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Investigation of the antifungal activity of lichen (Usnea longissima) extracts against Fusarium graminearum

Year 2022, Volume: 6 Issue: 2, 104 - 108, 15.11.2022

Asmar Taghiyeva Ayşe Feyza Tufan Dülger Emre Yörük Tubanur Aslan Engin

https://doi.org/10.30616/ajb.1172113
Cited By: 1

Abstract

The aim of this study was to investigate the antifungal effects and molecular changes caused by Usnea longissima Ach. extracts against Fusarium greaminearum. In agar well diffusion assay, the zone of inhibition increased as the concentration increased in both of methanol and acetone extracts (1, 10, 20 and 50 mg/ml). In terms of bioactivities, 1 mg/ml was active, while other concentrations were very active. At the molecular level, changes caused by 50 mg/ml methanol extract was analyzed by qPCR with terms of cat, mst20, and tri5 genes, which are associated with antioxidation, apoptosis, and trichothecene production, respectively. Transcript levels of tri5 decreased (0.29 fold) while cat (2.41 fold) and mst20 (1.48 fold) increased. Findings from this study showed that U. longissima extracts could be natural antifungal agent against worldwide phytopathogen F. graminearum.

Keywords

Agar well diffusion assay Fusarium graminearum gene expression Usnea longissima

References

  • Aslan A, Gurbuz H, Yazici K, Cicek A, Turan M, Ercisli S (2013). Evaluation of lichens as bio-indicators of metal pollution. Journal of Elementology 18(3): 353-369.
  • Aydin S, Kinalioğlu K, Sökmen BB (2018). Antioxidant, anti-urease and anti-elastase activities of Usnea longissima Ach. Bangladesh Journal of Botany 47(3): 429-435.
  • Bharti S, Nayaka S, Kumar R (2022). Evaluation of some traditional therapeutic properties of Usnea longissima (Ascomycota, lichenized fungi): antimicrobial, antiquorum and antioxidant. Journal of Microbiology, Biotechnology and Food Sciences 11(4): e3163.
  • Brisdelli F, Perilli M, Sellitri D, Piovano M, Garbarino JA, Nicoletti M, Bozzi A, Amicosante G, Celenza G (2013). Cytotoxic activity and antioxidant capacity of purified lichen metabolites: an in vitro study. Phytotherapy Research 27(3): 431-437.
  • Dandapat M, Paul S (2019). Secondary metabolites from lichen Usnea longissima and its pharmacological relevance. Pharmacognosy Research 11(2): 103-109.
  • Desjardin AE (2007). Fusarium mycotoxins chemistry, genetics and biology. Plant Pathology 56(2): 336-337.
  • Devashree AP, Dikshit A, Nayaka S (2019). Antimicrobial role of Usnea longissima against pathogenic microorganisms. International Journal of Scientific. Engineering Research (10)5: 157-167.
  • Furmanek L, Czarnota P, Seaward MR (2022). A review of the potential of lichen substances as antifungal agents: the effects of extracts and lichen secondary metabolites on Fusarium fungi. Archives of Microbiology 204(8): 1-31.
  • Gazdağlı A, Sefer Ö, Yörük E, Varol Gİ, Teker T, Albayrak G (2018). Investigation of camphor effects on Fusarium graminearum and F. culmorum at different molecular levels. Pathogens 7(4): 90.
  • Gazo SMT, Santiago KAA, Tjitrosoedirjo SS, Cruz TEE (2019). Antimicrobial and herbicidal activities of the fruticose lichen Ramalina from Guimaras Island, Philippines. Biotropia 26(1): 23-32.
  • Goel M, Sharma PK, Dureja P, Rani A, Uniyal PL (2011). Antifungal activity of extracts of the lichens Parmelia reticulata, Ramalina roesleri, Usnea longissima and Stereocaulon himalayense. Archives of Phytopathology and Plant Protection 44(13): 1300-1311.
  • Halama P, Van HC (2004). Antifungal activity of lichen extracts and lichenic acids. BioControl 49(1): 95-107.
  • Halıcı M, Odabasoglu F, Suleyman H, Cakir A, Aslan A, Bayir Y (2005). Effects of water extract of Usnea longissima on antioxidant enzyme activity and mucosal damage caused by indomethacin in rats. Phytomedicine 12(9): 656-662.
  • Hawksworth D, Grube M (2020). Lichens redefined as complex ecosystems. The New Phytologist 227(5): 1281-1283.
  • Joulain D, Tabacchi R (2009). Lichen extracts as raw materials in perfumery. Part 1: oakmoss. Flavour and Fragrance Journal 24(2): 49-61.
  • Koch E (1999). Evaluation of commercial products for microbial control of soil-borne plant diseases. Crop Protection 18(2): 119-125.
