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Beta-Sitronellol ve (-)-Menton Monoterpenlerinin İnsan Meme Kanseri (MCF-7) Hücre Hattında Sitotoksik Etkilerinin İncelenmesi

Year 2019, Volume: 3 Issue: 2, 111 - 119, 27.05.2019

Abstract

Amaç: Monoterpenler, ilaç, gıda veya kozmetik endüstrisinde kullanılan,
bitki esans yağlarının ana bileşenleridir.
Birçok terpenoid,
antialerjik, antiviral, anti-inflamatuar ve antikanser gibi çeşitli
farmakolojik aktiviteler sergiler. Bununla birlikte, bu bileşiklerin
insan meme kanseri (MCF-7)
hücreleri üzerine sitotoksik etkileri ile ilgili yeterli çalışma
bulunmamaktadır. Bu çalışmanın amacı,
Beta-Sitronellol
(BS) and (-)-Menton (MNT) monoterpenlerinin potansiyel sitotoksik etkilerini
MCF-7 hücre hattında
3-(4,5-dimethylthiazolyl-2)-2,5
diphenyl tetrazolyum bromid (MTT) testi ile incelemektir.

Yöntem: Bu çalışmada BS ve MNT monoterpenlerinin sitotoksik etkileri, MCF-7
hücre hattında 24 ve 48 saat muamele sürelerinde MTT testi ile analiz edilmiştir. Bunun için hücrelere BS (
2, 10, 50, 250, 1250 μg/mL) ve MNT (16, 80, 400, 2000, 10.000 μg/mL)’nin farklı
konsantrasyonları
uygulanmıştır.

Bulgular:
BS ve MNT, 24 saatlik uygulama süresinde tüm konsantrasyonlarda, (%) hücre
canlılığını önemli düzeyde azaltmıştır. Ayrıca 48 saatlik uygulamada,
BS (250, 1250 μg/mL) ve MNT (16, 400, 2000, 10.000 μg/mL), (%) hücre canlılığını
anlamlı oranda düşürmüştür.







Sonuç: Sonuç olarak; BS ve MNT, her iki muamele süresinde de MCF-7
hücreleri üzerinde sitotoksik etki göstermiştir. Ancak sitotoksisitenin
belirlenmesine yönelik farklı hücre hatlarında daha fazla çalışma yapılmalıdır.

