. 523-629 p.
  • 5. Zachary JF, McGavin MD. Pathologic Basis of Veterinary Disease: Elsevier Health Sciences; 2006. 462-532 p.
  • 6. Fulton RW. Bovine respiratory disease research (1983–2009). Animal Health Research Reviews. 2009;10(2):131-9.
  • 7. Mosier D. Review of BRD pathogenesis: the old and the new. Animal health research reviews. 2015;15(2):166-8.
  • 8. Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA. Virus taxonomy: VIIIth report of the International Committee on Taxonomy of Viruses: Academic Press; 2005.
  • 9. Fulton RW, Purdy C, Confer AW, Saliki J, Loan RW, Briggs RE, et al. Bovine viral diarrhea viral infections in feeder calves with respiratory disease: interactions with Pasteurella spp., parainfluenza-3 virus, and bovine respiratory syncytial virus. Canadian Journal of Veterinary Research. 2000;64(3):151.
  • 10. Mahony TJ, McCarthy FM, Gravel JL, West L, Young PL. Construction and manipulation of an infectious clone of the bovine herpesvirus 1 genome maintained as a bacterial artificial chromosome. Journal of virology. 2002;76(13):6660-8.
  • 11. Snowder G, Van Vleck LD, Cundiff L, Bennett G, Koohmaraie M, Dikeman M. Bovine respiratory disease in feedlot cattle: phenotypic, environmental, and genetic correlations with growth, carcass, and longissimus muscle palatability traits. Journal of animal science. 2007;85(8):1885-92.
  • 12. Maidana SS, Lomonaco PM, Combessies G, Craig MI, Diodati J, Rodriguez D, et al. Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina. BMC veterinary research. 2012;8(1):83.
  • 13. Oem J-K, Lee E-Y, Lee K-K, Kim S-H, Lee M-H, Hyun B-H. Molecular characterization of a Korean bovine parainfluenza virus type 3 isolate. Veterinary microbiology. 2013;162(1):224-7.
  • 14. Murphy FA, Gibbs EPJ, Horzinek MC, Studdert MJ. Veterinary virology: Academic press; 1999.
  • 15. Gülbahar Y, Çabalar M, Ertürk A. Detection by immunoperoxidase technique of parainfluenza type-3 virus and respiratory syncytial virus antigens in naturally occurring pneumonia in lambs. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi. 2002;13:74-7.
  • 16. Presnell JK, Schreibman, M.P. Humason's Animal Tissue Techniques. 5th ed. Baltimore and London: Johns Hopkins University Press; 1997.
  • 17. Zachary JF, Donald M, Gavin M. Pathologic basis of veterinary disease. 4th ed. ed: Elsevier Health Sciences; 2006. 462-558 p.
  • 18. Martin W. Respiratory infections of sheep. Comparative immunology, microbiology and infectious diseases. 1996;19(3):171-9.
  • 19. Bryson D, McNulty M, Ball H, Neill S, Connor T, Cush P. The experimental production of pneumonia in calves by intranasal inoculation of parainfluenza type III virus. The Veterinary Record. 1979;105(25-26):566-73.
  • 20. Ghram A, Reddy P, Morrill J, Blecha F, Minocha H. Bovine herpesvirus-1 and parainfluenza-3 virus interactions: clinical and immunological response in calves. Canadian Journal of Veterinary Research. 1989;53(1):62.
  • 21. Çeribasi S, Özkaraca M, Özer H, Çeribasi AO. Prevalence of parainfluenza type 3 virus antigens in pneumonic caprine lungs by direct immunofluorescence and immunoperoxidase techniques. Small ruminant research. 2012;108(1):127-32.
  • 22. Aly N, Shehab G, El-Rahim I. Bovine viral diarrhoea, bovine herpesvirus and parainfluenza-3 virus infection in three cattle herds in Egypt in 2000. Revue scientifique et technique-Office international des épizooties. 2003;22(3):879-92.
  • 23. Ceribasi A, Ozkaraca M, Ceribasi S, Ozer H. Histopathologic, immunoperoxidase and immunofluorescent examinations on natural cattle pneumonia originated from Parainfluenza type 3, Respiratory Syncytial virus, Adenovirus type 3 and Herpesvirus type 1. Revue de Médecine Vétérinaire. 2014;165:201-12.
  • 24. Yildirim Y, Yilmaz V, Majarashin ARF. Kuzeydoğu Anadolu Bölgesi Sınır İllerinde Bulunan Sığırlarda Viral Solunum Sistemi Enfeksiyonlarının Seroprevalansı. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2009;15(4):601-6.
  • 25. Çabalar M, Can-Şahna K. Doğu ve Güneydoğu Anadolu Bölgesinde süt sığırlarında Parainfluenza Virus -3, Bovine Herpes Virus -1 ve Respiratory Syncytial Virüs Enfeksiyonlarının Seroepidemiyolojisi. . Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi. 2000;11:101-5.
  • 26. Haines DM, Chelack BJ. Technical considerations for developing enzyme immunohistochemical staining procedures on formalin-fixed paraffin-embedded tissues for diagnostic pathology. Journal of Veterinary Diagnostic Investigation. 1991;3(1):101-12.
  • 27. Haines DM, Kendall JC, Remenda BW, Breker-Klassen MM, Clark EG. Monoclonal and polyclonal antibodies for immunohistochemical detection of bovine parainfluenza type 3 virus in frozen and formalin-fixed paraffin-embedded tissues. Journal of Veterinary Diagnostic Investigation. 1992;4(4):393-9.

Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi

Year 2018, Volume: 13 Issue: 3, 309 - 316, 25.12.2018
https://doi.org/10.17094/ataunivbd.364602

Abstract

Bu çalışmada, Erzurum yöresinde yetiştirilen sığırların akciğerlerinde Parainfluenza-3 (PI-3) etkeninin immunohistokimyasal olarak varlığının ve yaygınlığının araştırılması amaçlanmıştır. Bu amaçla, Erzurum ilinde faaliyet gösteren kesimhanelerde kesimi yapılan 600 sığıra ait akciğer örneği toplanarak incelendi ve makroskobik olarak 75 (%12.5) akciğer örneğinde pnömoni bulgusuna rastlandı. Makroskobik olarak incelenen bu akciğerlerin histopatolojik incelemesinde 58 intersitisyel pnömoni, 10 bronkointersitisyel pnömoni, 2 kataral bronkopnömoni, 2 fibrinli pnömoni ve 3 granulomatöz pnömoni olgusu gözlendi. Pnömoni saptanan akciğer kesitlerine PI- 3 virusu yönünden immunohistokimyasal boyama yapıldı ve 7 (%9.33) akciğer örneğinde immunpozitiflikler belirlendi. İmmünpozitifliklere intersitisyel pnömoni gözlenen 6 (%10.34), bronkointersitisyel pnömoni gözlenen 1 (%10) akciğer örneğinde rastlandı. PI-3 viral antijeni pozitif olan akciğerlerde immunpozitifliklere bronş ve bronşiyol epitellerinin sitoplazmasında, bronşiyol çevresindeki bronşiyoalveolar lenfoid dokudaki (BALT) yangı hücrelerinde, alveollerde, intersitisyel alandaki makrofajlarda rastlandı. Ayrıca bu pozitiflikler bronkointersitisyel pnömoni gözlenen akciğer örneklerinde bronşiyol lümenindeki yangısal eksudatta gözlendi. Sonuç olarak; Erzurum yöresindeki sığırlarda pnömoni oluşturan etkenler içerisinde PI-3 viral antijen varlığının önemli bir yere sahip olduğu belirlenmiş ve sığırlardaki solunum sistemi hastalık kompleksine karşı yapılan koruma ve tedavi yöntemlerinde bu etkenin göz ardı edilmemesi gerektiği düşünülmüştür.