  • Lacey LA, Liu TX, Buchman JL, Munyaneza JE, Goolsby JA, Horton DR (2011). Entomopathogenic fungi (Hypocreales) for control of potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae) in an area endemic for zebra chip disease of potato. Biological Control 56(3): 271-278.
  • Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25(4): 402-408.
  • Mielniczuk E, Skwaryło-Bednarz B (2020). Fusarium head blight, mycotoxins and strategies for their reduction. Agronomy 10(4): 509.
  • Nalam VJ, Sarowar S, Shah J (2016). Establishment of a Fusarium graminearum infection model in Arabidopsis thaliana leaves and floral tissues. Bio-protocol 6(14): 1877.
  • Osborne LE, Stein JM (2007). Epidemiology of Fusarium head blight on small-grain cereals. International Journal of Food Microbiology 119(1-2): 103-108.
  • Pamenta AFA, Azis T, Watoni AH, Natsir M, Ansharullahi Adiba IF, Dermawan A, Nurdin M, Maulidiyah (2020). Antimalarial activity of lichen Usnea longissima. In AIP Conference Proceedings 2243: 020017.
  • Parry DW, Jenkinson P, McLeod L (1995). Fusarium ear blight (scab) in small-grain cereals. Plant Pathology 44(2): 207-238.
  • Quinto E, Santos M (2005). A guidebook to plant screening: phytochemical and biological. Research Center for the Natural Sciences, University of Santo Tomas.
  • Reddy SD, Siva B, Kumar K, Babu VP, Sravanthi V, Bousti, J, Babu KS (2019). Comprehensive analysis of secondary metabolites in Usnea longissima (lichenized ascomycetes, Parmeliaceae) using uplc-esi-qtof-ms/ms and pro-apoptotic activity of barbatic acid. Molecules 24(12): 2270.
  • Shao J, Pei Z, Jing H, Wang L, Jiang C, Du X, Wang H (2021). Antifungal activity of myriocin against Fusarium graminearum and its inhibitory effect on deoxynivalenol production in wheat grains. Physiological and Molecular Plant Pathology 114: 101635.
  • Sharma M, Mohammad A (2020). Lichens and lichenology: Historical and economic prospects. Lichen‐Derived Products: Extraction and Applications 101-118.
  • Shukla V, Joshi GP, Rawat MSM (2010). Lichens as a potential natural source of bioactive compounds: a review. Phytochemistry reviews 9(2): 303-314.
  • Snijders CHA (1990). Fusarium head blight and mycotoxin contamination of wheat, a review. Netherlands Journal of Plant Pathology 96(4): 187-198.
  • Teker T, Sefer Ö, Gazdağlı A, Yörük E, Varol Gİ, Albayrak G (2021). α-Thujone exhibits an antifungal activity against F. graminearum by inducing oxidative stress, apoptosis, epigenetics alterations and reduced toxin synthesis. European Journal of Plant Pathology 160(3): 611-622.
  • Tiwari P, Rai H, Upreti DK, Trivedi S, Shukla P (2011a). Assessment of antifungal activity of some himalayan foliose lichens against plant pathogenic fungi. American Journal of Plant Sciences 2(6): 841-846.
  • Tiwari P, Rai H, Upreti DK, Trivedi S, Shukla P (2011b). Antifungal activity of a common Himalayan foliose lichen Parmotrema tinctorum (Despr. ex Nyl.) Hale. Nature and Science 9(9): 167-171.
  • Upreti DK, Divakar PK, Nayaka S (2005). Commercial and ethnic use of lichens in India. Economic Botany 59(3): 269-273.
  • Warrior P, Rehberger LA, Beach M, Grau PA, Kirfman GW, Conley JM (1999). Commercial development and introduction of DiTeraTM, a new nematicide. Pesticide Science 55(3): 376-379.
  • Xiu Q, Bi L, Xu H, Li T, Zhou Z, Li Z, Zhou M (2021). Antifungal activity of quinofumelin against Fusarium graminearum and its inhibitory effect on DON biosynthesis. Toxins 13(5): 348.
  • Yadav H, Nayak S, Dwivedi M (2021). Analytics on antimicrobial activity of lichen extract. Journal of Pure and Applied Microbiology 15(2): 701-708.
  • Yee WL, Toscano NC (1998). Laboratory evaluations of synthetic and natural insecticides on beet armyworm (Lepidoptera: Noctuidae) damage and survival on lettuce. Journal of Economic Entomology 91(1): 56-63.
  • Yıldırım E, Aslan A, Emsen B, Cakir A, Ercisli S (2012). Insecticidal effect of Usnea longissima (Parmeliaceae) extract against Sitophilus granarius (Coleoptera: Curculionidae). International Journal of Agriculture and Biology 14(2): 303-306.
  • Yörük E, Özkale E, Sefer Ö, Özsoy E (2022). Trichoderma atroviride triggers alterations at epigenetics, transcripts, oxidative stress, and apoptosis levels on Fusarium graminearum. Journal of Plant Pathology 104:1039-1047.
  • Yuen GY, Schoneweis SD (2007). Strategies for managing Fusarium head blight and deoxynivalenol accumulation in wheat. International Journal of Food Microbiology 119(1-2): 126-130.