References

  • Gherlardini, C., Galeotti, N., Mazzanti, G. (2001). Local anaesthetic activity of monoterpenes and phenylpropanes of essential oils. Planta Medica, 67, 564–566.
  • Queiroz, T.B., Santos, G.F., Ventura, S.C., Hiruma-Lima, C.A., Gaivão, I.O.M., Maistro, E.L. (2017). Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2). Genetics and Molecular Research, 16 (3), gmr16039777, DOI http://dx.doi.org/10.4238/gmr16039777.
  • Bakkali, F., Averbeck, S., Averbeck, D., Idaomar, M. (2008). Biological effects of essential oils-a review. Food and Chemical Toxicology, 46, 446-475 https://doi.org/10.1016/j.fct.2007.09.106.
  • Slamenova, D., Horvathova, E., Wsolova, L., Sramkova, M., Navarova, J. (2009). Investigation of anti-oxidative, cytotoxic, DNA-damaging and DNA-protective effects of plant volatiles eugenol and borneol in human-derived HepG2, Caco-2 and VH10 cell lines. Mutation Research, 677, 46–52.
  • Unlu, M., Ergene, E., Unlu, G.V., Zeytinoglu, H.S., Vural, N. (2010). Composition, antimicrobial activity and in vitro cytotoxicity of essential oil from Cinnamomum zeylanicum Blume (Lauraceae). Food and Chemical Toxicology, 48, 3274–3280.
  • Balasundram, N., Sundram, K., Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry, 99(1), 191-203.
  • Alu’datt, M.H., Rababah, T., Alhamad, M.N., Al-Mahasneh, M.A., Almajwal, A., Gammoh, S., Ereifej, K., Johargy, A., Alli, I. (2017). A review of phenolic compounds in oil-bearing plants: Distribution, identification and occurrence of phenolic compounds. Food Chemistry, 218, 99–106.
  • Crowell, P.L. (1999). Prevention and therapy of cancer by diethary monoterpenes. The Journal of Nutrition, 129, 775S–778S.
  • Lampe, J.W. (2003). Spicing up a vegetarian diet: chemopreventive effects of phytochemicals. American Society for Clinical Nutrition, 78, 579S–583S.
  • Nikolic, B., Mitic-Culafic, D., Vukovic-Gacic, B., Knezevic-Vukcevic, J. (2011). Modulation of genotoxicity and DNA repair by plant monoterpenes camphor, eucalyptol and thujone in Escherichia coli and mammalian cells. Food and Chemical Toxicology, 49, 2035-2045.
  • Ribeiro-Filho, H.V., Silva de, C.M.S., Siqueira de, R.B., Lahlou, S., Santos, A.A., Magalhães, P.J.C. (2016). Biphasic cardiovascularandrespiratoryeffectsinducedby β-citronellol. European Journal of Pharmacology. 775, 96–105.
  • Elsharif, S.A., Buettner, A. (2017). Influence of the chemical structure on the odor characters of β-citronellol and its oxygenated derivatives. Food Chemistry, 232, 704–711.
  • Murakami, A., Furukawa, J., Kawasaki, Y., Ota, R. (2013). Flavoring agent with natural fruit-like hop aroma and manufacturing method of beverages. In: Kirin Brewery Co., Ltd. Japan. pp. 19.
  • Brito, R.G., Guimarães, A.G., Quintans, J.S.S., Santos, M.R.V., Sousa, D.P., Badaue-Passos, D., Quintans, L.J. (2012). Citronellol, a monoterpene alcohol, reduces nociceptive and inflammatory activities in rodents. Journal of Natural Medicines, 66 (4), 637–644.
  • Kamatou, G.P.P., Vermaak, I., Viljoen, A.M., Lawrence, B.M. (2013). Menthol: A simple monoterpene with remarkable biological properties. Phytochemistry, 96, 15–25.
  • Croteau, R.B., Davis, E.M., Ringer, K.L., Wildung, M.R. (2005). (-)-Menthol biosynthesis and molecular genetics. Naturwissenschaften, 92, 562–577.
  • Lawrence, B.M. (2013). The story of India’s mint oils and menthol. Perfumer and Flavorist, 38 (1), 26–35.
  • Thoppil, R.J., Bishayee, A. (2011). Terpenoids as potential chemopreventive and therapeutic agents in liver cancer. World journal of hepatology, 3, 228-249.
  • Murthy, K.N.C., Jayaprakasha, G.K., Patil, B.S. (2012). D-limonene rich volatile oil from blood oranges inhibits angiogenesis, metastasis and cell death in human colon cancer cells. Life Sciences, 91, 429- 439.
  • Steward, W.P., Brown, K. (2013). Cancer chemoprevention: a rapidly evolving field. British Journal of Cancer, 109, 1-7.
  • Maßberg, D., Simon, A., Häussinger, D., Keitel, V., Gisselmann, G., Conrad, H., Hatt, H. (2015). Monoterpene (-)-citronellal affects hepatocarcinoma cell signaling via an olfactory receptor. Archives of Biochemistry and Biophysics, 566, 100–109.
  • Meng, C., He, Y., Wei, Z., Lu, Y., Du, F., Ou, G., Wang, N., Luo, X.G., Ma, W. (2018). Zhang TC. He H. MRTF-A mediates the activation of COL1A1 expression stimulated by multiple signaling pathways in human breast cancer cells. Biomedicine & Pharmacotherapy, 104, 718-728.
  • Song, Y., Lu, M., Qiu, H., Yin, J., Luo, K., Zhang, Z., Jia, X., Zheng, G., Liu, H., He, Z. (2018). Activation of FOXO3a reverses 5-Fluorouracil resistance in human breast cancer cells. Experimental and Molecular Pathology, 105, 57–62.
  • https://www.sigmaaldrich.com/catalog/product/aldrich/c83201?lang=en&region=TR
  • https://www.sigmaaldrich.com/catalog/product/aldrich/218235?lang=en&region=TR