References

  • 1. Brodersen BW, Kelling CL. Effect of concurrent experimentally induced bovine respiratory syncytial virus and bovine viral diarrhea virus infection on respiratory tract and enteric diseases in calves. American journal of veterinary research. 1998;59(11):1423-30.
  • 2. Carriere PD, Maxie M, Wilkie B, Savan M, Valli V, Johnson J. Exposure of calves to aerosols of parainfluenza-3 virus and Pasteurella haemolytica. Canadian Journal of Comparative Medicine. 1983;47(4):422.
  • 3. Zhu Y-M, Yu Z, Cai H, Gao Y-R, Dong X-M, Li Z-L, et al. Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China. Virology journal. 2011;8(1):557.
  • 4. Maxie M., Jubb K. Jubb, Kennedy and Palmer’s Pathology of Domestic Animals. 5th ed. ed. Philadelphia2007. 523-629 p.
  • 5. Zachary JF, McGavin MD. Pathologic Basis of Veterinary Disease: Elsevier Health Sciences; 2006. 462-532 p.
  • 6. Fulton RW. Bovine respiratory disease research (1983–2009). Animal Health Research Reviews. 2009;10(2):131-9.
  • 7. Mosier D. Review of BRD pathogenesis: the old and the new. Animal health research reviews. 2015;15(2):166-8.
  • 8. Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA. Virus taxonomy: VIIIth report of the International Committee on Taxonomy of Viruses: Academic Press; 2005.
  • 9. Fulton RW, Purdy C, Confer AW, Saliki J, Loan RW, Briggs RE, et al. Bovine viral diarrhea viral infections in feeder calves with respiratory disease: interactions with Pasteurella spp., parainfluenza-3 virus, and bovine respiratory syncytial virus. Canadian Journal of Veterinary Research. 2000;64(3):151.
  • 10. Mahony TJ, McCarthy FM, Gravel JL, West L, Young PL. Construction and manipulation of an infectious clone of the bovine herpesvirus 1 genome maintained as a bacterial artificial chromosome. Journal of virology. 2002;76(13):6660-8.
  • 11. Snowder G, Van Vleck LD, Cundiff L, Bennett G, Koohmaraie M, Dikeman M. Bovine respiratory disease in feedlot cattle: phenotypic, environmental, and genetic correlations with growth, carcass, and longissimus muscle palatability traits. Journal of animal science. 2007;85(8):1885-92.
  • 12. Maidana SS, Lomonaco PM, Combessies G, Craig MI, Diodati J, Rodriguez D, et al. Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina. BMC veterinary research. 2012;8(1):83.
  • 13. Oem J-K, Lee E-Y, Lee K-K, Kim S-H, Lee M-H, Hyun B-H. Molecular characterization of a Korean bovine parainfluenza virus type 3 isolate. Veterinary microbiology. 2013;162(1):224-7.
  • 14. Murphy FA, Gibbs EPJ, Horzinek MC, Studdert MJ. Veterinary virology: Academic press; 1999.
  • 15. Gülbahar Y, Çabalar M, Ertürk A. Detection by immunoperoxidase technique of parainfluenza type-3 virus and respiratory syncytial virus antigens in naturally occurring pneumonia in lambs. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi. 2002;13:74-7.
  • 16. Presnell JK, Schreibman, M.P. Humason's Animal Tissue Techniques. 5th ed. Baltimore and London: Johns Hopkins University Press; 1997.
  • 17. Zachary JF, Donald M, Gavin M. Pathologic basis of veterinary disease. 4th ed. ed: Elsevier Health Sciences; 2006. 462-558 p.
  • 18. Martin W. Respiratory infections of sheep. Comparative immunology, microbiology and infectious diseases. 1996;19(3):171-9.
  • 19. Bryson D, McNulty M, Ball H, Neill S, Connor T, Cush P. The experimental production of pneumonia in calves by intranasal inoculation of parainfluenza type III virus. The Veterinary Record. 1979;105(25-26):566-73.
  • 20. Ghram A, Reddy P, Morrill J, Blecha F, Minocha H. Bovine herpesvirus-1 and parainfluenza-3 virus interactions: clinical and immunological response in calves. Canadian Journal of Veterinary Research. 1989;53(1):62.
  • 21. Çeribasi S, Özkaraca M, Özer H, Çeribasi AO. Prevalence of parainfluenza type 3 virus antigens in pneumonic caprine lungs by direct immunofluorescence and immunoperoxidase techniques. Small ruminant research. 2012;108(1):127-32.
  • 22. Aly N, Shehab G, El-Rahim I. Bovine viral diarrhoea, bovine herpesvirus and parainfluenza-3 virus infection in three cattle herds in Egypt in 2000. Revue scientifique et technique-Office international des épizooties. 2003;22(3):879-92.
  • 23. Ceribasi A, Ozkaraca M, Ceribasi S, Ozer H. Histopathologic, immunoperoxidase and immunofluorescent examinations on natural cattle pneumonia originated from Parainfluenza type 3, Respiratory Syncytial virus, Adenovirus type 3 and Herpesvirus type 1. Revue de Médecine Vétérinaire. 2014;165:201-12.
  • 24. Yildirim Y, Yilmaz V, Majarashin ARF. Kuzeydoğu Anadolu Bölgesi Sınır İllerinde Bulunan Sığırlarda Viral Solunum Sistemi Enfeksiyonlarının Seroprevalansı. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2009;15(4):601-6.
  • 25. Çabalar M, Can-Şahna K. Doğu ve Güneydoğu Anadolu Bölgesinde süt sığırlarında Parainfluenza Virus -3, Bovine Herpes Virus -1 ve Respiratory Syncytial Virüs Enfeksiyonlarının Seroepidemiyolojisi. . Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi. 2000;11:101-5.
  • 26. Haines DM, Chelack BJ. Technical considerations for developing enzyme immunohistochemical staining procedures on formalin-fixed paraffin-embedded tissues for diagnostic pathology. Journal of Veterinary Diagnostic Investigation. 1991;3(1):101-12.
  • 27. Haines DM, Kendall JC, Remenda BW, Breker-Klassen MM, Clark EG. Monoclonal and polyclonal antibodies for immunohistochemical detection of bovine parainfluenza type 3 virus in frozen and formalin-fixed paraffin-embedded tissues. Journal of Veterinary Diagnostic Investigation. 1992;4(4):393-9.
There are 27 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Araştırma Makaleleri
Authors