Liken (Usnea longissima) ekstraktlarının Fusarium graminearum’a karşı antifungal aktivitesinin araştırılması

Year 2022, Volume: 6 Issue: 2, 104 - 108, 15.11.2022

Asmar Taghiyeva Ayşe Feyza Tufan Dülger Emre Yörük Tubanur Aslan Engin

https://doi.org/10.30616/ajb.1172113
Cited By: 1

Abstract

Bu çalışmanın amacı, Usnea longissima ekstraktlarının Fusarium greaminearum’a karşı neden olduğu antifungal etkileri ve moleküler değişiklikleri araştırmaktır. Agar kuyu difüzyon testinde, hem metanol hem de aseton özütlerinde (1, 10, 20 ve 50 mg/ml) konsantrasyon arttıkça inhibisyon bölgesi arttı. Biyoaktiviteler açısından bakıldığında, 1 mg/ml konsatrasyon aktifken, diğer konsantrasyonlar çok aktifti. Moleküler düzeyde, 50 mg/ml metanol özütünün neden olduğu değişiklikler, sırasıyla antioksidasyon, apoptoz ve trikotesen üretimi ile ilişkili cat, mst20 ve tri5 genleri açısından qPCR ile analiz edildi. tri5’in transkript seviyeleri azalırken (0.29 kat), cat (2.41 kat) ve mst20 (1.48 kat) arttı. Bu çalışmadan elde edilen bulgular, U. longissima ekstraktlarının dünya çapında fitopatojen F. graminearum’a karşı doğal antifungal ajan olabileceğini göstermektedir.