  • Mossman, T. (1983). Rapid colometric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65, 55-63.
  • Mamur, S., Yuzbasioglu, D., Yılmaz, S., Erikel, E., Unal, F. (2018). Assessment of Cytotoxic and Genotoxic Effects of Enniatin-A In Vitro. Food Additives & Contaminants: Part A, 35 (8), 1633-1644, https://doi.org/10.1080/19440049.2018.1486513.
  • Liu, S.J., Liao, Z.X., Liu, C., Sun, J.Y. (2015). Chemical constituents, anticancer, antimicrobial and antioxidant activities of essential oil from Anaphalis lacteal grown in Qinghai-Tibet Plateau. Natural Product Research, 29, 1369-1371.
  • Rabi, T., Bishayee, A. (2009). Terpenoids and breast cancer chemoprevention. Breast Cancer Research and Treatment, 115: 223-239.
  • Yuri, T., Danbara, N., Tsujita-Kyutoku, M., Kiyozuka, Y., Senzaki, H., Shikata, N., Kanzaki, H., Tsubura, A. (2004). Perillyl alcohol inhibits human breast cancer cell growth in vitro and in vivo. Breast Cancer Research and Treatment, 84, 251–260.
  • Xu, M., Floyd, H.S., Greth, S.M., Chang, W.C.L., Lohman, K., Stoyanova, R., Kucera, G.L., Kute, T.E., Willingham, M.C., Miller, M.S. (2004). Perillyl alcohol-mediated inhibition of lung cancer cell line proliferation: potential mechanisms for its chemotherapeutic effects. Toxicology and Applied Pharmacology, 195, 232–246.
  • Yeruva, L., Pierre, K.J., Elegbede, A., Wang, R.C., Carper, SW. (2007). Perillyl alcohol and perillic acid induced cell cycle arrest and apoptosis in non small cell lung cancer cells. Cancer Letters. 257, 216–226.
  • Wiseman, D.A., Werner. S.R., Crowell, P.L. (2007). Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells. The Journal of pharmacology and experimental therapeutics, 320, 1163-1170.
  • Jayaprakasha, G,K., Murthy, K.N.C., Demarais, R., Patil, B.S. (2012). Inhibition of prostate cancer (LNCaP) cell proliferation by volatile components from Nagami kumquats. Planta Medica, 78, 974-980.
  • Goyary, D., Chattopadhyay, P., Giri, S., Aher, V., Upadhyay, A., Veer, V. (2014). Ochratoxin A induces cytotoxicity, DNA damage and apoptosis in rat hepatocyte primary cell culture at nanomolar concentration. World Mycotoxin Journal, 7, 379–386.
  • Riss, T.L., Moravec, R.A., Niles, A.L., Duellman, S., Benink, H.A., Worzella, T.J., Minor, L. (2016). Cell Viability Assays. Assay Guidance Manual, 1-31.
  • Ahmad, J., Alhadlaq, H.A., Alshamsan, A., Siddiqui, M.A., Saquib, Q., Khan, S.T., Wahab, R., Al-Khedhairy, A.A., Musarrat, J., Akhtar, M.J., Ahamed, M. (2016). Differential cytotoxicity of copper ferrite nanoparticles in different human cells. Journal of Applied Toxicology, 36(10), 1284-1293, doi: 10.1002/jat.3299.
  • Wattenberg, L.W. (1991). Inhibition of azoxymethane-induced neoplasia of the large bowel by 3-hydroxy-3,7,11-trimethyl-1,6,10-dodecatriene (nerolidol). Carcinogenesis, 12,151-152.
  • Lu, H.F., Liu, J.Y., Hsueh, S.C., Yang, Y.Y., Yang, J.S., Tan, T.W., Kok, L.F,. Lu, C.C., Lan, S.H., Wu, S.Y., Liao, S.S., Ip, S.W., Chung, J.G. (2007). (−)-Menthol inhibits WEHI-3 leukemia cells in vitro and in vivo,” In Vivo, 21 (2), 285–289.
  • Lin, J.P., Lu, H.F., Lee, J.H., Lin, J.G., Hsia, T.C., Wu, L.T., Chung, J.G. (2005). Menthol inhibits DNA topoisomerases I, II alpha and beta and promotes NF-kappaB expression in human gastric cancer SNU-5 cells. Anticancer Research, 25, 2069–2074.
  • Park, E,J., Kim, S,H., Kim, B,J., Kim, S.Y., So, I., Jeon, J.H. (2009). Menthol enhances an antiproliferative activity of 1𝛼,25-dihydroxyvitamin D3 in LNCaP cells. Journal of Clinical Biochemistry and Nutrition, 44 (2), 125–130.
  • Wang, Y., Wang, X., Yang, Z., Zhu, G., Chen, D., Meng, Z. (2005). Menthol inhibits the proliferation and motility of prostate cancer DU145 cells. Pathology Oncology Research. 18 (4), 903-910.
  • Jaafari, A., Mouse, H.A., Rakib, E.M., M’barek, L.A., Tilaoui, M., Benbakhta, C., Boulli, A., Abbad, A., Zyad, A. (2007). Chemical composition and antitumor activity of different wild varieties of Moroccan thyme. Revista Brasileira de Farmacognosia, 17, 477-491.
  • Slamenova, D., Horvathova, E., Sramkova, M., Marsalkova, L. (2007). DNA-protective effects of two components of essential plant oils carvacrol and thymol onmammaliancells cultured invitro. Neoplasma. 54, 108–112.
  • Jaafari, A., Tilaoui, M., Mouse, H.A., M’bark, L.A., Aboufatima, R., Chait, A., Lepoivre, M., Zyad, A. (2012). Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis. Revista Brasileira de Farmacognosia Brazilian Journal of Pharmacognosy, 22(3), 534-540.
  • Jin, X., Sun, J., Miao, X., Liu, G., Zhong, D. (2013). Inhibitory effect of geraniol in combination with gemcitabine on proliferation of BXPC-3 human pancreatic cancer cells. Journal of International Medical Research. 41(4) 993–1001.
  • Czarnecka, A.M., Golik. P., Bartnik, E. (2006). Mitochondrial DNA mutations in human neoplasia. Journal of Applied Genetics, 47, 67–78.