Selim Çomaklı

Yavuz Selim Sağlam

Mustafa Özkaraca

Serdar Altun

Publication Date December 25, 2018
Published in Issue Year 2018 Volume: 13 Issue: 3

Cite

APA Çomaklı, S., Sağlam, Y. S., Özkaraca, M., Altun, S. (2018). Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 13(3), 309-316. https://doi.org/10.17094/ataunivbd.364602
AMA Çomaklı S, Sağlam YS, Özkaraca M, Altun S. Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. December 2018;13(3):309-316. doi:10.17094/ataunivbd.364602
Chicago Çomaklı, Selim, Yavuz Selim Sağlam, Mustafa Özkaraca, and Serdar Altun. “Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13, no. 3 (December 2018): 309-16. https://doi.org/10.17094/ataunivbd.364602.
EndNote Çomaklı S, Sağlam YS, Özkaraca M, Altun S (December 1, 2018) Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13 3 309–316.
IEEE S. Çomaklı, Y. S. Sağlam, M. Özkaraca, and S. Altun, “Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi”, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol. 13, no. 3, pp. 309–316, 2018, doi: 10.17094/ataunivbd.364602.
ISNAD Çomaklı, Selim et al. “Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 13/3 (December 2018), 309-316. https://doi.org/10.17094/ataunivbd.364602.
JAMA Çomaklı S, Sağlam YS, Özkaraca M, Altun S. Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2018;13:309–316.
MLA Çomaklı, Selim et al. “Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol. 13, no. 3, 2018, pp. 309-16, doi:10.17094/ataunivbd.364602.
Vancouver Çomaklı S, Sağlam YS, Özkaraca M, Altun S. Erzurum Yöresinde Sığır Akciğerlerinde Parainfluenza-3 Viral Antijenlerinin İmmunohistokimyasal Yöntemlerle Belirlenmesi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2018;13(3):309-16.