Keywords

Agar kuyu difüzyon testi Fusarium graminearum gen ekspresyonu Usnea longissima

References

  • Aslan A, Gurbuz H, Yazici K, Cicek A, Turan M, Ercisli S (2013). Evaluation of lichens as bio-indicators of metal pollution. Journal of Elementology 18(3): 353-369.
  • Aydin S, Kinalioğlu K, Sökmen BB (2018). Antioxidant, anti-urease and anti-elastase activities of Usnea longissima Ach. Bangladesh Journal of Botany 47(3): 429-435.
  • Bharti S, Nayaka S, Kumar R (2022). Evaluation of some traditional therapeutic properties of Usnea longissima (Ascomycota, lichenized fungi): antimicrobial, antiquorum and antioxidant. Journal of Microbiology, Biotechnology and Food Sciences 11(4): e3163.
  • Brisdelli F, Perilli M, Sellitri D, Piovano M, Garbarino JA, Nicoletti M, Bozzi A, Amicosante G, Celenza G (2013). Cytotoxic activity and antioxidant capacity of purified lichen metabolites: an in vitro study. Phytotherapy Research 27(3): 431-437.
  • Dandapat M, Paul S (2019). Secondary metabolites from lichen Usnea longissima and its pharmacological relevance. Pharmacognosy Research 11(2): 103-109.
  • Desjardin AE (2007). Fusarium mycotoxins chemistry, genetics and biology. Plant Pathology 56(2): 336-337.
  • Devashree AP, Dikshit A, Nayaka S (2019). Antimicrobial role of Usnea longissima against pathogenic microorganisms. International Journal of Scientific. Engineering Research (10)5: 157-167.
  • Furmanek L, Czarnota P, Seaward MR (2022). A review of the potential of lichen substances as antifungal agents: the effects of extracts and lichen secondary metabolites on Fusarium fungi. Archives of Microbiology 204(8): 1-31.
  • Gazdağlı A, Sefer Ö, Yörük E, Varol Gİ, Teker T, Albayrak G (2018). Investigation of camphor effects on Fusarium graminearum and F. culmorum at different molecular levels. Pathogens 7(4): 90.
  • Gazo SMT, Santiago KAA, Tjitrosoedirjo SS, Cruz TEE (2019). Antimicrobial and herbicidal activities of the fruticose lichen Ramalina from Guimaras Island, Philippines. Biotropia 26(1): 23-32.
  • Goel M, Sharma PK, Dureja P, Rani A, Uniyal PL (2011). Antifungal activity of extracts of the lichens Parmelia reticulata, Ramalina roesleri, Usnea longissima and Stereocaulon himalayense. Archives of Phytopathology and Plant Protection 44(13): 1300-1311.
  • Halama P, Van HC (2004). Antifungal activity of lichen extracts and lichenic acids. BioControl 49(1): 95-107.
  • Halıcı M, Odabasoglu F, Suleyman H, Cakir A, Aslan A, Bayir Y (2005). Effects of water extract of Usnea longissima on antioxidant enzyme activity and mucosal damage caused by indomethacin in rats. Phytomedicine 12(9): 656-662.
  • Hawksworth D, Grube M (2020). Lichens redefined as complex ecosystems. The New Phytologist 227(5): 1281-1283.
  • Joulain D, Tabacchi R (2009). Lichen extracts as raw materials in perfumery. Part 1: oakmoss. Flavour and Fragrance Journal 24(2): 49-61.
  • Koch E (1999). Evaluation of commercial products for microbial control of soil-borne plant diseases. Crop Protection 18(2): 119-125.
  • Lacey LA, Liu TX, Buchman JL, Munyaneza JE, Goolsby JA, Horton DR (2011). Entomopathogenic fungi (Hypocreales) for control of potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae) in an area endemic for zebra chip disease of potato. Biological Control 56(3): 271-278.
  • Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25(4): 402-408.
  • Mielniczuk E, Skwaryło-Bednarz B (2020). Fusarium head blight, mycotoxins and strategies for their reduction. Agronomy 10(4): 509.
  • Nalam VJ, Sarowar S, Shah J (2016). Establishment of a Fusarium graminearum infection model in Arabidopsis thaliana leaves and floral tissues. Bio-protocol 6(14): 1877.
  • Osborne LE, Stein JM (2007). Epidemiology of Fusarium head blight on small-grain cereals. International Journal of Food Microbiology 119(1-2): 103-108.