Investigation of Cytotoxic Effect of Monoterpenes Beta-Citronellol and (-)-Menthone in Human Breast Cancer (MCF-7) Cell Line

Year 2019, Volume: 3 Issue: 2, 111 - 119, 27.05.2019

Abstract

Objective: Monoterpenes
are the primary components of plant essential oils
which can use in medicines, food or cosmetic
industries.
Many terpenoids exhibit a various pharmacological activities such as
antiallergic,
antiviral,
anti-inflammatory and anticancer
. However no enough data are found about
cytotoxicity of these compounds on human breast cancer (MCF-7) cells.
The aim of this study was
to evaluate the potential cytotoxic effects of monoterpenes
Beta-Citronellol (BC) and (-)-Menthone (MNT) using
3-(4,5-dimethylthiazolyl-2)-2,5
diphenyl tetrazolium bromide (MTT)
assay in MCF-7 cell line.

Methods: In this study, the
cytotoxic effects of monoterpenes BC and MNT were analyzed by MTT assay at 24
and 48 hour treatment times in the MCF-7 cell line. MCF-7 c
ells were treated with different concentrations of BC (2, 10, 50, 250, 1250 μg/mL) and MNT (16, 80, 400, 2000, 10.000 μg/mL).

Results: BC and MNT were significantly decreased cell viability (%) at
all concentrations at 24-h treatment period.
Furthermore, BC (250, 1250 μg/mL) and MNT (16, 400,
2000, 10.000
μg/mL) were significantly reduced the cell viability at 48-h treatment. 







Conclusion: As a result, BC and MNT showed cytotoxic effect on MCF-7 cells in
both treatment times. Further studies
should be conducted to determination cytotoxicity using different cell lines. 

References

  • Gherlardini, C., Galeotti, N., Mazzanti, G. (2001). Local anaesthetic activity of monoterpenes and phenylpropanes of essential oils. Planta Medica, 67, 564–566.
  • Queiroz, T.B., Santos, G.F., Ventura, S.C., Hiruma-Lima, C.A., Gaivão, I.O.M., Maistro, E.L. (2017). Cytotoxic and genotoxic potential of geraniol in peripheral blood mononuclear cells and human hepatoma cell line (HepG2). Genetics and Molecular Research, 16 (3), gmr16039777, DOI http://dx.doi.org/10.4238/gmr16039777.
  • Bakkali, F., Averbeck, S., Averbeck, D., Idaomar, M. (2008). Biological effects of essential oils-a review. Food and Chemical Toxicology, 46, 446-475 https://doi.org/10.1016/j.fct.2007.09.106.
  • Slamenova, D., Horvathova, E., Wsolova, L., Sramkova, M., Navarova, J. (2009). Investigation of anti-oxidative, cytotoxic, DNA-damaging and DNA-protective effects of plant volatiles eugenol and borneol in human-derived HepG2, Caco-2 and VH10 cell lines. Mutation Research, 677, 46–52.
  • Unlu, M., Ergene, E., Unlu, G.V., Zeytinoglu, H.S., Vural, N. (2010). Composition, antimicrobial activity and in vitro cytotoxicity of essential oil from Cinnamomum zeylanicum Blume (Lauraceae). Food and Chemical Toxicology, 48, 3274–3280.
  • Balasundram, N., Sundram, K., Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry, 99(1), 191-203.
  • Alu’datt, M.H., Rababah, T., Alhamad, M.N., Al-Mahasneh, M.A., Almajwal, A., Gammoh, S., Ereifej, K., Johargy, A., Alli, I. (2017). A review of phenolic compounds in oil-bearing plants: Distribution, identification and occurrence of phenolic compounds. Food Chemistry, 218, 99–106.
  • Crowell, P.L. (1999). Prevention and therapy of cancer by diethary monoterpenes. The Journal of Nutrition, 129, 775S–778S.
  • Lampe, J.W. (2003). Spicing up a vegetarian diet: chemopreventive effects of phytochemicals. American Society for Clinical Nutrition, 78, 579S–583S.
  • Nikolic, B., Mitic-Culafic, D., Vukovic-Gacic, B., Knezevic-Vukcevic, J. (2011). Modulation of genotoxicity and DNA repair by plant monoterpenes camphor, eucalyptol and thujone in Escherichia coli and mammalian cells. Food and Chemical Toxicology, 49, 2035-2045.
  • Ribeiro-Filho, H.V., Silva de, C.M.S., Siqueira de, R.B., Lahlou, S., Santos, A.A., Magalhães, P.J.C. (2016). Biphasic cardiovascularandrespiratoryeffectsinducedby β-citronellol. European Journal of Pharmacology. 775, 96–105.
  • Elsharif, S.A., Buettner, A. (2017). Influence of the chemical structure on the odor characters of β-citronellol and its oxygenated derivatives. Food Chemistry, 232, 704–711.
  • Murakami, A., Furukawa, J., Kawasaki, Y., Ota, R. (2013). Flavoring agent with natural fruit-like hop aroma and manufacturing method of beverages. In: Kirin Brewery Co., Ltd. Japan. pp. 19.
  • Brito, R.G., Guimarães, A.G., Quintans, J.S.S., Santos, M.R.V., Sousa, D.P., Badaue-Passos, D., Quintans, L.J. (2012). Citronellol, a monoterpene alcohol, reduces nociceptive and inflammatory activities in rodents. Journal of Natural Medicines, 66 (4), 637–644.
  • Kamatou, G.P.P., Vermaak, I., Viljoen, A.M., Lawrence, B.M. (2013). Menthol: A simple monoterpene with remarkable biological properties. Phytochemistry, 96, 15–25.
  • Croteau, R.B., Davis, E.M., Ringer, K.L., Wildung, M.R. (2005). (-)-Menthol biosynthesis and molecular genetics. Naturwissenschaften, 92, 562–577.