  • Pamenta AFA, Azis T, Watoni AH, Natsir M, Ansharullahi Adiba IF, Dermawan A, Nurdin M, Maulidiyah (2020). Antimalarial activity of lichen Usnea longissima. In AIP Conference Proceedings 2243: 020017.
  • Parry DW, Jenkinson P, McLeod L (1995). Fusarium ear blight (scab) in small-grain cereals. Plant Pathology 44(2): 207-238.
  • Quinto E, Santos M (2005). A guidebook to plant screening: phytochemical and biological. Research Center for the Natural Sciences, University of Santo Tomas.
  • Reddy SD, Siva B, Kumar K, Babu VP, Sravanthi V, Bousti, J, Babu KS (2019). Comprehensive analysis of secondary metabolites in Usnea longissima (lichenized ascomycetes, Parmeliaceae) using uplc-esi-qtof-ms/ms and pro-apoptotic activity of barbatic acid. Molecules 24(12): 2270.
  • Shao J, Pei Z, Jing H, Wang L, Jiang C, Du X, Wang H (2021). Antifungal activity of myriocin against Fusarium graminearum and its inhibitory effect on deoxynivalenol production in wheat grains. Physiological and Molecular Plant Pathology 114: 101635.
  • Sharma M, Mohammad A (2020). Lichens and lichenology: Historical and economic prospects. Lichen‐Derived Products: Extraction and Applications 101-118.
  • Shukla V, Joshi GP, Rawat MSM (2010). Lichens as a potential natural source of bioactive compounds: a review. Phytochemistry reviews 9(2): 303-314.
  • Snijders CHA (1990). Fusarium head blight and mycotoxin contamination of wheat, a review. Netherlands Journal of Plant Pathology 96(4): 187-198.
  • Teker T, Sefer Ö, Gazdağlı A, Yörük E, Varol Gİ, Albayrak G (2021). α-Thujone exhibits an antifungal activity against F. graminearum by inducing oxidative stress, apoptosis, epigenetics alterations and reduced toxin synthesis. European Journal of Plant Pathology 160(3): 611-622.
  • Tiwari P, Rai H, Upreti DK, Trivedi S, Shukla P (2011a). Assessment of antifungal activity of some himalayan foliose lichens against plant pathogenic fungi. American Journal of Plant Sciences 2(6): 841-846.
  • Tiwari P, Rai H, Upreti DK, Trivedi S, Shukla P (2011b). Antifungal activity of a common Himalayan foliose lichen Parmotrema tinctorum (Despr. ex Nyl.) Hale. Nature and Science 9(9): 167-171.
  • Upreti DK, Divakar PK, Nayaka S (2005). Commercial and ethnic use of lichens in India. Economic Botany 59(3): 269-273.
  • Warrior P, Rehberger LA, Beach M, Grau PA, Kirfman GW, Conley JM (1999). Commercial development and introduction of DiTeraTM, a new nematicide. Pesticide Science 55(3): 376-379.
  • Xiu Q, Bi L, Xu H, Li T, Zhou Z, Li Z, Zhou M (2021). Antifungal activity of quinofumelin against Fusarium graminearum and its inhibitory effect on DON biosynthesis. Toxins 13(5): 348.
  • Yadav H, Nayak S, Dwivedi M (2021). Analytics on antimicrobial activity of lichen extract. Journal of Pure and Applied Microbiology 15(2): 701-708.
  • Yee WL, Toscano NC (1998). Laboratory evaluations of synthetic and natural insecticides on beet armyworm (Lepidoptera: Noctuidae) damage and survival on lettuce. Journal of Economic Entomology 91(1): 56-63.
  • Yıldırım E, Aslan A, Emsen B, Cakir A, Ercisli S (2012). Insecticidal effect of Usnea longissima (Parmeliaceae) extract against Sitophilus granarius (Coleoptera: Curculionidae). International Journal of Agriculture and Biology 14(2): 303-306.
  • Yörük E, Özkale E, Sefer Ö, Özsoy E (2022). Trichoderma atroviride triggers alterations at epigenetics, transcripts, oxidative stress, and apoptosis levels on Fusarium graminearum. Journal of Plant Pathology 104:1039-1047.
  • Yuen GY, Schoneweis SD (2007). Strategies for managing Fusarium head blight and deoxynivalenol accumulation in wheat. International Journal of Food Microbiology 119(1-2): 126-130.
There are 40 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Articles
Authors