  • Lawrence, B.M. (2013). The story of India’s mint oils and menthol. Perfumer and Flavorist, 38 (1), 26–35.
  • Thoppil, R.J., Bishayee, A. (2011). Terpenoids as potential chemopreventive and therapeutic agents in liver cancer. World journal of hepatology, 3, 228-249.
  • Murthy, K.N.C., Jayaprakasha, G.K., Patil, B.S. (2012). D-limonene rich volatile oil from blood oranges inhibits angiogenesis, metastasis and cell death in human colon cancer cells. Life Sciences, 91, 429- 439.
  • Steward, W.P., Brown, K. (2013). Cancer chemoprevention: a rapidly evolving field. British Journal of Cancer, 109, 1-7.
  • Maßberg, D., Simon, A., Häussinger, D., Keitel, V., Gisselmann, G., Conrad, H., Hatt, H. (2015). Monoterpene (-)-citronellal affects hepatocarcinoma cell signaling via an olfactory receptor. Archives of Biochemistry and Biophysics, 566, 100–109.
  • Meng, C., He, Y., Wei, Z., Lu, Y., Du, F., Ou, G., Wang, N., Luo, X.G., Ma, W. (2018). Zhang TC. He H. MRTF-A mediates the activation of COL1A1 expression stimulated by multiple signaling pathways in human breast cancer cells. Biomedicine & Pharmacotherapy, 104, 718-728.
  • Song, Y., Lu, M., Qiu, H., Yin, J., Luo, K., Zhang, Z., Jia, X., Zheng, G., Liu, H., He, Z. (2018). Activation of FOXO3a reverses 5-Fluorouracil resistance in human breast cancer cells. Experimental and Molecular Pathology, 105, 57–62.
  • https://www.sigmaaldrich.com/catalog/product/aldrich/c83201?lang=en&region=TR
  • https://www.sigmaaldrich.com/catalog/product/aldrich/218235?lang=en&region=TR
  • Mossman, T. (1983). Rapid colometric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65, 55-63.
  • Mamur, S., Yuzbasioglu, D., Yılmaz, S., Erikel, E., Unal, F. (2018). Assessment of Cytotoxic and Genotoxic Effects of Enniatin-A In Vitro. Food Additives & Contaminants: Part A, 35 (8), 1633-1644, https://doi.org/10.1080/19440049.2018.1486513.
  • Liu, S.J., Liao, Z.X., Liu, C., Sun, J.Y. (2015). Chemical constituents, anticancer, antimicrobial and antioxidant activities of essential oil from Anaphalis lacteal grown in Qinghai-Tibet Plateau. Natural Product Research, 29, 1369-1371.
  • Rabi, T., Bishayee, A. (2009). Terpenoids and breast cancer chemoprevention. Breast Cancer Research and Treatment, 115: 223-239.
  • Yuri, T., Danbara, N., Tsujita-Kyutoku, M., Kiyozuka, Y., Senzaki, H., Shikata, N., Kanzaki, H., Tsubura, A. (2004). Perillyl alcohol inhibits human breast cancer cell growth in vitro and in vivo. Breast Cancer Research and Treatment, 84, 251–260.
  • Xu, M., Floyd, H.S., Greth, S.M., Chang, W.C.L., Lohman, K., Stoyanova, R., Kucera, G.L., Kute, T.E., Willingham, M.C., Miller, M.S. (2004). Perillyl alcohol-mediated inhibition of lung cancer cell line proliferation: potential mechanisms for its chemotherapeutic effects. Toxicology and Applied Pharmacology, 195, 232–246.
  • Yeruva, L., Pierre, K.J., Elegbede, A., Wang, R.C., Carper, SW. (2007). Perillyl alcohol and perillic acid induced cell cycle arrest and apoptosis in non small cell lung cancer cells. Cancer Letters. 257, 216–226.
  • Wiseman, D.A., Werner. S.R., Crowell, P.L. (2007). Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells. The Journal of pharmacology and experimental therapeutics, 320, 1163-1170.
  • Jayaprakasha, G,K., Murthy, K.N.C., Demarais, R., Patil, B.S. (2012). Inhibition of prostate cancer (LNCaP) cell proliferation by volatile components from Nagami kumquats. Planta Medica, 78, 974-980.