Asmar Taghiyeva 0000-0001-7637-7400

Ayşe Feyza Tufan Dülger 0000-0003-4779-6811

Emre Yörük 0000-0003-2770-0157

Tubanur Aslan Engin 0000-0003-2885-8829

Publication Date November 15, 2022
Acceptance Date September 25, 2022
Published in Issue Year 2022 Volume: 6 Issue: 2

Cite

APA Taghiyeva, A., Tufan Dülger, A. F., Yörük, E., Aslan Engin, T. (2022). Investigation of the antifungal activity of lichen (Usnea longissima) extracts against Fusarium graminearum. Anatolian Journal of Botany, 6(2), 104-108. https://doi.org/10.30616/ajb.1172113
AMA Taghiyeva A, Tufan Dülger AF, Yörük E, Aslan Engin T. Investigation of the antifungal activity of lichen (Usnea longissima) extracts against Fusarium graminearum. Ant J Bot. November 2022;6(2):104-108. doi:10.30616/ajb.1172113
Chicago Taghiyeva, Asmar, Ayşe Feyza Tufan Dülger, Emre Yörük, and Tubanur Aslan Engin. “Investigation of the Antifungal Activity of Lichen (Usnea Longissima) Extracts Against Fusarium Graminearum”. Anatolian Journal of Botany 6, no. 2 (November 2022): 104-8. https://doi.org/10.30616/ajb.1172113.
EndNote Taghiyeva A, Tufan Dülger AF, Yörük E, Aslan Engin T (November 1, 2022) Investigation of the antifungal activity of lichen (Usnea longissima) extracts against Fusarium graminearum. Anatolian Journal of Botany 6 2 104–108.
IEEE A. Taghiyeva, A. F. Tufan Dülger, E. Yörük, and T. Aslan Engin, “Investigation of the antifungal activity of lichen (Usnea longissima) extracts against Fusarium graminearum”, Ant J Bot, vol. 6, no. 2, pp. 104–108, 2022, doi: 10.30616/ajb.1172113.
ISNAD Taghiyeva, Asmar et al. “Investigation of the Antifungal Activity of Lichen (Usnea Longissima) Extracts Against Fusarium Graminearum”. Anatolian Journal of Botany 6/2 (November 2022), 104-108. https://doi.org/10.30616/ajb.1172113.
JAMA Taghiyeva A, Tufan Dülger AF, Yörük E, Aslan Engin T. Investigation of the antifungal activity of lichen (Usnea longissima) extracts against Fusarium graminearum. Ant J Bot. 2022;6:104–108.
MLA Taghiyeva, Asmar et al. “Investigation of the Antifungal Activity of Lichen (Usnea Longissima) Extracts Against Fusarium Graminearum”. Anatolian Journal of Botany, vol. 6, no. 2, 2022, pp. 104-8, doi:10.30616/ajb.1172113.
Vancouver Taghiyeva A, Tufan Dülger AF, Yörük E, Aslan Engin T. Investigation of the antifungal activity of lichen (Usnea longissima) extracts against Fusarium graminearum. Ant J Bot. 2022;6(2):104-8.

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Geçmişten günümüze potansiyel hammadde kaynağı: Likenler
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