  • Goyary, D., Chattopadhyay, P., Giri, S., Aher, V., Upadhyay, A., Veer, V. (2014). Ochratoxin A induces cytotoxicity, DNA damage and apoptosis in rat hepatocyte primary cell culture at nanomolar concentration. World Mycotoxin Journal, 7, 379–386.
  • Riss, T.L., Moravec, R.A., Niles, A.L., Duellman, S., Benink, H.A., Worzella, T.J., Minor, L. (2016). Cell Viability Assays. Assay Guidance Manual, 1-31.
  • Ahmad, J., Alhadlaq, H.A., Alshamsan, A., Siddiqui, M.A., Saquib, Q., Khan, S.T., Wahab, R., Al-Khedhairy, A.A., Musarrat, J., Akhtar, M.J., Ahamed, M. (2016). Differential cytotoxicity of copper ferrite nanoparticles in different human cells. Journal of Applied Toxicology, 36(10), 1284-1293, doi: 10.1002/jat.3299.
  • Wattenberg, L.W. (1991). Inhibition of azoxymethane-induced neoplasia of the large bowel by 3-hydroxy-3,7,11-trimethyl-1,6,10-dodecatriene (nerolidol). Carcinogenesis, 12,151-152.
  • Lu, H.F., Liu, J.Y., Hsueh, S.C., Yang, Y.Y., Yang, J.S., Tan, T.W., Kok, L.F,. Lu, C.C., Lan, S.H., Wu, S.Y., Liao, S.S., Ip, S.W., Chung, J.G. (2007). (−)-Menthol inhibits WEHI-3 leukemia cells in vitro and in vivo,” In Vivo, 21 (2), 285–289.
  • Lin, J.P., Lu, H.F., Lee, J.H., Lin, J.G., Hsia, T.C., Wu, L.T., Chung, J.G. (2005). Menthol inhibits DNA topoisomerases I, II alpha and beta and promotes NF-kappaB expression in human gastric cancer SNU-5 cells. Anticancer Research, 25, 2069–2074.
  • Park, E,J., Kim, S,H., Kim, B,J., Kim, S.Y., So, I., Jeon, J.H. (2009). Menthol enhances an antiproliferative activity of 1𝛼,25-dihydroxyvitamin D3 in LNCaP cells. Journal of Clinical Biochemistry and Nutrition, 44 (2), 125–130.
  • Wang, Y., Wang, X., Yang, Z., Zhu, G., Chen, D., Meng, Z. (2005). Menthol inhibits the proliferation and motility of prostate cancer DU145 cells. Pathology Oncology Research. 18 (4), 903-910.
  • Jaafari, A., Mouse, H.A., Rakib, E.M., M’barek, L.A., Tilaoui, M., Benbakhta, C., Boulli, A., Abbad, A., Zyad, A. (2007). Chemical composition and antitumor activity of different wild varieties of Moroccan thyme. Revista Brasileira de Farmacognosia, 17, 477-491.
  • Slamenova, D., Horvathova, E., Sramkova, M., Marsalkova, L. (2007). DNA-protective effects of two components of essential plant oils carvacrol and thymol onmammaliancells cultured invitro. Neoplasma. 54, 108–112.
  • Jaafari, A., Tilaoui, M., Mouse, H.A., M’bark, L.A., Aboufatima, R., Chait, A., Lepoivre, M., Zyad, A. (2012). Comparative study of the antitumor effect of natural monoterpenes: relationship to cell cycle analysis. Revista Brasileira de Farmacognosia Brazilian Journal of Pharmacognosy, 22(3), 534-540.
  • Jin, X., Sun, J., Miao, X., Liu, G., Zhong, D. (2013). Inhibitory effect of geraniol in combination with gemcitabine on proliferation of BXPC-3 human pancreatic cancer cells. Journal of International Medical Research. 41(4) 993–1001.
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There are 47 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Sevcan Mamur 0000-0002-8615-5331

Publication Date May 27, 2019
Published in Issue Year 2019 Volume: 3 Issue: 2

Cite

APA Mamur, S. (2019). Investigation of Cytotoxic Effect of Monoterpenes Beta-Citronellol and (-)-Menthone in Human Breast Cancer (MCF-7) Cell Line. Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi, 3(2), 111-119.